The effects of mercury exposure on Amazonian fishes: An investigation of potential biomarkers
Autor(a) principal: | |
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Data de Publicação: | 2023 |
Outros Autores: | , , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1016/j.chemosphere.2023.137779 http://hdl.handle.net/11449/249549 |
Resumo: | Exposure to mercury can interfere with the expression of proteins and enzymes, compromise important pathways, such as apoptosis and glucose metabolism, and even induce the expression of metallothioneins. In this study, analytical techniques were used to determine the concentration of total mercury (THg) in muscle and liver tissue, protein pellets, and spots [using graphite furnace atomic absorption spectrometry (GFAAS)], and molecular techniques were used to identify metalloproteins present in mercury-associated protein spots. Thirty individuals from three different fish species, Cichla sp. (n = 10), Brachyplatystoma filamentosum (n = 10), and Semaprochilodus sp. (n = 10) from the Brazilian Amazon were used. Oxidative stress indicators [such as glutathione peroxidase (GSH-Px), catalase (CAT), superoxide dismutase (SOD), a marker of lipid peroxidation (LPO)] and the possible expression of metallothioneins in muscle and liver tissues were investigated. The two piscivorous species, Cichla sp. and B. filamentosum, presented the highest concentrations of mercury in their hepatic tissue, 1219 ± 15.00 and 1044 ± 13.6 μg kg−1, respectively, and in their muscle tissue, 101 ± 1.30 μg kg−1 and 87.4 ± 0.900 μg kg−1, respectively. The non-carnivorous species Semaprochilodus sp. had comparatively low concentrations of mercury in both its hepatic (852 ± 11.1 μg kg−1) and muscle (71.4 ± 0.930 μg kg−1) tissues. The presence of mercury was identified in 24 protein spots using GFAAS; concentrations ranged from 11.5 to 787 μg kg−1, and mass spectrometry identified 21 metal-binding proteins. The activities of GSH-Px, CAT, and SOD, related to oxidative stress, decreased proportionally as tissue Hg concentrations increased, while the levels of LPO markers increased, indicating the presence of stress. Our study results demonstrate possible mercury interference in oxidative stress markers (GSH-Px, CAT, SOD, and LPO), in addition to the identification of 21 metal-binding proteins as possible biomarkers of mercury exposure in fish. |
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The effects of mercury exposure on Amazonian fishes: An investigation of potential biomarkersMercury biomarkersMetal-binding proteinMetalloproteomicsMetallothioneinExposure to mercury can interfere with the expression of proteins and enzymes, compromise important pathways, such as apoptosis and glucose metabolism, and even induce the expression of metallothioneins. In this study, analytical techniques were used to determine the concentration of total mercury (THg) in muscle and liver tissue, protein pellets, and spots [using graphite furnace atomic absorption spectrometry (GFAAS)], and molecular techniques were used to identify metalloproteins present in mercury-associated protein spots. Thirty individuals from three different fish species, Cichla sp. (n = 10), Brachyplatystoma filamentosum (n = 10), and Semaprochilodus sp. (n = 10) from the Brazilian Amazon were used. Oxidative stress indicators [such as glutathione peroxidase (GSH-Px), catalase (CAT), superoxide dismutase (SOD), a marker of lipid peroxidation (LPO)] and the possible expression of metallothioneins in muscle and liver tissues were investigated. The two piscivorous species, Cichla sp. and B. filamentosum, presented the highest concentrations of mercury in their hepatic tissue, 1219 ± 15.00 and 1044 ± 13.6 μg kg−1, respectively, and in their muscle tissue, 