Anti-inflammatory effect of galectin-1 in a murine model of atopic dermatitis
Autor(a) principal: | |
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Data de Publicação: | 2017 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1007/s00109-017-1566-9 http://hdl.handle.net/11449/169886 |
Resumo: | Abstract: Atopic dermatitis (AD) is caused by both dysregulated immune responses and an impaired skin barrier. Although beta-galactoside-binding protein galectin-1 (Gal-1) has immunomodulatory effects in several inflammatory disorders, therapeutic strategies based on its anti-inflammatory properties have not been explored in AD. Thus, we evaluate pharmacological treatment with Gal-1 in the progression of an ovalbumin (OVA)-induced AD-like skin lesions. The skin of OVA-immunized male BALB/c mice was challenged with drops containing OVA on days 11, 14–18 and 21–24. Additionally, in the last week, a subset of animals was treated intraperitoneally with recombinant Gal-1 (rGal-1) or dexamethasone (Dex). Treatment with rGal-1 decreased the clinical signs of dermatitis in BALB/c mice and diminished local eotaxin and IFN-γ levels. The treatment also suppressed the infiltration of eosinophils and mast cells, which was verified by reduced expression of mouse mast cell protease 6 (mMCP6) and eosinophil peroxidase (EPX). These localized effects are associated with extracellular signal-regulated kinase (ERK) activation and downregulation of endogenous Gal-1. The inhibition of disease progression induced by rGal-1 was also correlated with reduced plasma IL-17 levels. Our results demonstrate that rGal-1 is an effective treatment for allergic skin inflammation in AD and may impact the development of novel strategies for skin inflammatory diseases. Key messages: Pharmacological treatment with rGal-1 reduces clinical signs of atopic dermatitis.Systemic treatment with rGal-1 inhibits eosinophil and mast cell influx in the skin of AD animals.rGal-1 reduced local eotaxin levels and systemic IL-17 levels.The inhibition of disease progression induced by rGal-1 was correlated with upregulation of phosphorylated ERK. |
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Anti-inflammatory effect of galectin-1 in a murine model of atopic dermatitisEosinophilERKGalectin-1Mast cellOvalbuminSkin inflammationAbstract: Atopic dermatitis (AD) is caused by both dysregulated immune responses and an impaired skin barrier. Although beta-galactoside-binding protein galectin-1 (Gal-1) has immunomodulatory effects in several inflammatory disorders, therapeutic strategies based on its anti-inflammatory properties have not been explored in AD. Thus, we evaluate pharmacological treatment with Gal-1 in the progression of an ovalbumin (OVA)-induced AD-like skin lesions. The skin of OVA-immunized male BALB/c mice was challenged with drops containing OVA on days 11, 14–18 and 21–24. Additionally, in the last week, a subset of animals was treated intraperitoneally with recombinant Gal-1 (rGal-1) or dexamethasone (Dex). Treatment with rGal-1 decreased the clinical signs of dermatitis in BALB/c mice and diminished local eotaxin and IFN-γ levels. The treatment also suppressed the infiltration of eosinophils and mast cells, which was verified by reduced expression of mouse mast cell protease 6 (mMCP6) and eosinophil peroxidase (EPX). These localized effects are associated with extracellular signal-regulated kinase (ERK) activation and downregulation of endogenous Gal-1. The inhibition of disease progression induced by rGal-1 was also correlated with reduced plasma IL-17 levels. Our results demonstrate that rGal-1 is an effective treatment for allergic skin inflammation in AD and may impact the development of novel strategies for skin inflammatory diseases. Key messages: Pharmacological treatment with rGal-1 reduces clinical signs of atopic dermatitis.Systemic treatment with rGal-1 inhibits eosinophil and mast cell influx in the skin of AD animals.rGal-1 reduced local eotaxin levels and systemic IL-17 levels.The inhibition of disease progression induced by rGal-1 was correlated with upregulation of phosphorylated ERK.Post-Graduation in Biosciences UNESP - São Paulo State UniversityDepartment of Morphology and Genetics UNIFESP - Federal University of São PauloPost-Graduation in Biosciences UNESP - São Paulo State UniversityUniversidade Estadual Paulista (Unesp)Universidade de São Paulo (USP)Corrêa, Mab Pereira [UNESP]Andrade, Frans Eberth CostaGimenes, Alexandre DantasGil, Cristiane Damas [UNESP]2018-12-11T16:48:01Z2018-12-11T16:48:01Z2017-09-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article1005-1015application/pdfhttp://dx.doi.org/10.1007/s00109-017-1566-9Journal of Molecular Medicine, v. 95, n. 9, p. 1005-1015, 2017.1432-14400946-2716http://hdl.handle.net/11449/16988610.1007/s00109-017-1566-92-s2.0-850217709412-s2.0-85021770941.pdfScopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengJournal of Molecular Medicine2,1772,177info:eu-repo/semantics/openAccess2023-11-23T06:10:18Zoai:repositorio.unesp.br:11449/169886Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T18:28:39.398054Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Anti-inflammatory effect of galectin-1 in a murine model of atopic dermatitis |
