Canine leishmaniasis: Genome-wide analysis and antibody response to Lutzomyia longipalpis saliva
Autor(a) principal: | |
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Data de Publicação: | 2018 |
Outros Autores: | , , , , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1371/journal.pone.0197215 http://hdl.handle.net/11449/164171 |
Resumo: | The anti-inflammatory properties of sand fly saliva favor the establishment of the Leishmania infantum infection. In contrast, an antibody response against Lutzomyia longipalpis saliva is often associated with a protective cell-mediated response against canine visceral leishmaniasis. Genetic studies may demonstrate to what extent the ability to secrete antisaliva antibodies depends on genetic or environmental factors. However, the genetic basis of canine antibody response against sand fly saliva has not been assessed. The aim of this study was to identify chromosomal regions associated with the anti-Lu. longipalpis salivary IgG response in 189 dogs resident in endemic areas in order to provide information for prophylactic strategies. Dogs were classified into five groups based on serological and parasitological diagnosis and clinical evaluation. Anti-salivary gland homogenate (SGH) IgG levels were assessed by Enzyme-Linked Immunosorbent Assay (ELISA). Genomic DNA was isolated from blood samples and genotyped using a SNP chip with 173,662 single nucleotide polymorphism (SNP) markers. The following linear regression model was fitted: IgG level = mean + origin + sex + age + use of a repellent collar, and the residuals were assumed as pseudo-phenotypes for the association test between phenotypes and genotypes (GWA). A component of variance model that takes into account polygenic and sample structure effects (EMMAX) was employed for GWA. Phenotypic findings indicated that anti-SGH IgG levels remained higher in exposed and subclinically infected dogs than in severely diseased dogs even in regression model residuals. Five associated markers were identified on chromosomes 2, 20 and 31. The mapped genes included CD180 (RP105) and MITF related to the rapid activation of B lymphocytes and differentiation into antibody-secreting plasma cells. The findings pointed to chromosomal segments useful for functional confirmation studies and a search for adjuvant molecules of the anti-saliva response. |
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Canine leishmaniasis: Genome-wide analysis and antibody response to Lutzomyia longipalpis salivaThe anti-inflammatory properties of sand fly saliva favor the establishment of the Leishmania infantum infection. In contrast, an antibody response against Lutzomyia longipalpis saliva is often associated with a protective cell-mediated response against canine visceral leishmaniasis. Genetic studies may demonstrate to what extent the ability to secrete antisaliva antibodies depends on genetic or environmental factors. However, the genetic basis of canine antibody response against sand fly saliva has not been assessed. The aim of this study was to identify chromosomal regions associated with the anti-Lu. longipalpis salivary IgG response in 189 dogs resident in endemic areas in order to provide information for prophylactic strategies. Dogs were classified into five groups based on serological and parasitological diagnosis and clinical evaluation. Anti-salivary gland homogenate (SGH) IgG levels were assessed by Enzyme-Linked Immunosorbent Assay (ELISA). Genomic DNA was isolated from blood samples and genotyped using a SNP chip with 173,662 single nucleotide polymorphism (SNP) markers. The following linear regression model was fitted: IgG level = mean + origin + sex + age + use of a repellent collar, and the residuals were assumed as pseudo-phenotypes for the association test between phenotypes and genotypes (GWA). A component of variance model that takes into account polygenic and sample structure effects (EMMAX) was employed for GWA. Phenotypic findings indicated that anti-SGH IgG levels remained higher in exposed and subclinically infected dogs than in severely diseased dogs even in regression model residuals. Five associated markers were identified on chromosomes 2, 20 and 31. The mapped genes included CD180 (RP105) and MITF related to the rapid activation of B lymphocytes and differentiation into antibody-secreting plasma cells. The findings pointed to chromosomal segments useful for functional confirmation studies and a search for adjuvant molecules of the anti-saliva response.