In vitro and in vivo evaluation of rotary-jet-spun poly(ɛ-caprolactone) with high loading of nano-hydroxyapatite
Autor(a) principal: | |
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Data de Publicação: | 2019 |
Outros Autores: | , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1007/s10856-019-6222-1 http://hdl.handle.net/11449/228658 |
Resumo: | Herein, poly(ɛ-caprolactone) (PCL) mats with different amounts of nanohydroxyapatite (nHAp) were produced using rotary-jet spinning (RJS) and evaluated in vitro and in vivo. The mean fiber diameters of the PCL, PCL/nHAp (3%), PCL/nHAp (5%), and PCL/nHAp (20%) scaffolds were 1847 ± 1039, 1817 ± 1044, 1294 ± 4274, and 845 ± 248 nm, respectively. Initially, all the scaffolds showed superhydrophobic behavior (contact angle around of 140 o C), but decreased to 80° after 30 min. All the produced scaffolds were bioactive after soaking in simulated body fluid, especially PCL/nHAp (20%). The crystallinity of the PCL scaffolds decreased progressively from 46 to 21% after incorporation of 20% nHAp. In vitro and in vivo cytotoxicity were investigated, as well as the mats’ ability to reduce bacteria biofilm formation. In vitro cellular differentiation was evaluated by measuring alkaline phosphatase activity and mineralized nodule formation. Overall, we identified the total ideal amount of nHAp to incorporate in PCL mats, which did not show in vitro or in vivo cytotoxicity and promoted lamellar bone formation independently of the amounts of nHAp. The scaffolds with nHAp showed reduced bacterial proliferation. Alizarin red staining was higher in materials associated with nHAp than in those without nHAp. Overall, this study demonstrates that PCL with nHAp prepared by RJS merits further evaluation for orthopedic applications.[Figure not available: see fulltext.] |
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In vitro and in vivo evaluation of rotary-jet-spun poly(ɛ-caprolactone) with high loading of nano-hydroxyapatiteHerein, poly(ɛ-caprolactone) (PCL) mats with different amounts of nanohydroxyapatite (nHAp) were produced using rotary-jet spinning (RJS) and evaluated in vitro and in vivo. The mean fiber diameters of the PCL, PCL/nHAp (3%), PCL/nHAp (5%), and PCL/nHAp (20%) scaffolds were 1847 ± 1039, 1817 ± 1044, 1294 ± 4274, and 845 ± 248 nm, respectively. Initially, all the scaffolds showed superhydrophobic behavior (contact angle around of 140 o C), but decreased to 80° after 30 min. All the produced scaffolds were bioactive after soaking in simulated body fluid, especially PCL/nHAp (20%). The crystallinity of the PCL scaffolds decreased progressively from 46 to 21% after incorporation of 20% nHAp. In vitro and in vivo cytotoxicity were investigated, as well as the mats’ ability to reduce bacteria biofilm formation. In vitro cellular differentiation was evaluated by measuring alkaline phosphatase activity and mineralized nodule formation. Overall, we identified the total ideal amount of nHAp to incorporate in PCL mats, which did not show in vitro or in vivo cytotoxicity and promoted lamellar bone formation independently of the amounts of nHAp. The scaffolds with nHAp showed reduced bacterial proliferation. Alizarin red staining was higher in materials associated with nHAp than in those without nHAp. Overall, this study demonstrates that PCL with nHAp prepared by RJS merits further evaluation for orthopedic applications.[Figure not available: see fulltext.]Instituto Científico e Tecnológico Universidade Brasil ItaqueraDepartamento de Biociência e Diagnóstico Oral Instituto de Ciência e Tecnologia Universidade Estadual de São Paulo, São Jose dos CamposInstituto de Pesquisa e Desenvolvimento Universidade do Vale do Paraiba, São Jose dos CamposLIMAV-Laboratório Interdisciplinar de Materiais Avançados PPGCM-Programa de Pós-graduação em Ciência e Engenharia de Materiais UFPI-Universidade Federal do PiauíDepartamento de Biociência e Diagnóstico Oral Instituto de Ciência e Tecnologia Universidade Estadual de São Paulo, São Jose dos CamposItaqueraUniversidade Estadual Paulista (UNESP)Universidade do Vale do ParaibaUFPI-Universidade Federal do PiauíAndrade, Telmo M.Mello, Daphne C. R. [UNESP]Elias, Conceição M. V.Abdala, Julia M. A.Silva, Edmundo [UNESP]Vasconcellos, Luana M. R. [UNESP]Tim, Carla R.Marciano, Fernanda R.Lobo, Anderson O.2022-04-29T08:27:56Z2022-04-29T08:27:56Z2019-02-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.1007/s10856-019-6222-1Journal of Materials Science: Materials in Medicine, v. 30, n. 2, 2019.1573-48380957-4530http://hdl.handle.net/11449/22865810.1007/s10856-019-6222-12-s2.0-85060642812Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengJournal of Materials Science: Materials in Medicineinfo:eu-repo/semantics/openAccess2022-04-29T08:27:56Zoai:repositorio.unesp.br:11449/228658Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T14:51:30.176916Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