101 ± 1.30 μg kg−1 and 87.4 ± 0.900 μg kg−1, respectively. The non-carnivorous species Semaprochilodus sp. had comparatively low concentrations of mercury in both its hepatic (852 ± 11.1 μg kg−1) and muscle (71.4 ± 0.930 μg kg−1) tissues. The presence of mercury was identified in 24 protein spots using GFAAS; concentrations ranged from 11.5 to 787 μg kg−1, and mass spectrometry identified 21 metal-binding proteins. The activities of GSH-Px, CAT, and SOD, related to oxidative stress, decreased proportionally as tissue Hg concentrations increased, while the levels of LPO markers increased, indicating the presence of stress. Our study results demonstrate possible mercury interference in oxidative stress markers (GSH-Px, CAT, SOD, and LPO), in addition to the identification of 21 metal-binding proteins as possible biomarkers of mercury exposure in fish.São Paulo State University (UNESP) Institute of Biosciences, São PauloUniversity of Nebraska (UNL)São Paulo State University (UNESP) School of Veterinary Medicine and Animal Science, SPUniversity of São Paulo (USP)University of Brasília (UNB) College of PlanaltinaSão Paulo State University (UNESP) Institute of Biosciences, São PauloSão Paulo State University (UNESP) School of Veterinary Medicine and Animal Science, SPUniversidade Estadual Paulista (UNESP)University of Nebraska (UNL)Universidade de São Paulo (USP)College of PlanaltinaVieira, José Cavalcante Souza [UNESP]Braga, Camila PereiraQueiroz, João Vitor de [UNESP]Cavecci-Mendonça, Bruna [UNESP]Oliveira, Grasieli de [UNESP]Freitas, Natália Galvão de [UNESP]Fernandes, Ana Angélica Henrique [UNESP]Fernandes, Mileni da SilvaBuzalaf, Marília Afonso RabeloAdamec, JiriZara, Luiz FabrícioPadilha, Pedro de Magalhães [UNESP]2023-07-29T16:02:50Z2023-07-29T16:02:50Z2023-03-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.1016/j.chemosphere.2023.137779Chemosphere, v. 316.1879-12980045-6535http://hdl.handle.net/11449/24954910.1016/j.chemosphere.2023.1377792-s2.0-85146161032Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengChemosphereinfo:eu-repo/semantics/openAccess2023-07-29T16:02:50Zoai:repositorio.unesp.br:11449/249549Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462023-07-29T16:02:50Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
The effects of mercury exposure on Amazonian fishes: An investigation of potential biomarkers |
title |
The effects of mercury exposure on Amazonian fishes: An investigation of potential biomarkers |
spellingShingle |
The effects of mercury exposure on Amazonian fishes: An investigation of potential biomarkers Vieira, José Cavalcante Souza [UNESP] Mercury biomarkers Metal-binding protein Metalloproteomics Metallothionein |
title_short |
The effects of mercury exposure on Amazonian fishes: An investigation of potential biomarkers |
title_full |
The effects of mercury exposure on Amazonian fishes: An investigation of potential biomarkers |
title_fullStr |
The effects of mercury exposure on Amazonian fishes: An investigation of potential biomarkers |
title_full_unstemmed |
The effects of mercury exposure on Amazonian fishes: An investigation of potential biomarkers |
title_sort |
The effects of mercury exposure on Amazonian fishes: An investigation of potential biomarkers |
author |
Vieira, José Cavalcante Souza [UNESP] |
author_facet |
Vieira, José Cavalcante Souza [UNESP] Braga, Camila Pereira Queiroz, João Vitor de [UNESP] Cavecci-Mendonça, Bruna [UNESP] Oliveira, Grasieli de [UNESP] Freitas, Natália Galvão de [UNESP] Fernandes, Ana Angélica Henrique [UNESP] Fernandes, Mileni da Silva Buzalaf, Marília Afonso Rabelo Adamec, Jiri Zara, Luiz Fabrício Padilha, Pedro de Magalhães [UNESP] |
author_role |
author |
author2 |
