Galectin-3: Role in ocular allergy and potential as a predictive biomarker

• Autor(a) principal: Andrade, Frans Eberth Costa
• Data de Publicação: 2018
• Outros Autores: Corrêa, Mab Pereira [UNESP], Gimenes, Alexandre Dantas, Dos Santos, Myrna Serapião, Campos, Mauro, Chammas, Roger, Gomes, José Álvaro Pereira, Gil, Cristiane Damas [UNESP]
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Repositório Institucional da UNESP
• Texto Completo: http://dx.doi.org/10.1136/bjophthalmol-2017-311473; http://hdl.handle.net/11449/177155
• topic: eosinophil; galectin; impression cytology; keratoconjunctivitis; mast cell

Aims To evaluate galectin-3 (Gal-3), a β-galactoside binding protein, as a possible biomarker in ocular allergy and further investigated the role of endogenous Gal-3 in a murine model of ovalbumin (OVA)-induced allergic conjunctivitis (AC). Methods Conjunctival impression cytology specimens from control and patients with severe vernal keratoconjunctivitis, treated or untreated, were used to evaluate Gal-3 expression by immunocytochemistry. To investigate the mechanism of action of Gal-3, OVA-immunised BALB/c male wild-type (WT) and Gal-3 null (Gal-3 -/-) mice were challenged with eye drops containing OVA on days 14-16 with a subset of animals pretreated with 0.03% tacrolimus (TC) or dexamethasone (Dex). Results Patients with AC and OVA-sensitised WT mice exhibited increased levels of Gal-3 in the conjunctiva compared with control, an effect reverted by the action of Dex and TC therapy. Twenty-four hours after the final OVA challenge, total and anti-OVA IgE levels increased significantly in the blood of OVA-sensitised WT and Gal-3 -/- mice compared with controls, supporting the efficacy of the AC model. The lack of endogenous Gal-3 exacerbated the local inflammatory response, increasing the influx of eosinophils and mast cell activation. Additionally, OVA-sensitised Gal-3 -/- animals exhibited increased CD4 + expression in the eyes as well as eotaxin, IL-4, IL-13 and interferon-3 levels in the tear fluid compared with WT animals. Conclusion Gal-3 contributes to the pathogenesis of ocular allergy and represents a relevant therapeutic target.