Polymorphic Sites at the 3 ' Untranslated Region of the HLA-G Gene Are Associated with Differential hla-g Soluble Levels in the Brazilian and French Population
Autor(a) principal: | |
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Data de Publicação: | 2013 |
Outros Autores: | , , , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1371/journal.pone.0071742 http://hdl.handle.net/11449/112289 |
Resumo: | HLA-G molecule has well-recognized tolerogenic properties, and the encoding gene shows lower frequency of polymorphism at the coding region but higher variability at regulatory 5' and 3' untranslated (3' UTR) regions. At least three 3' UTR polymorphic sites have been associated with HLA-G mRNA regulation, including the 14 base pair (14bp) Insertion/Deletion, +3142C-G and +3187A-G. We studied the association of polymorphic sites at 3' UTR (sequencing analysis, encompassing the 14bp Ins-Del/+3003T-C/+3010C-G/+3027C-A/+3035C-T/+3142C-G/+3187A-G/+3196C-G polymorphic sites) with plasma soluble HLA-G levels (sHLA-G, detected by ELISA) in 187 French and 153 Brazilian healthy individuals. Allele and genotype frequencies were closely similar in both populations; however, Brazilians showed a higher HLA-G 3' UTR haplotype diversity. Considering sHLA-G levels in both populations altogether, individuals presenting 14bp Del/Del showed higher levels compared to 14bpIns/Ins genotype (P < 0.05); those presenting +3010C/G showed higher levels compared to the +3010C-C genotype (P<0.05); those presenting +3027C-C showed higher levels than the +3027A-A genotype (P<0.05); and those bearing +3035C-C showed higher levels compared to the +3035C-T (P < 0.01) and +3035T-T (P < 0.05) genotypes. The analyses of 3' UTR haplotypes showed that UTR-1 (DelTGCCCGC) was associated with higher expression of sHLA-G, whereas UTR-5 (InsTCCTGAC) and UTR-7 (InsTCATGAC) with lower expression and other UTRs (UTR-2/3/4/6) exhibited intermediate levels. Since the differential expression of HLA-G may be beneficial or harmful depending on the underlying condition, the identification of individuals genetically programmed to differentially express HLA-G may help on defining novel strategies to control the immune response against the underlying disorder. |
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Polymorphic Sites at the 3 ' Untranslated Region of the HLA-G Gene Are Associated with Differential hla-g Soluble Levels in the Brazilian and French PopulationHLA-G molecule has well-recognized tolerogenic properties, and the encoding gene shows lower frequency of polymorphism at the coding region but higher variability at regulatory 5' and 3' untranslated (3' UTR) regions. At least three 3' UTR polymorphic sites have been associated with HLA-G mRNA regulation, including the 14 base pair (14bp) Insertion/Deletion, +3142C-G and +3187A-G. We studied the association of polymorphic sites at 3' UTR (sequencing analysis, encompassing the 14bp Ins-Del/+3003T-C/+3010C-G/+3027C-A/+3035C-T/+3142C-G/+3187A-G/+3196C-G polymorphic sites) with plasma soluble HLA-G levels (sHLA-G, detected by ELISA) in 187 French and 153 Brazilian healthy individuals. Allele and genotype frequencies were closely similar in both populations; however, Brazilians showed a higher HLA-G 3' UTR haplotype diversity. Considering sHLA-G levels in both populations altogether, individuals presenting 14bp Del/Del showed higher levels compared to 14bpIns/Ins genotype (P < 0.05); those presenting +3010C/G showed higher levels compared to the +3010C-C genotype (P<0.05); those presenting +3027C-C showed higher levels than the +3027A-A genotype (P<0.05); and those bearing +3035C-C showed higher levels compared to the +3035C-T (P < 0.01) and +3035T-T (P < 0.05) genotypes. The analyses of 3' UTR haplotypes showed that UTR-1 (DelTGCCCGC) was associated with higher expression of sHLA-G, whereas UTR-5 (InsTCCTGAC) and UTR-7 (InsTCATGAC) with lower expression and other UTRs (UTR-2/3/4/6) exhibited intermediate levels. Since the differential expression of HLA-G may be beneficial or harmful depending on the underlying condition, the identification of individuals genetically programmed to differentially express HLA-G may help on defining novel strategies to control the immune response against the underlying disorder.Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Commissariat a L'Energie Atomique (CEA), FranceConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)ANR Agence Nationale de la RechercheDeutsche KrebshilfeUniv Sao Paulo, Fac Med Ribeirao Preto, Program Basic & Appl Immunol, Sao Paulo, BrazilHop St Louis, Commissariat Energie Atom & Energies Alternat, Inst Malad Emergentes & Therapies Innovantes, Serv Rech Hematoimmunol, Paris, FranceUniv Paris Diderot, Hop St Louis, Sorbonne Paris Cite, Inst Univ Hematol,Umr E5, Paris, FranceUniv Fed Espirito Santo, Sao Mateus, ES, BrazilUniv Fed Santa Catarina, Program Cellular & Dev Biol, BR-88040900 Florianopolis, SC, BrazilUniv Sao Paulo, Fac Filosofia Ciencias & Letras Ribeirao Preto, Dept Quim, Ribeirao Preto, SP, BrazilUniv State Sao Paulo UNESP, Sch Med Botucatu, Dept Pathol, Botucatu, SP, BrazilUniv Sao Paulo, Sch Med Ribeirao Preto, Dept Med, Div Clin Immunol, Sao Paulo, BrazilUniv Hosp Essen, Inst Transfus Med, Essen, GermanyUniv State Sao Paulo UNESP, Sch Med Botucatu, Dept Pathol, Botucatu, SP, BrazilCAPES: 653/09ANR Agence Nationale de la Recherche2010 PRSP 012 003Deutsche Krebshilfe109816Public Library ScienceUniversidade de São Paulo (USP)Hop St LouisUniv Paris DiderotUniversidade Federal do Espírito Santo (UFES)Universidade Federal de Santa Catarina (UFSC)Universidade Estadual Paulista (Unesp)Univ Hosp EssenMartelli-Palomino, GustavoPancotto, Joao A.Muniz, Yara C.Mendes-Junior, Celso T.Castelli, Erick da Cruz [UNESP]Massaro, Juliana D.Krawice-Radanne, IrenePoras, IsabelleRebmann, VeraCarosella, Edgardo D.Rouas-Freiss, NathalieMoreau, PhilippeDonadi, Eduardo A.2014-12-03T13:10:35Z2014-12-03T13:10:35Z2013-10-25info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article10application/pdfhttp://dx.doi.org/10.1371/journal.pone.0071742Plos One. San Francisco: Public Library Science, v. 8, n. 10, 10 p., 2013.1932-6203http://hdl.handle.net/11449/11228910.1371/journal.pone.0071742WOS:000326155400002WOS000326155400002.pdf09925593181752350000-0003-2142-7196Web of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengPLOS ONE2.7661,164info:eu-repo/semantics/openAccess2024-01-06T06:27:44Zoai:repositorio.unesp.br:11449/112289Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-05-23T19:57:58.035530Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Polymorphic Sites at the 3 ' Untranslated Region of the HLA-G Gene Are Associated with Differential hla-g Soluble Levels in the Brazilian and French Population |
title |
Polymorphic Sites at the 3 ' Untranslated Region of the HLA-G Gene Are Associated with Differential hla-g Soluble Levels in the Brazilian and French Population |
spellingShingle |
Polymorphic Sites at the 3 ' Untranslated Region of the HLA-G Gene Are Associated with Differential hla-g Soluble Levels in the Brazilian and French Population Martelli-Palomino, Gustavo |
title_short |
Polymorphic Sites at the 3 ' Untranslated Region of the HLA-G Gene Are Associated with Differential hla-g Soluble Levels in the Brazilian and French Population |
title_full |
Polymorphic Sites at the 3 ' Untranslated Region of the HLA-G Gene Are Associated with Differential hla-g Soluble Levels in the Brazilian and French Population |
title_fullStr |
Polymorphic Sites at the 3 ' Untranslated Region of the HLA-G Gene Are Associated with Differential hla-g Soluble Levels in the Brazilian and French Population |
title_full_unstemmed |
Polymorphic Sites at the 3 ' Untranslated Region of the HLA-G Gene Are Associated with Differential hla-g Soluble Levels in the Brazilian and French Population |
title_sort |
Polymorphic Sites at the 3 ' Untranslated Region of the HLA-G Gene Are Associated with Differential hla-g Soluble Levels in the Brazilian and French Population |
author |
Martelli-Palomino, Gustavo |
author_facet |
