Evidence and molecular characterization of Bartonella spp. and hemoplasmas in neotropical bats in Brazil
Autor(a) principal: | |
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Data de Publicação: | 2017 |
Outros Autores: | , , , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1017/S0950268817000966 http://hdl.handle.net/11449/169719 |
Resumo: | The order Chiroptera is considered the second largest group of mammals in the world, hosting important zoonotic virus and bacteria. Bartonella and hemotropic mycoplasmas are bacteria that parasite different mammals' species, including humans, causing different clinical manifestations. The present work aimed investigating the occurrence and assessing the phylogenetic positioning of Bartonella spp. and Mycoplasma spp. in neotropical bats sampled from Brazil. Between December 2015 and April 2016, 325 blood and/or tissues samples were collected from 162 bats comprising 19 different species sampled in five states of Brazil. Out of 322 bat samples collected, while 17 (5·28%) were positive to quantitative PCR for Bartonella spp. based on nuoG gene, 45 samples (13·97%) were positive to cPCR assays for hemoplasmas based on 16S rRNA gene. While seven sequences were obtained for Bartonella (nuoG) (n = 3), gltA (n = 2), rpoB (n = 1), ftsZ (n = 1), five 16S rRNA sequences were obtained for hemoplasmas. In the phylogenetic analysis, the Bartonella sequences clustered with Bartonella genotypes detected in bats sampled in Latin America countries. All five hemoplasmas sequences clustered together as a monophyletic group by Maximum Likelihood and Bayesian Inference analyses. The present work showed the first evidence of circulation of Bartonella spp. and hemoplasmas among bats in Brazil. |
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Evidence and molecular characterization of Bartonella spp. and hemoplasmas in neotropical bats in BrazilBartonellaceaeChiropterahemotrophic mycoplasmasphylogenetic analysesSouth AmericaThe order Chiroptera is considered the second largest group of mammals in the world, hosting important zoonotic virus and bacteria. Bartonella and hemotropic mycoplasmas are bacteria that parasite different mammals' species, including humans, causing different clinical manifestations. The present work aimed investigating the occurrence and assessing the phylogenetic positioning of Bartonella spp. and Mycoplasma spp. in neotropical bats sampled from Brazil. Between December 2015 and April 2016, 325 blood and/or tissues samples were collected from 162 bats comprising 19 different species sampled in five states of Brazil. Out of 322 bat samples collected, while 17 (5·28%) were positive to quantitative PCR for Bartonella spp. based on nuoG gene, 45 samples (13·97%) were positive to cPCR assays for hemoplasmas based on 16S rRNA gene. While seven sequences were obtained for Bartonella (nuoG) (n = 3), gltA (n = 2), rpoB (n = 1), ftsZ (n = 1), five 16S rRNA sequences were obtained for hemoplasmas. In the phylogenetic analysis, the Bartonella sequences clustered with Bartonella genotypes detected in bats sampled in Latin America countries. All five hemoplasmas sequences clustered together as a monophyletic group by Maximum Likelihood and Bayesian Inference analyses. The present work showed the first evidence of circulation of Bartonella spp. and hemoplasmas among bats in Brazil.Faculdade de Ciências Agrárias e Veterinárias Universidade Estadual Paulista (Unesp), Via de Acesso Prof. Paulo Donato Castellane, s/nUniversidade Estadual Do Centro-Oeste Campus CEDETEGFaculdade de Medicina Veterinária e Zootecnia Universidade Estadual Paulista (Unesp)Instituto de Ciências Biomédicas Universidade de São Paulo (USP)Faculdade de Ciências Agrárias e Veterinárias Universidade Estadual Paulista (Unesp), Via de Acesso Prof. Paulo Donato Castellane, s/nFaculdade de Medicina Veterinária e Zootecnia Universidade Estadual Paulista (Unesp)Universidade Estadual Paulista (Unesp)Universidade Estadual Do Centro-OesteUniversidade de São Paulo (USP)Ikeda, P. [UNESP]Seki, M. C.Carrasco, A. O.T.Rudiak, L. V.Miranda, J. M.D.Gonçalves, S. M.M.Hoppe, E. G.L. [UNESP]Albuquerque, A. C.A. [UNESP]Teixeira, M. M.G.Passos, C. E. [UNESP]Werther, K. [UNESP]Machado, R. Z. [UNESP]André, M. R. [UNESP]2018-12-11T16:47:19Z2018-12-11T16:47:19Z2017-07-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article2038-2052application/pdfhttp://dx.doi.org/10.1017/S0950268817000966Epidemiology and Infection, v. 145, n. 10, p. 2038-2052, 2017.1469-44090950-2688http://hdl.handle.net/11449/16971910.1017/S09502688170009662-s2.0-850192146902-s2.0-85019214690.pdfScopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengEpidemiology and Infection1,1281,128info:eu-repo/semantics/openAccess2023-12-17T06:22:12Zoai:repositorio.unesp.br:11449/169719Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T20:35:03.195146Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Evidence and molecular characterization of Bartonella spp. and hemoplasmas in neotropical bats in Brazil |