In vitro and in vivo evaluation of rotary-jet-spun poly(ɛ-caprolactone) with high loading of nano-hydroxyapatite |
title |
In vitro and in vivo evaluation of rotary-jet-spun poly(ɛ-caprolactone) with high loading of nano-hydroxyapatite |
spellingShingle |
In vitro and in vivo evaluation of rotary-jet-spun poly(ɛ-caprolactone) with high loading of nano-hydroxyapatite Andrade, Telmo M. |
title_short |
In vitro and in vivo evaluation of rotary-jet-spun poly(ɛ-caprolactone) with high loading of nano-hydroxyapatite |
title_full |
In vitro and in vivo evaluation of rotary-jet-spun poly(ɛ-caprolactone) with high loading of nano-hydroxyapatite |
title_fullStr |
In vitro and in vivo evaluation of rotary-jet-spun poly(ɛ-caprolactone) with high loading of nano-hydroxyapatite |
title_full_unstemmed |
In vitro and in vivo evaluation of rotary-jet-spun poly(ɛ-caprolactone) with high loading of nano-hydroxyapatite |
title_sort |
In vitro and in vivo evaluation of rotary-jet-spun poly(ɛ-caprolactone) with high loading of nano-hydroxyapatite |
author |
Andrade, Telmo M. |
author_facet |
Andrade, Telmo M. Mello, Daphne C. R. [UNESP] Elias, Conceição M. V. Abdala, Julia M. A. Silva, Edmundo [UNESP] Vasconcellos, Luana M. R. [UNESP] Tim, Carla R. Marciano, Fernanda R. Lobo, Anderson O. |
author_role |
author |
author2 |
Mello, Daphne C. R. [UNESP] Elias, Conceição M. V. Abdala, Julia M. A. Silva, Edmundo [UNESP] Vasconcellos, Luana M. R. [UNESP] Tim, Carla R. Marciano, Fernanda R. Lobo, Anderson O. |
author2_role |
author author author author author author author author |
dc.contributor.none.fl_str_mv |
Itaquera Universidade Estadual Paulista (UNESP) Universidade do Vale do Paraiba UFPI-Universidade Federal do Piauí |
dc.contributor.author.fl_str_mv |
Andrade, Telmo M. Mello, Daphne C. R. [UNESP] Elias, Conceição M. V. Abdala, Julia M. A. Silva, Edmundo [UNESP] Vasconcellos, Luana M. R. [UNESP] Tim, Carla R. Marciano, Fernanda R. Lobo, Anderson O. |
description |
Herein, poly(ɛ-caprolactone) (PCL) mats with different amounts of nanohydroxyapatite (nHAp) were produced using rotary-jet spinning (RJS) and evaluated in vitro and in vivo. The mean fiber diameters of the PCL, PCL/nHAp (3%), PCL/nHAp (5%), and PCL/nHAp (20%) scaffolds were 1847 ± 1039, 1817 ± 1044, 1294 ± 4274, and 845 ± 248 nm, respectively. Initially, all the scaffolds showed superhydrophobic behavior (contact angle around of 140 o C), but decreased to 80° after 30 min. All the produced scaffolds were bioactive after soaking in simulated body fluid, especially PCL/nHAp (20%). The crystallinity of the PCL scaffolds decreased progressively from 46 to 21% after incorporation of 20% nHAp. In vitro and in vivo cytotoxicity were investigated, as well as the mats’ ability to reduce bacteria biofilm formation. In vitro cellular differentiation was evaluated by measuring alkaline phosphatase activity and mineralized nodule formation. Overall, we identified the total ideal amount of nHAp to incorporate in PCL mats, which did not show in vitro or in vivo cytotoxicity and promoted lamellar bone formation independently of the amounts of nHAp. The scaffolds with nHAp showed reduced bacterial proliferation. Alizarin red staining was higher in materials associated with nHAp than in those without nHAp. Overall, this study demonstrates that PCL with nHAp prepared by RJS merits further evaluation for orthopedic applications.[Figure not available: see fulltext.] |
publishDate |
2019 |
dc.date.none.fl_str_mv |
2019-02-01 2022-04-29T08:27:56Z 2022-04-29T08:27:56Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1007/s10856-019-6222-1 Journal of Materials Science: Materials in Medicine, v. 30, n. 2, 2019. 1573-4838 0957-4530 http://hdl.handle.net/11449/228658 10.1007/s10856-019-6222-1 2-s2.0-85060642812 |
url |
http://dx.doi.org/10.1007/s10856-019-6222-1 http://hdl.handle.net/11449/228658 |
identifier_str_mv |
Journal of Materials Science: Materials in Medicine, v. 30, n. 2, 2019. 1573-4838 0957-4530 10.1007/s10856-019-6222-1 2-s2.0-85060642812 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Journal of Materials Science: Materials in Medicine |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808128426509336576 |