Cervicovaginal levels of human beta defensins during bacterial vaginosis
Autor(a) principal: | |
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Data de Publicação: | 2021 |
Outros Autores: | , , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1371/journal.pone.0260753 http://hdl.handle.net/11449/230013 |
Resumo: | Aims To compare the cervicovaginal levels of human beta defensin (hBD)-1, 2 and 3 of women according to the status of Nugent-defined bacterial vaginosis (BV). Methods A total of 634 women of reproductive age were included in the study. Participants were equally distributed in two groups: according to the classification of vaginal smears according to Nugent criteria in normal (scores 0 to 3) and BV (scores ≥7). Cervicovaginal fluid samples were used for measurements of hBDs1, 2 and 3 levels by enzyme-linked immunosorbent assay (ELISA). Levels of each hBD were compared between the two study groups using Mann-Whitney test, with p-value <0.05 considered as significant. Odds ratio (OR) and 95% confidence interval (95% CI) were calculated for sociodemographic variables and hBD1-3 levels associated with BV a multivariable analysis. Correlation between Nugent score and measured levels of hBDs1-3 were calculated using Spearman’s test. Results Cervicovaginal fluids from women with BV showed lower levels of hBD-1 [median 2,400.00 pg/mL (0–27,800.00); p<0.0001], hBD-2 [5,600.00 pg/mL (0–45,800.00); p<0.0001] and hBD-3 [1,600.00 pg/mL (0–81,700.00); p = 0.012] when compared to optimal microbiota [hBD-1: [median 3,400.00 pg/mL (0–35,600.00), hBD-2: 12,300.00 pg/mL (0–95,300.00) and hBD-3: 3,000.00 pg/mL (0–64,300.00), respectively]. Multivariable analysis showed that lower levels of hBD-1 (OR: 2.05; 95% CI: 1.46–2.87), hBD-2 (OR: 1.85; 95% CI: 1.32–2.60) and hBD-3 (OR: 1.90; 95% CI: 1.37–2.64) were independently associated BV. Significant negative correlations were observed between Nugent scores and cervicovaginal levels of hBD-1 (Spearman’s rho = -0.2118; p = 0.0001) and hBD-2 (*Spearman’s rho = -0.2117; p = 0.0001). Conclusions Bacterial vaginosis is associated with lower cervicovaginal levels of hBDs1-3 in reproductive-aged women. |
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Cervicovaginal levels of human beta defensins during bacterial vaginosisAims To compare the cervicovaginal levels of human beta defensin (hBD)-1, 2 and 3 of women according to the status of Nugent-defined bacterial vaginosis (BV). Methods A total of 634 women of reproductive age were included in the study. Participants were equally distributed in two groups: according to the classification of vaginal smears according to Nugent criteria in normal (scores 0 to 3) and BV (scores ≥7). Cervicovaginal fluid samples were used for measurements of hBDs1, 2 and 3 levels by enzyme-linked immunosorbent assay (ELISA). Levels of each hBD were compared between the two study groups using Mann-Whitney test, with p-value <0.05 considered as significant. Odds ratio (OR) and 95% confidence interval (95% CI) were calculated for sociodemographic variables and hBD1-3 levels associated with BV a multivariable analysis. Correlation between Nugent score and measured levels of hBDs1-3 were calculated using Spearman’s test. Results Cervicovaginal fluids from women with BV showed lower levels of hBD-1 [median 2,400.00 pg/mL (0–27,800.00); p<0.0001], hBD-2 [5,600.00 pg/mL (0–45,800.00); p<0.0001] and hBD-3 [1,600.00 pg/mL (0–81,700.00); p = 