Probiotic Effects of Lactobacillus paracasei 28.4 to Inhibit Streptococcus mutans in a Gellan-Based Formulation

Detalhes bibliográficos
Autor(a) principal: de Alvarenga, Janaína Araújo [UNESP]
Data de Publicação: 2021
Outros Autores: de Barros, Patrícia Pimentel [UNESP], de Camargo Ribeiro, Felipe [UNESP], Rossoni, Rodnei Dennis [UNESP], Garcia, Maíra Terra [UNESP], dos Santos Velloso, Marisol [UNESP], Shukla, Shashank, Fuchs, Beth Burgwyn, Shukla, Anita, Mylonakis, Eleftherios, Junqueira, Juliana Campos [UNESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1007/s12602-020-09712-0
http://hdl.handle.net/11449/207991
Resumo: Streptococcus mutans is considered to be a major bacterium involved in dental caries, and the control of virulence mechanisms is fundamental to prevent disease. Probiotics present a promising preventive method; however, the use of probiotics requires its incorporation into delivery materials to facilitate oral colonization. Thus, we performed a comprehensive study examining preventive effects of Lactobacillus paracasei 28.4–enriched gellan hydrogel materials to inhibit S. mutans in planktonic and biofilm states, addressing its influence in the production of extracellular polysaccharides (EPS) and altered gene expression of several cariogenic virulence factors. L. paracasei 28.4, a strain isolated from the oral cavity of a caries-free individual, was incorporated in three gellan hydrogels (0.5%, 0.75%, and 1% w/v). The pretreatment with probiotic-gellan formulations provided a release of L. paracasei cells over 24 h that was sufficient to inhibit the planktonic growth of S. mutans, independent of the gellan concentrations and pH variations. This pretreatment also had inhibitory activity against S. mutans biofilms, exhibiting a reduction of 0.57 to 1.54 log10 in CFU/mL (p < 0.0001) and a decrease of 68.8 to 71.3% in total biomass (p < 0.0001) compared with the control group. These inhibitory effects were associated with the decreased production of EPS by 80% (p < 0.0001) and the downregulation of luxS, brpA, gbpB, and gtfB genes. The gellan formulation containing L. paracasei 28.4 exhibited probiotic effects for preventing S. mutans growth, biofilm formation, and production of cariogenic factors to suggest possible use in tooth decay prevention.
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spelling Probiotic Effects of Lactobacillus paracasei 28.4 to Inhibit Streptococcus mutans in a Gellan-Based FormulationBiofilmExtracellular polysaccharidesGellan hydrogelsGene expressionLactobacillus paracaseiProbioticStreptococcus mutansStreptococcus mutans is considered to be a major bacterium involved in dental caries, and the control of virulence mechanisms is fundamental to prevent disease. Probiotics present a promising preventive method; however, the use of probiotics requires its incorporation into delivery materials to facilitate oral colonization. Thus, we performed a comprehensive study examining preventive effects of Lactobacillus paracasei 28.4–enriched gellan hydrogel materials to inhibit S. mutans in planktonic and biofilm states, addressing its influence in the production of extracellular polysaccharides (EPS) and altered gene expression of several cariogenic virulence factors. L. paracasei 28.4, a strain isolated from the oral cavity of a caries-free individual, was incorporated in three gellan hydrogels (0.5%, 0.75%, and 1% w/v). The pretreatment with probiotic-gellan formulations provided a release of L. paracasei cells over 24 h that was sufficient to inhibit the planktonic growth of S. mutans, independent of the gellan concentrations and pH variations. This pretreatment also had inhibitory activity against S. mutans biofilms, exhibiting a reduction of 0.57 to 1.54 log10 in CFU/mL (p < 0.0001) and a decrease of 68.8 to 71.3% in total biomass (p < 0.0001) compared with the control group. These inhibitory effects were associated with the decreased production of EPS by 80% (p < 0.0001) and the downregulation of luxS, brpA, gbpB, and gtfB genes. The gellan formulation containing L. paracasei 28.4 exhibited probiotic effects for preventing S. mutans growth, biofilm formation, and production of cariogenic factors to suggest possible use in tooth decay prevention.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Department of Biosciences and Oral Diagnosis Institute of Science and Technology São Paulo State University/UNESP, Av. Francisco José Longo 777, São DimasSchool of Engineering Brown UniversityRhode Island Hospital Alpert Medical School & Brown UniversityDepartment of Biosciences and Oral Diagnosis Institute of Science and Technology São Paulo State University/UNESP, Av. Francisco José Longo 777, São DimasFAPESP: 2017/15529-8CNPq: 306330/2018-0Universidade Estadual Paulista (Unesp)Brown UniversityAlpert Medical School & Brown Universityde Alvarenga, Janaína Araújo [UNESP]de Barros, Patrícia Pimentel [UNESP]de Camargo Ribeiro, Felipe [UNESP]Rossoni, Rodnei Dennis [UNESP]Garcia, Maíra Terra [UNESP]dos Santos Velloso, Marisol [UNESP]Shukla, ShashankFuchs, Beth BurgwynShukla, AnitaMylonakis, EleftheriosJunqueira, Juliana Campos [UNESP]2021-06-25T11:04:31Z2021-06-25T11:04:31Z2021-04-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article506-517http://dx.doi.org/10.1007/s12602-020-09712-0Probiotics and Antimicrobial Proteins, v. 13, n. 2, p. 506-517, 2021.1867-13141867-1306http://hdl.handle.net/11449/20799110.1007/s12602-020-09712-02-s2.0-85091604526Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengProbiotics and Antimicrobial Proteinsinfo:eu-repo/semantics/openAccess2021-10-22T12:25:01Zoai:repositorio.unesp.br:11449/207991Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T19:24:05.675287Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Probiotic Effects of Lactobacillus paracasei 28.4 to Inhibit Streptococcus mutans in a Gellan-Based Formulation
