Differences in global gene expression in muscle tissue of Nellore cattle with divergent meat tenderness
Autor(a) principal: | |
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Data de Publicação: | 2017 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1186/s12864-017-4323-0 http://hdl.handle.net/11449/179421 |
Resumo: | Background: Meat tenderness is the consumer's most preferred sensory attribute. This trait is affected by a number of factors, including genotype, age, animal sex, and pre- and post-slaughter management. In view of the high percentage of Zebu genes in the Brazilian cattle population, mainly Nellore cattle, the improvement of meat tenderness is important since the increasing proportion of Zebu genes in the population reduces meat tenderness. However, the measurement of this trait is difficult once it can only be made after animal slaughtering. New technologies such as RNA-Seq have been used to increase our understanding of the genetic processes regulating quantitative traits phenotypes. The objective of this study was to identify differentially expressed genes related to meat tenderness, in Nellore cattle in order to elucidate the genetic factors associated with meat quality. Samples were collected 24 h postmortem and the meat was not aged. Results: We found 40 differentially expressed genes related to meat tenderness, 17 with known functions. Fourteen genes were up-regulated and 3 were down-regulated in the tender meat group. Genes related to ubiquitin metabolism, transport of molecules such as calcium and oxygen, acid-base balance, collagen production, actin, myosin, and fat were identified. The PCP4L1 (Purkinje cell protein 4 like 1) and BoLA-DQB (major histocompatibility complex, class II, DQ beta) genes were validated by qRT-PCR. The results showed relative expression values similar to those obtained by RNA-Seq, with the same direction of expression (i.e., the two techniques revealed higher expression of PCP4L1 in tender meat samples and of BoLA-DQB in tough meat samples). Conclusions: This study revealed the differential expression of genes and functions in Nellore cattle muscle tissue, which may contain potential biomarkers involved in meat tenderness. |
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Differences in global gene expression in muscle tissue of Nellore cattle with divergent meat tendernessMeat qualityRNA-SeqTranscriptomeBackground: Meat tenderness is the consumer's most preferred sensory attribute. This trait is affected by a number of factors, including genotype, age, animal sex, and pre- and post-slaughter management. In view of the high percentage of Zebu genes in the Brazilian cattle population, mainly Nellore cattle, the improvement of meat tenderness is important since the increasing proportion of Zebu genes in the population reduces meat tenderness. However, the measurement of this trait is difficult once it can only be made after animal slaughtering. New technologies such as RNA-Seq have been used to increase our understanding of the genetic processes regulating quantitative traits phenotypes. The objective of this study was to identify differentially expressed genes related to meat tenderness, in Nellore cattle in order to elucidate the genetic factors associated with meat quality. Samples were collected 24 h postmortem and the meat was not aged. Results: We found 40 differentially expressed genes related to meat tenderness, 17 with known functions. Fourteen genes were up-regulated and 3 were down-regulated in the tender meat group. Genes related to ubiquitin metabolism, transport of molecules such as calcium and oxygen, acid-base balance, collagen production, actin, myosin, and fat were identified. The PCP4L1 (Purkinje cell protein 4 like 1) and BoLA-DQB (major histocompatibility complex, class II, DQ beta) genes were validated by qRT-PCR. The results showed relative expression values similar to those obtained by RNA-Seq, with the same direction of expression (i.e., the two techniques revealed higher expression of PCP4L1 in tender meat samples and of BoLA-DQB in tough meat samples). Conclusions: This study revealed the differential expression of genes and functions in Nellore cattle muscle tissue, which may contain potential biomarkers involved in meat tenderness.São Paulo State University FCAV/UNESP Faculty of Agricultural and Veterinary SciencesCyVerse University of ArizonaSão Paulo State University FCAV/UNESP Faculty of Agricultural and Veterinary SciencesUniversidade Estadual Paulista (Unesp)University of ArizonaFonseca, Larissa Fernanda Simielli [UNESP]Gimenez, Daniele Fernanda Jovino [UNESP]dos Santos Silva, Danielly Beraldo [UNESP]Barthelson, RogerBaldi, Fernando [UNESP]Ferro, Jesus Aparecido [UNESP]Albuquerque, Lucia Galvão [UNESP]2018-12-11T17:35:07Z2018-12-11T17:35:07Z2017-12-04info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://dx.doi.org/10.1186/s12864-017-4323-0BMC Genomics, v. 18, n. 1, 2017.1471-2164http://hdl.handle.net/11449/17942110.1186/s12864-017-4323-02-s2.0-850373775092-s2.0-85037377509.pdf5866981114947883Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengBMC Genomics2,110info:eu-repo/semantics/openAccess2024-06-07T18:44:43Zoai:repositorio.unesp.br:11449/179421Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T23:14:20.933733Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Differences in global gene expression in muscle tissue of Nellore cattle with divergent meat tenderness |
