Ação de nanopartículas de prata sobre Candida albicans e Candida glabrata: análise da mínima concentração inibitória

• Autor(a) principal: Vasconcellos, Ligia Kerche [UNESP]
• Data de Publicação: 2012
• Tipo de documento: Trabalho de conclusão de curso
• Idioma: por
• Título da fonte: Repositório Institucional da UNESP
• Texto Completo: http://hdl.handle.net/11449/149459; http://www.athena.biblioteca.unesp.br/exlibris/bd/capelo/2017-01-27/000855794.pdf
• topic: Nanotecnologia; Candida; Estomatite sob prótese; Candida albicans; Candida glabrata; Nanotechnology

Silver is a metal known for its broad-spectrum antimicrobial activity against Gram-positive and Gram-negative bacteria, fungi, protozoa and certain viruses, including antibiotic-resistant strains. Antimicrobial activity of Ag nanoparticles can be presented more effective because they are extremely small particles and exhibit a larger surface-to-volume ratio. The incorporation of these nanoparticles to dental polymers could benefit denture wearers by preventing pathologies such as denture stomatitis. The aim of this study was to evaluate the minimum inhibitory concentration (MIC) of different types of silver nanoparticles on Candida albicans and Candida glabrata biofilms. Silver nanoparticles were synthesized through the reduction of silver ions of silver nitrate by sodium citrate. The variation factors were size of silver nanoparticles (5, 10 e 60 nm), concentration of silver in the colloidal solution (107,9 μl/mL; 2 x 107,9 μl/mL e 5 x 107,9 μl/mL) and type of silver nanoparticles (colloidal ammonia stabilized and polyvinylpyrrolidone (PVP) coated). Sterile deionized water and nistatina were used as positive and negative control, respectively. MIC was evaluated through inhibition zone with filter paper and pour plate techniques. Fungicide or fungiostatic effects of silver nanoparticles were analyzed though sowing material collected from the inhibition zone. Results obtained from both techniques showed that silver nanoparticles ammonia stabilized and PVP coated, with 5, 10 and 60 nm and in three different concentrations inhibited Candida glabrata and Candida albicans similarly, suggesting that silver nanoparticles may eventually be added to denture base polymers and assist in controlling diseases such as denture stomatitis