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Hospital das Clinicas-Faculdade de Medicina da Universidade de Sao Paulo (HC-FMUSP), BrazilUniv Sao Paulo, Fac Med Vet & Zootecnia, Dept Patol Vet, Sao Paulo, BrazilUniv Salvador, Escola Saude, Salvador, Ba, BrazilUniv Estadual Paulista, Fac Ciencias Agr & Vet, Dept Med Vet Prevent & Reprod Anim, Jaboticabal, SP, BrazilUniv Sao Paulo, Fac Med, Lab Patol Doencas Infecciosas, Sao Paulo, BrazilUniv Sao Paulo, Fac Med Vet & Zootecnia, Dept Clin, Sao Paulo, BrazilUniv Estadual Paulista, Fac Med Vet, Dept Clin Cirurgia & Reprod Anim, Aracatuba, SP, BrazilInst Evandro Chagas, Dept Parasitol, Ananindeua, Para, BrazilUniv Estadual Paulista, Fac Med Vet, Dept Saude Anim & Prod, Aracatuba, SP, BrazilUniv Estadual Paulista, Fac Ciencias Agr & Vet, Dept Med Vet Prevent & Reprod Anim, Jaboticabal, SP, BrazilUniv Estadual Paulista, Fac Med Vet, Dept Clin Cirurgia & Reprod Anim, Aracatuba, SP, BrazilUniv Estadual Paulista, Fac Med Vet, Dept Saude Anim & Prod, Aracatuba, SP, BrazilFAPESP: 2012/50285-9FAPESP: 2012/05847-9FAPESP: 2014/01095-8CNPq: 476479/2012-6Public Library ScienceUniversidade de São Paulo (USP)Univ SalvadorUniversidade Estadual Paulista (Unesp)Inst Evandro ChagasBatista, Luis F. S.Utsunomiya, Yuri T. [UNESP]Silva, Thais B. F.Carneiro, Mariana M.Paiva, Joyr S. F.Silva, Rafaela B.Tomokane, Thaise Y.Rossi, Claudio N.Pacheco, Acacio D. [UNESP]Torrecilha, Rafaela B. P. [UNESP]Silveira, Fernando T.Marcondes, Mary [UNESP]Nunes, Caris M. [UNESP]Laurenti, Marcia D.2018-11-26T17:51:33Z2018-11-26T17:51:33Z2018-05-09info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article14application/pdfhttp://dx.doi.org/10.1371/journal.pone.0197215Plos One. San Francisco: Public Library Science, v. 13, n. 5, 14 p., 2018.1932-6203http://hdl.handle.net/11449/16417110.1371/journal.pone.0197215WOS:000431757400068WOS000431757400068.pdf1817946671090010Web of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengPlos One1,164info:eu-repo/semantics/openAccess2024-09-04T18:03:58Zoai:repositorio.unesp.br:11449/164171Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestrepositoriounesp@unesp.bropendoar:29462024-09-04T18:03:58Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Canine leishmaniasis: Genome-wide analysis and antibody response to Lutzomyia longipalpis saliva |
title |
Canine leishmaniasis: Genome-wide analysis and antibody response to Lutzomyia longipalpis saliva |
spellingShingle |
Canine leishmaniasis: Genome-wide analysis and antibody response to Lutzomyia longipalpis saliva Batista, Luis F. S. |
title_short |
Canine leishmaniasis: Genome-wide analysis and antibody response to Lutzomyia longipalpis saliva |
title_full |
Canine leishmaniasis: Genome-wide analysis and antibody response to Lutzomyia longipalpis saliva |
title_fullStr |
Canine leishmaniasis: Genome-wide analysis and antibody response to Lutzomyia longipalpis saliva |
title_full_unstemmed |
Canine leishmaniasis: Genome-wide analysis and antibody response to Lutzomyia longipalpis saliva |
title_sort |
Canine leishmaniasis: Genome-wide analysis and antibody response to Lutzomyia longipalpis saliva |
author |
Batista, Luis F. S. |
author_facet |
Batista, Luis F. S. Utsunomiya, Yuri T. [UNESP] Silva, Thais B. F. Carneiro, Mariana M. Paiva, Joyr S. F. Silva, Rafaela B. Tomokane, Thaise Y. Rossi, Claudio N. Pacheco, Acacio D. [UNESP] Torrecilha, Rafaela B. P. [UNESP] Silveira, Fernando T. Marcondes, Mary [UNESP] Nunes, Caris M. [UNESP] Laurenti, Marcia D. |
author_role |
author |
author2 |
