Ethanol biosynthesis by fast hydrolysis of cassava bagasse using fungal amylases produced in optimized conditions
Autor(a) principal: | |
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Data de Publicação: | 2018 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1016/j.indcrop.2017.12.004 http://hdl.handle.net/11449/163878 |
Resumo: | The search for a renewable platform to produce high-value biochemicals and energy that are environmentally correct has been a current concern. A fast and inexpensive bioprocess for amylase production, able to hydrolyze complex residues in fermentable sugars to be used for ethanol production was developed. High titer amylase from Rhizopus oligosporus in solid state fermentation (SSF) was obtained by optimizing the medium supplementation using agro-industrial waste as substrate. Statistical experimental design and partial least square (PLS) regression were used to establish a relation between added chemical compounds and enzyme production, showing that urea was the most important nutrient. Crude amylase extract had competitive performance features giving higher productivities in starch hydrolysis than a commercial glucoamylase. The amylase produced was applied in the proportion of 15 U/g dry cassava bagasse to obtain cassava bagasse hydrolysate (CBH). More than 42% conversion in reducing sugars was achieved with an efficient 10 h single-step hydrolysis at 55 degrees C in a bioreactor. The concentrated CBH was subsequently used in fed batch process producing 89.2% ethanol yield. Furthermore, comparing just the cost of the raw materials sugarcane and CHB, the latter demonstrated to be a lower-cost feedstock for ethanol fermentation. |
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Ethanol biosynthesis by fast hydrolysis of cassava bagasse using fungal amylases produced in optimized conditionsAmylaseRhizopus oligosporusSolid state fermentationCassava bagasse hydrolysateGlucoseEthanol productionThe search for a renewable platform to produce high-value biochemicals and energy that are environmentally correct has been a current concern. A fast and inexpensive bioprocess for amylase production, able to hydrolyze complex residues in fermentable sugars to be used for ethanol production was developed. High titer amylase from Rhizopus oligosporus in solid state fermentation (SSF) was obtained by optimizing the medium supplementation using agro-industrial waste as substrate. Statistical experimental design and partial least square (PLS) regression were used to establish a relation between added chemical compounds and enzyme production, showing that urea was the most important nutrient. Crude amylase extract had competitive performance features giving higher productivities in starch hydrolysis than a commercial glucoamylase. The amylase produced was applied in the proportion of 15 U/g dry cassava bagasse to obtain cassava bagasse hydrolysate (CBH). More than 42% conversion in reducing sugars was achieved with an efficient 10 h single-step hydrolysis at 55 degrees C in a bioreactor. The concentrated CBH was subsequently used in fed batch process producing 89.2% ethanol yield. Furthermore, comparing just the cost of the raw materials sugarcane and CHB, the latter demonstrated to be a lower-cost feedstock for ethanol fermentation.Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Univ Estadual Paulista, Dept Biotecnol, Inst Pesquisa Bioenergia, Campus Assis,Ave Dom Antonio 2100, BR-19806900 Assis, SP, BrazilUniv Estadual Paulista, Dept Ciencias Biol, Grp Engn Bioproc, Campus Assis,Ave Dom Antonio 2100, BR-19806900 Assis, SP, BrazilUniv Estadual Paulista, Dept Biotecnol, Inst Pesquisa Bioenergia, Campus Assis,Ave Dom Antonio 2100, BR-19806900 Assis, SP, BrazilUniv Estadual Paulista, Dept Ciencias Biol, Grp Engn Bioproc, Campus Assis,Ave Dom Antonio 2100, BR-19806900 Assis, SP, BrazilCNPq: 141472/2015-4FAPESP: 2012/16651-8FAPESP: 2014/24188-1Elsevier B.V.Universidade Estadual Paulista (Unesp)Escaramboni, Bruna [UNESP]Fernandez Nunez, Eutimio Gustavo [UNESP]Azevedo Carvalho, Ana Flavia [UNESP]Oliva Neto, Pedro de [UNESP]2018-11-26T17:48:16Z2018-11-26T17:48:16Z2018-02-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article368-377application/pdfhttp://dx.doi.org/10.1016/j.indcrop.2017.12.004Industrial Crops And Products. Amsterdam: Elsevier Science Bv, v. 112, p. 368-377, 2018.0926-6690http://hdl.handle.net/11449/16387810.1016/j.indcrop.2017.12.004WOS:000425561900045WOS000425561900045.pdf46389522635027440000-0001-9378-9036Web of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengIndustrial Crops And Products1,091info:eu-repo/semantics/openAccess2024-01-14T06:19:22Zoai:repositorio.unesp.br:11449/163878Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-01-14T06:19:22Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Ethanol biosynthesis by fast hydrolysis of cassava bagasse using fungal amylases produced in optimized conditions |
