Antifungal activity of plant-derived essential oils on Candida tropicalis planktonic and biofilms cells
Autor(a) principal: | |
---|---|
Data de Publicação: | 2016 |
Outros Autores: | , , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1093/mmy/myw003 http://hdl.handle.net/11449/165220 |
Resumo: | Dental prosthesis supports Candida species growth and may predispose the oral cavity to lesions. C. tropicalis has emerged as a colonizer of prosthesis and has shown resistance to clinically used antifungal agents, which has increased the search for new antifungals. This work describes the effectiveness of fifteen essential oils (EOs) against C. tropicalis. The EOs were obtained by hydrodistillation and were chemically characterized by gas chromatography-mass spectrometry. The antifungal activities of the EOs were evaluated by the microdilution method and showed that Pelargonium graveolens (Geraniaceae) (PG-EO) was the most effective oil. Geraniol and linalool were the major constituents of PG-EO. The 2,3-Bis-(2-Methoxy-4-Nitro-5-Sulfophenyl)-2H-Tetrazolium-5-Carboxanilide (XTT) assay showed that all the clinical C. tropicalis strains formed viable biofilms. Scanning electron microscopy examination of the biofilms revealed a complex architecture with basal layer of yeast cells and an upper layer of filamentous cells. Treatments with PG-EO, linalool, and geraniol significantly reduced the number of viable biofilm cells and inhibited biofilm formation after exposure for 48 h. PG-EO, geraniol, and linalool were not toxic to normal human lung fibroblasts (GM07492A) at the concentrations they were active against C. tropicalis. Together, our results indicated that C. tropicalis is susceptible to treatment with PG-EO, geraniol, and linalool, which could become options to prevent or treat this infection. |
id |
UNSP_9e67f6e986b0e04a7c8542d908bacdbb |
---|---|
oai_identifier_str |
oai:repositorio.unesp.br:11449/165220 |
network_acronym_str |
UNSP |
network_name_str |
Repositório Institucional da UNESP |
repository_id_str |
2946 |
spelling |
Antifungal activity of plant-derived essential oils on Candida tropicalis planktonic and biofilms cellsC. tropicalisessential oilsP. graveolensbiofilmsDental prosthesis supports Candida species growth and may predispose the oral cavity to lesions. C. tropicalis has emerged as a colonizer of prosthesis and has shown resistance to clinically used antifungal agents, which has increased the search for new antifungals. This work describes the effectiveness of fifteen essential oils (EOs) against C. tropicalis. The EOs were obtained by hydrodistillation and were chemically characterized by gas chromatography-mass spectrometry. The antifungal activities of the EOs were evaluated by the microdilution method and showed that Pelargonium graveolens (Geraniaceae) (PG-EO) was the most effective oil. Geraniol and linalool were the major constituents of PG-EO. The 2,3-Bis-(2-Methoxy-4-Nitro-5-Sulfophenyl)-2H-Tetrazolium-5-Carboxanilide (XTT) assay showed that all the clinical C. tropicalis strains formed viable biofilms. Scanning electron microscopy examination of the biofilms revealed a complex architecture with basal layer of yeast cells and an upper layer of filamentous cells. Treatments with PG-EO, linalool, and geraniol significantly reduced the number of viable biofilm cells and inhibited biofilm formation after exposure for 48 h. PG-EO, geraniol, and linalool were not toxic to normal human lung fibroblasts (GM07492A) at the concentrations they were active against C. tropicalis. Together, our results indicated that C. tropicalis is susceptible to treatment with PG-EO, geraniol, and linalool, which could become options to prevent or treat this infection.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Univ Franca, Lab Pesquisa Microbiol Aplicada, Franca, SP, BrazilUniv Sao Paulo, Fac Filosofia Ciencias & Letras Ribeirao Preto, Sao Paulo, BrazilUniv Fed Mato Grosso, Inst Ciencias Exatas & Terra, Pontal Do Araguaia, MT, BrazilUniv Estadual Paulista, Fac Ciencias Farmaceut, Sao Paulo, BrazilUniv Estadual Paulista, Fac Ciencias Farmaceut, Sao Paulo, BrazilFAPESP: 2011/12734-3FAPESP: 2007/54241-8Oxford Univ PressUniv FrancaUniversidade de São Paulo (USP)Universidade Federal de Mato Grosso do Sul (UFMS)Universidade Estadual Paulista (Unesp)Cury Souza, Caio MarceloPereira Junior, Silvio AlvesMoraes, Thais da SilvaDamasceno, Jaqueline LopesMendes, Suzana AmorimDias, Herbert JuniorStefani, RicardoTavares, Denise CrispimGomes Martins, Carlos HenriqueMiller Crotti, Antonio EduardoSoares Mendes-Giannini, Maria Jose [UNESP]Pires, Regina Helena [UNESP]2018-11-27T16:55:46Z2018-11-27T16:55:46Z2016-07-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article515-523application/pdfhttp://dx.doi.org/10.1093/mmy/myw003Medical Mycology. Oxford: Oxford Univ Press, v. 54, n. 5, p. 515-523, 2016.1369-3786http://hdl.handle.net/11449/16522010.1093/mmy/myw003WOS:000378865500007WOS000378865500007.pdfWeb of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengMedical Mycology0,973info:eu-repo/semantics/openAccess2024-06-21T15:18:57Zoai:repositorio.unesp.br:11449/165220Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T18:56:47.639583Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Antifungal activity of plant-derived essential oils on Candida tropicalis planktonic and biofilms cells |
