Distal extracellular teneurin region (teneurin C-terminal associated peptide; TCAP) possesses independent intracellular calcium regulating actions, in vitro: A potential antagonist of corticotropin-releasing factor (CRF)
Autor(a) principal: | |
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Data de Publicação: | 2022 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1016/j.bbrep.2022.101397 http://hdl.handle.net/11449/246380 |
Resumo: | Teneurin C-terminal associated peptides (TCAP) are natural bioactive peptides that possess anxiety-reducing roles in animals, in vivo, and increase cell viability, in vitro. Although these peptides have some primary structural similarity to corticotropin-releasing factor (CRF), they are derived from the distal extracellular region of the teneurin transmembrane protein where they may act as separate soluble peptides after auto-catalytic cleavage from the teneurin protein following interaction with the cognate teneurin receptor, latrophilin (ADGRL), or expressed as a separate mRNA. However, although the signal transduction mechanism of TCAP in neurons has not been established, previous studies indicate an association with the intracellular calcium flux. Therefore, in this study, we have characterized the TCAP-mediated calcium response in hypothalamic cell lines using single-cell calcium methods with pharmacological antagonists to identify potential calcium channels, in vitro. Under normal circumstances, TCAP-1 reduces cytosolic calcium concentrations by uptake into the mitochondria and efflux through the plasma membrane independently of the teneurins. In doing so, TCAP-1 could inhibit the potential ‘stress’ -inducing actions of CRF. |
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Repositório Institucional da UNESP |
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Distal extracellular teneurin region (teneurin C-terminal associated peptide; TCAP) possesses independent intracellular calcium regulating actions, in vitro: A potential antagonist of corticotropin-releasing factor (CRF)BenzamilCalciumCalcium channel blockersCorticotropin releasing factorGPCRLatrophilinMetabolismMitochondriaStressTeneurinThapsigarginVanadateTeneurin C-terminal associated peptides (TCAP) are natural bioactive peptides that possess anxiety-reducing roles in animals, in vivo, and increase cell viability, in vitro. Although these peptides have some primary structural similarity to corticotropin-releasing factor (CRF), they are derived from the distal extracellular region of the teneurin transmembrane protein where they may act as separate soluble peptides after auto-catalytic cleavage from the teneurin protein following interaction with the cognate teneurin receptor, latrophilin (ADGRL), or expressed as a separate mRNA. However, although the signal transduction mechanism of TCAP in neurons has not been established, previous studies indicate an association with the intracellular calcium flux. Therefore, in this study, we have characterized the TCAP-mediated calcium response in hypothalamic cell lines using single-cell calcium methods with pharmacological antagonists to identify potential calcium channels, in vitro. Under normal circumstances, TCAP-1 reduces cytosolic calcium concentrations by uptake into the mitochondria and efflux through the plasma membrane independently of the teneurins. In doing so, TCAP-1 could inhibit the potential ‘stress’ -inducing actions of CRF.Natural Sciences and Engineering Research Council of CanadaDepartment of Cell and Systems Biology University of TorontoSao Paulo State UniversityDepartment of Physiology University of TorontoStructural Genomics Consortium University of TorontoDepartment of Toxicology and Physiology University of TorontoSao Paulo State UniversityUniversity of TorontoUniversidade Estadual Paulista (UNESP)Hogg, David W.Casatti, Claudio C. [UNESP]Belsham, Denise D.Baršytė-Lovejoy, DaliaLovejoy, David A.2023-07-29T12:39:23Z2023-07-29T12:39:23Z2022-12-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.1016/j.bbrep.2022.101397Biochemistry and Biophysics Reports, v. 32.2405-5808http://hdl.handle.net/11449/24638010.1016/j.bbrep.2022.1013972-s2.0-85142733188Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengBiochemistry and Biophysics Reportsinfo:eu-repo/semantics/openAccess2023-07-29T12:39:23Zoai:repositorio.unesp.br:11449/246380Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T18:36:32.972454Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Distal extracellular teneurin region (teneurin C-terminal associated peptide; TCAP) possesses independent intracellular calcium regulating actions, in vitro: A potential antagonist of corticotropin-releasing factor (CRF) |
