Spermatogenic cycle length and spermatogenic efficiency in the gerbil (Meriones unguiculatus)
Autor(a) principal: | |
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Data de Publicação: | 2004 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1002/j.1939-4640.2004.tb03156.x http://onlinelibrary.wiley.com/doi/10.1002/j.1939-4640.2004.tb03156.x/abstract http://hdl.handle.net/11449/243891 |
Resumo: | The gerbil (Meriones unguiculatus) is a rodent native of the arid regions of Mongolia and China. Because the gerbil can be easily bred in laboratory conditions, this species has been largely used as an experimental model in biomedical research. However, there is still little information concerning the testis structure and function in the gerbil. In this regard, we performed a detailed morphofunctional analysis of the gerbil testis and estimated the spermatogenic cycle length utilizing 3H-thymidine as a marker for germ cell progression during their evolution through the spermatogenic process. The stage frequencies of the XII stages characterized according to the acrosome formation and development were (I-XII) 13.8, 10.1, 8.1, 7.8, 4.0, 11.2, 7.5, 7.1, 5.9, 7.6, 8.1, and 8.9. The mean duration of each seminiferous epithelium cycle was determined to be 10.6 ± 1.0 days and the total duration of spermatogenesis, based on 4.5 cycles, was approximately 47.5 days. The volume density of tubular and interstitial compartments was approximately 92% and 8%, respectively. Based on the volume occupied by seminiferous tubules in the testis and the tubular diameter, about 9 and 18 m of seminiferous tubules were found per testis and per gram of testis, respectively. Twelve primary spermatocytes were formed from each type A1 spermatogonia. The meiotic index was 2.8, indicating that 30% of cell loss occurs during meiosis. The number of Leydig and Sertoli cells per gram of the testis was 28 million and each Sertoli cell was able to support approximately 13 spermatids. The daily sperm production per gram of testis (spermatogenic efficiency) was 33 million. Taken together, these data indicate that, mainly due to the high seminiferous tubule volume density and Sertoli cell support capacity for germ cells, the gerbil presents high spermatogenic efficiency compared with other mammalian species already investigated. The data obtained in the present study might provide the basis for future research involving the reproductive biology in this species. |
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Spermatogenic cycle length and spermatogenic efficiency in the gerbil (Meriones unguiculatus)MorphometrySperm productionSpermatogenesisTestisThe gerbil (Meriones unguiculatus) is a rodent native of the arid regions of Mongolia and China. Because the gerbil can be easily bred in laboratory conditions, this species has been largely used as an experimental model in biomedical research. However, there is still little information concerning the testis structure and function in the gerbil. In this regard, we performed a detailed morphofunctional analysis of the gerbil testis and estimated the spermatogenic cycle length utilizing 3H-thymidine as a marker for germ cell progression during their evolution through the spermatogenic process. The stage frequencies of the XII stages characterized according to the acrosome formation and development were (I-XII) 13.8, 10.1, 8.1, 7.8, 4.0, 11.2, 7.5, 7.1, 5.9, 7.6, 8.1, and 8.9. The mean duration of each seminiferous epithelium cycle was determined to be 10.6 ± 1.0 days and the total duration of spermatogenesis, based on 4.5 cycles, was approximately 47.5 days. The volume density of tubular and interstitial compartments was approximately 92% and 8%, respectively. Based on the volume occupied by seminiferous tubules in the testis and the tubular diameter, about 9 and 18 m of seminiferous tubules were found per testis and per gram of