Differences in global gene expression in muscle tissue of Nellore cattle with divergent meat tenderness

Detalhes bibliográficos
Autor(a) principal: Fonseca, Larissa Fernanda Simielli [UNESP]
Data de Publicação: 2017
Outros Autores: Gimenez, Daniele Fernanda Jovino [UNESP], dos Santos Silva, Danielly Beraldo [UNESP], Barthelson, Roger, Baldi, Fernando [UNESP], Ferro, Jesus Aparecido [UNESP], Albuquerque, Lucia Galvão [UNESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1186/s12864-017-4323-0
http://hdl.handle.net/11449/179421
Resumo: Background: Meat tenderness is the consumer's most preferred sensory attribute. This trait is affected by a number of factors, including genotype, age, animal sex, and pre- and post-slaughter management. In view of the high percentage of Zebu genes in the Brazilian cattle population, mainly Nellore cattle, the improvement of meat tenderness is important since the increasing proportion of Zebu genes in the population reduces meat tenderness. However, the measurement of this trait is difficult once it can only be made after animal slaughtering. New technologies such as RNA-Seq have been used to increase our understanding of the genetic processes regulating quantitative traits phenotypes. The objective of this study was to identify differentially expressed genes related to meat tenderness, in Nellore cattle in order to elucidate the genetic factors associated with meat quality. Samples were collected 24 h postmortem and the meat was not aged. Results: We found 40 differentially expressed genes related to meat tenderness, 17 with known functions. Fourteen genes were up-regulated and 3 were down-regulated in the tender meat group. Genes related to ubiquitin metabolism, transport of molecules such as calcium and oxygen, acid-base balance, collagen production, actin, myosin, and fat were identified. The PCP4L1 (Purkinje cell protein 4 like 1) and BoLA-DQB (major histocompatibility complex, class II, DQ beta) genes were validated by qRT-PCR. The results showed relative expression values similar to those obtained by RNA-Seq, with the same direction of expression (i.e., the two techniques revealed higher expression of PCP4L1 in tender meat samples and of BoLA-DQB in tough meat samples). Conclusions: This study revealed the differential expression of genes and functions in Nellore cattle muscle tissue, which may contain potential biomarkers involved in meat tenderness.
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spelling Differences in global gene expression in muscle tissue of Nellore cattle with divergent meat tendernessMeat qualityRNA-SeqTranscriptomeBackground: Meat tenderness is the consumer's most preferred sensory attribute. This trait is affected by a number of factors, including genotype, age, animal sex, and pre- and post-slaughter management. In view of the high percentage of Zebu genes in the Brazilian cattle population, mainly Nellore cattle, the improvement of meat tenderness is important since the increasing proportion of Zebu genes in the population reduces meat tenderness. However, the measurement of this trait is difficult once it can only be made after animal slaughtering. New technologies such as RNA-Seq have been used to increase our understanding of the genetic processes regulating quantitative traits phenotypes. The objective of this study was to identify differentially expressed genes related to meat tenderness, in Nellore cattle in order to elucidate the genetic factors associated with meat quality. Samples were collected 24 h postmortem and the meat was not aged. Results: We found 40 differentially expressed genes related to meat tenderness, 17 with known functions. Fourteen genes were up-regulated and 3 were down-regulated in the tender meat group. Genes related to ubiquitin metabolism, transport of molecules such as calcium and oxygen, acid-base balance, collagen production, actin, myosin, and fat were identified. The PCP4L1 (Purkinje cell protein 4 like 1) and BoLA-DQB (major histocompatibility complex, class II, DQ beta) genes were validated by qRT-PCR. The results showed relative expression values similar to those obtained by RNA-Seq, with the same direction of expression (i.e., the two techniques revealed higher expression of PCP4L1 in tender meat samples and of BoLA-DQB in tough meat samples). Conclusions: This study revealed the differential expression of genes and functions in Nellore cattle muscle tissue, which may contain potential biomarkers involved in meat tenderness.São Paulo State University FCAV/UNESP Faculty of Agricultural and Veterinary SciencesCyVerse University of ArizonaSão Paulo State University FCAV/UNESP Faculty of Agricultural and Veterinary SciencesUniversidade Estadual Paulista (Unesp)University of ArizonaFonseca, Larissa Fernanda Simielli [UNESP]Gimenez, Daniele Fernanda Jovino [UNESP]dos Santos Silva, Danielly Beraldo [UNESP]Barthelson, RogerBaldi, Fernando [UNESP]Ferro, Jesus Aparecido [UNESP]Albuquerque, Lucia Galvão [UNESP]2018-12-11T17:35:07Z2018-12-11T17:35:07Z2017-12-04info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://dx.doi.org/10.1186/s12864-017-4323-0BMC Genomics, v. 18, n. 1, 2017.1471-2164http://hdl.handle.net/11449/17942110.1186/s12864-017-4323-02-s2.0-850373775092-s2.0-85037377509.pdf5866981114947883Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengBMC Genomics2,110info:eu-repo/semantics/openAccess2024-06-07T18:44:43Zoai:repositorio.unesp.br:11449/179421Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T23:14:20.933733Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Differences in global gene expression in muscle tissue of Nellore cattle with divergent meat tenderness
