Enterococcus species in the oral cavity: Prevalence, virulence factors and antimicrobial susceptibility
Autor(a) principal: | |
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Data de Publicação: | 2016 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1371/journal.pone.0163001 http://hdl.handle.net/11449/178375 |
Resumo: | Enterococci are considered as transient constituent components of the oral microbiome that may cause a variety of oral and systemic infections. As there is sparse data on the oral enterococcal prevalence, we evaluated the Enterococcus spp. and their virulence attributes including antimicrobial resistance in a healthy Brazilian cohort. A total of 240 individuals in different age groups were studied (children 4-11 yrs, adolescents 12-17 yrs, young adults 18-29 yrs, adults 30-59 yrs, elderly over 60 yrs). Oral rinses were collected and isolates were identified by API 20 Strep and confirmed by 16S rDNA sequencing. E. faecalis isolates, in particular, were evaluated for virulence attributes such as their biofilm formation potential, and susceptibility to antimicrobials and an antiseptic, chlorhexidine gluconate. A total of 40 individuals (16.6%) and 10% children, 4% adolescents, 14% young adults, 30% adults, and 25% elderly carried oral enterococci. The oral enterococcal burden in adolescents was significantly lower than in the adults (p = 0.000) and elderly (p = 0.004). The proportion of carriers was higher among females (p = 0.001). E. faecalis was the most frequent isolate in all the age groups (p = 0.000), followed by E. durans and E. faecium.Whilst all the clinical isolates were able to form biofilms, only a proportion of them were able to produce lipase (92%), hemolysin (38%), and gelatinase (39%). Of all the isolates 53.8% were resistant to tetracycline, 12.3% to amoxicillin, 16.0% to ampicillin, 20.8% to chloramphenicol and 43.4% to erythromycin. None of the isolates were resistant to vancomycin. Our data suggest that in this Brazilian cohort the oral cavity may act as a significant reservoir of rather virulent and antibiotic resistant enterococci, with an increasing degree of carriage in the adults and elderly. Hence clinicians should be cognizant of this silent reservoir of virulent enterococci that may pose a particular threat of nosocomial infection. |
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Enterococcus species in the oral cavity: Prevalence, virulence factors and antimicrobial susceptibilityEnterococci are considered as transient constituent components of the oral microbiome that may cause a variety of oral and systemic infections. As there is sparse data on the oral enterococcal prevalence, we evaluated the Enterococcus spp. and their virulence attributes including antimicrobial resistance in a healthy Brazilian cohort. A total of 240 individuals in different age groups were studied (children 4-11 yrs, adolescents 12-17 yrs, young adults 18-29 yrs, adults 30-59 yrs, elderly over 60 yrs). Oral rinses were collected and isolates were identified by API 20 Strep and confirmed by 16S rDNA sequencing. E. faecalis isolates, in particular, were evaluated for virulence attributes such as their biofilm formation potential, and susceptibility to antimicrobials and an antiseptic, chlorhexidine gluconate. A total of 40 individuals (16.6%) and 10% children, 4% adolescents, 14% young adults, 30% adults, and 25% elderly carried oral enterococci. The oral enterococcal burden in adolescents was significantly lower than in the adults (p = 0.000) and elderly (p = 0.004). The proportion of carriers was higher among females (p = 0.001). E. faecalis was the most frequent isolate in all the age groups (p = 0.000), followed by E. durans and E. faecium.Whilst all the clinical isolates were able to form biofilms, only a proportion of them were able to produce lipase (92%), hemolysin (38%), and gelatinase (39%). Of all the isolates 53.8% were resistant to tetracycline, 12.3% to amoxicillin, 16.0% to ampicillin, 20.8% to chloramphenicol and 43.4% to erythromycin. None of the isolates were resistant to vancomycin. Our data suggest that in this Brazilian cohort the oral cavity may act as a significant reservoir of rather virulent and antibiotic resistant enterococci, with an increasing degree of carriage in the adults and elderly. Hence clinicians should be cognizant of this silent reservoir of virulent enterococci that may pose a particular threat of nosocomial infection.Department of Oral Biosciences and Diagnosis Institute of Science and Technology Universidade Estadual Paulista/UNESPUQ Oral Health Centre School of Dentistry University of QueenslandDepartment of Oral Bio-Sciences Faculty of Dentistry University of Hong KongDepartment of Social Dentistry Institute of Science and Technology Universidade Estadual Paulista/UNESPDepartment of Environmental Engineering Institute of Science and Technology Universidade Estadual Paulista/UNESPDepartment of Oral Biosciences and Diagnosis Institute of Science and Technology Universidade Estadual Paulista/UNESPDepartment of Social Dentistry Institute of Science and Technology Universidade Estadual Paulista/UNESPDepartment of Environmental Engineering Institute of Science and Technology Universidade Estadual Paulista/UNESPUniversidade Estadual Paulista (Unesp)University of QueenslandUniversity of Hong KongKomiyama, Edson Yukio [UNESP]Lepesqueur, Laura Soares Souto [UNESP]Yassuda, Cinthia Gomes [UNESP]Samaranayake, Lakshman P.Parahitiyawa, Nipuna B.Balducci, Ivan [UNESP]Koga-Ito, Cristiane Yumi [UNESP]2018-12-11T17:29:59Z2018-12-11T17:29:59Z2016-09-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://dx.doi.org/10.1371/journal.pone.0163001PLoS ONE, v. 11, n. 9, 2016.1932-6203http://hdl.handle.net/11449/17837510.1371/journal.pone.01630012-s2.0-849924184172-s2.0-84992418417.pdf65435631614034210000-0002-2416-2173Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengPLoS ONE1,164info:eu-repo/semantics/openAccess2023-11-03T06:11:30Zoai:repositorio.unesp.br:11449/178375Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T16:49:04.644218Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Enterococcus species in the oral cavity: Prevalence, virulence factors and antimicrobial susceptibility |
