A Rapid and Eco-Friendly Method for Determination of Hydrolysable Tannins and Its Application to Honey Samples
Autor(a) principal: | |
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Data de Publicação: | 2016 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1007/s12161-016-0454-1 http://hdl.handle.net/11449/177839 |
Resumo: | This paper describes the development of a rapid and clean method for the determination of hydrolysable tannins in honey samples, focusing on an easy and green sample pretreatment and low generation of waste. The proposed method is based on the reaction between KIO3 and hydrolysable tannin compounds in acetate buffer medium (pH 4.75), with formation of a colored product measured at 525 nm. The analytical conditions were optimized using experimental design (central composite design). The linear range obtained was 40.0–740 mg L−1 (r = 0.998), and the detection (LOD) and quantification (LOQ) limits were 12.2 and 40.8 mg L−1, respectively. Acetate buffer was used to ensure formation of the colored product obtained by reaction between the chromogenic reagent and the analyte. The method showed good results when applied to honey samples, with recoveries in the range of 78.3–96.1 %, and the use of diffuse reflectance spectroscopy coupled with spot test analysis provided low reagent consumption and minimized waste generation. The results obtained with the proposed method were confirmed by a spectrophotometric method. |
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Repositório Institucional da UNESP |
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A Rapid and Eco-Friendly Method for Determination of Hydrolysable Tannins and Its Application to Honey SamplesDiffuse reflectance spectroscopyEco-friendly methodHoneyHydrolysable tanninThis paper describes the development of a rapid and clean method for the determination of hydrolysable tannins in honey samples, focusing on an easy and green sample pretreatment and low generation of waste. The proposed method is based on the reaction between KIO3 and hydrolysable tannin compounds in acetate buffer medium (pH 4.75), with formation of a colored product measured at 525 nm. The analytical conditions were optimized using experimental design (central composite design). The linear range obtained was 40.0–740 mg L−1 (r = 0.998), and the detection (LOD) and quantification (LOQ) limits were 12.2 and 40.8 mg L−1, respectively. Acetate buffer was used to ensure formation of the colored product obtained by reaction between the chromogenic reagent and the analyte. The method showed good results when applied to honey samples, with recoveries in the range of 78.3–96.1 %, and the use of diffuse reflectance spectroscopy coupled with spot test analysis provided low reagent consumption and minimized waste generation. The results obtained with the proposed method were confirmed by a spectrophotometric method.Instituto de QuÃmica Universidade Estadual Paulista “Julio de Mesquita Filho” UNESP, R. Prof. Francisco Degni 55, P.O. Box 355Instituto de QuÃmica Universidade Estadual Paulista “Julio de Mesquita Filho” UNESP, R. Prof. Francisco Degni 55, P.O. Box 355Universidade Estadual Paulista (Unesp)Catelani, Tiago Augusto [UNESP]Bittar, Dayana Borges [UNESP]Pezza, Leonardo [UNESP]Pezza, Helena Redigolo [UNESP]2018-12-11T17:27:22Z2018-12-11T17:27:22Z2016-09-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article2552-2559application/pdfhttp://dx.doi.org/10.1007/s12161-016-0454-1Food Analytical Methods, v. 9, n. 9, p. 2552-2559, 2016.1936-976X1936-9751http://hdl.handle.net/11449/17783910.1007/s12161-016-0454-12-s2.0-849591431962-s2.0-84959143196.pdf5978908591853524Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengFood Analytical Methods0,662info:eu-repo/semantics/openAccess2023-10-19T06:11:06Zoai:repositorio.unesp.br:11449/177839Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T15:23:12.983067Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
A Rapid and Eco-Friendly Method for Determination of Hydrolysable Tannins and Its Application to Honey Samples |
title |
A Rapid and Eco-Friendly Method for Determination of Hydrolysable Tannins and Its Application to Honey Samples |
spellingShingle |
A Rapid and Eco-Friendly Method for Determination of Hydrolysable Tannins and Its Application to Honey Samples Catelani, Tiago Augusto [UNESP] Diffuse reflectance spectroscopy Eco-friendly method Honey Hydrolysable tannin |
title_short |
A Rapid and Eco-Friendly Method for Determination of Hydrolysable Tannins and Its Application to Honey Samples |
title_full |
A Rapid and Eco-Friendly Method for Determination of Hydrolysable Tannins and Its Application to Honey Samples |
title_fullStr |
A Rapid and Eco-Friendly Method for Determination of Hydrolysable Tannins and Its Application to Honey Samples |
title_full_unstemmed |
A Rapid and Eco-Friendly Method for Determination of Hydrolysable Tannins and Its Application to Honey Samples |
title_sort |
A Rapid and Eco-Friendly Method for Determination of Hydrolysable Tannins and Its Application to Honey Samples |
author |
Catelani, Tiago Augusto [UNESP] |
author_facet |
Catelani, Tiago Augusto [UNESP] Bittar, Dayana Borges [UNESP] Pezza, Leonardo [UNESP] Pezza, Helena Redigolo [UNESP] |
author_role |
author |
author2 |
Bittar, Dayana Borges [UNESP] Pezza, Leonardo [UNESP] Pezza, Helena Redigolo [UNESP] |
author2_role |
author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Catelani, Tiago Augusto [UNESP] Bittar, Dayana Borges [UNESP] Pezza, Leonardo [UNESP] Pezza, Helena Redigolo [UNESP] |
dc.subject.por.fl_str_mv |
Diffuse reflectance spectroscopy Eco-friendly method Honey Hydrolysable tannin |
topic |
Diffuse reflectance spectroscopy Eco-friendly method Honey Hydrolysable tannin |
description |
This paper describes the development of a rapid and clean method for the determination of hydrolysable tannins in honey samples, focusing on an easy and green sample pretreatment and low generation of waste. The proposed method is based on the reaction between KIO3 and hydrolysable tannin compounds in acetate buffer medium (pH 4.75), with formation of a colored product measured at 525 nm. The analytical conditions were optimized using experimental design (central composite design). The linear range obtained was 40.0–740 mg L−1 (r = 0.998), and the detection (LOD) and quantification (LOQ) limits were 12.2 and 40.8 mg L−1, respectively. Acetate buffer was used to ensure formation of the colored product obtained by reaction between the chromogenic reagent and the analyte. The method showed good results when applied to honey samples, with recoveries in the range of 78.3–96.1 %, and the use of diffuse reflectance spectroscopy coupled with spot test analysis provided low reagent consumption and minimized waste generation. The results obtained with the proposed method were confirmed by a spectrophotometric method. |
publishDate |
2016 |
dc.date.none.fl_str_mv |
2016-09-01 2018-12-11T17:27:22Z 2018-12-11T17:27:22Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1007/s12161-016-0454-1 Food Analytical Methods, v. 9, n. 9, p. 2552-2559, 2016. 1936-976X 1936-9751 http://hdl.handle.net/11449/177839 10.1007/s12161-016-0454-1 2-s2.0-84959143196 2-s2.0-84959143196.pdf 5978908591853524 |
url |
http://dx.doi.org/10.1007/s12161-016-0454-1 http://hdl.handle.net/11449/177839 |
identifier_str_mv |
Food Analytical Methods, v. 9, n. 9, p. 2552-2559, 2016. 1936-976X 1936-9751 10.1007/s12161-016-0454-1 2-s2.0-84959143196 2-s2.0-84959143196.pdf 5978908591853524 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Food Analytical Methods 0,662 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
2552-2559 application/pdf |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808128506326941696 |