Coconut Water as an Extender Component for Cooled Equine Sperm
Autor(a) principal: | |
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Data de Publicação: | 2019 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1016/j.jevs.2019.03.213 http://hdl.handle.net/11449/185804 |
Resumo: | The aim of this study was to evaluate the effect of coconut water as a component of extender in different formulations for cooling equine sperm. One ejaculate of fourteen stallions was collected. Sperm was diluted to 50 x 10(6) sperm/mL using five different extenders: ACP-105: powdered coconut water extender (ACP-105, ACP Biotecnologia, Brazil); ACP-Milk: ACP-105 + 20 g/L of skimmed milk; ACP-EY 2.5%: ACP105 + 2.5% of egg yolk; ACP-EY 5%: ACP-105 + 5% of egg yolk; and BotuSemen (Botupharma, Botucatu, Brazil) and cooled in passive cooling device (BotuFlex, Botupharma, Botucatu, Brazil) at 5 and 15 degrees C for 24 hours. Sperm kinetics and plasma membrane integrity (PMI) were evaluated by computer-assisted sperm analysis and fluorescence staining, respectively, at TO (before cooling) and T24 (24 hours after cooling). Sperm kinetics did not differ at TO among groups (P > .05); however, at T24, these parameters were significantly lower in ACP-105 (5 degrees C, total motility [TM]: 9.2 +/- 3.6%; progressive motility [PM]: 2.7 +/- 1.6%; percentage of fast-moving spermatozoa [RAP]: 4.8 +/- 3.0%; 15 degrees C, TM: 10.6 +/- 3.0%; PM: 1.1 +/- 0.5%; RAP: 4.8 +/- 1.9%) and ACP-EY 5% (5 degrees C, TM: 28.0 +/- 6.3%; PM: 5.7 +/- 1.8%; RAP: 15.9 +/- 6.0%; 15 degrees C, TM: 30.0 +/- 6.0%; PM: 6.9 +/- 2.1%; RAP: 17.6 +/- 5.3%) compared with BotuSemen (5 degrees C, TM: 66.2 +/- 5.6%; PM: 21.1 +/- 2.8%; RAP: 53.9 +/- 6.1%; 15 degrees C, TM: 63.4 +/- 5.4%; PM: 17.2 +/- 2.8%; RAP: 51.4 +/- 6.3%) (P < .05). All groups exhibited similar PMI at tested moments and cooling temperatures (5 degrees C: 83%; 15 degrees C: 84%) (P> .05). Further studies are necessary to evaluate powdered coconut water in different compositions of sperm extender; however, coconut-based extender as used in this study was not an alternative to preserve sperm parameters of cooled equine sperm. (C) 2019 Elsevier Inc. All rights reserved. |
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Coconut Water as an Extender Component for Cooled Equine SpermSemenstallionequine reproductionfertilityThe aim of this study was to evaluate the effect of coconut water as a component of extender in different formulations for cooling equine sperm. One ejaculate of fourteen stallions was collected. Sperm was diluted to 50 x 10(6) sperm/mL using five different extenders: ACP-105: powdered coconut water extender (ACP-105, ACP Biotecnologia, Brazil); ACP-Milk: ACP-105 + 20 g/L of skimmed milk; ACP-EY 2.5%: ACP105 + 2.5% of egg yolk; ACP-EY 5%: ACP-105 + 5% of egg yolk; and BotuSemen (Botupharma, Botucatu, Brazil) and cooled in passive cooling device (BotuFlex, Botupharma, Botucatu, Brazil) at 5 and 15 degrees C for 24 hours. Sperm kinetics and plasma membrane integrity (PMI) were evaluated by computer-assisted sperm analysis and fluorescence staining, respectively, at TO (before cooling) and T24 (24 hours after cooling). Sperm kinetics did not differ at TO among groups (P > .05); however, at T24, these parameters were significantly lower in ACP-105 (5 degrees C, total motility [TM]: 9.2 +/- 3.6%; progressive motility [PM]: 