Characterization of mesenchymal stem cells derived from equine adipose tissue

Detalhes bibliográficos
Autor(a) principal: Carvalho, A.m. [UNESP]
Data de Publicação: 2013
Outros Autores: Yamada, A.l.m. [UNESP], Golim, M.a. [UNESP], Álvarez, L.e.c. [UNESP], Jorge, L.l. [UNESP], Conceição, M.l. [UNESP], Deffune, Elenice [UNESP], Hussni, C.a. [UNESP], Alves, A.l.g. [UNESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1590/S0102-09352013000400001
http://hdl.handle.net/11449/109867
Resumo: Stem cell therapy has shown promising results in tendinitis and osteoarthritis in equine medicine. The purpose of this work was to characterize the adipose-derived mesenchymal stem cells (AdMSCs) in horses through (1) the assessment of the capacity of progenitor cells to perform adipogenic, osteogenic and chondrogenic differentiation; and (2) flow cytometry analysis using the stemness related markers: CD44, CD90, CD105 and MHC Class II. Five mixed-breed horses, aged 2-4 years-old were used to collect adipose tissue from the base of the tail. After isolation and culture of AdMSCs, immunophenotypic characterization was performed through flow cytometry. There was a high expression of CD44, CD90 and CD105, and no expression of MHC Class II markers. The tri-lineage differentiation was confirmed by specific staining: adipogenic (Oil Red O), osteogenic (Alizarin Red), and chondrogenic (Alcian Blue). The equine AdMSCs are a promising type of adult progenitor cell for tissue engineering in veterinary medicine.
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spelling Characterization of mesenchymal stem cells derived from equine adipose tissueCaracterização das células tronco mesenquimais derivadas do tecido adiposo equinocaracterização imunofenotípicadiferenciaçãoequinotecido adiposocélula tronco mesenquimalimmunophenotypic characterizationdifferentiationequineadipose tissuemesenchymal stem cellStem cell therapy has shown promising results in tendinitis and osteoarthritis in equine medicine. The purpose of this work was to characterize the adipose-derived mesenchymal stem cells (AdMSCs) in horses through (1) the assessment of the capacity of progenitor cells to perform adipogenic, osteogenic and chondrogenic differentiation; and (2) flow cytometry analysis using the stemness related markers: CD44, CD90, CD105 and MHC Class II. Five mixed-breed horses, aged 2-4 years-old were used to collect adipose tissue from the base of the tail. After isolation and culture of AdMSCs, immunophenotypic characterization was performed through flow cytometry. There was a high expression of CD44, CD90 and CD105, and no expression of MHC Class II markers. The tri-lineage differentiation was confirmed by specific staining: adipogenic (Oil Red O), osteogenic (Alizarin Red), and chondrogenic (Alcian Blue). The equine AdMSCs are a promising type of adult progenitor cell for tissue engineering in veterinary medicine.O uso de células tronco tem demonstrado resultados promissores na terapia da tendinite e osteoartrite na medicina equina. O objetivo deste trabalho foi caracterizar as células tronco mesenquimais derivadas do tecido adiposo (AdCTMs) em cavalos através da (1) avaliação da capacidade das células progenitoras para realizar a diferenciação adipogênica, osteogênica e condrogênica; e (2) através da análise por citometria de fluxo, utilizando os marcadores stemness relacionados: CD44, CD90, CD105 e MHC de Classe II. Cinco cavalos sem raça definida, de 2 a 4 anos de idade foram utilizados para a coleta do tecido adiposo da base da cauda. Após o isolamento e cultivo das AdCTMs, a caracterização imunofenotípica foi realizada pela citometria de fluxo. Houve alta expressão dos marcadores CD44, CD90 e CD105, e não houve expressão do MHC Classe II. A diferenciação foi confirmada pela coloração específica: adipogênica (Oil Red O), osteogênico (Alizarin Red), e condrogênico (Alcian Blue). As AdCTMs são um tipo promissor de células progenitoras adulta para a engenharia tecidual na medicina veterinária.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Universidade Estadual Paulista Faculdade de Medicina Veterinária e ZootecniaUniversidade Estadual Paulista Faculdade de Medicina de BotucatuUniversidade Estadual Paulista Faculdade de Medicina Veterinária e ZootecniaUniversidade Estadual Paulista Faculdade de Medicina de BotucatuFAPESP: 09/10670-8FAPESP: 10/03567-3Universidade Federal de Minas Gerais (UFMG), Escola de VeterináriaUniversidade Estadual Paulista (Unesp)Carvalho, A.m. [UNESP]Yamada, A.l.m. [UNESP]Golim, M.a. [UNESP]Álvarez, L.e.c. [UNESP]Jorge, L.l. [UNESP]Conceição, M.l. [UNESP]Deffune, Elenice [UNESP]Hussni, C.a. [UNESP]Alves, A.l.g. [UNESP]2014-10-01T13:08:38Z2014-10-01T13:08:38Z2013-08-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article939-945application/pdfhttp://dx.doi.org/10.1590/S0102-09352013000400001Arquivo Brasileiro de Medicina Veterinária e Zootecnia. Universidade Federal de Minas Gerais, Escola de Veterinária, v. 65, n. 4, p. 939-945, 2013.0102-0935http://hdl.handle.net/11449/10986710.1590/S0102-09352013000400001S0102-09352013000400001WOS:000324292900001S0102-09352013000400001.pdf9646764071339214SciELOreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengArquivo Brasileiro de Medicina Veterinária e Zootecnia0.2860,248info:eu-repo/semantics/openAccess2024-09-03T14:29:57Zoai:repositorio.unesp.br:11449/109867Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestrepositoriounesp@unesp.bropendoar:29462024-09-03T14:29:57Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Characterization of mesenchymal stem cells derived from equine adipose tissue