title Differences in global gene expression in muscle tissue of Nellore cattle with divergent meat tenderness
spellingShingle Differences in global gene expression in muscle tissue of Nellore cattle with divergent meat tenderness
Fonseca, Larissa Fernanda Simielli [UNESP]
Meat quality
RNA-Seq
Transcriptome
title_short Differences in global gene expression in muscle tissue of Nellore cattle with divergent meat tenderness
title_full Differences in global gene expression in muscle tissue of Nellore cattle with divergent meat tenderness
title_fullStr Differences in global gene expression in muscle tissue of Nellore cattle with divergent meat tenderness
title_full_unstemmed Differences in global gene expression in muscle tissue of Nellore cattle with divergent meat tenderness
title_sort Differences in global gene expression in muscle tissue of Nellore cattle with divergent meat tenderness
author Fonseca, Larissa Fernanda Simielli [UNESP]
author_facet Fonseca, Larissa Fernanda Simielli [UNESP]
Gimenez, Daniele Fernanda Jovino [UNESP]
dos Santos Silva, Danielly Beraldo [UNESP]
Barthelson, Roger
Baldi, Fernando [UNESP]
Ferro, Jesus Aparecido [UNESP]
Albuquerque, Lucia Galvão [UNESP]
author_role author
author2 Gimenez, Daniele Fernanda Jovino [UNESP]
dos Santos Silva, Danielly Beraldo [UNESP]
Barthelson, Roger
Baldi, Fernando [UNESP]
Ferro, Jesus Aparecido [UNESP]
Albuquerque, Lucia Galvão [UNESP]
author2_role author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade Estadual Paulista (Unesp)
University of Arizona
dc.contributor.author.fl_str_mv Fonseca, Larissa Fernanda Simielli [UNESP]
Gimenez, Daniele Fernanda Jovino [UNESP]
dos Santos Silva, Danielly Beraldo [UNESP]
Barthelson, Roger
Baldi, Fernando [UNESP]
Ferro, Jesus Aparecido [UNESP]
Albuquerque, Lucia Galvão [UNESP]
dc.subject.por.fl_str_mv Meat quality
RNA-Seq
Transcriptome
topic Meat quality
RNA-Seq
Transcriptome
description Background: Meat tenderness is the consumer's most preferred sensory attribute. This trait is affected by a number of factors, including genotype, age, animal sex, and pre- and post-slaughter management. In view of the high percentage of Zebu genes in the Brazilian cattle population, mainly Nellore cattle, the improvement of meat tenderness is important since the increasing proportion of Zebu genes in the population reduces meat tenderness. However, the measurement of this trait is difficult once it can only be made after animal slaughtering. New technologies such as RNA-Seq have been used to increase our understanding of the genetic processes regulating quantitative traits phenotypes. The objective of this study was to identify differentially expressed genes related to meat tenderness, in Nellore cattle in order to elucidate the genetic factors associated with meat quality. Samples were collected 24 h postmortem and the meat was not aged. Results: We found 40 differentially expressed genes related to meat tenderness, 17 with known functions. Fourteen genes were up-regulated and 3 were down-regulated in the tender meat group. Genes related to ubiquitin metabolism, transport of molecules such as calcium and oxygen, acid-base balance, collagen production, actin, myosin, and fat were identified. The PCP4L1 (Purkinje cell protein 4 like 1) and BoLA-DQB (major histocompatibility complex, class II, DQ beta) genes were validated by qRT-PCR. The results showed relative expression values similar to those obtained by RNA-Seq, with the same direction of expression (i.e., the two techniques revealed higher expression of PCP4L1 in tender meat samples and of BoLA-DQB in tough meat samples). Conclusions: This study revealed the differential expression of genes and functions in Nellore cattle muscle tissue, which may contain potential biomarkers involved in meat tenderness.
publishDate 2017
dc.date.none.fl_str_mv 2017-12-04
2018-12-11T17:35:07Z
2018-12-11T17:35:07Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1186/s12864-017-4323-0
BMC Genomics, v. 18, n. 1, 2017.
1471-2164
http://hdl.handle.net/11449/179421
10.1186/s12864-017-4323-0
2-s2.0-85037377509
2-s2.0-85037377509.pdf
5866981114947883
url http://dx.doi.org/10.1186/s12864-017-4323-0
http://hdl.handle.net/11449/179421
identifier_str_mv BMC Genomics, v. 18, n. 1, 2017.
1471-2164
10.1186/s12864-017-4323-0
2-s2.0-85037377509
2-s2.0-85037377509.pdf
5866981114947883
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv BMC Genomics
2,110
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
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