Long-term therapy with intravenous zoledronate increases the number of nonattached osteoclasts

• Autor(a) principal: Nagata, Maria José H. [UNESP]
• Data de Publicação: 2017
• Outros Autores: Messora, Michel R., Antoniali, Cristina [UNESP], Fucini, Stephen E. [UNESP], de Campos, Natália [UNESP], Pola, Natália M., Santinoni, Carolina S., Furlaneto, Flávia A.C., Ervolino, Edilson [UNESP]
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Repositório Institucional da UNESP
• Texto Completo: http://dx.doi.org/10.1016/j.jcms.2017.08.011; http://hdl.handle.net/11449/175242
• topic: Bone regeneration; Tooth extraction; Zoledronic acid

The purpose of this study was to investigate the influence of long-term therapy with intravenous zoledronate (ZA) on the healing of extraction sockets in rats. Forty rats, divided into groups C (Control) and Z (Zoledronate), received intravenous injections of either saline solution or ZA for 24 weeks. Their right maxillary incisor was extracted. Euthanasia was performed at 7 or 28 days postoperative. Histomorphometric (Newly Formed Bone Area) and immunohistochemical (RANKL, OPG and TRAP) analyses were performed. Data were statistically analyzed (ANOVA, Tukey's test and Kruskal–Wallis, Dunn's Multiple Comparison test).Groups C and Z showed similar new bone area, RANKL and OPG immunolabeling. The number of TRAP-positive multinucleated cells was significantly higher in Group Z than in Group C at 28 days. A significantly higher proportion of nonattached osteoclasts were seen in Group Z than in Group C at both periods of analysis. Long-term therapy with intravenous ZA stimulated nonattached osteoclast formation in extraction sockets in rats, thus decreasing local bone resorption. However, it did not influence bone formation by osteoblasts.