Implante dérmico acelular sintético no subcutâneo e na superfície da pele de cobaias
Autor(a) principal: | |
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Data de Publicação: | 2015 |
Tipo de documento: | Tese |
Idioma: | por |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://hdl.handle.net/11449/142996 http://www.athena.biblioteca.unesp.br/exlibris/bd/cathedra/08-07-2016/000865259.pdf |
Resumo: | The Nanoskin® is a bacterial cellulose film produced by the bacteria Acetobacter xylinum by means of a biotechnological process. It is composed by a network of nanofibrils whose structure creates a large surface area, which allows the retention of a large amount of water and significant changes in its shape without losing its structural characteristics. Purpose: aiming to add new possibilities for the reconstruction of the eyelid, in its superficial or deep lamellae, this study was developed in order to assess whether the Nanoskin® film could be an option. Method: 40 male guinea pig were used, that received Nanoskin® fragments in the dorsal region, in two studies, one directed to the use of Nanoskin® in deep tissue when the biomaterial was placed subcutaneously and another in which the Nanoskin® was placed on skin surface. In both studies we used two types of Nanoskin: group 1 (G1), which was used Nanoskin® film (2x2 cm) without gelatin coating on the subcutaneous or on the skin surface, and Group 2 (G2), which received Nanoskin® implant (2X2 cm) with gelatin coating on the subcutaneous and on the skin surface. The control group was obtained with full-thickness skin graft placement on the size of 2X2 cm Nanoskin® adjacent to the implant in all animals in G1 and G2 experiment that studied the biomaterial on the surface of the skin. Five animals from each group were euthanized at four experimental times: 7 days (M1), 30 days (M2), 90 days (M3) and 180 days (M4). Morphometric assessments of the implant and the histological slides were held, histological and ultrastructural examination. Results: the Nanoskin® when implanted subcutaneously was not found in one animal from M3 and in five animals from M4. In moments M3, M4 and M5, there was separation between their lamellae . There was a significant inflammation at the beginning of the experiment which reduced the following times, and formation of a pseudocapsule around the ... |
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Implante dérmico acelular sintético no subcutâneo e na superfície da pele de cobaiasPeleImplantes artificiaisCobaiasCelulose bacterianaCelulose - BiodegradaçãoCellulose - BiodegradationThe Nanoskin® is a bacterial cellulose film produced by the bacteria Acetobacter xylinum by means of a biotechnological process. It is composed by a network of nanofibrils whose structure creates a large surface area, which allows the retention of a large amount of water and significant changes in its shape without losing its structural characteristics. Purpose: aiming to add new possibilities for the reconstruction of the eyelid, in its superficial or deep lamellae, this study was developed in order to assess whether the Nanoskin® film could be an option. Method: 40 male guinea pig were used, that received Nanoskin® fragments in the dorsal region, in two studies, one directed to the use of Nanoskin® in deep tissue when the biomaterial was placed subcutaneously and another in which the Nanoskin® was placed on skin surface. In both studies we used two types of Nanoskin: group 1 (G1), which was used Nanoskin® film (2x2 cm) without gelatin coating on the subcutaneous or on the skin surface, and Group 2 (G2), which received Nanoskin® implant (2X2 cm) with gelatin coating on the subcutaneous and on the skin surface. The control group was obtained with full-thickness skin graft placement on the size of 2X2 cm Nanoskin® adjacent to the implant in all animals in G1 and G2 experiment that studied the biomaterial on the surface of the skin. Five animals from each group were euthanized at four experimental times: 7 days (M1), 30 days (M2), 90 days (M3) and 180 days (M4). Morphometric assessments