Martelli-Palomino, Gustavo Pancotto, Joao A. Muniz, Yara C. Mendes-Junior, Celso T. Castelli, Erick da Cruz [UNESP] Massaro, Juliana D. Krawice-Radanne, Irene Poras, Isabelle Rebmann, Vera Carosella, Edgardo D. Rouas-Freiss, Nathalie Moreau, Philippe Donadi, Eduardo A. |
author_role |
author |
author2 |
Pancotto, Joao A. Muniz, Yara C. Mendes-Junior, Celso T. Castelli, Erick da Cruz [UNESP] Massaro, Juliana D. Krawice-Radanne, Irene Poras, Isabelle Rebmann, Vera Carosella, Edgardo D. Rouas-Freiss, Nathalie Moreau, Philippe Donadi, Eduardo A. |
author2_role |
author author author author author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade de São Paulo (USP) Hop St Louis Univ Paris Diderot Universidade Federal do Espírito Santo (UFES) Universidade Federal de Santa Catarina (UFSC) Universidade Estadual Paulista (Unesp) Univ Hosp Essen |
dc.contributor.author.fl_str_mv |
Martelli-Palomino, Gustavo Pancotto, Joao A. Muniz, Yara C. Mendes-Junior, Celso T. Castelli, Erick da Cruz [UNESP] Massaro, Juliana D. Krawice-Radanne, Irene Poras, Isabelle Rebmann, Vera Carosella, Edgardo D. Rouas-Freiss, Nathalie Moreau, Philippe Donadi, Eduardo A. |
description |
HLA-G molecule has well-recognized tolerogenic properties, and the encoding gene shows lower frequency of polymorphism at the coding region but higher variability at regulatory 5' and 3' untranslated (3' UTR) regions. At least three 3' UTR polymorphic sites have been associated with HLA-G mRNA regulation, including the 14 base pair (14bp) Insertion/Deletion, +3142C-G and +3187A-G. We studied the association of polymorphic sites at 3' UTR (sequencing analysis, encompassing the 14bp Ins-Del/+3003T-C/+3010C-G/+3027C-A/+3035C-T/+3142C-G/+3187A-G/+3196C-G polymorphic sites) with plasma soluble HLA-G levels (sHLA-G, detected by ELISA) in 187 French and 153 Brazilian healthy individuals. Allele and genotype frequencies were closely similar in both populations; however, Brazilians showed a higher HLA-G 3' UTR haplotype diversity. Considering sHLA-G levels in both populations altogether, individuals presenting 14bp Del/Del showed higher levels compared to 14bpIns/Ins genotype (P < 0.05); those presenting +3010C/G showed higher levels compared to the +3010C-C genotype (P<0.05); those presenting +3027C-C showed higher levels than the +3027A-A genotype (P<0.05); and those bearing +3035C-C showed higher levels compared to the +3035C-T (P < 0.01) and +3035T-T (P < 0.05) genotypes. The analyses of 3' UTR haplotypes showed that UTR-1 (DelTGCCCGC) was associated with higher expression of sHLA-G, whereas UTR-5 (InsTCCTGAC) and UTR-7 (InsTCATGAC) with lower expression and other UTRs (UTR-2/3/4/6) exhibited intermediate levels. Since the differential expression of HLA-G may be beneficial or harmful depending on the underlying condition, the identification of individuals genetically programmed to differentially express HLA-G may help on defining novel strategies to control the immune response against the underlying disorder. |
publishDate |
2013 |
dc.date.none.fl_str_mv |
2013-10-25 2014-12-03T13:10:35Z 2014-12-03T13:10:35Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1371/journal.pone.0071742 Plos One. San Francisco: Public Library Science, v. 8, n. 10, 10 p., 2013. 1932-6203 http://hdl.handle.net/11449/112289 10.1371/journal.pone.0071742 WOS:000326155400002 WOS000326155400002.pdf 0992559318175235 0000-0003-2142-7196 |
url |
http://dx.doi.org/10.1371/journal.pone.0071742 http://hdl.handle.net/11449/112289 |
identifier_str_mv |
Plos One. San Francisco: Public Library Science, v. 8, n. 10, 10 p., 2013. 1932-6203 10.1371/journal.pone.0071742 WOS:000326155400002 WOS000326155400002.pdf 0992559318175235 0000-0003-2142-7196 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
PLOS ONE 2.766 1,164 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
10 application/pdf |
dc.publisher.none.fl_str_mv |
Public Library Science |
publisher.none.fl_str_mv |
Public Library Science |
dc.source.none.fl_str_mv |
Web of Science reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
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1803045641117499392 |