Two successful embryo transfers of mini-donkey embryos in Brazilian Northeastern jennies using an alternative method: Case report
Autor(a) principal: | |
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Data de Publicação: | 2021 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1111/rda.14000 http://hdl.handle.net/11449/229313 |
Resumo: | Pregnancy rates after embryo transfer (ET) are disappointing in donkey species. This study aims to report two successful ET of mini-donkey embryos using Brazilian Northeastern jennies as recipients. Eighteen embryo flushes were performed 9 days post-ovulation in two non-pregnant mini-donkeys jennies (11 and 7 cycles per jenny). Eleven embryos (61%, 11/18) were collected and transferred to Brazilian Northeastern jennies 4–6 days post-ovulation by conventional (n = 6) or an alternative (n = 5) technique. The alternative method consisted of inserting a Polansky equine vaginal speculum smeared with lubricant in the vagina of the recipient jenny. The arms of the speculum were extended to allow the visualization of the cervix. Then, using an adapted crafted, elongated, toothed tissue grasping forceps, the external cervical os was held, and the cervix was gently pulled backward, aiming to straight the cervical canal. The ET gun was inserted through the vagina and cervix by visual inspection, and the embryo was released into the uterine lumen. All embryos collected were Grade 1 and classified as Expanded Blastocysts. No jennies become pregnant after conventional ET (0/6), whereas two recipient jennies (40%, 2/5) become pregnant and delivered offspring in the following year after ET using the alternative technique. In conclusion, Brazilian Northeastern jennies can be used as embryo recipients using the alternative method proposed in the present study. However, further investigations are needed to improve the knowledge and results of ET in donkey species. |
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Two successful embryo transfers of mini-donkey embryos in Brazilian Northeastern jennies using an alternative method: Case reportequinefertilityminiature horsereproductionPregnancy rates after embryo transfer (ET) are disappointing in donkey species. This study aims to report two successful ET of mini-donkey embryos using Brazilian Northeastern jennies as recipients. Eighteen embryo flushes were performed 9 days post-ovulation in two non-pregnant mini-donkeys jennies (11 and 7 cycles per jenny). Eleven embryos (61%, 11/18) were collected and transferred to Brazilian Northeastern jennies 4–6 days post-ovulation by conventional (n = 6) or an alternative (n = 5) technique. The alternative method consisted of inserting a Polansky equine vaginal speculum smeared with lubricant in the vagina of the recipient jenny. The arms of the speculum were extended to allow the visualization of the cervix. Then, using an adapted crafted, elongated, toothed tissue grasping forceps, the external cervical os was held, and the cervix was gently pulled backward, aiming to straight the cervical canal. The ET gun was inserted through the vagina and cervix by visual inspection, and the embryo was released into the uterine lumen. All embryos collected were Grade 1 and classified as Expanded Blastocysts. No jennies become pregnant after conventional ET (0/6), whereas two recipient jennies (40%, 2/5) become pregnant and delivered offspring in the following year after ET using the alternative technique. In conclusion, Brazilian Northeastern jennies can be used as embryo recipients using the alternative method proposed in the present study. However, further investigations are needed to improve the knowledge and results of ET in donkey species.Department of Veterinary Surgery and Animal Reproduction School of Veterinary Medicine and Animal Science São Paulo State University UNESPRoss University School of Veterinary MedicineDepartment of Veterinary Surgery and Animal Reproduction School of Veterinary Medicine and Animal Science São Paulo State University UNESPUniversidade Estadual Paulista (UNESP)Ross University School of Veterinary MedicineRodrigues, Lucas T. [UNESP]Segabinazzi, Lorenzo GTM [UNESP]Oliveira, Sidnei N [UNESP]Frasson, Mariana [UNESP]Papa, Frederico O [UNESP]2022-04-29T08:31:51Z2022-04-29T08:31:51Z2021-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.1111/rda.14000Reproduction in Domestic Animals.1439-05310936-6768http://hdl.handle.net/11449/22931310.1111/rda.140002-s2.0-85112456113Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengReproduction in Domestic Animalsinfo:eu-repo/semantics/openAccess2024-09-09T14:01:19Zoai:repositorio.unesp.br:11449/229313Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestrepositoriounesp@unesp.bropendoar:29462024-09-09T14:01:19Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Two successful embryo transfers of mini-donkey embryos in Brazilian Northeastern jennies using an alternative method: Case report |
