Transferability and use of microsatellite markers for the genetic analysis of the germplasm of some Arachis section species of the genus Arachis

Detalhes bibliográficos
Autor(a) principal: Bravo, Juliana Pereira [UNESP]
Data de Publicação: 2006
Outros Autores: Hoshino, Andrea Akemi [UNESP], Angelici, Carla Maria Lara C.D. [UNESP], Lopes, Catalina Romero [UNESP], Gimenes, Marcos Aparecido [UNESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1590/S1415-47572006000300021
http://hdl.handle.net/11449/69094
Resumo: The Arachis section is the most important of the nine sections of the genus Arachis because it includes the cultivated peanut, Arachis hypogaea. The genetic improvement of A. hypogaea using wild relatives is at an early stage of development in spite of their potential as sources of genes, including those for disease and pests resistance, that are not found in the A. hypogaea primary gene pool. Section Arachis species germplasm has been collected and maintained in gene banks and its use and effective conservation depends on our knowledge of the genetic variability contained in this material. Microsatellites are routinely used for the analysis of genetic variability because they are highly polymorphic and codominant. The objective of this study was to evaluate the transferability of microsatellite primers and the assay of genetic variability between and within the germplasm of some species of the Arachis section. Fourteen microsatellite loci developed for three different species of Arachis were analyzed and 11 (78%) were found to be polymorphic. All loci had transferability to all the species analyzed. The polymorphic loci were very informative, with expected heterozygosity per locus ranging from 0.70 to 0.94. In general, the germplasm analyzed showed wide genetic variation. © 2006 Sociedade Brasileira de Genética.
id UNSP_a297258932e6a0b635a1434d1ae2b0da
oai_identifier_str oai:repositorio.unesp.br:11449/69094
network_acronym_str UNSP
network_name_str Repositório Institucional da UNESP
repository_id_str 2946
spelling Transferability and use of microsatellite markers for the genetic analysis of the germplasm of some Arachis section species of the genus ArachisGenetic diversityGermplasmPeanutTransferabilityWild speciescodominancedisease resistanceDNA extractiongene amplificationgene locusgene poolgene transfergenetic analysisgenetic conservationgenetic improvementgenetic polymorphismgenetic variabilitygermplasmheterozygositymicrosatellite markernonhumannucleotide sequencepeanutpest resistanceplant developmentplant geneticspolymorphic locusspecies cultivationArachisArachis hypogaeaThe Arachis section is the most important of the nine sections of the genus Arachis because it includes the cultivated peanut, Arachis hypogaea. The genetic improvement of A. hypogaea using wild relatives is at an early stage of development in spite of their potential as sources of genes, including those for disease and pests resistance, that are not found in the A. hypogaea primary gene pool. Section Arachis species germplasm has been collected and maintained in gene banks and its use and effective conservation depends on our knowledge of the genetic variability contained in this material. Microsatellites are routinely used for the analysis of genetic variability because they are highly polymorphic and codominant. The objective of this study was to evaluate the transferability of microsatellite primers and the assay of genetic variability between and within the germplasm of some species of the Arachis section. Fourteen microsatellite loci developed for three different species of Arachis were analyzed and 11 (78%) were found to be polymorphic. All loci had transferability to all the species analyzed. The polymorphic loci were very informative, with expected heterozygosity per locus ranging from 0.70 to 0.94. In general, the germplasm analyzed showed wide genetic variation. © 2006 Sociedade Brasileira de Genética.Universidade Estadual Paulista - UNESP Laboratório de Biotecnologia e Genética Molecular Departamento de Genética, Botucatu, SPInstituto Agronômico de Campinas, Caixa Postal 28, 13001-970 Campinas, SPUniversidade Estadual Paulista - UNESP Laboratório de Biotecnologia e Genética Molecular Departamento de Genética, Botucatu, SPUniversidade Estadual Paulista (Unesp)Instituto Agronômico (IAC)Bravo, Juliana Pereira [UNESP]Hoshino, Andrea Akemi [UNESP]Angelici, Carla Maria Lara C.D. [UNESP]Lopes, Catalina Romero [UNESP]Gimenes, Marcos Aparecido [UNESP]2014-05-27T11:21:58Z2014-05-27T11:21:58Z2006-09-20info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article516-524application/pdfhttp://dx.doi.org/10.1590/S1415-47572006000300021Genetics and Molecular Biology, v. 29, n. 3, p. 516-524, 2006.1415-47571678-4685http://hdl.handle.net/11449/6909410.1590/S1415-47572006000300021S1415-47572006000300021WOS:0002403285000212-s2.0-337486520692-s2.0-33748652069.pdf6903215575686010Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengGenetics and Molecular Biology1.4930,638info:eu-repo/semantics/openAccess2023-12-24T06:13:52Zoai:repositorio.unesp.br:11449/69094Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462023-12-24T06:13:52Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Transferability and use of microsatellite markers for the genetic analysis of the germplasm of some Arachis section species of the genus Arachis
title Transferability and use of microsatellite markers for the genetic analysis of the germplasm of some Arachis section species of the genus Arachis
