Bioactive paper platform for detection of hydrogen peroxide in milk
Autor(a) principal: | |
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Data de Publicação: | 2020 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1016/j.saa.2019.117774 http://hdl.handle.net/11449/201351 |
Resumo: | This paper describes the development and application of a paper-based analytical device (μPAD) for the determination of hydrogen peroxide, an important adulterant in milk. The method employs the reaction between hydrogen peroxide and guaiacol, catalyzed by peroxidase, producing a red product, which is then quantified by digital imaging. Experimental design methodology was used to optimize the experimental conditions. The linear concentration range was from 12.5 × 10− 4 to 150 × 10− 4 mol L− 1, resulting in the regression equation AB = 0.02466 (±0.00192) + 17.053 (±0.750) C, with an excellent correlation coefficient (r = 0.986). The relative standard deviations obtained were 1.1 and 1.3% (intra-day), and 4.8 and 2.9% (inter-day), for 25.0 × 10−4 and 100 × 10−4 mol L−1 of hydrogen peroxide, respectively. The limits of detection and quantification were 3.54 × 10− 4 and 11.8 × 10− 4 mol L− 1, respectively, with standard deviation of the blank of 0.002012. The proposed method was successfully applied for the determination of peroxide in milk samples, with recoveries between 92.2 and 109%. The proposed device constitutes a valuable analytical tool for the identification of hydrogen peroxide adulteration and offers advantages including low cost, simplicity, portability, and no (or minimal) requirement for sample pretreatment. |
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Bioactive paper platform for detection of hydrogen peroxide in milkBioactive paper platformDigital imageHydrogen peroxideMilk adulterationThis paper describes the development and application of a paper-based analytical device (μPAD) for the determination of hydrogen peroxide, an important adulterant in milk. The method employs the reaction between hydrogen peroxide and guaiacol, catalyzed by peroxidase, producing a red product, which is then quantified by digital imaging. Experimental design methodology was used to optimize the experimental conditions. The linear concentration range was from 12.5 × 10− 4 to 150 × 10− 4 mol L− 1, resulting in the regression equation AB = 0.02466 (±0.00192) + 17.053 (±0.750) C, with an excellent correlation coefficient (r = 0.986). The relative standard deviations obtained were 1.1 and 1.3% (intra-day), and 4.8 and 2.9% (inter-day), for 25.0 × 10−4 and 100 × 10−4 mol L−1 of hydrogen peroxide, respectively. The limits of detection and quantification were 3.54 × 10− 4 and 11.8 × 10− 4 mol L− 1, respectively, with standard deviation of the blank of 0.002012. The proposed method was successfully applied for the determination of peroxide in milk samples, with recoveries between 92.2 and 109%. The proposed device constitutes a valuable analytical tool for the identification of hydrogen peroxide adulteration and offers advantages including low cost, simplicity, portability, and no (or minimal) requirement for sample pretreatment.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Instituto de Química UNESP – São Paulo State University, R. Prof. Francisco Degni 55, P.O.Box 355Instituto de Química UNESP – São Paulo State University, R. Prof. Francisco Degni 55, P.O.Box 355FAPESP: #2015/21733-1CAPES: 001Universidade Estadual Paulista (Unesp)Lima, Liliane Spazzapam [UNESP]Rossini, Eduardo Luiz [UNESP]Pezza, Leonardo [UNESP]Pezza, Helena Redigolo [UNESP]2020-12-12T02:30:20Z2020-12-12T02:30:20Z2020-02-15info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.1016/j.saa.2019.117774Spectrochimica Acta - Part A: Molecular and Biomolecular Spectroscopy, v. 227.1386-1425http://hdl.handle.net/11449/20135110.1016/j.saa.2019.1177742-s2.0-85075523801Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengSpectrochimica Acta - Part A: Molecular and Biomolecular Spectroscopyinfo:eu-repo/semantics/openAccess2021-10-22T17:27:28Zoai:repositorio.unesp.br:11449/201351Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T20:49:03.295596Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Bioactive paper platform for detection of hydrogen peroxide in milk |
