Transferability and use of microsatellite markers for the genetic analysis of the germplasm of some Arachis section species of the genus Arachis
Autor(a) principal: | |
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Data de Publicação: | 2006 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1590/S1415-47572006000300021 http://hdl.handle.net/11449/69094 |
Resumo: | The Arachis section is the most important of the nine sections of the genus Arachis because it includes the cultivated peanut, Arachis hypogaea. The genetic improvement of A. hypogaea using wild relatives is at an early stage of development in spite of their potential as sources of genes, including those for disease and pests resistance, that are not found in the A. hypogaea primary gene pool. Section Arachis species germplasm has been collected and maintained in gene banks and its use and effective conservation depends on our knowledge of the genetic variability contained in this material. Microsatellites are routinely used for the analysis of genetic variability because they are highly polymorphic and codominant. The objective of this study was to evaluate the transferability of microsatellite primers and the assay of genetic variability between and within the germplasm of some species of the Arachis section. Fourteen microsatellite loci developed for three different species of Arachis were analyzed and 11 (78%) were found to be polymorphic. All loci had transferability to all the species analyzed. The polymorphic loci were very informative, with expected heterozygosity per locus ranging from 0.70 to 0.94. In general, the germplasm analyzed showed wide genetic variation. © 2006 Sociedade Brasileira de Genética. |
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Repositório Institucional da UNESP |
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Transferability and use of microsatellite markers for the genetic analysis of the germplasm of some Arachis section species of the genus ArachisGenetic diversityGermplasmPeanutTransferabilityWild speciescodominancedisease resistanceDNA extractiongene amplificationgene locusgene poolgene transfergenetic analysisgenetic conservationgenetic improvementgenetic polymorphismgenetic variabilitygermplasmheterozygositymicrosatellite markernonhumannucleotide sequencepeanutpest resistanceplant developmentplant geneticspolymorphic locusspecies cultivationArachisArachis hypogaeaThe Arachis section is the most important of the nine sections of the genus Arachis because it includes the cultivated peanut, Arachis hypogaea. The genetic improvement of A. hypogaea using wild relatives is at an early stage of development in spite of their potential as sources of genes, including those for disease and pests resistance, that are not found in the A. hypogaea primary gene pool. Section Arachis species germplasm has been collected and maintained in gene banks and its use and effective conservation depends on our knowledge of the genetic variability contained in this material. Microsatellites are routinely used for the analysis of genetic variability because they are highly polymorphic and codominant. The objective of this study was to evaluate the transferability of microsatellite primers and the assay of genetic variability between and within the germplasm of some species of the Arachis section. Fourteen microsatellite loci developed for three different species of Arachis were analyzed and 11 (78%) were found to be polymorphic. All loci had transferability to all the species analyzed. The polymorphic loci were very informative, with expected heterozygosity per locus ranging from 0.70 to 0.94. In general, the germplasm analyzed showed wide genetic variation. © 2006 Sociedade Brasileira de Genética.Universidade Estadual Paulista - UNESP Laboratório de Biotecnologia e Genética Molecular Departamento de Genética, Botucatu, SPInstituto Agronômico de Campinas, Caixa Postal 28, 13001-970 Campinas, SPUniversidade Estadual Paulista - UNESP Laboratório de Biotecnologia e Genética Molecular Departamento de Genética, Botucatu, SPUniversidade Estadual Paulista (Unesp)Instituto Agronômico (IAC)Bravo, Juliana Pereira [UNESP]Hoshino, Andrea Akemi [UNESP]Angelici, Carla Maria Lara C.D. [UNESP]Lopes, Catalina Romero [UNESP]Gimenes, Marcos Aparecido [UNESP]2014-05-27T11:21:58Z2014-05-27T11:21:58Z2006-09-20info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article516-524application/pdfhttp://dx.doi.org/10.1590/S1415-47572006000300021Genetics and Molecular Biology, v. 29, n. 3, p. 516-524, 2006.1415-47571678-4685http://hdl.handle.net/11449/6909410.1590/S1415-47572006000300021S1415-47572006000300021WOS:0002403285000212-s2.0-337486520692-s2.0-33748652069.pdf6903215575686010Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengGenetics and Molecular Biology1.4930,638info:eu-repo/semantics/openAccess2023-12-24T06:13:52Zoai:repositorio.unesp.br:11449/69094Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462023-12-24T06:13:52Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Transferability and use of microsatellite markers for the genetic analysis of the germplasm of some Arachis section species of the genus Arachis |
title |
Transferability and use of microsatellite markers for the genetic analysis of the germplasm of some Arachis section species of the genus Arachis |
spellingShingle |