Utsunomiya, Yuri T. [UNESP] Silva, Thais B. F. Carneiro, Mariana M. Paiva, Joyr S. F. Silva, Rafaela B. Tomokane, Thaise Y. Rossi, Claudio N. Pacheco, Acacio D. [UNESP] Torrecilha, Rafaela B. P. [UNESP] Silveira, Fernando T. Marcondes, Mary [UNESP] Nunes, Caris M. [UNESP] Laurenti, Marcia D. |
author2_role |
author author author author author author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade de São Paulo (USP) Univ Salvador Universidade Estadual Paulista (Unesp) Inst Evandro Chagas |
dc.contributor.author.fl_str_mv |
Batista, Luis F. S. Utsunomiya, Yuri T. [UNESP] Silva, Thais B. F. Carneiro, Mariana M. Paiva, Joyr S. F. Silva, Rafaela B. Tomokane, Thaise Y. Rossi, Claudio N. Pacheco, Acacio D. [UNESP] Torrecilha, Rafaela B. P. [UNESP] Silveira, Fernando T. Marcondes, Mary [UNESP] Nunes, Caris M. [UNESP] Laurenti, Marcia D. |
description |
The anti-inflammatory properties of sand fly saliva favor the establishment of the Leishmania infantum infection. In contrast, an antibody response against Lutzomyia longipalpis saliva is often associated with a protective cell-mediated response against canine visceral leishmaniasis. Genetic studies may demonstrate to what extent the ability to secrete antisaliva antibodies depends on genetic or environmental factors. However, the genetic basis of canine antibody response against sand fly saliva has not been assessed. The aim of this study was to identify chromosomal regions associated with the anti-Lu. longipalpis salivary IgG response in 189 dogs resident in endemic areas in order to provide information for prophylactic strategies. Dogs were classified into five groups based on serological and parasitological diagnosis and clinical evaluation. Anti-salivary gland homogenate (SGH) IgG levels were assessed by Enzyme-Linked Immunosorbent Assay (ELISA). Genomic DNA was isolated from blood samples and genotyped using a SNP chip with 173,662 single nucleotide polymorphism (SNP) markers. The following linear regression model was fitted: IgG level = mean + origin + sex + age + use of a repellent collar, and the residuals were assumed as pseudo-phenotypes for the association test between phenotypes and genotypes (GWA). A component of variance model that takes into account polygenic and sample structure effects (EMMAX) was employed for GWA. Phenotypic findings indicated that anti-SGH IgG levels remained higher in exposed and subclinically infected dogs than in severely diseased dogs even in regression model residuals. Five associated markers were identified on chromosomes 2, 20 and 31. The mapped genes included CD180 (RP105) and MITF related to the rapid activation of B lymphocytes and differentiation into antibody-secreting plasma cells. The findings pointed to chromosomal segments useful for functional confirmation studies and a search for adjuvant molecules of the anti-saliva response. |
publishDate |
2018 |
dc.date.none.fl_str_mv |
2018-11-26T17:51:33Z 2018-11-26T17:51:33Z 2018-05-09 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1371/journal.pone.0197215 Plos One. San Francisco: Public Library Science, v. 13, n. 5, 14 p., 2018. 1932-6203 http://hdl.handle.net/11449/164171 10.1371/journal.pone.0197215 WOS:000431757400068 WOS000431757400068.pdf 1817946671090010 |
url |
http://dx.doi.org/10.1371/journal.pone.0197215 http://hdl.handle.net/11449/164171 |
identifier_str_mv |
Plos One. San Francisco: Public Library Science, v. 13, n. 5, 14 p., 2018. 1932-6203 10.1371/journal.pone.0197215 WOS:000431757400068 WOS000431757400068.pdf 1817946671090010 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
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Plos One 1,164 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
14 application/pdf |
dc.publisher.none.fl_str_mv |
Public Library Science |
publisher.none.fl_str_mv |
Public Library Science |
dc.source.none.fl_str_mv |
Web of Science reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
repositoriounesp@unesp.br |
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1810021391939928064 |