testis, respectively. Twelve primary spermatocytes were formed from each type A1 spermatogonia. The meiotic index was 2.8, indicating that 30% of cell loss occurs during meiosis. The number of Leydig and Sertoli cells per gram of the testis was 28 million and each Sertoli cell was able to support approximately 13 spermatids. The daily sperm production per gram of testis (spermatogenic efficiency) was 33 million. Taken together, these data indicate that, mainly due to the high seminiferous tubule volume density and Sertoli cell support capacity for germ cells, the gerbil presents high spermatogenic efficiency compared with other mammalian species already investigated. The data obtained in the present study might provide the basis for future research involving the reproductive biology in this species.Dept. of Morphofisiological Science DCM-Bloco H-79 University of State of Paraná, ParanáDepartment of Morphology Institute of Biological Science Federal University of Minas Gerais, Belo Horizonte, MGDepartment of Anatomy Institute of Bioscience State University of São Paulo, Botucatu, SPUniv Fed Minas Gerais, Lab Cellular Biol, Dept Morphol, Inst Biol Sci, Belo Horizonte, MG, BrazilState Univ São Paulo, Dept Anat, Inst Biosci, Botucatu, SP, BrazilUFSCAR, Dept Morphol & Pathol, Lab Anat, Sao Carlos, SP, BrazilState Univ São Paulo, Dept Anat, Inst Biosci, Botucatu, SP, BrazilAmer Soc Andrology, IncUniversidade Federal de Minas Gerais (UFMG)Universidade Federal de São Carlos (UFSCar)University of State of Paraná (UNESPAR)Universidade Estadual Paulista (Unesp)Segatelli, Tânia M.França, Luiz R.Pinheiro, Patricia F. F.Almeida, Camila C. D.Martinez, MarceloMartinez, Francisco E.2022-04-29T08:44:12Z2014-05-20T15:29:02Z2022-04-29T08:44:12Z2014-05-20T15:29:02Z2004info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article872-880application/pdfhttp://dx.doi.org/10.1002/j.1939-4640.2004.tb03156.xhttp://onlinelibrary.wiley.com/doi/10.1002/j.1939-4640.2004.tb03156.x/abstractJournal of Andrology, v. 25, n. 6, p. 872-880, 2004.Journal of Andrology. Lawrence: Amer Soc Andrology, Inc., v. 25, n. 6, p. 872-880, 2004.0196-3635http://hdl.handle.net/11449/24389110.1002/j.1939-4640.2004.tb03156.xWOS:0002247090000072-s2.0-70442609711739564105219382ScopusWeb of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengJournal of Andrologyinfo:eu-repo/semantics/openAccess2023-10-07T06:02:21Zoai:repositorio.unesp.br:11449/243891Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T14:12:12.560929Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Spermatogenic cycle length and spermatogenic efficiency in the gerbil (Meriones unguiculatus) |
title |
Spermatogenic cycle length and spermatogenic efficiency in the gerbil (Meriones unguiculatus) |
spellingShingle |
Spermatogenic cycle length and spermatogenic efficiency in the gerbil (Meriones unguiculatus) Segatelli, Tânia M. Morphometry Sperm production Spermatogenesis Testis |
title_short |
Spermatogenic cycle length and spermatogenic efficiency in the gerbil (Meriones unguiculatus) |
title_full |
Spermatogenic cycle length and spermatogenic efficiency in the gerbil (Meriones unguiculatus) |
title_fullStr |
Spermatogenic cycle length and spermatogenic efficiency in the gerbil (Meriones unguiculatus) |
title_full_unstemmed |
Spermatogenic cycle length and spermatogenic efficiency in the gerbil (Meriones unguiculatus) |
title_sort |
Spermatogenic cycle length and spermatogenic efficiency in the gerbil (Meriones unguiculatus) |
author |
Segatelli, Tânia M. |
author_facet |
Segatelli, Tânia M. França, Luiz R. Pinheiro, Patricia F. F. Almeida, Camila C. D. Martinez, Marcelo Martinez, Francisco E. |
author_role |
author |
author2 |
França, Luiz R. Pinheiro, Patricia F. F. Almeida, Camila C. D. Martinez, Marcelo Martinez, Francisco E. |
author2_role |