2.7 +/- 1.6%; percentage of fast-moving spermatozoa [RAP]: 4.8 +/- 3.0%; 15 degrees C, TM: 10.6 +/- 3.0%; PM: 1.1 +/- 0.5%; RAP: 4.8 +/- 1.9%) and ACP-EY 5% (5 degrees C, TM: 28.0 +/- 6.3%; PM: 5.7 +/- 1.8%; RAP: 15.9 +/- 6.0%; 15 degrees C, TM: 30.0 +/- 6.0%; PM: 6.9 +/- 2.1%; RAP: 17.6 +/- 5.3%) compared with BotuSemen (5 degrees C, TM: 66.2 +/- 5.6%; PM: 21.1 +/- 2.8%; RAP: 53.9 +/- 6.1%; 15 degrees C, TM: 63.4 +/- 5.4%; PM: 17.2 +/- 2.8%; RAP: 51.4 +/- 6.3%) (P < .05). All groups exhibited similar PMI at tested moments and cooling temperatures (5 degrees C: 83%; 15 degrees C: 84%) (P> .05). Further studies are necessary to evaluate powdered coconut water in different compositions of sperm extender; however, coconut-based extender as used in this study was not an alternative to preserve sperm parameters of cooled equine sperm. (C) 2019 Elsevier Inc. All rights reserved.Univ Estadual Ceara, Dept Vet Med, Fortaleza, Ceara, BrazilSao Paulo State Univ, Dept Anim Reprod & Vet Radiol, Sch Vet Med & Anim Sci, Botucatu, SP, BrazilSao Paulo State Univ, Dept Anim Reprod & Vet Radiol, Sch Vet Med & Anim Sci, Botucatu, SP, BrazilElsevier B.V.Univ Estadual CearaUniversidade Estadual Paulista (Unesp)Brasileiro, Lucas S.Teixeira M Segabinazzi, Lorenzo Garrido [UNESP]Menezes, ErikaSalgueiro, Cristiane C.Novello, Guilherme [UNESP]Cunha Scheeren, Veronica Flores da [UNESP]Alvarenga, Marco Antonio [UNESP]Nunes, Jose Ferreira2019-10-04T12:38:43Z2019-10-04T12:38:43Z2019-07-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article69-73http://dx.doi.org/10.1016/j.jevs.2019.03.213Journal Of Equine Veterinary Science. New York: Elsevier Science Inc, v. 78, p. 69-73, 2019.0737-0806http://hdl.handle.net/11449/18580410.1016/j.jevs.2019.03.213WOS:0004716467000110473846154288947Web of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengJournal Of Equine Veterinary Scienceinfo:eu-repo/semantics/openAccess2021-10-22T18:13:07Zoai:repositorio.unesp.br:11449/185804Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462021-10-22T18:13:07Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Coconut Water as an Extender Component for Cooled Equine Sperm |
title |
Coconut Water as an Extender Component for Cooled Equine Sperm |
spellingShingle |
Coconut Water as an Extender Component for Cooled Equine Sperm Brasileiro, Lucas S. Semen stallion equine reproduction fertility |
title_short |
Coconut Water as an Extender Component for Cooled Equine Sperm |
title_full |
Coconut Water as an Extender Component for Cooled Equine Sperm |
title_fullStr |
Coconut Water as an Extender Component for Cooled Equine Sperm |
title_full_unstemmed |
Coconut Water as an Extender Component for Cooled Equine Sperm |
title_sort |
Coconut Water as an Extender Component for Cooled Equine Sperm |
author |
Brasileiro, Lucas S. |
author_facet |
Brasileiro, Lucas S. Teixeira M Segabinazzi, Lorenzo Garrido [UNESP] Menezes, Erika Salgueiro, Cristiane C. Novello, Guilherme [UNESP] Cunha Scheeren, Veronica Flores da [UNESP] Alvarenga, Marco Antonio [UNESP] Nunes, Jose Ferreira |
author_role |
author |
author2 |
Teixeira M Segabinazzi, Lorenzo Garrido [UNESP] Menezes, Erika Salgueiro, Cristiane C. Novello, Guilherme [UNESP] Cunha Scheeren, Veronica Flores da [UNESP] Alvarenga, Marco Antonio [UNESP] Nunes, Jose Ferreira |
author2_role |
author author author author author author author |