title Probiotic Effects of Lactobacillus paracasei 28.4 to Inhibit Streptococcus mutans in a Gellan-Based Formulation
spellingShingle Probiotic Effects of Lactobacillus paracasei 28.4 to Inhibit Streptococcus mutans in a Gellan-Based Formulation
de Alvarenga, Janaína Araújo [UNESP]
Biofilm
Extracellular polysaccharides
Gellan hydrogels
Gene expression
Lactobacillus paracasei
Probiotic
Streptococcus mutans
title_short Probiotic Effects of Lactobacillus paracasei 28.4 to Inhibit Streptococcus mutans in a Gellan-Based Formulation
title_full Probiotic Effects of Lactobacillus paracasei 28.4 to Inhibit Streptococcus mutans in a Gellan-Based Formulation
title_fullStr Probiotic Effects of Lactobacillus paracasei 28.4 to Inhibit Streptococcus mutans in a Gellan-Based Formulation
title_full_unstemmed Probiotic Effects of Lactobacillus paracasei 28.4 to Inhibit Streptococcus mutans in a Gellan-Based Formulation
title_sort Probiotic Effects of Lactobacillus paracasei 28.4 to Inhibit Streptococcus mutans in a Gellan-Based Formulation
author de Alvarenga, Janaína Araújo [UNESP]
author_facet de Alvarenga, Janaína Araújo [UNESP]
de Barros, Patrícia Pimentel [UNESP]
de Camargo Ribeiro, Felipe [UNESP]
Rossoni, Rodnei Dennis [UNESP]
Garcia, Maíra Terra [UNESP]
dos Santos Velloso, Marisol [UNESP]
Shukla, Shashank
Fuchs, Beth Burgwyn
Shukla, Anita
Mylonakis, Eleftherios
Junqueira, Juliana Campos [UNESP]
author_role author
author2 de Barros, Patrícia Pimentel [UNESP]
de Camargo Ribeiro, Felipe [UNESP]
Rossoni, Rodnei Dennis [UNESP]
Garcia, Maíra Terra [UNESP]
dos Santos Velloso, Marisol [UNESP]
Shukla, Shashank
Fuchs, Beth Burgwyn
Shukla, Anita
Mylonakis, Eleftherios
Junqueira, Juliana Campos [UNESP]
author2_role author
author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade Estadual Paulista (Unesp)
Brown University
Alpert Medical School & Brown University
dc.contributor.author.fl_str_mv de Alvarenga, Janaína Araújo [UNESP]
de Barros, Patrícia Pimentel [UNESP]
de Camargo Ribeiro, Felipe [UNESP]
Rossoni, Rodnei Dennis [UNESP]
Garcia, Maíra Terra [UNESP]
dos Santos Velloso, Marisol [UNESP]
Shukla, Shashank
Fuchs, Beth Burgwyn
Shukla, Anita
Mylonakis, Eleftherios
Junqueira, Juliana Campos [UNESP]
dc.subject.por.fl_str_mv Biofilm
Extracellular polysaccharides
Gellan hydrogels
Gene expression
Lactobacillus paracasei
Probiotic
Streptococcus mutans
topic Biofilm
Extracellular polysaccharides
Gellan hydrogels
Gene expression
Lactobacillus paracasei
Probiotic
Streptococcus mutans
description Streptococcus mutans is considered to be a major bacterium involved in dental caries, and the control of virulence mechanisms is fundamental to prevent disease. Probiotics present a promising preventive method; however, the use of probiotics requires its incorporation into delivery materials to facilitate oral colonization. Thus, we performed a comprehensive study examining preventive effects of Lactobacillus paracasei 28.4–enriched gellan hydrogel materials to inhibit S. mutans in planktonic and biofilm states, addressing its influence in the production of extracellular polysaccharides (EPS) and altered gene expression of several cariogenic virulence factors. L. paracasei 28.4, a strain isolated from the oral cavity of a caries-free individual, was incorporated in three gellan hydrogels (0.5%, 0.75%, and 1% w/v). The pretreatment with probiotic-gellan formulations provided a release of L. paracasei cells over 24 h that was sufficient to inhibit the planktonic growth of S. mutans, independent of the gellan concentrations and pH variations. This pretreatment also had inhibitory activity against S. mutans biofilms, exhibiting a reduction of 0.57 to 1.54 log10 in CFU/mL (p < 0.0001) and a decrease of 68.8 to 71.3% in total biomass (p < 0.0001) compared with the control group. These inhibitory effects were associated with the decreased production of EPS by 80% (p < 0.0001) and the downregulation of luxS, brpA, gbpB, and gtfB genes. The gellan formulation containing L. paracasei 28.4 exhibited probiotic effects for preventing S. mutans growth, biofilm formation, and production of cariogenic factors to suggest possible use in tooth decay prevention.
publishDate 2021
dc.date.none.fl_str_mv 2021-06-25T11:04:31Z
2021-06-25T11:04:31Z
2021-04-01
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1007/s12602-020-09712-0
Probiotics and Antimicrobial Proteins, v. 13, n. 2, p. 506-517, 2021.
1867-1314
1867-1306
http://hdl.handle.net/11449/207991
10.1007/s12602-020-09712-0
2-s2.0-85091604526
url http://dx.doi.org/10.1007/s12602-020-09712-0
http://hdl.handle.net/11449/207991
identifier_str_mv Probiotics and Antimicrobial Proteins, v. 13, n. 2, p. 506-517, 2021.
1867-1314
1867-1306
10.1007/s12602-020-09712-0
2-s2.0-85091604526
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Probiotics and Antimicrobial Proteins
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 506-517
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
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