A preliminary differential proteomic analysis of the periplasmic fraction in Acidithiobacillus ferrooxidans planktonic and attached cells exposed to chalcopyrite
Autor(a) principal: | |
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Data de Publicação: | 2009 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo de conferência |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.4028/www.scientific.net/AMR.71-73.179 http://hdl.handle.net/11449/71497 |
Resumo: | The Acidithiobacillus ferrooxidans periplasmic space is known to have proteins involved in the respiratory chains. There are no reports about the expression of the periplasmic proteins in A. ferrooxidans cells attached to chalcopyrite. In this preliminary work, it was compared the periplasmic protein profiles of A. ferrooxidans planktonic and attached cells after exposure to chalcopyrite for 2 hours. The bacterial response to chalcopyrite was investigated by a proteomic approach (two- dimensional gel electrophoresis and mass spectrometry). Four proteins differentially expressed between planktonic and attached cells after exposure to chalcopyrite were identified. Two of these proteins, repressed in chalcopyrite- attached cells, were both identified as superoxide dismutase, whereas the single strand binding protein (SSB) and the PspA/IM30 protein were induced. These results showed that A. ferrooxidans chalcopyrite- attached and planktonic cells show differential expression of the periplasmic proteins and that a proteomic approach can provide a valuable tool to detect proteins related to the A. ferrooxidans response to attachment to the mineral substrates. © (2009) Trans Tech Publications. |
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A preliminary differential proteomic analysis of the periplasmic fraction in Acidithiobacillus ferrooxidans planktonic and attached cells exposed to chalcopyriteAcidithiobacillus ferrooxidansBiofilmChalcopyriteDifferential proteomic analysisPeriplasmic proteinsTwo-dimensional gel electrophoresisDifferential expressionsFerrooxidansMineral substratesPeriplasmic spacePlanktonic cellsProteomic analysisProteomic approachesRespiratory chainsSingle-strand binding proteinSuper oxide dismutaseBiofilmsCopper compoundsElectrophoresisGelsMass spectrometryMetal recoveryOxygenTwo dimensionalProteinsThe Acidithiobacillus ferrooxidans periplasmic space is known to have proteins involved in the respiratory chains. There are no reports about the expression of the periplasmic proteins in A. ferrooxidans cells attached to chalcopyrite. In this preliminary work, it was compared the periplasmic protein profiles of A. ferrooxidans planktonic and attached cells after exposure to chalcopyrite for 2 hours. The bacterial response to chalcopyrite was investigated by a proteomic approach (two- dimensional gel electrophoresis and mass spectrometry). Four proteins differentially expressed between planktonic and attached cells after exposure to chalcopyrite were identified. Two of these proteins, repressed in chalcopyrite- attached cells, were both identified as superoxide dismutase, whereas the single strand binding protein (SSB) and the PspA/IM30 protein were induced. These results showed that A. ferrooxidans chalcopyrite- attached and planktonic cells show differential expression of the periplasmic proteins and that a proteomic approach can provide a valuable tool to detect proteins related to the A. ferrooxidans response to attachment to the mineral substrates. © (2009) Trans Tech Publications.Institute of Chemistry-São Paulo State University, Araraquara-SPProteomics Platform-Barcelona Science Park, BarcelonaCenter of Molecular Biology and Genetic Engineering Campinas State University, Campinas-SPInstitute of Chemistry-São Paulo State University, Araraquara-SPUniversidade Estadual Paulista (Unesp)Proteomics Platform-Barcelona Science ParkUniversidade Estadual de Campinas (UNICAMP)Felício, A. P. [UNESP]De Oliveira, E.Odena, M. A.Garcia, O. [UNESP]Bertolini, M. C. [UNESP]Ferraz, L. F COttoboni, L. M MNovo, M. T M2014-05-27T11:24:35Z2014-05-27T11:24:35Z2009-12-28info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObject179-182http://dx.doi.org/10.4028/www.scientific.net/AMR.71-73.179Advanced Materials Research, v. 71-73, p. 179-182.1022-6680http://hdl.handle.net/11449/7149710.4028/www.scientific.net/AMR.71-73.179WOS:0002735416000382-s2.0-72449206562Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengAdvanced Materials Research0,121info:eu-repo/semantics/openAccess2021-10-23T21:41:35Zoai:repositorio.unesp.br:11449/71497Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T16:20:21.304370Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
A preliminary differential proteomic analysis of the periplasmic fraction in Acidithiobacillus ferrooxidans planktonic and attached cells exposed to chalcopyrite |
title |
A preliminary differential proteomic analysis of the periplasmic fraction in Acidithiobacillus ferrooxidans planktonic and attached cells exposed to chalcopyrite |
spellingShingle |