title |
Differences in global gene expression in muscle tissue of Nellore cattle with divergent meat tenderness |
spellingShingle |
Differences in global gene expression in muscle tissue of Nellore cattle with divergent meat tenderness Fonseca, Larissa Fernanda Simielli [UNESP] Meat quality RNA-Seq Transcriptome |
title_short |
Differences in global gene expression in muscle tissue of Nellore cattle with divergent meat tenderness |
title_full |
Differences in global gene expression in muscle tissue of Nellore cattle with divergent meat tenderness |
title_fullStr |
Differences in global gene expression in muscle tissue of Nellore cattle with divergent meat tenderness |
title_full_unstemmed |
Differences in global gene expression in muscle tissue of Nellore cattle with divergent meat tenderness |
title_sort |
Differences in global gene expression in muscle tissue of Nellore cattle with divergent meat tenderness |
author |
Fonseca, Larissa Fernanda Simielli [UNESP] |
author_facet |
Fonseca, Larissa Fernanda Simielli [UNESP] Gimenez, Daniele Fernanda Jovino [UNESP] dos Santos Silva, Danielly Beraldo [UNESP] Barthelson, Roger Baldi, Fernando [UNESP] Ferro, Jesus Aparecido [UNESP] Albuquerque, Lucia Galvão [UNESP] |
author_role |
author |
author2 |
Gimenez, Daniele Fernanda Jovino [UNESP] dos Santos Silva, Danielly Beraldo [UNESP] Barthelson, Roger Baldi, Fernando [UNESP] Ferro, Jesus Aparecido [UNESP] Albuquerque, Lucia Galvão [UNESP] |
author2_role |
author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) University of Arizona |
dc.contributor.author.fl_str_mv |
Fonseca, Larissa Fernanda Simielli [UNESP] Gimenez, Daniele Fernanda Jovino [UNESP] dos Santos Silva, Danielly Beraldo [UNESP] Barthelson, Roger Baldi, Fernando [UNESP] Ferro, Jesus Aparecido [UNESP] Albuquerque, Lucia Galvão [UNESP] |
dc.subject.por.fl_str_mv |
Meat quality RNA-Seq Transcriptome |
topic |
Meat quality RNA-Seq Transcriptome |
description |
Background: Meat tenderness is the consumer's most preferred sensory attribute. This trait is affected by a number of factors, including genotype, age, animal sex, and pre- and post-slaughter management. In view of the high percentage of Zebu genes in the Brazilian cattle population, mainly Nellore cattle, the improvement of meat tenderness is important since the increasing proportion of Zebu genes in the population reduces meat tenderness. However, the measurement of this trait is difficult once it can only be made after animal slaughtering. New technologies such as RNA-Seq have been used to increase our understanding of the genetic processes regulating quantitative traits phenotypes. The objective of this study was to identify differentially expressed genes related to meat tenderness, in Nellore cattle in order to elucidate the genetic factors associated with meat quality. Samples were collected 24 h postmortem and the meat was not aged. Results: We found 40 differentially expressed genes related to meat tenderness, 17 with known functions. Fourteen genes were up-regulated and 3 were down-regulated in the tender meat group. Genes related to ubiquitin metabolism, transport of molecules such as calcium and oxygen, acid-base balance, collagen production, actin, myosin, and fat were identified. The PCP4L1 (Purkinje cell protein 4 like 1) and BoLA-DQB (major histocompatibility complex, class II, DQ beta) genes were validated by qRT-PCR. The results showed relative expression values similar to those obtained by RNA-Seq, with the same direction of expression (i.e., the two techniques revealed higher expression of PCP4L1 in tender meat samples and of BoLA-DQB in tough meat samples). Conclusions: This study revealed the differential expression of genes and functions in Nellore cattle muscle tissue, which may contain potential biomarkers involved in meat tenderness. |
publishDate |
2017 |
dc.date.none.fl_str_mv |
2017-12-04 2018-12-11T17:35:07Z 2018-12-11T17:35:07Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1186/s12864-017-4323-0 BMC Genomics, v. 18, n. 1, 2017. 1471-2164 http://hdl.handle.net/11449/179421 10.1186/s12864-017-4323-0 2-s2.0-85037377509 2-s2.0-85037377509.pdf 5866981114947883 |
url |
http://dx.doi.org/10.1186/s12864-017-4323-0 http://hdl.handle.net/11449/179421 |
identifier_str_mv |
BMC Genomics, v. 18, n. 1, 2017. 1471-2164 10.1186/s12864-017-4323-0 2-s2.0-85037377509 2-s2.0-85037377509.pdf 5866981114947883 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
BMC Genomics 2,110 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
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1808129501794664448 |