title |
Two successful embryo transfers of mini-donkey embryos in Brazilian Northeastern jennies using an alternative method: Case report |
spellingShingle |
Two successful embryo transfers of mini-donkey embryos in Brazilian Northeastern jennies using an alternative method: Case report Rodrigues, Lucas T. [UNESP] equine fertility miniature horse reproduction |
title_short |
Two successful embryo transfers of mini-donkey embryos in Brazilian Northeastern jennies using an alternative method: Case report |
title_full |
Two successful embryo transfers of mini-donkey embryos in Brazilian Northeastern jennies using an alternative method: Case report |
title_fullStr |
Two successful embryo transfers of mini-donkey embryos in Brazilian Northeastern jennies using an alternative method: Case report |
title_full_unstemmed |
Two successful embryo transfers of mini-donkey embryos in Brazilian Northeastern jennies using an alternative method: Case report |
title_sort |
Two successful embryo transfers of mini-donkey embryos in Brazilian Northeastern jennies using an alternative method: Case report |
author |
Rodrigues, Lucas T. [UNESP] |
author_facet |
Rodrigues, Lucas T. [UNESP] Segabinazzi, Lorenzo GTM [UNESP] Oliveira, Sidnei N [UNESP] Frasson, Mariana [UNESP] Papa, Frederico O [UNESP] |
author_role |
author |
author2 |
Segabinazzi, Lorenzo GTM [UNESP] Oliveira, Sidnei N [UNESP] Frasson, Mariana [UNESP] Papa, Frederico O [UNESP] |
author2_role |
author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (UNESP) Ross University School of Veterinary Medicine |
dc.contributor.author.fl_str_mv |
Rodrigues, Lucas T. [UNESP] Segabinazzi, Lorenzo GTM [UNESP] Oliveira, Sidnei N [UNESP] Frasson, Mariana [UNESP] Papa, Frederico O [UNESP] |
dc.subject.por.fl_str_mv |
equine fertility miniature horse reproduction |
topic |
equine fertility miniature horse reproduction |
description |
Pregnancy rates after embryo transfer (ET) are disappointing in donkey species. This study aims to report two successful ET of mini-donkey embryos using Brazilian Northeastern jennies as recipients. Eighteen embryo flushes were performed 9 days post-ovulation in two non-pregnant mini-donkeys jennies (11 and 7 cycles per jenny). Eleven embryos (61%, 11/18) were collected and transferred to Brazilian Northeastern jennies 4–6 days post-ovulation by conventional (n = 6) or an alternative (n = 5) technique. The alternative method consisted of inserting a Polansky equine vaginal speculum smeared with lubricant in the vagina of the recipient jenny. The arms of the speculum were extended to allow the visualization of the cervix. Then, using an adapted crafted, elongated, toothed tissue grasping forceps, the external cervical os was held, and the cervix was gently pulled backward, aiming to straight the cervical canal. The ET gun was inserted through the vagina and cervix by visual inspection, and the embryo was released into the uterine lumen. All embryos collected were Grade 1 and classified as Expanded Blastocysts. No jennies become pregnant after conventional ET (0/6), whereas two recipient jennies (40%, 2/5) become pregnant and delivered offspring in the following year after ET using the alternative technique. In conclusion, Brazilian Northeastern jennies can be used as embryo recipients using the alternative method proposed in the present study. However, further investigations are needed to improve the knowledge and results of ET in donkey species. |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021-01-01 2022-04-29T08:31:51Z 2022-04-29T08:31:51Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1111/rda.14000 Reproduction in Domestic Animals. 1439-0531 0936-6768 http://hdl.handle.net/11449/229313 10.1111/rda.14000 2-s2.0-85112456113 |
url |
http://dx.doi.org/10.1111/rda.14000 http://hdl.handle.net/11449/229313 |
identifier_str_mv |
Reproduction in Domestic Animals. 1439-0531 0936-6768 10.1111/rda.14000 2-s2.0-85112456113 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Reproduction in Domestic Animals |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
repositoriounesp@unesp.br |
_version_ |
1810021324489228288 |