title |
Enterococcus species in the oral cavity: Prevalence, virulence factors and antimicrobial susceptibility |
spellingShingle |
Enterococcus species in the oral cavity: Prevalence, virulence factors and antimicrobial susceptibility Komiyama, Edson Yukio [UNESP] |
title_short |
Enterococcus species in the oral cavity: Prevalence, virulence factors and antimicrobial susceptibility |
title_full |
Enterococcus species in the oral cavity: Prevalence, virulence factors and antimicrobial susceptibility |
title_fullStr |
Enterococcus species in the oral cavity: Prevalence, virulence factors and antimicrobial susceptibility |
title_full_unstemmed |
Enterococcus species in the oral cavity: Prevalence, virulence factors and antimicrobial susceptibility |
title_sort |
Enterococcus species in the oral cavity: Prevalence, virulence factors and antimicrobial susceptibility |
author |
Komiyama, Edson Yukio [UNESP] |
author_facet |
Komiyama, Edson Yukio [UNESP] Lepesqueur, Laura Soares Souto [UNESP] Yassuda, Cinthia Gomes [UNESP] Samaranayake, Lakshman P. Parahitiyawa, Nipuna B. Balducci, Ivan [UNESP] Koga-Ito, Cristiane Yumi [UNESP] |
author_role |
author |
author2 |
Lepesqueur, Laura Soares Souto [UNESP] Yassuda, Cinthia Gomes [UNESP] Samaranayake, Lakshman P. Parahitiyawa, Nipuna B. Balducci, Ivan [UNESP] Koga-Ito, Cristiane Yumi [UNESP] |
author2_role |
author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) University of Queensland University of Hong Kong |
dc.contributor.author.fl_str_mv |
Komiyama, Edson Yukio [UNESP] Lepesqueur, Laura Soares Souto [UNESP] Yassuda, Cinthia Gomes [UNESP] Samaranayake, Lakshman P. Parahitiyawa, Nipuna B. Balducci, Ivan [UNESP] Koga-Ito, Cristiane Yumi [UNESP] |
description |
Enterococci are considered as transient constituent components of the oral microbiome that may cause a variety of oral and systemic infections. As there is sparse data on the oral enterococcal prevalence, we evaluated the Enterococcus spp. and their virulence attributes including antimicrobial resistance in a healthy Brazilian cohort. A total of 240 individuals in different age groups were studied (children 4-11 yrs, adolescents 12-17 yrs, young adults 18-29 yrs, adults 30-59 yrs, elderly over 60 yrs). Oral rinses were collected and isolates were identified by API 20 Strep and confirmed by 16S rDNA sequencing. E. faecalis isolates, in particular, were evaluated for virulence attributes such as their biofilm formation potential, and susceptibility to antimicrobials and an antiseptic, chlorhexidine gluconate. A total of 40 individuals (16.6%) and 10% children, 4% adolescents, 14% young adults, 30% adults, and 25% elderly carried oral enterococci. The oral enterococcal burden in adolescents was significantly lower than in the adults (p = 0.000) and elderly (p = 0.004). The proportion of carriers was higher among females (p = 0.001). E. faecalis was the most frequent isolate in all the age groups (p = 0.000), followed by E. durans and E. faecium.Whilst all the clinical isolates were able to form biofilms, only a proportion of them were able to produce lipase (92%), hemolysin (38%), and gelatinase (39%). Of all the isolates 53.8% were resistant to tetracycline, 12.3% to amoxicillin, 16.0% to ampicillin, 20.8% to chloramphenicol and 43.4% to erythromycin. None of the isolates were resistant to vancomycin. Our data suggest that in this Brazilian cohort the oral cavity may act as a significant reservoir of rather virulent and antibiotic resistant enterococci, with an increasing degree of carriage in the adults and elderly. Hence clinicians should be cognizant of this silent reservoir of virulent enterococci that may pose a particular threat of nosocomial infection. |
publishDate |
2016 |
dc.date.none.fl_str_mv |
2016-09-01 2018-12-11T17:29:59Z 2018-12-11T17:29:59Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1371/journal.pone.0163001 PLoS ONE, v. 11, n. 9, 2016. 1932-6203 http://hdl.handle.net/11449/178375 10.1371/journal.pone.0163001 2-s2.0-84992418417 2-s2.0-84992418417.pdf 6543563161403421 0000-0002-2416-2173 |
url |
http://dx.doi.org/10.1371/journal.pone.0163001 http://hdl.handle.net/11449/178375 |
identifier_str_mv |
PLoS ONE, v. 11, n. 9, 2016. 1932-6203 10.1371/journal.pone.0163001 2-s2.0-84992418417 2-s2.0-84992418417.pdf 6543563161403421 0000-0002-2416-2173 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
PLoS ONE 1,164 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
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Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
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1808128706452914176 |