title |
Distal extracellular teneurin region (teneurin C-terminal associated peptide; TCAP) possesses independent intracellular calcium regulating actions, in vitro: A potential antagonist of corticotropin-releasing factor (CRF) |
spellingShingle |
Distal extracellular teneurin region (teneurin C-terminal associated peptide; TCAP) possesses independent intracellular calcium regulating actions, in vitro: A potential antagonist of corticotropin-releasing factor (CRF) Hogg, David W. Benzamil Calcium Calcium channel blockers Corticotropin releasing factor GPCR Latrophilin Metabolism Mitochondria Stress Teneurin Thapsigargin Vanadate |
title_short |
Distal extracellular teneurin region (teneurin C-terminal associated peptide; TCAP) possesses independent intracellular calcium regulating actions, in vitro: A potential antagonist of corticotropin-releasing factor (CRF) |
title_full |
Distal extracellular teneurin region (teneurin C-terminal associated peptide; TCAP) possesses independent intracellular calcium regulating actions, in vitro: A potential antagonist of corticotropin-releasing factor (CRF) |
title_fullStr |
Distal extracellular teneurin region (teneurin C-terminal associated peptide; TCAP) possesses independent intracellular calcium regulating actions, in vitro: A potential antagonist of corticotropin-releasing factor (CRF) |
title_full_unstemmed |
Distal extracellular teneurin region (teneurin C-terminal associated peptide; TCAP) possesses independent intracellular calcium regulating actions, in vitro: A potential antagonist of corticotropin-releasing factor (CRF) |
title_sort |
Distal extracellular teneurin region (teneurin C-terminal associated peptide; TCAP) possesses independent intracellular calcium regulating actions, in vitro: A potential antagonist of corticotropin-releasing factor (CRF) |
author |
Hogg, David W. |
author_facet |
Hogg, David W. Casatti, Claudio C. [UNESP] Belsham, Denise D. Baršytė-Lovejoy, Dalia Lovejoy, David A. |
author_role |
author |
author2 |
Casatti, Claudio C. [UNESP] Belsham, Denise D. Baršytė-Lovejoy, Dalia Lovejoy, David A. |
author2_role |
author author author author |
dc.contributor.none.fl_str_mv |
University of Toronto Universidade Estadual Paulista (UNESP) |
dc.contributor.author.fl_str_mv |
Hogg, David W. Casatti, Claudio C. [UNESP] Belsham, Denise D. Baršytė-Lovejoy, Dalia Lovejoy, David A. |
dc.subject.por.fl_str_mv |
Benzamil Calcium Calcium channel blockers Corticotropin releasing factor GPCR Latrophilin Metabolism Mitochondria Stress Teneurin Thapsigargin Vanadate |
topic |
Benzamil Calcium Calcium channel blockers Corticotropin releasing factor GPCR Latrophilin Metabolism Mitochondria Stress Teneurin Thapsigargin Vanadate |
description |
Teneurin C-terminal associated peptides (TCAP) are natural bioactive peptides that possess anxiety-reducing roles in animals, in vivo, and increase cell viability, in vitro. Although these peptides have some primary structural similarity to corticotropin-releasing factor (CRF), they are derived from the distal extracellular region of the teneurin transmembrane protein where they may act as separate soluble peptides after auto-catalytic cleavage from the teneurin protein following interaction with the cognate teneurin receptor, latrophilin (ADGRL), or expressed as a separate mRNA. However, although the signal transduction mechanism of TCAP in neurons has not been established, previous studies indicate an association with the intracellular calcium flux. Therefore, in this study, we have characterized the TCAP-mediated calcium response in hypothalamic cell lines using single-cell calcium methods with pharmacological antagonists to identify potential calcium channels, in vitro. Under normal circumstances, TCAP-1 reduces cytosolic calcium concentrations by uptake into the mitochondria and efflux through the plasma membrane independently of the teneurins. In doing so, TCAP-1 could inhibit the potential ‘stress’ -inducing actions of CRF. |
publishDate |
2022 |
dc.date.none.fl_str_mv |
2022-12-01 2023-07-29T12:39:23Z 2023-07-29T12:39:23Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1016/j.bbrep.2022.101397 Biochemistry and Biophysics Reports, v. 32. 2405-5808 http://hdl.handle.net/11449/246380 10.1016/j.bbrep.2022.101397 2-s2.0-85142733188 |
url |
http://dx.doi.org/10.1016/j.bbrep.2022.101397 http://hdl.handle.net/11449/246380 |
identifier_str_mv |
Biochemistry and Biophysics Reports, v. 32. 2405-5808 10.1016/j.bbrep.2022.101397 2-s2.0-85142733188 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Biochemistry and Biophysics Reports |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808128955488665600 |