Evidence and molecular characterization of Bartonella spp. and hemoplasmas in neotropical bats in Brazil

Detalhes bibliográficos
Autor(a) principal: Ikeda, P. [UNESP]
Data de Publicação: 2017
Outros Autores: Seki, M. C., Carrasco, A. O.T., Rudiak, L. V., Miranda, J. M.D., Gonçalves, S. M.M., Hoppe, E. G.L. [UNESP], Albuquerque, A. C.A. [UNESP], Teixeira, M. M.G., Passos, C. E. [UNESP], Werther, K. [UNESP], Machado, R. Z. [UNESP], André, M. R. [UNESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1017/S0950268817000966
http://hdl.handle.net/11449/169719
Resumo: The order Chiroptera is considered the second largest group of mammals in the world, hosting important zoonotic virus and bacteria. Bartonella and hemotropic mycoplasmas are bacteria that parasite different mammals' species, including humans, causing different clinical manifestations. The present work aimed investigating the occurrence and assessing the phylogenetic positioning of Bartonella spp. and Mycoplasma spp. in neotropical bats sampled from Brazil. Between December 2015 and April 2016, 325 blood and/or tissues samples were collected from 162 bats comprising 19 different species sampled in five states of Brazil. Out of 322 bat samples collected, while 17 (5·28%) were positive to quantitative PCR for Bartonella spp. based on nuoG gene, 45 samples (13·97%) were positive to cPCR assays for hemoplasmas based on 16S rRNA gene. While seven sequences were obtained for Bartonella (nuoG) (n = 3), gltA (n = 2), rpoB (n = 1), ftsZ (n = 1), five 16S rRNA sequences were obtained for hemoplasmas. In the phylogenetic analysis, the Bartonella sequences clustered with Bartonella genotypes detected in bats sampled in Latin America countries. All five hemoplasmas sequences clustered together as a monophyletic group by Maximum Likelihood and Bayesian Inference analyses. The present work showed the first evidence of circulation of Bartonella spp. and hemoplasmas among bats in Brazil.
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spelling Evidence and molecular characterization of Bartonella spp. and hemoplasmas in neotropical bats in BrazilBartonellaceaeChiropterahemotrophic mycoplasmasphylogenetic analysesSouth AmericaThe order Chiroptera is considered the second largest group of mammals in the world, hosting important zoonotic virus and bacteria. Bartonella and hemotropic mycoplasmas are bacteria that parasite different mammals' species, including humans, causing different clinical manifestations. The present work aimed investigating the occurrence and assessing the phylogenetic positioning of Bartonella spp. and Mycoplasma spp. in neotropical bats sampled from Brazil. Between December 2015 and April 2016, 325 blood and/or tissues samples were collected from 162 bats comprising 19 different species sampled in five states of Brazil. Out of 322 bat samples collected, while 17 (5·28%) were positive to quantitative PCR for Bartonella spp. based on nuoG gene, 45 samples (13·97%) were positive to cPCR assays for hemoplasmas based on 16S rRNA gene. While seven sequences were obtained for Bartonella (nuoG) (n = 3), gltA (n = 2), rpoB (n = 1), ftsZ (n = 1), five 16S rRNA sequences were obtained for hemoplasmas. In the phylogenetic analysis, the Bartonella sequences clustered with Bartonella genotypes detected in bats sampled in Latin America countries. All five hemoplasmas sequences clustered together as a monophyletic group by Maximum Likelihood and Bayesian Inference analyses. The present work showed the first evidence of circulation of Bartonella spp. and hemoplasmas among bats in Brazil.Faculdade de Ciências Agrárias e Veterinárias Universidade Estadual Paulista (Unesp), Via de Acesso Prof. Paulo Donato Castellane, s/nUniversidade Estadual Do Centro-Oeste Campus CEDETEGFaculdade de Medicina Veterinária e Zootecnia Universidade Estadual Paulista (Unesp)Instituto de Ciências Biomédicas Universidade de São Paulo (USP)Faculdade de Ciências Agrárias e Veterinárias Universidade Estadual Paulista (Unesp), Via de Acesso Prof. Paulo Donato Castellane, s/nFaculdade de Medicina Veterinária e Zootecnia Universidade Estadual Paulista (Unesp)Universidade Estadual Paulista (Unesp)Universidade Estadual Do Centro-OesteUniversidade de São Paulo (USP)Ikeda, P. [UNESP]Seki, M. C.Carrasco, A. O.T.Rudiak, L. V.Miranda, J. M.D.Gonçalves, S. M.M.Hoppe, E. G.L. [UNESP]Albuquerque, A. C.A. [UNESP]Teixeira, M. M.G.Passos, C. E. [UNESP]Werther, K. [UNESP]Machado, R. Z. [UNESP]André, M. R. [UNESP]2018-12-11T16:47:19Z2018-12-11T16:47:19Z2017-07-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article2038-2052application/pdfhttp://dx.doi.org/10.1017/S0950268817000966Epidemiology and Infection, v. 145, n. 10, p. 2038-2052, 2017.1469-44090950-2688http://hdl.handle.net/11449/16971910.1017/S09502688170009662-s2.0-850192146902-s2.0-85019214690.pdfScopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengEpidemiology and Infection1,1281,128info:eu-repo/semantics/openAccess2023-12-17T06:22:12Zoai:repositorio.unesp.br:11449/169719Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T20:35:03.195146Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Evidence and molecular characterization of Bartonella spp. and hemoplasmas in neotropical bats in Brazil
title Evidence and molecular characterization of Bartonella spp. and hemoplasmas in neotropical bats in Brazil
spellingShingle Evidence and molecular characterization of Bartonella spp. and hemoplasmas in neotropical bats in Brazil
Ikeda, P. [UNESP]
Bartonellaceae
Chiroptera
hemotrophic mycoplasmas
phylogenetic analyses
South America
title_short Evidence and molecular characterization of Bartonella spp. and hemoplasmas in neotropical bats in Brazil
title_full Evidence and molecular characterization of Bartonella spp. and hemoplasmas in neotropical bats in Brazil
title_fullStr Evidence and molecular characterization of Bartonella spp. and hemoplasmas in neotropical bats in Brazil
title_full_unstemmed Evidence and molecular characterization of Bartonella spp. and hemoplasmas in neotropical bats in Brazil
title_sort Evidence and molecular characterization of Bartonella spp. and hemoplasmas in neotropical bats in Brazil
author Ikeda, P. [UNESP]
author_facet Ikeda, P. [UNESP]
Seki, M. C.
