In vitro and in vivo evaluation of rotary-jet-spun poly(ɛ-caprolactone) with high loading of nano-hydroxyapatite

Detalhes bibliográficos
Autor(a) principal: Andrade, Telmo M.
Data de Publicação: 2019
Outros Autores: Mello, Daphne C. R. [UNESP], Elias, Conceição M. V., Abdala, Julia M. A., Silva, Edmundo [UNESP], Vasconcellos, Luana M. R. [UNESP], Tim, Carla R., Marciano, Fernanda R., Lobo, Anderson O.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1007/s10856-019-6222-1
http://hdl.handle.net/11449/228658
Resumo: Herein, poly(ɛ-caprolactone) (PCL) mats with different amounts of nanohydroxyapatite (nHAp) were produced using rotary-jet spinning (RJS) and evaluated in vitro and in vivo. The mean fiber diameters of the PCL, PCL/nHAp (3%), PCL/nHAp (5%), and PCL/nHAp (20%) scaffolds were 1847 ± 1039, 1817 ± 1044, 1294 ± 4274, and 845 ± 248 nm, respectively. Initially, all the scaffolds showed superhydrophobic behavior (contact angle around of 140 o C), but decreased to 80° after 30 min. All the produced scaffolds were bioactive after soaking in simulated body fluid, especially PCL/nHAp (20%). The crystallinity of the PCL scaffolds decreased progressively from 46 to 21% after incorporation of 20% nHAp. In vitro and in vivo cytotoxicity were investigated, as well as the mats’ ability to reduce bacteria biofilm formation. In vitro cellular differentiation was evaluated by measuring alkaline phosphatase activity and mineralized nodule formation. Overall, we identified the total ideal amount of nHAp to incorporate in PCL mats, which did not show in vitro or in vivo cytotoxicity and promoted lamellar bone formation independently of the amounts of nHAp. The scaffolds with nHAp showed reduced bacterial proliferation. Alizarin red staining was higher in materials associated with nHAp than in those without nHAp. Overall, this study demonstrates that PCL with nHAp prepared by RJS merits further evaluation for orthopedic applications.[Figure not available: see fulltext.]
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spelling In vitro and in vivo evaluation of rotary-jet-spun poly(ɛ-caprolactone) with high loading of nano-hydroxyapatiteHerein, poly(ɛ-caprolactone) (PCL) mats with different amounts of nanohydroxyapatite (nHAp) were produced using rotary-jet spinning (RJS) and evaluated in vitro and in vivo. The mean fiber diameters of the PCL, PCL/nHAp (3%), PCL/nHAp (5%), and PCL/nHAp (20%) scaffolds were 1847 ± 1039, 1817 ± 1044, 1294 ± 4274, and 845 ± 248 nm, respectively. Initially, all the scaffolds showed superhydrophobic behavior (contact angle around of 140 o C), but decreased to 80° after 30 min. All the produced scaffolds were bioactive after soaking in simulated body fluid, especially PCL/nHAp (20%). The crystallinity of the PCL scaffolds decreased progressively from 46 to 21% after incorporation of 20% nHAp. In vitro and in vivo cytotoxicity were investigated, as well as the mats’ ability to reduce bacteria biofilm formation. In vitro cellular differentiation was evaluated by measuring alkaline phosphatase activity and mineralized nodule formation. Overall, we identified the total ideal amount of nHAp to incorporate in PCL mats, which did not show in vitro or in vivo cytotoxicity and promoted lamellar bone formation independently of the amounts of nHAp. The scaffolds with nHAp showed reduced bacterial proliferation. Alizarin red staining was higher in materials associated with nHAp than in those without nHAp. Overall, this study demonstrates that PCL with nHAp prepared by RJS merits further evaluation for orthopedic applications.[Figure not available: see fulltext.]Instituto Científico e Tecnológico Universidade Brasil ItaqueraDepartamento de Biociência e Diagnóstico Oral Instituto de Ciência e Tecnologia Universidade Estadual de São Paulo, São Jose dos CamposInstituto de Pesquisa e Desenvolvimento Universidade do Vale do Paraiba, São Jose dos CamposLIMAV-Laboratório Interdisciplinar de Materiais Avançados PPGCM-Programa de Pós-graduação em Ciência e Engenharia de Materiais UFPI-Universidade Federal do PiauíDepartamento de Biociência e Diagnóstico Oral Instituto de Ciência e Tecnologia Universidade Estadual de São Paulo, São Jose dos CamposItaqueraUniversidade Estadual Paulista (UNESP)Universidade do Vale do ParaibaUFPI-Universidade Federal do PiauíAndrade, Telmo M.Mello, Daphne C. R. [UNESP]Elias, Conceição M. V.Abdala, Julia M. A.Silva, Edmundo [UNESP]Vasconcellos, Luana M. R. [UNESP]Tim, Carla R.Marciano, Fernanda R.Lobo, Anderson O.2022-04-29T08:27:56Z2022-04-29T08:27:56Z2019-02-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.1007/s10856-019-6222-1Journal of Materials Science: Materials in Medicine, v. 30, n. 2, 2019.1573-48380957-4530http://hdl.handle.net/11449/22865810.1007/s10856-019-6222-12-s2.0-85060642812Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengJournal of Materials Science: Materials in Medicineinfo:eu-repo/semantics/openAccess2022-04-29T08:27:56Zoai:repositorio.unesp.br:11449/228658Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T14:51:30.176916Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv In vitro and in vivo evaluation of rotary-jet-spun poly(ɛ-caprolactone) with high loading of nano-hydroxyapatite
title In vitro and in vivo evaluation of rotary-jet-spun poly(ɛ-caprolactone) with high loading of nano-hydroxyapatite
spellingShingle In vitro and in vivo evaluation of rotary-jet-spun poly(ɛ-caprolactone) with high loading of nano-hydroxyapatite
Andrade, Telmo M.