dc.contributor.none.fl_str_mv |
Univ Estadual Ceara Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Brasileiro, Lucas S. Teixeira M Segabinazzi, Lorenzo Garrido [UNESP] Menezes, Erika Salgueiro, Cristiane C. Novello, Guilherme [UNESP] Cunha Scheeren, Veronica Flores da [UNESP] Alvarenga, Marco Antonio [UNESP] Nunes, Jose Ferreira |
dc.subject.por.fl_str_mv |
Semen stallion equine reproduction fertility |
topic |
Semen stallion equine reproduction fertility |
description |
The aim of this study was to evaluate the effect of coconut water as a component of extender in different formulations for cooling equine sperm. One ejaculate of fourteen stallions was collected. Sperm was diluted to 50 x 10(6) sperm/mL using five different extenders: ACP-105: powdered coconut water extender (ACP-105, ACP Biotecnologia, Brazil); ACP-Milk: ACP-105 + 20 g/L of skimmed milk; ACP-EY 2.5%: ACP105 + 2.5% of egg yolk; ACP-EY 5%: ACP-105 + 5% of egg yolk; and BotuSemen (Botupharma, Botucatu, Brazil) and cooled in passive cooling device (BotuFlex, Botupharma, Botucatu, Brazil) at 5 and 15 degrees C for 24 hours. Sperm kinetics and plasma membrane integrity (PMI) were evaluated by computer-assisted sperm analysis and fluorescence staining, respectively, at TO (before cooling) and T24 (24 hours after cooling). Sperm kinetics did not differ at TO among groups (P > .05); however, at T24, these parameters were significantly lower in ACP-105 (5 degrees C, total motility [TM]: 9.2 +/- 3.6%; progressive motility [PM]: 2.7 +/- 1.6%; percentage of fast-moving spermatozoa [RAP]: 4.8 +/- 3.0%; 15 degrees C, TM: 10.6 +/- 3.0%; PM: 1.1 +/- 0.5%; RAP: 4.8 +/- 1.9%) and ACP-EY 5% (5 degrees C, TM: 28.0 +/- 6.3%; PM: 5.7 +/- 1.8%; RAP: 15.9 +/- 6.0%; 15 degrees C, TM: 30.0 +/- 6.0%; PM: 6.9 +/- 2.1%; RAP: 17.6 +/- 5.3%) compared with BotuSemen (5 degrees C, TM: 66.2 +/- 5.6%; PM: 21.1 +/- 2.8%; RAP: 53.9 +/- 6.1%; 15 degrees C, TM: 63.4 +/- 5.4%; PM: 17.2 +/- 2.8%; RAP: 51.4 +/- 6.3%) (P < .05). All groups exhibited similar PMI at tested moments and cooling temperatures (5 degrees C: 83%; 15 degrees C: 84%) (P> .05). Further studies are necessary to evaluate powdered coconut water in different compositions of sperm extender; however, coconut-based extender as used in this study was not an alternative to preserve sperm parameters of cooled equine sperm. (C) 2019 Elsevier Inc. All rights reserved. |
publishDate |
2019 |
dc.date.none.fl_str_mv |
2019-10-04T12:38:43Z 2019-10-04T12:38:43Z 2019-07-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1016/j.jevs.2019.03.213 Journal Of Equine Veterinary Science. New York: Elsevier Science Inc, v. 78, p. 69-73, 2019. 0737-0806 http://hdl.handle.net/11449/185804 10.1016/j.jevs.2019.03.213 WOS:000471646700011 0473846154288947 |
url |
http://dx.doi.org/10.1016/j.jevs.2019.03.213 http://hdl.handle.net/11449/185804 |
identifier_str_mv |
Journal Of Equine Veterinary Science. New York: Elsevier Science Inc, v. 78, p. 69-73, 2019. 0737-0806 10.1016/j.jevs.2019.03.213 WOS:000471646700011 0473846154288947 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Journal Of Equine Veterinary Science |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
69-73 |
dc.publisher.none.fl_str_mv |
Elsevier B.V. |
publisher.none.fl_str_mv |
Elsevier B.V. |
dc.source.none.fl_str_mv |
Web of Science reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1799964723589939200 |