title |
Antifungal activity of plant-derived essential oils on Candida tropicalis planktonic and biofilms cells |
spellingShingle |
Antifungal activity of plant-derived essential oils on Candida tropicalis planktonic and biofilms cells Cury Souza, Caio Marcelo C. tropicalis essential oils P. graveolens biofilms |
title_short |
Antifungal activity of plant-derived essential oils on Candida tropicalis planktonic and biofilms cells |
title_full |
Antifungal activity of plant-derived essential oils on Candida tropicalis planktonic and biofilms cells |
title_fullStr |
Antifungal activity of plant-derived essential oils on Candida tropicalis planktonic and biofilms cells |
title_full_unstemmed |
Antifungal activity of plant-derived essential oils on Candida tropicalis planktonic and biofilms cells |
title_sort |
Antifungal activity of plant-derived essential oils on Candida tropicalis planktonic and biofilms cells |
author |
Cury Souza, Caio Marcelo |
author_facet |
Cury Souza, Caio Marcelo Pereira Junior, Silvio Alves Moraes, Thais da Silva Damasceno, Jaqueline Lopes Mendes, Suzana Amorim Dias, Herbert Junior Stefani, Ricardo Tavares, Denise Crispim Gomes Martins, Carlos Henrique Miller Crotti, Antonio Eduardo Soares Mendes-Giannini, Maria Jose [UNESP] Pires, Regina Helena [UNESP] |
author_role |
author |
author2 |
Pereira Junior, Silvio Alves Moraes, Thais da Silva Damasceno, Jaqueline Lopes Mendes, Suzana Amorim Dias, Herbert Junior Stefani, Ricardo Tavares, Denise Crispim Gomes Martins, Carlos Henrique Miller Crotti, Antonio Eduardo Soares Mendes-Giannini, Maria Jose [UNESP] Pires, Regina Helena [UNESP] |
author2_role |
author author author author author author author author author author author |
dc.contributor.none.fl_str_mv |
Univ Franca Universidade de São Paulo (USP) Universidade Federal de Mato Grosso do Sul (UFMS) Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Cury Souza, Caio Marcelo Pereira Junior, Silvio Alves Moraes, Thais da Silva Damasceno, Jaqueline Lopes Mendes, Suzana Amorim Dias, Herbert Junior Stefani, Ricardo Tavares, Denise Crispim Gomes Martins, Carlos Henrique Miller Crotti, Antonio Eduardo Soares Mendes-Giannini, Maria Jose [UNESP] Pires, Regina Helena [UNESP] |
dc.subject.por.fl_str_mv |
C. tropicalis essential oils P. graveolens biofilms |
topic |
C. tropicalis essential oils P. graveolens biofilms |
description |
Dental prosthesis supports Candida species growth and may predispose the oral cavity to lesions. C. tropicalis has emerged as a colonizer of prosthesis and has shown resistance to clinically used antifungal agents, which has increased the search for new antifungals. This work describes the effectiveness of fifteen essential oils (EOs) against C. tropicalis. The EOs were obtained by hydrodistillation and were chemically characterized by gas chromatography-mass spectrometry. The antifungal activities of the EOs were evaluated by the microdilution method and showed that Pelargonium graveolens (Geraniaceae) (PG-EO) was the most effective oil. Geraniol and linalool were the major constituents of PG-EO. The 2,3-Bis-(2-Methoxy-4-Nitro-5-Sulfophenyl)-2H-Tetrazolium-5-Carboxanilide (XTT) assay showed that all the clinical C. tropicalis strains formed viable biofilms. Scanning electron microscopy examination of the biofilms revealed a complex architecture with basal layer of yeast cells and an upper layer of filamentous cells. Treatments with PG-EO, linalool, and geraniol significantly reduced the number of viable biofilm cells and inhibited biofilm formation after exposure for 48 h. PG-EO, geraniol, and linalool were not toxic to normal human lung fibroblasts (GM07492A) at the concentrations they were active against C. tropicalis. Together, our results indicated that C. tropicalis is susceptible to treatment with PG-EO, geraniol, and linalool, which could become options to prevent or treat this infection. |
publishDate |
2016 |
dc.date.none.fl_str_mv |
2016-07-01 2018-11-27T16:55:46Z 2018-11-27T16:55:46Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1093/mmy/myw003 Medical Mycology. Oxford: Oxford Univ Press, v. 54, n. 5, p. 515-523, 2016. 1369-3786 http://hdl.handle.net/11449/165220 10.1093/mmy/myw003 WOS:000378865500007 WOS000378865500007.pdf |
url |
http://dx.doi.org/10.1093/mmy/myw003 http://hdl.handle.net/11449/165220 |
identifier_str_mv |
Medical Mycology. Oxford: Oxford Univ Press, v. 54, n. 5, p. 515-523, 2016. 1369-3786 10.1093/mmy/myw003 WOS:000378865500007 WOS000378865500007.pdf |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Medical Mycology 0,973 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
515-523 application/pdf |
dc.publisher.none.fl_str_mv |
Oxford Univ Press |
publisher.none.fl_str_mv |
Oxford Univ Press |
dc.source.none.fl_str_mv |
Web of Science reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808129001831530496 |