Carrasco, A. O.T.
Rudiak, L. V.
Miranda, J. M.D.
Gonçalves, S. M.M.
Hoppe, E. G.L. [UNESP]
Albuquerque, A. C.A. [UNESP]
Teixeira, M. M.G.
Passos, C. E. [UNESP]
Werther, K. [UNESP]
Machado, R. Z. [UNESP]
André, M. R. [UNESP]
author_role author
author2 Seki, M. C.
Carrasco, A. O.T.
Rudiak, L. V.
Miranda, J. M.D.
Gonçalves, S. M.M.
Hoppe, E. G.L. [UNESP]
Albuquerque, A. C.A. [UNESP]
Teixeira, M. M.G.
Passos, C. E. [UNESP]
Werther, K. [UNESP]
Machado, R. Z. [UNESP]
André, M. R. [UNESP]
author2_role author
author
author
author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade Estadual Paulista (Unesp)
Universidade Estadual Do Centro-Oeste
Universidade de São Paulo (USP)
dc.contributor.author.fl_str_mv Ikeda, P. [UNESP]
Seki, M. C.
Carrasco, A. O.T.
Rudiak, L. V.
Miranda, J. M.D.
Gonçalves, S. M.M.
Hoppe, E. G.L. [UNESP]
Albuquerque, A. C.A. [UNESP]
Teixeira, M. M.G.
Passos, C. E. [UNESP]
Werther, K. [UNESP]
Machado, R. Z. [UNESP]
André, M. R. [UNESP]
dc.subject.por.fl_str_mv Bartonellaceae
Chiroptera
hemotrophic mycoplasmas
phylogenetic analyses
South America
topic Bartonellaceae
Chiroptera
hemotrophic mycoplasmas
phylogenetic analyses
South America
description The order Chiroptera is considered the second largest group of mammals in the world, hosting important zoonotic virus and bacteria. Bartonella and hemotropic mycoplasmas are bacteria that parasite different mammals' species, including humans, causing different clinical manifestations. The present work aimed investigating the occurrence and assessing the phylogenetic positioning of Bartonella spp. and Mycoplasma spp. in neotropical bats sampled from Brazil. Between December 2015 and April 2016, 325 blood and/or tissues samples were collected from 162 bats comprising 19 different species sampled in five states of Brazil. Out of 322 bat samples collected, while 17 (5·28%) were positive to quantitative PCR for Bartonella spp. based on nuoG gene, 45 samples (13·97%) were positive to cPCR assays for hemoplasmas based on 16S rRNA gene. While seven sequences were obtained for Bartonella (nuoG) (n = 3), gltA (n = 2), rpoB (n = 1), ftsZ (n = 1), five 16S rRNA sequences were obtained for hemoplasmas. In the phylogenetic analysis, the Bartonella sequences clustered with Bartonella genotypes detected in bats sampled in Latin America countries. All five hemoplasmas sequences clustered together as a monophyletic group by Maximum Likelihood and Bayesian Inference analyses. The present work showed the first evidence of circulation of Bartonella spp. and hemoplasmas among bats in Brazil.
publishDate 2017
dc.date.none.fl_str_mv 2017-07-01
2018-12-11T16:47:19Z
2018-12-11T16:47:19Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1017/S0950268817000966
Epidemiology and Infection, v. 145, n. 10, p. 2038-2052, 2017.
1469-4409
0950-2688
http://hdl.handle.net/11449/169719
10.1017/S0950268817000966
2-s2.0-85019214690
2-s2.0-85019214690.pdf
url http://dx.doi.org/10.1017/S0950268817000966
http://hdl.handle.net/11449/169719
identifier_str_mv Epidemiology and Infection, v. 145, n. 10, p. 2038-2052, 2017.
1469-4409
0950-2688
10.1017/S0950268817000966
2-s2.0-85019214690
2-s2.0-85019214690.pdf
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Epidemiology and Infection
1,128
1,128
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 2038-2052
application/pdf
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
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