title |
Evidence and molecular characterization of Bartonella spp. and hemoplasmas in neotropical bats in Brazil |
spellingShingle |
Evidence and molecular characterization of Bartonella spp. and hemoplasmas in neotropical bats in Brazil Ikeda, P. [UNESP] Bartonellaceae Chiroptera hemotrophic mycoplasmas phylogenetic analyses South America |
title_short |
Evidence and molecular characterization of Bartonella spp. and hemoplasmas in neotropical bats in Brazil |
title_full |
Evidence and molecular characterization of Bartonella spp. and hemoplasmas in neotropical bats in Brazil |
title_fullStr |
Evidence and molecular characterization of Bartonella spp. and hemoplasmas in neotropical bats in Brazil |
title_full_unstemmed |
Evidence and molecular characterization of Bartonella spp. and hemoplasmas in neotropical bats in Brazil |
title_sort |
Evidence and molecular characterization of Bartonella spp. and hemoplasmas in neotropical bats in Brazil |
author |
Ikeda, P. [UNESP] |
author_facet |
Ikeda, P. [UNESP] Seki, M. C. Carrasco, A. O.T. Rudiak, L. V. Miranda, J. M.D. Gonçalves, S. M.M. Hoppe, E. G.L. [UNESP] Albuquerque, A. C.A. [UNESP] Teixeira, M. M.G. Passos, C. E. [UNESP] Werther, K. [UNESP] Machado, R. Z. [UNESP] André, M. R. [UNESP] |
author_role |
author |
author2 |
Seki, M. C. Carrasco, A. O.T. Rudiak, L. V. Miranda, J. M.D. Gonçalves, S. M.M. Hoppe, E. G.L. [UNESP] Albuquerque, A. C.A. [UNESP] Teixeira, M. M.G. Passos, C. E. [UNESP] Werther, K. [UNESP] Machado, R. Z. [UNESP] André, M. R. [UNESP] |
author2_role |
author author author author author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) Universidade Estadual Do Centro-Oeste Universidade de São Paulo (USP) |
dc.contributor.author.fl_str_mv |
Ikeda, P. [UNESP] Seki, M. C. Carrasco, A. O.T. Rudiak, L. V. Miranda, J. M.D. Gonçalves, S. M.M. Hoppe, E. G.L. [UNESP] Albuquerque, A. C.A. [UNESP] Teixeira, M. M.G. Passos, C. E. [UNESP] Werther, K. [UNESP] Machado, R. Z. [UNESP] André, M. R. [UNESP] |
dc.subject.por.fl_str_mv |
Bartonellaceae Chiroptera hemotrophic mycoplasmas phylogenetic analyses South America |
topic |
Bartonellaceae Chiroptera hemotrophic mycoplasmas phylogenetic analyses South America |
description |
The order Chiroptera is considered the second largest group of mammals in the world, hosting important zoonotic virus and bacteria. Bartonella and hemotropic mycoplasmas are bacteria that parasite different mammals' species, including humans, causing different clinical manifestations. The present work aimed investigating the occurrence and assessing the phylogenetic positioning of Bartonella spp. and Mycoplasma spp. in neotropical bats sampled from Brazil. Between December 2015 and April 2016, 325 blood and/or tissues samples were collected from 162 bats comprising 19 different species sampled in five states of Brazil. Out of 322 bat samples collected, while 17 (5·28%) were positive to quantitative PCR for Bartonella spp. based on nuoG gene, 45 samples (13·97%) were positive to cPCR assays for hemoplasmas based on 16S rRNA gene. While seven sequences were obtained for Bartonella (nuoG) (n = 3), gltA (n = 2), rpoB (n = 1), ftsZ (n = 1), five 16S rRNA sequences were obtained for hemoplasmas. In the phylogenetic analysis, the Bartonella sequences clustered with Bartonella genotypes detected in bats sampled in Latin America countries. All five hemoplasmas sequences clustered together as a monophyletic group by Maximum Likelihood and Bayesian Inference analyses. The present work showed the first evidence of circulation of Bartonella spp. and hemoplasmas among bats in Brazil. |
publishDate |
2017 |
dc.date.none.fl_str_mv |
2017-07-01 2018-12-11T16:47:19Z 2018-12-11T16:47:19Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1017/S0950268817000966 Epidemiology and Infection, v. 145, n. 10, p. 2038-2052, 2017. 1469-4409 0950-2688 http://hdl.handle.net/11449/169719 10.1017/S0950268817000966 2-s2.0-85019214690 2-s2.0-85019214690.pdf |
url |
http://dx.doi.org/10.1017/S0950268817000966 http://hdl.handle.net/11449/169719 |
identifier_str_mv |
Epidemiology and Infection, v. 145, n. 10, p. 2038-2052, 2017. 1469-4409 0950-2688 10.1017/S0950268817000966 2-s2.0-85019214690 2-s2.0-85019214690.pdf |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Epidemiology and Infection 1,128 1,128 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
2038-2052 application/pdf |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
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1808129223172292608 |