title_short In vitro and in vivo evaluation of rotary-jet-spun poly(ɛ-caprolactone) with high loading of nano-hydroxyapatite
title_full In vitro and in vivo evaluation of rotary-jet-spun poly(ɛ-caprolactone) with high loading of nano-hydroxyapatite
title_fullStr In vitro and in vivo evaluation of rotary-jet-spun poly(ɛ-caprolactone) with high loading of nano-hydroxyapatite
title_full_unstemmed In vitro and in vivo evaluation of rotary-jet-spun poly(ɛ-caprolactone) with high loading of nano-hydroxyapatite
title_sort In vitro and in vivo evaluation of rotary-jet-spun poly(ɛ-caprolactone) with high loading of nano-hydroxyapatite
author Andrade, Telmo M.
author_facet Andrade, Telmo M.
Mello, Daphne C. R. [UNESP]
Elias, Conceição M. V.
Abdala, Julia M. A.
Silva, Edmundo [UNESP]
Vasconcellos, Luana M. R. [UNESP]
Tim, Carla R.
Marciano, Fernanda R.
Lobo, Anderson O.
author_role author
author2 Mello, Daphne C. R. [UNESP]
Elias, Conceição M. V.
Abdala, Julia M. A.
Silva, Edmundo [UNESP]
Vasconcellos, Luana M. R. [UNESP]
Tim, Carla R.
Marciano, Fernanda R.
Lobo, Anderson O.
author2_role author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Itaquera
Universidade Estadual Paulista (UNESP)
Universidade do Vale do Paraiba
UFPI-Universidade Federal do Piauí
dc.contributor.author.fl_str_mv Andrade, Telmo M.
Mello, Daphne C. R. [UNESP]
Elias, Conceição M. V.
Abdala, Julia M. A.
Silva, Edmundo [UNESP]
Vasconcellos, Luana M. R. [UNESP]
Tim, Carla R.
Marciano, Fernanda R.
Lobo, Anderson O.
description Herein, poly(ɛ-caprolactone) (PCL) mats with different amounts of nanohydroxyapatite (nHAp) were produced using rotary-jet spinning (RJS) and evaluated in vitro and in vivo. The mean fiber diameters of the PCL, PCL/nHAp (3%), PCL/nHAp (5%), and PCL/nHAp (20%) scaffolds were 1847 ± 1039, 1817 ± 1044, 1294 ± 4274, and 845 ± 248 nm, respectively. Initially, all the scaffolds showed superhydrophobic behavior (contact angle around of 140 o C), but decreased to 80° after 30 min. All the produced scaffolds were bioactive after soaking in simulated body fluid, especially PCL/nHAp (20%). The crystallinity of the PCL scaffolds decreased progressively from 46 to 21% after incorporation of 20% nHAp. In vitro and in vivo cytotoxicity were investigated, as well as the mats’ ability to reduce bacteria biofilm formation. In vitro cellular differentiation was evaluated by measuring alkaline phosphatase activity and mineralized nodule formation. Overall, we identified the total ideal amount of nHAp to incorporate in PCL mats, which did not show in vitro or in vivo cytotoxicity and promoted lamellar bone formation independently of the amounts of nHAp. The scaffolds with nHAp showed reduced bacterial proliferation. Alizarin red staining was higher in materials associated with nHAp than in those without nHAp. Overall, this study demonstrates that PCL with nHAp prepared by RJS merits further evaluation for orthopedic applications.[Figure not available: see fulltext.]
publishDate 2019
dc.date.none.fl_str_mv 2019-02-01
2022-04-29T08:27:56Z
2022-04-29T08:27:56Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1007/s10856-019-6222-1
Journal of Materials Science: Materials in Medicine, v. 30, n. 2, 2019.
1573-4838
0957-4530
http://hdl.handle.net/11449/228658
10.1007/s10856-019-6222-1
2-s2.0-85060642812
url http://dx.doi.org/10.1007/s10856-019-6222-1
http://hdl.handle.net/11449/228658
identifier_str_mv Journal of Materials Science: Materials in Medicine, v. 30, n. 2, 2019.
1573-4838
0957-4530
10.1007/s10856-019-6222-1
2-s2.0-85060642812
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Journal of Materials Science: Materials in Medicine
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
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instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
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