A preliminary differential proteomic analysis of the periplasmic fraction in Acidithiobacillus ferrooxidans planktonic and attached cells exposed to chalcopyrite Felício, A. P. [UNESP] Acidithiobacillus ferrooxidans Biofilm Chalcopyrite Differential proteomic analysis Periplasmic proteins Two-dimensional gel electrophoresis Differential expressions Ferrooxidans Mineral substrates Periplasmic space Planktonic cells Proteomic analysis Proteomic approaches Respiratory chains Single-strand binding protein Super oxide dismutase Biofilms Copper compounds Electrophoresis Gels Mass spectrometry Metal recovery Oxygen Two dimensional Proteins |
title_short |
A preliminary differential proteomic analysis of the periplasmic fraction in Acidithiobacillus ferrooxidans planktonic and attached cells exposed to chalcopyrite |
title_full |
A preliminary differential proteomic analysis of the periplasmic fraction in Acidithiobacillus ferrooxidans planktonic and attached cells exposed to chalcopyrite |
title_fullStr |
A preliminary differential proteomic analysis of the periplasmic fraction in Acidithiobacillus ferrooxidans planktonic and attached cells exposed to chalcopyrite |
title_full_unstemmed |
A preliminary differential proteomic analysis of the periplasmic fraction in Acidithiobacillus ferrooxidans planktonic and attached cells exposed to chalcopyrite |
title_sort |
A preliminary differential proteomic analysis of the periplasmic fraction in Acidithiobacillus ferrooxidans planktonic and attached cells exposed to chalcopyrite |
author |
Felício, A. P. [UNESP] |
author_facet |
Felício, A. P. [UNESP] De Oliveira, E. Odena, M. A. Garcia, O. [UNESP] Bertolini, M. C. [UNESP] Ferraz, L. F C Ottoboni, L. M M Novo, M. T M |
author_role |
author |
author2 |
De Oliveira, E. Odena, M. A. Garcia, O. [UNESP] Bertolini, M. C. [UNESP] Ferraz, L. F C Ottoboni, L. M M Novo, M. T M |
author2_role |
author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) Proteomics Platform-Barcelona Science Park Universidade Estadual de Campinas (UNICAMP) |
dc.contributor.author.fl_str_mv |
Felício, A. P. [UNESP] De Oliveira, E. Odena, M. A. Garcia, O. [UNESP] Bertolini, M. C. [UNESP] Ferraz, L. F C Ottoboni, L. M M Novo, M. T M |
dc.subject.por.fl_str_mv |
Acidithiobacillus ferrooxidans Biofilm Chalcopyrite Differential proteomic analysis Periplasmic proteins Two-dimensional gel electrophoresis Differential expressions Ferrooxidans Mineral substrates Periplasmic space Planktonic cells Proteomic analysis Proteomic approaches Respiratory chains Single-strand binding protein Super oxide dismutase Biofilms Copper compounds Electrophoresis Gels Mass spectrometry Metal recovery Oxygen Two dimensional Proteins |
topic |
Acidithiobacillus ferrooxidans Biofilm Chalcopyrite Differential proteomic analysis Periplasmic proteins Two-dimensional gel electrophoresis Differential expressions Ferrooxidans Mineral substrates Periplasmic space Planktonic cells Proteomic analysis Proteomic approaches Respiratory chains Single-strand binding protein Super oxide dismutase Biofilms Copper compounds Electrophoresis Gels Mass spectrometry Metal recovery Oxygen Two dimensional Proteins |
description |
The Acidithiobacillus ferrooxidans periplasmic space is known to have proteins involved in the respiratory chains. There are no reports about the expression of the periplasmic proteins in A. ferrooxidans cells attached to chalcopyrite. In this preliminary work, it was compared the periplasmic protein profiles of A. ferrooxidans planktonic and attached cells after exposure to chalcopyrite for 2 hours. The bacterial response to chalcopyrite was investigated by a proteomic approach (two- dimensional gel electrophoresis and mass spectrometry). Four proteins differentially expressed between planktonic and attached cells after exposure to chalcopyrite were identified. Two of these proteins, repressed in chalcopyrite- attached cells, were both identified as superoxide dismutase, whereas the single strand binding protein (SSB) and the PspA/IM30 protein were induced. These results showed that A. ferrooxidans chalcopyrite- attached and planktonic cells show differential expression of the periplasmic proteins and that a proteomic approach can provide a valuable tool to detect proteins related to the A. ferrooxidans response to attachment to the mineral substrates. © (2009) Trans Tech Publications. |
publishDate |
2009 |
dc.date.none.fl_str_mv |
2009-12-28 2014-05-27T11:24:35Z 2014-05-27T11:24:35Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/conferenceObject |
format |
conferenceObject |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.4028/www.scientific.net/AMR.71-73.179 Advanced Materials Research, v. 71-73, p. 179-182. 1022-6680 http://hdl.handle.net/11449/71497 10.4028/www.scientific.net/AMR.71-73.179 WOS:000273541600038 2-s2.0-72449206562 |
url |
http://dx.doi.org/10.4028/www.scientific.net/AMR.71-73.179 http://hdl.handle.net/11449/71497 |
identifier_str_mv |
Advanced Materials Research, v. 71-73, p. 179-182. 1022-6680 10.4028/www.scientific.net/AMR.71-73.179 WOS:000273541600038 2-s2.0-72449206562 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Advanced Materials Research 0,121 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
179-182 |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808128635408744448 |