title |
Ethanol biosynthesis by fast hydrolysis of cassava bagasse using fungal amylases produced in optimized conditions |
spellingShingle |
Ethanol biosynthesis by fast hydrolysis of cassava bagasse using fungal amylases produced in optimized conditions Escaramboni, Bruna [UNESP] Amylase Rhizopus oligosporus Solid state fermentation Cassava bagasse hydrolysate Glucose Ethanol production |
title_short |
Ethanol biosynthesis by fast hydrolysis of cassava bagasse using fungal amylases produced in optimized conditions |
title_full |
Ethanol biosynthesis by fast hydrolysis of cassava bagasse using fungal amylases produced in optimized conditions |
title_fullStr |
Ethanol biosynthesis by fast hydrolysis of cassava bagasse using fungal amylases produced in optimized conditions |
title_full_unstemmed |
Ethanol biosynthesis by fast hydrolysis of cassava bagasse using fungal amylases produced in optimized conditions |
title_sort |
Ethanol biosynthesis by fast hydrolysis of cassava bagasse using fungal amylases produced in optimized conditions |
author |
Escaramboni, Bruna [UNESP] |
author_facet |
Escaramboni, Bruna [UNESP] Fernandez Nunez, Eutimio Gustavo [UNESP] Azevedo Carvalho, Ana Flavia [UNESP] Oliva Neto, Pedro de [UNESP] |
author_role |
author |
author2 |
Fernandez Nunez, Eutimio Gustavo [UNESP] Azevedo Carvalho, Ana Flavia [UNESP] Oliva Neto, Pedro de [UNESP] |
author2_role |
author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Escaramboni, Bruna [UNESP] Fernandez Nunez, Eutimio Gustavo [UNESP] Azevedo Carvalho, Ana Flavia [UNESP] Oliva Neto, Pedro de [UNESP] |
dc.subject.por.fl_str_mv |
Amylase Rhizopus oligosporus Solid state fermentation Cassava bagasse hydrolysate Glucose Ethanol production |
topic |
Amylase Rhizopus oligosporus Solid state fermentation Cassava bagasse hydrolysate Glucose Ethanol production |
description |
The search for a renewable platform to produce high-value biochemicals and energy that are environmentally correct has been a current concern. A fast and inexpensive bioprocess for amylase production, able to hydrolyze complex residues in fermentable sugars to be used for ethanol production was developed. High titer amylase from Rhizopus oligosporus in solid state fermentation (SSF) was obtained by optimizing the medium supplementation using agro-industrial waste as substrate. Statistical experimental design and partial least square (PLS) regression were used to establish a relation between added chemical compounds and enzyme production, showing that urea was the most important nutrient. Crude amylase extract had competitive performance features giving higher productivities in starch hydrolysis than a commercial glucoamylase. The amylase produced was applied in the proportion of 15 U/g dry cassava bagasse to obtain cassava bagasse hydrolysate (CBH). More than 42% conversion in reducing sugars was achieved with an efficient 10 h single-step hydrolysis at 55 degrees C in a bioreactor. The concentrated CBH was subsequently used in fed batch process producing 89.2% ethanol yield. Furthermore, comparing just the cost of the raw materials sugarcane and CHB, the latter demonstrated to be a lower-cost feedstock for ethanol fermentation. |
publishDate |
2018 |
dc.date.none.fl_str_mv |
2018-11-26T17:48:16Z 2018-11-26T17:48:16Z 2018-02-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1016/j.indcrop.2017.12.004 Industrial Crops And Products. Amsterdam: Elsevier Science Bv, v. 112, p. 368-377, 2018. 0926-6690 http://hdl.handle.net/11449/163878 10.1016/j.indcrop.2017.12.004 WOS:000425561900045 WOS000425561900045.pdf 4638952263502744 0000-0001-9378-9036 |
url |
http://dx.doi.org/10.1016/j.indcrop.2017.12.004 http://hdl.handle.net/11449/163878 |
identifier_str_mv |
Industrial Crops And Products. Amsterdam: Elsevier Science Bv, v. 112, p. 368-377, 2018. 0926-6690 10.1016/j.indcrop.2017.12.004 WOS:000425561900045 WOS000425561900045.pdf 4638952263502744 0000-0001-9378-9036 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Industrial Crops And Products 1,091 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
368-377 application/pdf |
dc.publisher.none.fl_str_mv |
Elsevier B.V. |
publisher.none.fl_str_mv |
Elsevier B.V. |
dc.source.none.fl_str_mv |
Web of Science reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1799965611732762624 |