spellingShingle Transferability and use of microsatellite markers for the genetic analysis of the germplasm of some Arachis section species of the genus Arachis
Bravo, Juliana Pereira [UNESP]
Genetic diversity
Germplasm
Peanut
Transferability
Wild species
codominance
disease resistance
DNA extraction
gene amplification
gene locus
gene pool
gene transfer
genetic analysis
genetic conservation
genetic improvement
genetic polymorphism
genetic variability
germplasm
heterozygosity
microsatellite marker
nonhuman
nucleotide sequence
peanut
pest resistance
plant development
plant genetics
polymorphic locus
species cultivation
Arachis
Arachis hypogaea
title_short Transferability and use of microsatellite markers for the genetic analysis of the germplasm of some Arachis section species of the genus Arachis
title_full Transferability and use of microsatellite markers for the genetic analysis of the germplasm of some Arachis section species of the genus Arachis
title_fullStr Transferability and use of microsatellite markers for the genetic analysis of the germplasm of some Arachis section species of the genus Arachis
title_full_unstemmed Transferability and use of microsatellite markers for the genetic analysis of the germplasm of some Arachis section species of the genus Arachis
title_sort Transferability and use of microsatellite markers for the genetic analysis of the germplasm of some Arachis section species of the genus Arachis
author Bravo, Juliana Pereira [UNESP]
author_facet Bravo, Juliana Pereira [UNESP]
Hoshino, Andrea Akemi [UNESP]
Angelici, Carla Maria Lara C.D. [UNESP]
Lopes, Catalina Romero [UNESP]
Gimenes, Marcos Aparecido [UNESP]
author_role author
author2 Hoshino, Andrea Akemi [UNESP]
Angelici, Carla Maria Lara C.D. [UNESP]
Lopes, Catalina Romero [UNESP]
Gimenes, Marcos Aparecido [UNESP]
author2_role author
author
author
author
dc.contributor.none.fl_str_mv Universidade Estadual Paulista (Unesp)
Instituto Agronômico (IAC)
dc.contributor.author.fl_str_mv Bravo, Juliana Pereira [UNESP]
Hoshino, Andrea Akemi [UNESP]
Angelici, Carla Maria Lara C.D. [UNESP]
Lopes, Catalina Romero [UNESP]
Gimenes, Marcos Aparecido [UNESP]
dc.subject.por.fl_str_mv Genetic diversity
Germplasm
Peanut
Transferability
Wild species
codominance
disease resistance
DNA extraction
gene amplification
gene locus
gene pool
gene transfer
genetic analysis
genetic conservation
genetic improvement
genetic polymorphism
genetic variability
germplasm
heterozygosity
microsatellite marker
nonhuman
nucleotide sequence
peanut
pest resistance
plant development
plant genetics
polymorphic locus
species cultivation
Arachis
Arachis hypogaea
topic Genetic diversity
Germplasm
Peanut
Transferability
Wild species
codominance
disease resistance
DNA extraction
gene amplification
gene locus
gene pool
gene transfer
genetic analysis
genetic conservation
genetic improvement
genetic polymorphism
genetic variability
germplasm
heterozygosity
microsatellite marker
nonhuman
nucleotide sequence
peanut
pest resistance
plant development
plant genetics
polymorphic locus
species cultivation
Arachis
Arachis hypogaea
description The Arachis section is the most important of the nine sections of the genus Arachis because it includes the cultivated peanut, Arachis hypogaea. The genetic improvement of A. hypogaea using wild relatives is at an early stage of development in spite of their potential as sources of genes, including those for disease and pests resistance, that are not found in the A. hypogaea primary gene pool. Section Arachis species germplasm has been collected and maintained in gene banks and its use and effective conservation depends on our knowledge of the genetic variability contained in this material. Microsatellites are routinely used for the analysis of genetic variability because they are highly polymorphic and codominant. The objective of this study was to evaluate the transferability of microsatellite primers and the assay of genetic variability between and within the germplasm of some species of the Arachis section. Fourteen microsatellite loci developed for three different species of Arachis were analyzed and 11 (78%) were found to be polymorphic. All loci had transferability to all the species analyzed. The polymorphic loci were very informative, with expected heterozygosity per locus ranging from 0.70 to 0.94. In general, the germplasm analyzed showed wide genetic variation. © 2006 Sociedade Brasileira de Genética.
publishDate 2006
dc.date.none.fl_str_mv 2006-09-20
2014-05-27T11:21:58Z
2014-05-27T11:21:58Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1590/S1415-47572006000300021
Genetics and Molecular Biology, v. 29, n. 3, p. 516-524, 2006.
1415-4757
1678-4685
http://hdl.handle.net/11449/69094
10.1590/S1415-47572006000300021
S1415-47572006000300021
WOS:000240328500021
2-s2.0-33748652069
2-s2.0-33748652069.pdf
6903215575686010
url http://dx.doi.org/10.1590/S1415-47572006000300021
http://hdl.handle.net/11449/69094
identifier_str_mv Genetics and Molecular Biology, v. 29, n. 3, p. 516-524, 2006.
1415-4757
1678-4685
10.1590/S1415-47572006000300021
S1415-47572006000300021
WOS:000240328500021
2-s2.0-33748652069
2-s2.0-33748652069.pdf
6903215575686010
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Genetics and Molecular Biology
1.493
0,638
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 516-524
application/pdf
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
_version_ 1799965387478007808