Characterization of mesenchymal stem cells derived from equine adipose tissue
Autor(a) principal: | |
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Data de Publicação: | 2013 |
Outros Autores: | , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1590/S0102-09352013000400001 http://hdl.handle.net/11449/109867 |
Resumo: | Stem cell therapy has shown promising results in tendinitis and osteoarthritis in equine medicine. The purpose of this work was to characterize the adipose-derived mesenchymal stem cells (AdMSCs) in horses through (1) the assessment of the capacity of progenitor cells to perform adipogenic, osteogenic and chondrogenic differentiation; and (2) flow cytometry analysis using the stemness related markers: CD44, CD90, CD105 and MHC Class II. Five mixed-breed horses, aged 2-4 years-old were used to collect adipose tissue from the base of the tail. After isolation and culture of AdMSCs, immunophenotypic characterization was performed through flow cytometry. There was a high expression of CD44, CD90 and CD105, and no expression of MHC Class II markers. The tri-lineage differentiation was confirmed by specific staining: adipogenic (Oil Red O), osteogenic (Alizarin Red), and chondrogenic (Alcian Blue). The equine AdMSCs are a promising type of adult progenitor cell for tissue engineering in veterinary medicine. |
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Characterization of mesenchymal stem cells derived from equine adipose tissueCaracterização das células tronco mesenquimais derivadas do tecido adiposo equinocaracterização imunofenotípicadiferenciaçãoequinotecido adiposocélula tronco mesenquimalimmunophenotypic characterizationdifferentiationequineadipose tissuemesenchymal stem cellStem cell therapy has shown promising results in tendinitis and osteoarthritis in equine medicine. The purpose of this work was to characterize the adipose-derived mesenchymal stem cells (AdMSCs) in horses through (1) the assessment of the capacity of progenitor cells to perform adipogenic, osteogenic and chondrogenic differentiation; and (2) flow cytometry analysis using the stemness related markers: CD44, CD90, CD105 and MHC Class II. Five mixed-breed horses, aged 2-4 years-old were used to collect adipose tissue from the base of the tail. After isolation and culture of AdMSCs, immunophenotypic characterization was performed through flow cytometry. There was a high expression of CD44, CD90 and CD105, and no expression of MHC Class II markers. The tri-lineage differentiation was confirmed by specific staining: adipogenic (Oil Red O), osteogenic (Alizarin Red), and chondrogenic (Alcian Blue). The equine AdMSCs are a promising type of adult progenitor cell for tissue engineering in veterinary medicine.O uso de células tronco tem demonstrado resultados promissores na terapia da tendinite e osteoartrite na medicina equina. O objetivo deste trabalho foi caracterizar as células tronco mesenquimais derivadas do tecido adiposo (AdCTMs) em cavalos através da (1) avaliação da capacidade das células progenitoras para realizar a diferenciação adipogênica, osteogênica e condrogênica; e (2) através da análise por citometria de fluxo, utilizando os marcadores stemness relacionados: CD44, CD90, CD105 e MHC de Classe II. Cinco cavalos sem raça definida, de 2 a 4 anos de idade foram utilizados para a coleta do tecido adiposo da base da cauda. Após o isolamento e cultivo das AdCTMs, a caracterização imunofenotípica foi realizada pela citometria de fluxo. Houve alta expressão dos marcadores CD44, CD90 e CD105, e não houve expressão do MHC Classe II. A diferenciação foi confirmada pela coloração específica: adipogênica (Oil Red O), osteogênico (Alizarin Red), e condrogênico (Alcian Blue). As AdCTMs são um tipo promissor de células progenitoras adulta para a engenharia tecidual na medicina veterinária.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Universidade Estadual Paulista Faculdade de Medicina Veterinária e ZootecniaUniversidade Estadual Paulista Faculdade de Medicina de BotucatuUniversidade Estadual Paulista Faculdade de Medicina Veterinária e ZootecniaUniversidade Estadual Paulista Faculdade de Medicina de BotucatuFAPESP: 09/10670-8FAPESP: 10/03567-3Universidade Federal de Minas Gerais (UFMG), Escola de VeterináriaUniversidade Estadual Paulista (Unesp)Carvalho, A.m. [UNESP]Yamada, A.l.m. [UNESP]Golim, M.a. [UNESP]Álvarez, L.e.c. [UNESP]Jorge, L.l. [UNESP]Conceição, M.l. [UNESP]Deffune, Elenice [UNESP]Hussni, C.a. [UNESP]Alves, A.l.g. [UNESP]2014-10-01T13:08:38Z2014-10-01T13:08:38Z2013-08-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article939-945application/pdfhttp://dx.doi.org/10.1590/S0102-09352013000400001Arquivo Brasileiro de Medicina Veterinária e Zootecnia. Universidade Federal de Minas Gerais, Escola de Veterinária, v. 65, n. 4, p. 939-945, 2013.0102-0935http://hdl.handle.net/11449/10986710.1590/S0102-09352013000400001S0102-09352013000400001WOS:000324292900001S0102-09352013000400001.pdf9646764071339214SciELOreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengArquivo Brasileiro de Medicina Veterinária e Zootecnia0.2860,248info:eu-repo/semantics/openAccess2024-09-03T14:29:57Zoai:repositorio.unesp.br:11449/109867Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestrepositoriounesp@unesp.bropendoar:29462024-09-03T14:29:57Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Characterization of mesenchymal stem cells derived from equine adipose tissue Caracterização das células tronco mesenquimais derivadas do tecido adiposo equino |