of the implant and the histological slides were held, histological and ultrastructural examination. Results: the Nanoskin® when implanted subcutaneously was not found in one animal from M3 and in five animals from M4. In moments M3, M4 and M5, there was separation between their lamellae . There was a significant inflammation at the beginning of the experiment which reduced the following times, and formation of a pseudocapsule around the ...A Nanoskin® é uma película de celulose bacteriana produzida pela bactéria Acetobacter xylinum,por meio de um processo biotecnológico. É composta por uma rede de nanofibrilas cuja estrutura cria uma extensa superfície, a qual permite a retenção de grande quantidade de água e importantes modificações em seu formato, sem perder suas características estruturais. Objetivo: visando acrescentar novas possibilidades para a reconstrução da pálpebra, em seus folhetos profundo ou superficial, o presente estudo foi desenvolvido com o objetivo de avaliar se a película de Nanoskin® poderia ser uma opção. Método: foram utilizadas 40 cobaias, do sexo masculino, que receberam fragmentos de Nanoskin® na região dorsal, em dois estudos, um direcionado para a utilização da Nanoskin nos tecidos profundos, quando o biomaterial foi colocado no subcutâneo e outro no qual a Nanoskin foi colocada na superfície da pele. Em ambos os estudos foram utilizados dois tipos de Nanoskin: grupo 1 (G1), no qual foi utilizada película de Nanoskin® (2X2 cm) sem recobrimento de gelatina no subcutâneo ou na superfície da pele e o grupo 2 (G2), que recebeu implante de Nanoskin® (2X2 cm) com revestimento de gelatina no subcutâneo e na superfície da pele. O grupo controle foi obtido com colocação de enxerto de pele de espessura total no tamanho de 2X2 cm, contíguo ao implante de Nanoskin®, em todos os animais de G1 e de G2 do experimento que estudou o biomaterial na superfície da pele. Cinco animais de cada grupo foram eutanasiados em quatro momentos experimentais: 7 dias (M1), 30 dias (M2), 90 dias (M3) e 180 dias (M4). Foram realizadas avaliações morfométricas do implante das lâminas histológicas, exame histológico e exame ultraestrutural. Resultados: a Nanoskin® quando implantada no subcutâneo não foi encontrada em um animal de M3 e em cinco animais de M4. Nos momentos M3, M4 e M5, houve separação entre as suas lamelas. Houve...Universidade Estadual Paulista (Unesp)Schellini, Silvana Artioli [UNESP]Universidade Estadual Paulista (Unesp)Natsuaki, Kryscia Leiko [UNESP]2016-08-12T18:48:46Z2016-08-12T18:48:46Z2015-07-16info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/doctoralThesis94 f.application/pdfNATSUAKI, Kryscia Leiko. Implante dérmico acelular sintético no subcutâneo e na superfície da pele de cobaias. 2015. 94 f. Tese (doutorado) - Universidade Estadual Paulista Júlio de Mesquita Filho, Faculdade de Medicina de Botucatu, 2015.http://hdl.handle.net/11449/142996000865259http://www.athena.biblioteca.unesp.br/exlibris/bd/cathedra/08-07-2016/000865259.pdf33004064006P89420249100835492Alephreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPporinfo:eu-repo/semantics/openAccess2024-09-02T17:38:31Zoai:repositorio.unesp.br:11449/142996Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestrepositoriounesp@unesp.bropendoar:29462024-09-02T17:38:31Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Implante dérmico acelular sintético no subcutâneo e na superfície da pele de cobaias |
title |
Implante dérmico acelular sintético no subcutâneo e na superfície da pele de cobaias |
spellingShingle |
Implante dérmico acelular sintético no subcutâneo e na superfície da pele de cobaias Natsuaki, Kryscia Leiko [UNESP] Pele Implantes artificiais Cobaias Celulose bacteriana Celulose - Biodegradação Cellulose - Biodegradation |
title_short |
Implante dérmico acelular sintético no subcutâneo e na superfície da pele de cobaias |
title_full |
Implante dérmico acelular sintético no subcutâneo e na superfície da pele de cobaias |
title_fullStr |
Implante dérmico acelular sintético no subcutâneo e na superfície da pele de cobaias |
title_full_unstemmed |