title |
Anti-inflammatory effect of galectin-1 in a murine model of atopic dermatitis |
spellingShingle |
Anti-inflammatory effect of galectin-1 in a murine model of atopic dermatitis Corrêa, Mab Pereira [UNESP] Eosinophil ERK Galectin-1 Mast cell Ovalbumin Skin inflammation |
title_short |
Anti-inflammatory effect of galectin-1 in a murine model of atopic dermatitis |
title_full |
Anti-inflammatory effect of galectin-1 in a murine model of atopic dermatitis |
title_fullStr |
Anti-inflammatory effect of galectin-1 in a murine model of atopic dermatitis |
title_full_unstemmed |
Anti-inflammatory effect of galectin-1 in a murine model of atopic dermatitis |
title_sort |
Anti-inflammatory effect of galectin-1 in a murine model of atopic dermatitis |
author |
Corrêa, Mab Pereira [UNESP] |
author_facet |
Corrêa, Mab Pereira [UNESP] Andrade, Frans Eberth Costa Gimenes, Alexandre Dantas Gil, Cristiane Damas [UNESP] |
author_role |
author |
author2 |
Andrade, Frans Eberth Costa Gimenes, Alexandre Dantas Gil, Cristiane Damas [UNESP] |
author2_role |
author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) Universidade de São Paulo (USP) |
dc.contributor.author.fl_str_mv |
Corrêa, Mab Pereira [UNESP] Andrade, Frans Eberth Costa Gimenes, Alexandre Dantas Gil, Cristiane Damas [UNESP] |
dc.subject.por.fl_str_mv |
Eosinophil ERK Galectin-1 Mast cell Ovalbumin Skin inflammation |
topic |
Eosinophil ERK Galectin-1 Mast cell Ovalbumin Skin inflammation |
description |
Abstract: Atopic dermatitis (AD) is caused by both dysregulated immune responses and an impaired skin barrier. Although beta-galactoside-binding protein galectin-1 (Gal-1) has immunomodulatory effects in several inflammatory disorders, therapeutic strategies based on its anti-inflammatory properties have not been explored in AD. Thus, we evaluate pharmacological treatment with Gal-1 in the progression of an ovalbumin (OVA)-induced AD-like skin lesions. The skin of OVA-immunized male BALB/c mice was challenged with drops containing OVA on days 11, 14–18 and 21–24. Additionally, in the last week, a subset of animals was treated intraperitoneally with recombinant Gal-1 (rGal-1) or dexamethasone (Dex). Treatment with rGal-1 decreased the clinical signs of dermatitis in BALB/c mice and diminished local eotaxin and IFN-γ levels. The treatment also suppressed the infiltration of eosinophils and mast cells, which was verified by reduced expression of mouse mast cell protease 6 (mMCP6) and eosinophil peroxidase (EPX). These localized effects are associated with extracellular signal-regulated kinase (ERK) activation and downregulation of endogenous Gal-1. The inhibition of disease progression induced by rGal-1 was also correlated with reduced plasma IL-17 levels. Our results demonstrate that rGal-1 is an effective treatment for allergic skin inflammation in AD and may impact the development of novel strategies for skin inflammatory diseases. Key messages: Pharmacological treatment with rGal-1 reduces clinical signs of atopic dermatitis.Systemic treatment with rGal-1 inhibits eosinophil and mast cell influx in the skin of AD animals.rGal-1 reduced local eotaxin levels and systemic IL-17 levels.The inhibition of disease progression induced by rGal-1 was correlated with upregulation of phosphorylated ERK. |
publishDate |
2017 |
dc.date.none.fl_str_mv |
2017-09-01 2018-12-11T16:48:01Z 2018-12-11T16:48:01Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1007/s00109-017-1566-9 Journal of Molecular Medicine, v. 95, n. 9, p. 1005-1015, 2017. 1432-1440 0946-2716 http://hdl.handle.net/11449/169886 10.1007/s00109-017-1566-9 2-s2.0-85021770941 2-s2.0-85021770941.pdf |
url |
http://dx.doi.org/10.1007/s00109-017-1566-9 http://hdl.handle.net/11449/169886 |
identifier_str_mv |
Journal of Molecular Medicine, v. 95, n. 9, p. 1005-1015, 2017. 1432-1440 0946-2716 10.1007/s00109-017-1566-9 2-s2.0-85021770941 2-s2.0-85021770941.pdf |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Journal of Molecular Medicine 2,177 2,177 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
1005-1015 application/pdf |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
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1808128937273851904 |