title |
Characterization of mesenchymal stem cells derived from equine adipose tissue |
spellingShingle |
Characterization of mesenchymal stem cells derived from equine adipose tissue Carvalho, A.m. [UNESP] caracterização imunofenotípica diferenciação equino tecido adiposo célula tronco mesenquimal immunophenotypic characterization differentiation equine adipose tissue mesenchymal stem cell |
title_short |
Characterization of mesenchymal stem cells derived from equine adipose tissue |
title_full |
Characterization of mesenchymal stem cells derived from equine adipose tissue |
title_fullStr |
Characterization of mesenchymal stem cells derived from equine adipose tissue |
title_full_unstemmed |
Characterization of mesenchymal stem cells derived from equine adipose tissue |
title_sort |
Characterization of mesenchymal stem cells derived from equine adipose tissue |
author |
Carvalho, A.m. [UNESP] |
author_facet |
Carvalho, A.m. [UNESP] Yamada, A.l.m. [UNESP] Golim, M.a. [UNESP] Álvarez, L.e.c. [UNESP] Jorge, L.l. [UNESP] Conceição, M.l. [UNESP] Deffune, Elenice [UNESP] Hussni, C.a. [UNESP] Alves, A.l.g. [UNESP] |
author_role |
author |
author2 |
Yamada, A.l.m. [UNESP] Golim, M.a. [UNESP] Álvarez, L.e.c. [UNESP] Jorge, L.l. [UNESP] Conceição, M.l. [UNESP] Deffune, Elenice [UNESP] Hussni, C.a. [UNESP] Alves, A.l.g. [UNESP] |
author2_role |
author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Carvalho, A.m. [UNESP] Yamada, A.l.m. [UNESP] Golim, M.a. [UNESP] Álvarez, L.e.c. [UNESP] Jorge, L.l. [UNESP] Conceição, M.l. [UNESP] Deffune, Elenice [UNESP] Hussni, C.a. [UNESP] Alves, A.l.g. [UNESP] |
dc.subject.por.fl_str_mv |
caracterização imunofenotípica diferenciação equino tecido adiposo célula tronco mesenquimal immunophenotypic characterization differentiation equine adipose tissue mesenchymal stem cell |
topic |
caracterização imunofenotípica diferenciação equino tecido adiposo célula tronco mesenquimal immunophenotypic characterization differentiation equine adipose tissue mesenchymal stem cell |
description |
Stem cell therapy has shown promising results in tendinitis and osteoarthritis in equine medicine. The purpose of this work was to characterize the adipose-derived mesenchymal stem cells (AdMSCs) in horses through (1) the assessment of the capacity of progenitor cells to perform adipogenic, osteogenic and chondrogenic differentiation; and (2) flow cytometry analysis using the stemness related markers: CD44, CD90, CD105 and MHC Class II. Five mixed-breed horses, aged 2-4 years-old were used to collect adipose tissue from the base of the tail. After isolation and culture of AdMSCs, immunophenotypic characterization was performed through flow cytometry. There was a high expression of CD44, CD90 and CD105, and no expression of MHC Class II markers. The tri-lineage differentiation was confirmed by specific staining: adipogenic (Oil Red O), osteogenic (Alizarin Red), and chondrogenic (Alcian Blue). The equine AdMSCs are a promising type of adult progenitor cell for tissue engineering in veterinary medicine. |
publishDate |
2013 |
dc.date.none.fl_str_mv |
2013-08-01 2014-10-01T13:08:38Z 2014-10-01T13:08:38Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1590/S0102-09352013000400001 Arquivo Brasileiro de Medicina Veterinária e Zootecnia. Universidade Federal de Minas Gerais, Escola de Veterinária, v. 65, n. 4, p. 939-945, 2013. 0102-0935 http://hdl.handle.net/11449/109867 10.1590/S0102-09352013000400001 S0102-09352013000400001 WOS:000324292900001 S0102-09352013000400001.pdf 9646764071339214 |
url |
http://dx.doi.org/10.1590/S0102-09352013000400001 http://hdl.handle.net/11449/109867 |
identifier_str_mv |
Arquivo Brasileiro de Medicina Veterinária e Zootecnia. Universidade Federal de Minas Gerais, Escola de Veterinária, v. 65, n. 4, p. 939-945, 2013. 0102-0935 10.1590/S0102-09352013000400001 S0102-09352013000400001 WOS:000324292900001 S0102-09352013000400001.pdf 9646764071339214 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Arquivo Brasileiro de Medicina Veterinária e Zootecnia 0.286 0,248 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
939-945 application/pdf |
dc.publisher.none.fl_str_mv |
Universidade Federal de Minas Gerais (UFMG), Escola de Veterinária |
publisher.none.fl_str_mv |
Universidade Federal de Minas Gerais (UFMG), Escola de Veterinária |
dc.source.none.fl_str_mv |
SciELO reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
repositoriounesp@unesp.br |
_version_ |
1810021369073631232 |