Implante dérmico acelular sintético no subcutâneo e na superfície da pele de cobaias |
title_sort |
Implante dérmico acelular sintético no subcutâneo e na superfície da pele de cobaias |
author |
Natsuaki, Kryscia Leiko [UNESP] |
author_facet |
Natsuaki, Kryscia Leiko [UNESP] |
author_role |
author |
dc.contributor.none.fl_str_mv |
Schellini, Silvana Artioli [UNESP] Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Natsuaki, Kryscia Leiko [UNESP] |
dc.subject.por.fl_str_mv |
Pele Implantes artificiais Cobaias Celulose bacteriana Celulose - Biodegradação Cellulose - Biodegradation |
topic |
Pele Implantes artificiais Cobaias Celulose bacteriana Celulose - Biodegradação Cellulose - Biodegradation |
description |
The Nanoskin® is a bacterial cellulose film produced by the bacteria Acetobacter xylinum by means of a biotechnological process. It is composed by a network of nanofibrils whose structure creates a large surface area, which allows the retention of a large amount of water and significant changes in its shape without losing its structural characteristics. Purpose: aiming to add new possibilities for the reconstruction of the eyelid, in its superficial or deep lamellae, this study was developed in order to assess whether the Nanoskin® film could be an option. Method: 40 male guinea pig were used, that received Nanoskin® fragments in the dorsal region, in two studies, one directed to the use of Nanoskin® in deep tissue when the biomaterial was placed subcutaneously and another in which the Nanoskin® was placed on skin surface. In both studies we used two types of Nanoskin: group 1 (G1), which was used Nanoskin® film (2x2 cm) without gelatin coating on the subcutaneous or on the skin surface, and Group 2 (G2), which received Nanoskin® implant (2X2 cm) with gelatin coating on the subcutaneous and on the skin surface. The control group was obtained with full-thickness skin graft placement on the size of 2X2 cm Nanoskin® adjacent to the implant in all animals in G1 and G2 experiment that studied the biomaterial on the surface of the skin. Five animals from each group were euthanized at four experimental times: 7 days (M1), 30 days (M2), 90 days (M3) and 180 days (M4). Morphometric assessments of the implant and the histological slides were held, histological and ultrastructural examination. Results: the Nanoskin® when implanted subcutaneously was not found in one animal from M3 and in five animals from M4. In moments M3, M4 and M5, there was separation between their lamellae . There was a significant inflammation at the beginning of the experiment which reduced the following times, and formation of a pseudocapsule around the ... |
publishDate |
2015 |
dc.date.none.fl_str_mv |
2015-07-16 2016-08-12T18:48:46Z 2016-08-12T18:48:46Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/doctoralThesis |
format |
doctoralThesis |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
NATSUAKI, Kryscia Leiko. Implante dérmico acelular sintético no subcutâneo e na superfície da pele de cobaias. 2015. 94 f. Tese (doutorado) - Universidade Estadual Paulista Júlio de Mesquita Filho, Faculdade de Medicina de Botucatu, 2015. http://hdl.handle.net/11449/142996 000865259 http://www.athena.biblioteca.unesp.br/exlibris/bd/cathedra/08-07-2016/000865259.pdf 33004064006P8 9420249100835492 |
identifier_str_mv |
NATSUAKI, Kryscia Leiko. Implante dérmico acelular sintético no subcutâneo e na superfície da pele de cobaias. 2015. 94 f. Tese (doutorado) - Universidade Estadual Paulista Júlio de Mesquita Filho, Faculdade de Medicina de Botucatu, 2015. 000865259 33004064006P8 9420249100835492 |
url |
http://hdl.handle.net/11449/142996 http://www.athena.biblioteca.unesp.br/exlibris/bd/cathedra/08-07-2016/000865259.pdf |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
94 f. application/pdf |
dc.publisher.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) |
publisher.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) |
dc.source.none.fl_str_mv |
Aleph reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
repositoriounesp@unesp.br |
_version_ |
1810021367266934784 |