Transferability and use of microsatellite markers for the genetic analysis of the germplasm of some Arachis section species of the genus Arachis Bravo, Juliana Pereira [UNESP] Genetic diversity Germplasm Peanut Transferability Wild species codominance disease resistance DNA extraction gene amplification gene locus gene pool gene transfer genetic analysis genetic conservation genetic improvement genetic polymorphism genetic variability germplasm heterozygosity microsatellite marker nonhuman nucleotide sequence peanut pest resistance plant development plant genetics polymorphic locus species cultivation Arachis Arachis hypogaea |
title_short |
Transferability and use of microsatellite markers for the genetic analysis of the germplasm of some Arachis section species of the genus Arachis |
title_full |
Transferability and use of microsatellite markers for the genetic analysis of the germplasm of some Arachis section species of the genus Arachis |
title_fullStr |
Transferability and use of microsatellite markers for the genetic analysis of the germplasm of some Arachis section species of the genus Arachis |
title_full_unstemmed |
Transferability and use of microsatellite markers for the genetic analysis of the germplasm of some Arachis section species of the genus Arachis |
title_sort |
Transferability and use of microsatellite markers for the genetic analysis of the germplasm of some Arachis section species of the genus Arachis |
author |
Bravo, Juliana Pereira [UNESP] |
author_facet |
Bravo, Juliana Pereira [UNESP] Hoshino, Andrea Akemi [UNESP] Angelici, Carla Maria Lara C.D. [UNESP] Lopes, Catalina Romero [UNESP] Gimenes, Marcos Aparecido [UNESP] |
author_role |
author |
author2 |
Hoshino, Andrea Akemi [UNESP] Angelici, Carla Maria Lara C.D. [UNESP] Lopes, Catalina Romero [UNESP] Gimenes, Marcos Aparecido [UNESP] |
author2_role |
author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) Instituto Agronômico (IAC) |
dc.contributor.author.fl_str_mv |
Bravo, Juliana Pereira [UNESP] Hoshino, Andrea Akemi [UNESP] Angelici, Carla Maria Lara C.D. [UNESP] Lopes, Catalina Romero [UNESP] Gimenes, Marcos Aparecido [UNESP] |
dc.subject.por.fl_str_mv |
Genetic diversity Germplasm Peanut Transferability Wild species codominance disease resistance DNA extraction gene amplification gene locus gene pool gene transfer genetic analysis genetic conservation genetic improvement genetic polymorphism genetic variability germplasm heterozygosity microsatellite marker nonhuman nucleotide sequence peanut pest resistance plant development plant genetics polymorphic locus species cultivation Arachis Arachis hypogaea |
topic |
Genetic diversity Germplasm Peanut Transferability Wild species codominance disease resistance DNA extraction gene amplification gene locus gene pool gene transfer genetic analysis genetic conservation genetic improvement genetic polymorphism genetic variability germplasm heterozygosity microsatellite marker nonhuman nucleotide sequence peanut pest resistance plant development plant genetics polymorphic locus species cultivation Arachis Arachis hypogaea |
description |
The Arachis section is the most important of the nine sections of the genus Arachis because it includes the cultivated peanut, Arachis hypogaea. The genetic improvement of A. hypogaea using wild relatives is at an early stage of development in spite of their potential as sources of genes, including those for disease and pests resistance, that are not found in the A. hypogaea primary gene pool. Section Arachis species germplasm has been collected and maintained in gene banks and its use and effective conservation depends on our knowledge of the genetic variability contained in this material. Microsatellites are routinely used for the analysis of genetic variability because they are highly polymorphic and codominant. The objective of this study was to evaluate the transferability of microsatellite primers and the assay of genetic variability between and within the germplasm of some species of the Arachis section. Fourteen microsatellite loci developed for three different species of Arachis were analyzed and 11 (78%) were found to be polymorphic. All loci had transferability to all the species analyzed. The polymorphic loci were very informative, with expected heterozygosity per locus ranging from 0.70 to 0.94. In general, the germplasm analyzed showed wide genetic variation. © 2006 Sociedade Brasileira de Genética. |
publishDate |
2006 |
dc.date.none.fl_str_mv |
2006-09-20 2014-05-27T11:21:58Z 2014-05-27T11:21:58Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1590/S1415-47572006000300021 Genetics and Molecular Biology, v. 29, n. 3, p. 516-524, 2006. 1415-4757 1678-4685 http://hdl.handle.net/11449/69094 10.1590/S1415-47572006000300021 S1415-47572006000300021 WOS:000240328500021 2-s2.0-33748652069 2-s2.0-33748652069.pdf 6903215575686010 |
url |
http://dx.doi.org/10.1590/S1415-47572006000300021 http://hdl.handle.net/11449/69094 |
identifier_str_mv |
Genetics and Molecular Biology, v. 29, n. 3, p. 516-524, 2006. 1415-4757 1678-4685 10.1590/S1415-47572006000300021 S1415-47572006000300021 WOS:000240328500021 2-s2.0-33748652069 2-s2.0-33748652069.pdf 6903215575686010 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Genetics and Molecular Biology 1.493 0,638 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
516-524 application/pdf |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1799965387478007808 |