author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Federal de Minas Gerais (UFMG) Universidade Federal de São Carlos (UFSCar) University of State of Paraná (UNESPAR) Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Segatelli, Tânia M. França, Luiz R. Pinheiro, Patricia F. F. Almeida, Camila C. D. Martinez, Marcelo Martinez, Francisco E. |
dc.subject.por.fl_str_mv |
Morphometry Sperm production Spermatogenesis Testis |
topic |
Morphometry Sperm production Spermatogenesis Testis |
description |
The gerbil (Meriones unguiculatus) is a rodent native of the arid regions of Mongolia and China. Because the gerbil can be easily bred in laboratory conditions, this species has been largely used as an experimental model in biomedical research. However, there is still little information concerning the testis structure and function in the gerbil. In this regard, we performed a detailed morphofunctional analysis of the gerbil testis and estimated the spermatogenic cycle length utilizing 3H-thymidine as a marker for germ cell progression during their evolution through the spermatogenic process. The stage frequencies of the XII stages characterized according to the acrosome formation and development were (I-XII) 13.8, 10.1, 8.1, 7.8, 4.0, 11.2, 7.5, 7.1, 5.9, 7.6, 8.1, and 8.9. The mean duration of each seminiferous epithelium cycle was determined to be 10.6 ± 1.0 days and the total duration of spermatogenesis, based on 4.5 cycles, was approximately 47.5 days. The volume density of tubular and interstitial compartments was approximately 92% and 8%, respectively. Based on the volume occupied by seminiferous tubules in the testis and the tubular diameter, about 9 and 18 m of seminiferous tubules were found per testis and per gram of testis, respectively. Twelve primary spermatocytes were formed from each type A1 spermatogonia. The meiotic index was 2.8, indicating that 30% of cell loss occurs during meiosis. The number of Leydig and Sertoli cells per gram of the testis was 28 million and each Sertoli cell was able to support approximately 13 spermatids. The daily sperm production per gram of testis (spermatogenic efficiency) was 33 million. Taken together, these data indicate that, mainly due to the high seminiferous tubule volume density and Sertoli cell support capacity for germ cells, the gerbil presents high spermatogenic efficiency compared with other mammalian species already investigated. The data obtained in the present study might provide the basis for future research involving the reproductive biology in this species. |
publishDate |
2004 |
dc.date.none.fl_str_mv |
2004 2014-05-20T15:29:02Z 2014-05-20T15:29:02Z 2022-04-29T08:44:12Z 2022-04-29T08:44:12Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1002/j.1939-4640.2004.tb03156.x http://onlinelibrary.wiley.com/doi/10.1002/j.1939-4640.2004.tb03156.x/abstract Journal of Andrology, v. 25, n. 6, p. 872-880, 2004. Journal of Andrology. Lawrence: Amer Soc Andrology, Inc., v. 25, n. 6, p. 872-880, 2004. 0196-3635 http://hdl.handle.net/11449/243891 10.1002/j.1939-4640.2004.tb03156.x WOS:000224709000007 2-s2.0-7044260971 1739564105219382 |
url |
http://dx.doi.org/10.1002/j.1939-4640.2004.tb03156.x http://onlinelibrary.wiley.com/doi/10.1002/j.1939-4640.2004.tb03156.x/abstract http://hdl.handle.net/11449/243891 |
identifier_str_mv |
Journal of Andrology, v. 25, n. 6, p. 872-880, 2004. Journal of Andrology. Lawrence: Amer Soc Andrology, Inc., v. 25, n. 6, p. 872-880, 2004. 0196-3635 10.1002/j.1939-4640.2004.tb03156.x WOS:000224709000007 2-s2.0-7044260971 1739564105219382 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Journal of Andrology |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
872-880 application/pdf |
dc.publisher.none.fl_str_mv |
Amer Soc Andrology, Inc |
publisher.none.fl_str_mv |
Amer Soc Andrology, Inc |
dc.source.none.fl_str_mv |
Scopus Web of Science reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
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1808128331917295616 |