Braga, Camila Pereira Queiroz, João Vitor de [UNESP] Cavecci-Mendonça, Bruna [UNESP] Oliveira, Grasieli de [UNESP] Freitas, Natália Galvão de [UNESP] Fernandes, Ana Angélica Henrique [UNESP] Fernandes, Mileni da Silva Buzalaf, Marília Afonso Rabelo Adamec, Jiri Zara, Luiz Fabrício Padilha, Pedro de Magalhães [UNESP] |
author2_role |
author author author author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (UNESP) University of Nebraska (UNL) Universidade de São Paulo (USP) College of Planaltina |
dc.contributor.author.fl_str_mv |
Vieira, José Cavalcante Souza [UNESP] Braga, Camila Pereira Queiroz, João Vitor de [UNESP] Cavecci-Mendonça, Bruna [UNESP] Oliveira, Grasieli de [UNESP] Freitas, Natália Galvão de [UNESP] Fernandes, Ana Angélica Henrique [UNESP] Fernandes, Mileni da Silva Buzalaf, Marília Afonso Rabelo Adamec, Jiri Zara, Luiz Fabrício Padilha, Pedro de Magalhães [UNESP] |
dc.subject.por.fl_str_mv |
Mercury biomarkers Metal-binding protein Metalloproteomics Metallothionein |
topic |
Mercury biomarkers Metal-binding protein Metalloproteomics Metallothionein |
description |
Exposure to mercury can interfere with the expression of proteins and enzymes, compromise important pathways, such as apoptosis and glucose metabolism, and even induce the expression of metallothioneins. In this study, analytical techniques were used to determine the concentration of total mercury (THg) in muscle and liver tissue, protein pellets, and spots [using graphite furnace atomic absorption spectrometry (GFAAS)], and molecular techniques were used to identify metalloproteins present in mercury-associated protein spots. Thirty individuals from three different fish species, Cichla sp. (n = 10), Brachyplatystoma filamentosum (n = 10), and Semaprochilodus sp. (n = 10) from the Brazilian Amazon were used. Oxidative stress indicators [such as glutathione peroxidase (GSH-Px), catalase (CAT), superoxide dismutase (SOD), a marker of lipid peroxidation (LPO)] and the possible expression of metallothioneins in muscle and liver tissues were investigated. The two piscivorous species, Cichla sp. and B. filamentosum, presented the highest concentrations of mercury in their hepatic tissue, 1219 ± 15.00 and 1044 ± 13.6 μg kg−1, respectively, and in their muscle tissue, 101 ± 1.30 μg kg−1 and 87.4 ± 0.900 μg kg−1, respectively. The non-carnivorous species Semaprochilodus sp. had comparatively low concentrations of mercury in both its hepatic (852 ± 11.1 μg kg−1) and muscle (71.4 ± 0.930 μg kg−1) tissues. The presence of mercury was identified in 24 protein spots using GFAAS; concentrations ranged from 11.5 to 787 μg kg−1, and mass spectrometry identified 21 metal-binding proteins. The activities of GSH-Px, CAT, and SOD, related to oxidative stress, decreased proportionally as tissue Hg concentrations increased, while the levels of LPO markers increased, indicating the presence of stress. Our study results demonstrate possible mercury interference in oxidative stress markers (GSH-Px, CAT, SOD, and LPO), in addition to the identification of 21 metal-binding proteins as possible biomarkers of mercury exposure in fish. |
publishDate |
2023 |
dc.date.none.fl_str_mv |
2023-07-29T16:02:50Z 2023-07-29T16:02:50Z 2023-03-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1016/j.chemosphere.2023.137779 Chemosphere, v. 316. 1879-1298 0045-6535 http://hdl.handle.net/11449/249549 10.1016/j.chemosphere.2023.137779 2-s2.0-85146161032 |
url |
http://dx.doi.org/10.1016/j.chemosphere.2023.137779 http://hdl.handle.net/11449/249549 |
identifier_str_mv |
Chemosphere, v. 316. 1879-1298 0045-6535 10.1016/j.chemosphere.2023.137779 2-s2.0-85146161032 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Chemosphere |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
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1799965171772293120 |