title |
Bioactive paper platform for detection of hydrogen peroxide in milk |
spellingShingle |
Bioactive paper platform for detection of hydrogen peroxide in milk Lima, Liliane Spazzapam [UNESP] Bioactive paper platform Digital image Hydrogen peroxide Milk adulteration |
title_short |
Bioactive paper platform for detection of hydrogen peroxide in milk |
title_full |
Bioactive paper platform for detection of hydrogen peroxide in milk |
title_fullStr |
Bioactive paper platform for detection of hydrogen peroxide in milk |
title_full_unstemmed |
Bioactive paper platform for detection of hydrogen peroxide in milk |
title_sort |
Bioactive paper platform for detection of hydrogen peroxide in milk |
author |
Lima, Liliane Spazzapam [UNESP] |
author_facet |
Lima, Liliane Spazzapam [UNESP] Rossini, Eduardo Luiz [UNESP] Pezza, Leonardo [UNESP] Pezza, Helena Redigolo [UNESP] |
author_role |
author |
author2 |
Rossini, Eduardo Luiz [UNESP] Pezza, Leonardo [UNESP] Pezza, Helena Redigolo [UNESP] |
author2_role |
author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Lima, Liliane Spazzapam [UNESP] Rossini, Eduardo Luiz [UNESP] Pezza, Leonardo [UNESP] Pezza, Helena Redigolo [UNESP] |
dc.subject.por.fl_str_mv |
Bioactive paper platform Digital image Hydrogen peroxide Milk adulteration |
topic |
Bioactive paper platform Digital image Hydrogen peroxide Milk adulteration |
description |
This paper describes the development and application of a paper-based analytical device (μPAD) for the determination of hydrogen peroxide, an important adulterant in milk. The method employs the reaction between hydrogen peroxide and guaiacol, catalyzed by peroxidase, producing a red product, which is then quantified by digital imaging. Experimental design methodology was used to optimize the experimental conditions. The linear concentration range was from 12.5 × 10− 4 to 150 × 10− 4 mol L− 1, resulting in the regression equation AB = 0.02466 (±0.00192) + 17.053 (±0.750) C, with an excellent correlation coefficient (r = 0.986). The relative standard deviations obtained were 1.1 and 1.3% (intra-day), and 4.8 and 2.9% (inter-day), for 25.0 × 10−4 and 100 × 10−4 mol L−1 of hydrogen peroxide, respectively. The limits of detection and quantification were 3.54 × 10− 4 and 11.8 × 10− 4 mol L− 1, respectively, with standard deviation of the blank of 0.002012. The proposed method was successfully applied for the determination of peroxide in milk samples, with recoveries between 92.2 and 109%. The proposed device constitutes a valuable analytical tool for the identification of hydrogen peroxide adulteration and offers advantages including low cost, simplicity, portability, and no (or minimal) requirement for sample pretreatment. |
publishDate |
2020 |
dc.date.none.fl_str_mv |
2020-12-12T02:30:20Z 2020-12-12T02:30:20Z 2020-02-15 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1016/j.saa.2019.117774 Spectrochimica Acta - Part A: Molecular and Biomolecular Spectroscopy, v. 227. 1386-1425 http://hdl.handle.net/11449/201351 10.1016/j.saa.2019.117774 2-s2.0-85075523801 |
url |
http://dx.doi.org/10.1016/j.saa.2019.117774 http://hdl.handle.net/11449/201351 |
identifier_str_mv |
Spectrochimica Acta - Part A: Molecular and Biomolecular Spectroscopy, v. 227. 1386-1425 10.1016/j.saa.2019.117774 2-s2.0-85075523801 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Spectrochimica Acta - Part A: Molecular and Biomolecular Spectroscopy |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808129252057415680 |