Enterococcus species in the oral cavity: Prevalence, virulence factors and antimicrobial susceptibility

Detalhes bibliográficos
Autor(a) principal: Komiyama, Edson Yukio [UNESP]
Data de Publicação: 2016
Outros Autores: Lepesqueur, Laura Soares Souto [UNESP], Yassuda, Cinthia Gomes [UNESP], Samaranayake, Lakshman P., Parahitiyawa, Nipuna B., Balducci, Ivan [UNESP], Koga-Ito, Cristiane Yumi [UNESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1371/journal.pone.0163001
http://hdl.handle.net/11449/178375
Resumo: Enterococci are considered as transient constituent components of the oral microbiome that may cause a variety of oral and systemic infections. As there is sparse data on the oral enterococcal prevalence, we evaluated the Enterococcus spp. and their virulence attributes including antimicrobial resistance in a healthy Brazilian cohort. A total of 240 individuals in different age groups were studied (children 4-11 yrs, adolescents 12-17 yrs, young adults 18-29 yrs, adults 30-59 yrs, elderly over 60 yrs). Oral rinses were collected and isolates were identified by API 20 Strep and confirmed by 16S rDNA sequencing. E. faecalis isolates, in particular, were evaluated for virulence attributes such as their biofilm formation potential, and susceptibility to antimicrobials and an antiseptic, chlorhexidine gluconate. A total of 40 individuals (16.6%) and 10% children, 4% adolescents, 14% young adults, 30% adults, and 25% elderly carried oral enterococci. The oral enterococcal burden in adolescents was significantly lower than in the adults (p = 0.000) and elderly (p = 0.004). The proportion of carriers was higher among females (p = 0.001). E. faecalis was the most frequent isolate in all the age groups (p = 0.000), followed by E. durans and E. faecium.Whilst all the clinical isolates were able to form biofilms, only a proportion of them were able to produce lipase (92%), hemolysin (38%), and gelatinase (39%). Of all the isolates 53.8% were resistant to tetracycline, 12.3% to amoxicillin, 16.0% to ampicillin, 20.8% to chloramphenicol and 43.4% to erythromycin. None of the isolates were resistant to vancomycin. Our data suggest that in this Brazilian cohort the oral cavity may act as a significant reservoir of rather virulent and antibiotic resistant enterococci, with an increasing degree of carriage in the adults and elderly. Hence clinicians should be cognizant of this silent reservoir of virulent enterococci that may pose a particular threat of nosocomial infection.
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spelling Enterococcus species in the oral cavity: Prevalence, virulence factors and antimicrobial susceptibilityEnterococci are considered as transient constituent components of the oral microbiome that may cause a variety of oral and systemic infections. As there is sparse data on the oral enterococcal prevalence, we evaluated the Enterococcus spp. and their virulence attributes including antimicrobial resistance in a healthy Brazilian cohort. A total of 240 individuals in different age groups were studied (children 4-11 yrs, adolescents 12-17 yrs, young adults 18-29 yrs, adults 30-59 yrs, elderly over 60 yrs). Oral rinses were collected and isolates were identified by API 20 Strep and confirmed by 16S rDNA sequencing. E. faecalis isolates, in particular, were evaluated for virulence attributes such as their biofilm formation potential, and susceptibility to antimicrobials and an antiseptic, chlorhexidine gluconate. A total of 40 individuals (16.6%) and 10% children, 4% adolescents, 14% young adults, 30% adults, and 25% elderly carried oral enterococci. The oral enterococcal burden in adolescents was significantly lower than in the adults (p = 0.000) and elderly (p = 0.004). The proportion of carriers was higher among females (p = 0.001). E. faecalis was the most frequent isolate in all the age groups (p = 0.000), followed by E. durans and E. faecium.Whilst all the clinical isolates were able to form biofilms, only a proportion of them were able to produce lipase (92%), hemolysin (38%), and gelatinase (39%). Of all the isolates 53.8% were resistant to tetracycline, 12.3% to amoxicillin, 16.0% to ampicillin, 20.8% to chloramphenicol and 43.4% to erythromycin. None of the isolates were resistant to vancomycin. Our data suggest that in this Brazilian cohort the oral cavity may act as a significant reservoir of rather virulent and antibiotic resistant enterococci, with an increasing degree of carriage in the adults and elderly. Hence clinicians should be cognizant of this silent reservoir of virulent enterococci that may pose a particular threat of nosocomial infection.Department of Oral Biosciences and Diagnosis Institute of Science and Technology Universidade Estadual Paulista/UNESPUQ Oral Health Centre School of Dentistry University of QueenslandDepartment of Oral Bio-Sciences Faculty of Dentistry University of Hong KongDepartment of Social Dentistry Institute of Science and Technology Universidade Estadual Paulista/UNESPDepartment of Environmental Engineering Institute of Science and Technology Universidade Estadual Paulista/UNESPDepartment of Oral Biosciences and Diagnosis Institute of Science and Technology Universidade Estadual Paulista/UNESPDepartment of Social Dentistry Institute of Science and Technology Universidade Estadual Paulista/UNESPDepartment of Environmental Engineering Institute of Science and Technology Universidade Estadual Paulista/UNESPUniversidade Estadual Paulista (Unesp)University of QueenslandUniversity of Hong KongKomiyama, Edson Yukio [UNESP]Lepesqueur, Laura Soares Souto [UNESP]Yassuda, Cinthia Gomes [UNESP]Samaranayake, Lakshman P.Parahitiyawa, Nipuna B.Balducci, Ivan [UNESP]Koga-Ito, Cristiane Yumi [UNESP]2018-12-11T17:29:59Z2018-12-11T17:29:59Z2016-09-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://dx.doi.org/10.1371/journal.pone.0163001PLoS ONE, v. 11, n. 9, 2016.1932-6203http://hdl.handle.net/11449/17837510.1371/journal.pone.01630012-s2.0-849924184172-s2.0-84992418417.pdf65435631614034210000-0002-2416-2173Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengPLoS ONE1,164info:eu-repo/semantics/openAccess2023-11-03T06:11:30Zoai:repositorio.unesp.br:11449/178375Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T16:49:04.644218Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Enterococcus species in the oral cavity: Prevalence, virulence factors and antimicrobial susceptibility
title Enterococcus species in the oral cavity: Prevalence, virulence factors and antimicrobial susceptibility
spellingShingle Enterococcus species in the oral cavity: Prevalence, virulence factors and antimicrobial susceptibility
Komiyama, Edson Yukio [UNESP]
title_short Enterococcus species in the oral cavity: Prevalence, virulence factors and antimicrobial susceptibility
title_full Enterococcus species in the oral cavity: Prevalence, virulence factors and antimicrobial susceptibility
title_fullStr Enterococcus species in the oral cavity: Prevalence, virulence factors and antimicrobial susceptibility
title_full_unstemmed Enterococcus species in the oral cavity: Prevalence, virulence factors and antimicrobial susceptibility
title_sort Enterococcus species in the oral cavity: Prevalence, virulence factors and antimicrobial susceptibility
author Komiyama, Edson Yukio [UNESP]
author_facet Komiyama, Edson Yukio [UNESP]
Lepesqueur, Laura Soares Souto [UNESP]
Yassuda, Cinthia Gomes [UNESP]
Samaranayake, Lakshman P.
Parahitiyawa, Nipuna B.
Balducci, Ivan [UNESP]
Koga-Ito, Cristiane Yumi [UNESP]
author_role author
author2 Lepesqueur, Laura Soares Souto [UNESP]
Yassuda, Cinthia Gomes [UNESP]
Samaranayake, Lakshman P.
Parahitiyawa, Nipuna B.
Balducci, Ivan [UNESP]
Koga-Ito, Cristiane Yumi [UNESP]
author2_role author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade Estadual Paulista (Unesp)
University of Queensland
University of Hong Kong
dc.contributor.author.fl_str_mv Komiyama, Edson Yukio [UNESP]
Lepesqueur, Laura Soares Souto [UNESP]
Yassuda, Cinthia Gomes [UNESP]
Samaranayake, Lakshman P.
Parahitiyawa, Nipuna B.
Balducci, Ivan [UNESP]
Koga-Ito, Cristiane Yumi [UNESP]
description Enterococci are considered as transient constituent components of the oral microbiome that may cause a variety of oral and systemic infections. As there is sparse data on the oral enterococcal prevalence, we evaluated the Enterococcus spp. and their virulence attributes including antimicrobial resistance in a healthy Brazilian cohort. A total of 240 individuals in different age groups were studied (children 4-11 yrs, adolescents 12-17 yrs, young adults 18-29 yrs, adults 30-59 yrs, elderly over 60 yrs). Oral rinses were collected and isolates were identified by API 20 Strep and confirmed by 16S rDNA sequencing. E. faecalis isolates, in particular, were evaluated for virulence attributes such as their biofilm formation potential, and susceptibility to antimicrobials and an antiseptic, chlorhexidine gluconate. A total of 40 individuals (16.6%) and 10% children, 4% adolescents, 14% young adults, 30% adults, and 25% elderly carried oral enterococci. The oral enterococcal burden in adolescents was significantly lower than in the adults (p = 0.000) and elderly (p = 0.004). The proportion of carriers was higher among females (p = 0.001). E. faecalis was the most frequent isolate in all the age groups (p = 0.000), followed by E. durans and E. faecium.Whilst all the clinical isolates were able to form biofilms, only a proportion of them were able to produce lipase (92%), hemolysin (38%), and gelatinase (39%). Of all the isolates 53.8% were resistant to tetracycline, 12.3% to amoxicillin, 16.0% to ampicillin, 20.8% to chloramphenicol and 43.4% to erythromycin. None of the isolates were resistant to vancomycin. Our data suggest that in this Brazilian cohort the oral cavity may act as a significant reservoir of rather virulent and antibiotic resistant enterococci, with an increasing degree of carriage in the adults and elderly. Hence clinicians should be cognizant of this silent reservoir of virulent enterococci that may pose a particular threat of nosocomial infection.
publishDate 2016
dc.date.none.fl_str_mv 2016-09-01
2018-12-11T17:29:59Z
2018-12-11T17:29:59Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
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status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1371/journal.pone.0163001
PLoS ONE, v. 11, n. 9, 2016.
1932-6203
http://hdl.handle.net/11449/178375
10.1371/journal.pone.0163001
2-s2.0-84992418417
2-s2.0-84992418417.pdf
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url http://dx.doi.org/10.1371/journal.pone.0163001
http://hdl.handle.net/11449/178375
identifier_str_mv PLoS ONE, v. 11, n. 9, 2016.
1932-6203
10.1371/journal.pone.0163001
2-s2.0-84992418417
2-s2.0-84992418417.pdf
6543563161403421
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dc.language.iso.fl_str_mv eng
language eng
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