0.012] when compared to optimal microbiota [hBD-1: [median 3,400.00 pg/mL (0–35,600.00), hBD-2: 12,300.00 pg/mL (0–95,300.00) and hBD-3: 3,000.00 pg/mL (0–64,300.00), respectively]. Multivariable analysis showed that lower levels of hBD-1 (OR: 2.05; 95% CI: 1.46–2.87), hBD-2 (OR: 1.85; 95% CI: 1.32–2.60) and hBD-3 (OR: 1.90; 95% CI: 1.37–2.64) were independently associated BV. Significant negative correlations were observed between Nugent scores and cervicovaginal levels of hBD-1 (Spearman’s rho = -0.2118; p = 0.0001) and hBD-2 (*Spearman’s rho = -0.2117; p = 0.0001). Conclusions Bacterial vaginosis is associated with lower cervicovaginal levels of hBDs1-3 in reproductive-aged women.Department of Pathology Botucatu Medical School São Paulo State University (UNESP)Graduate Program in Biomedical Sciences Medical School Universidade Federal da Fronteira Sul (UFFS), RSDepartment of Basic Pathology Setor de Ciências Biológicas Universidade Federal do Paraná (UFPR)Department of Pathology Botucatu Medical School São Paulo State University (UNESP)Universidade Estadual Paulista (UNESP)Universidade Federal da Fronteira Sul (UFFS)Universidade Federal do Paraná (UFPR)Noda-Nicolau, Nathalia Mayumi [UNESP]de Castro Silva, Mariana [UNESP]Bento, Giovana Fernanda Cosi [UNESP]Ferreira, Jeniffer Sena Baptista [UNESP]Novak, Juliano [UNESP]Morales, Júlia Andrade Pessoa [UNESP]Tronco, Júlia Abbade [UNESP]Bolpetti, Aline Nascimento [UNESP]Pinto, Gabriel Vitor Silva [UNESP]Polettini, JossimaraMarconi, Camila [UNESP]da Silva, Márcia Guimarães [UNESP]2022-04-29T08:37:12Z2022-04-29T08:37:12Z2021-12-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.1371/journal.pone.0260753PLoS ONE, v. 16, n. 12 December, 2021.1932-6203http://hdl.handle.net/11449/23001310.1371/journal.pone.02607532-s2.0-85120713992Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengPLoS ONEinfo:eu-repo/semantics/openAccess2024-09-03T13:15:38Zoai:repositorio.unesp.br:11449/230013Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestrepositoriounesp@unesp.bropendoar:29462024-09-03T13:15:38Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Cervicovaginal levels of human beta defensins during bacterial vaginosis |
title |
Cervicovaginal levels of human beta defensins during bacterial vaginosis |
spellingShingle |
Cervicovaginal levels of human beta defensins during bacterial vaginosis Noda-Nicolau, Nathalia Mayumi [UNESP] |
title_short |
Cervicovaginal levels of human beta defensins during bacterial vaginosis |
title_full |
Cervicovaginal levels of human beta defensins during bacterial vaginosis |
title_fullStr |
Cervicovaginal levels of human beta defensins during bacterial vaginosis |
title_full_unstemmed |
Cervicovaginal levels of human beta defensins during bacterial vaginosis |
title_sort |
Cervicovaginal levels of human beta defensins during bacterial vaginosis |
author |
Noda-Nicolau, Nathalia Mayumi [UNESP] |
author_facet |
Noda-Nicolau, Nathalia Mayumi [UNESP] de Castro Silva, Mariana [UNESP] Bento, Giovana Fernanda Cosi [UNESP] Ferreira, Jeniffer Sena Baptista [UNESP] Novak, Juliano [UNESP] Morales, Júlia Andrade Pessoa [UNESP] Tronco, Júlia Abbade [UNESP] Bolpetti, Aline Nascimento [UNESP] Pinto, Gabriel Vitor Silva [UNESP] Polettini, Jossimara Marconi, Camila [UNESP] da Silva, Márcia Guimarães [UNESP] |
author_role |
author |
author2 |