Caracterização das células tronco mesenquimais derivadas do tecido adiposo equino
title Characterization of mesenchymal stem cells derived from equine adipose tissue
spellingShingle Characterization of mesenchymal stem cells derived from equine adipose tissue
Carvalho, A.m. [UNESP]
caracterização imunofenotípica
diferenciação
equino
tecido adiposo
célula tronco mesenquimal
immunophenotypic characterization
differentiation
equine
adipose tissue
mesenchymal stem cell
title_short Characterization of mesenchymal stem cells derived from equine adipose tissue
title_full Characterization of mesenchymal stem cells derived from equine adipose tissue
title_fullStr Characterization of mesenchymal stem cells derived from equine adipose tissue
title_full_unstemmed Characterization of mesenchymal stem cells derived from equine adipose tissue
title_sort Characterization of mesenchymal stem cells derived from equine adipose tissue
author Carvalho, A.m. [UNESP]
author_facet Carvalho, A.m. [UNESP]
Yamada, A.l.m. [UNESP]
Golim, M.a. [UNESP]
Álvarez, L.e.c. [UNESP]
Jorge, L.l. [UNESP]
Conceição, M.l. [UNESP]
Deffune, Elenice [UNESP]
Hussni, C.a. [UNESP]
Alves, A.l.g. [UNESP]
author_role author
author2 Yamada, A.l.m. [UNESP]
Golim, M.a. [UNESP]
Álvarez, L.e.c. [UNESP]
Jorge, L.l. [UNESP]
Conceição, M.l. [UNESP]
Deffune, Elenice [UNESP]
Hussni, C.a. [UNESP]
Alves, A.l.g. [UNESP]
author2_role author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade Estadual Paulista (Unesp)
dc.contributor.author.fl_str_mv Carvalho, A.m. [UNESP]
Yamada, A.l.m. [UNESP]
Golim, M.a. [UNESP]
Álvarez, L.e.c. [UNESP]
Jorge, L.l. [UNESP]
Conceição, M.l. [UNESP]
Deffune, Elenice [UNESP]
Hussni, C.a. [UNESP]
Alves, A.l.g. [UNESP]
dc.subject.por.fl_str_mv caracterização imunofenotípica
diferenciação
equino
tecido adiposo
célula tronco mesenquimal
immunophenotypic characterization
differentiation
equine
adipose tissue
mesenchymal stem cell
topic caracterização imunofenotípica
diferenciação
equino
tecido adiposo
célula tronco mesenquimal
immunophenotypic characterization
differentiation
equine
adipose tissue
mesenchymal stem cell
description Stem cell therapy has shown promising results in tendinitis and osteoarthritis in equine medicine. The purpose of this work was to characterize the adipose-derived mesenchymal stem cells (AdMSCs) in horses through (1) the assessment of the capacity of progenitor cells to perform adipogenic, osteogenic and chondrogenic differentiation; and (2) flow cytometry analysis using the stemness related markers: CD44, CD90, CD105 and MHC Class II. Five mixed-breed horses, aged 2-4 years-old were used to collect adipose tissue from the base of the tail. After isolation and culture of AdMSCs, immunophenotypic characterization was performed through flow cytometry. There was a high expression of CD44, CD90 and CD105, and no expression of MHC Class II markers. The tri-lineage differentiation was confirmed by specific staining: adipogenic (Oil Red O), osteogenic (Alizarin Red), and chondrogenic (Alcian Blue). The equine AdMSCs are a promising type of adult progenitor cell for tissue engineering in veterinary medicine.
publishDate 2013
dc.date.none.fl_str_mv 2013-08-01
2014-10-01T13:08:38Z
2014-10-01T13:08:38Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1590/S0102-09352013000400001
Arquivo Brasileiro de Medicina Veterinária e Zootecnia. Universidade Federal de Minas Gerais, Escola de Veterinária, v. 65, n. 4, p. 939-945, 2013.
0102-0935
http://hdl.handle.net/11449/109867
10.1590/S0102-09352013000400001
S0102-09352013000400001
WOS:000324292900001
S0102-09352013000400001.pdf
9646764071339214
url http://dx.doi.org/10.1590/S0102-09352013000400001
http://hdl.handle.net/11449/109867
identifier_str_mv Arquivo Brasileiro de Medicina Veterinária e Zootecnia. Universidade Federal de Minas Gerais, Escola de Veterinária, v. 65, n. 4, p. 939-945, 2013.
0102-0935
10.1590/S0102-09352013000400001
S0102-09352013000400001
WOS:000324292900001
S0102-09352013000400001.pdf
9646764071339214
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Arquivo Brasileiro de Medicina Veterinária e Zootecnia
0.286
0,248
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 939-945
application/pdf
dc.publisher.none.fl_str_mv Universidade Federal de Minas Gerais (UFMG), Escola de Veterinária
publisher.none.fl_str_mv Universidade Federal de Minas Gerais (UFMG), Escola de Veterinária
dc.source.none.fl_str_mv SciELO
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv repositoriounesp@unesp.br
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