de Castro Silva, Mariana [UNESP] Bento, Giovana Fernanda Cosi [UNESP] Ferreira, Jeniffer Sena Baptista [UNESP] Novak, Juliano [UNESP] Morales, Júlia Andrade Pessoa [UNESP] Tronco, Júlia Abbade [UNESP] Bolpetti, Aline Nascimento [UNESP] Pinto, Gabriel Vitor Silva [UNESP] Polettini, Jossimara Marconi, Camila [UNESP] da Silva, Márcia Guimarães [UNESP] |
author2_role |
author author author author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (UNESP) Universidade Federal da Fronteira Sul (UFFS) Universidade Federal do Paraná (UFPR) |
dc.contributor.author.fl_str_mv |
Noda-Nicolau, Nathalia Mayumi [UNESP] de Castro Silva, Mariana [UNESP] Bento, Giovana Fernanda Cosi [UNESP] Ferreira, Jeniffer Sena Baptista [UNESP] Novak, Juliano [UNESP] Morales, Júlia Andrade Pessoa [UNESP] Tronco, Júlia Abbade [UNESP] Bolpetti, Aline Nascimento [UNESP] Pinto, Gabriel Vitor Silva [UNESP] Polettini, Jossimara Marconi, Camila [UNESP] da Silva, Márcia Guimarães [UNESP] |
description |
Aims To compare the cervicovaginal levels of human beta defensin (hBD)-1, 2 and 3 of women according to the status of Nugent-defined bacterial vaginosis (BV). Methods A total of 634 women of reproductive age were included in the study. Participants were equally distributed in two groups: according to the classification of vaginal smears according to Nugent criteria in normal (scores 0 to 3) and BV (scores ≥7). Cervicovaginal fluid samples were used for measurements of hBDs1, 2 and 3 levels by enzyme-linked immunosorbent assay (ELISA). Levels of each hBD were compared between the two study groups using Mann-Whitney test, with p-value <0.05 considered as significant. Odds ratio (OR) and 95% confidence interval (95% CI) were calculated for sociodemographic variables and hBD1-3 levels associated with BV a multivariable analysis. Correlation between Nugent score and measured levels of hBDs1-3 were calculated using Spearman’s test. Results Cervicovaginal fluids from women with BV showed lower levels of hBD-1 [median 2,400.00 pg/mL (0–27,800.00); p<0.0001], hBD-2 [5,600.00 pg/mL (0–45,800.00); p<0.0001] and hBD-3 [1,600.00 pg/mL (0–81,700.00); p = 0.012] when compared to optimal microbiota [hBD-1: [median 3,400.00 pg/mL (0–35,600.00), hBD-2: 12,300.00 pg/mL (0–95,300.00) and hBD-3: 3,000.00 pg/mL (0–64,300.00), respectively]. Multivariable analysis showed that lower levels of hBD-1 (OR: 2.05; 95% CI: 1.46–2.87), hBD-2 (OR: 1.85; 95% CI: 1.32–2.60) and hBD-3 (OR: 1.90; 95% CI: 1.37–2.64) were independently associated BV. Significant negative correlations were observed between Nugent scores and cervicovaginal levels of hBD-1 (Spearman’s rho = -0.2118; p = 0.0001) and hBD-2 (*Spearman’s rho = -0.2117; p = 0.0001). Conclusions Bacterial vaginosis is associated with lower cervicovaginal levels of hBDs1-3 in reproductive-aged women. |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021-12-01 2022-04-29T08:37:12Z 2022-04-29T08:37:12Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1371/journal.pone.0260753 PLoS ONE, v. 16, n. 12 December, 2021. 1932-6203 http://hdl.handle.net/11449/230013 10.1371/journal.pone.0260753 2-s2.0-85120713992 |
url |
http://dx.doi.org/10.1371/journal.pone.0260753 http://hdl.handle.net/11449/230013 |
identifier_str_mv |
PLoS ONE, v. 16, n. 12 December, 2021. 1932-6203 10.1371/journal.pone.0260753 2-s2.0-85120713992 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
PLoS ONE |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
repositoriounesp@unesp.br |
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1810021396977287168 |