Pediocin PA-1 production by Pediococcus pentosaceus ET34 using non-detoxified hemicellulose hydrolysate obtained from hydrothermal pretreatment of sugarcane bagasse

• Autor(a) principal: Kuniyoshi, Tais Mayumi
• Data de Publicação: 2021
• Outros Autores: Nobrega Mendonca, Carlos Miguel, Vieira, Viviane Borges, Robl, Diogo, Gombossy de Melo Franco, Bernadette Dora, Todorov, Svetoslav Dimitrov, Tome, Elisabetta, O'Connor, Paula Mary, Converti, Attilio, Araujo, Welington Luiz, Silva Palmeiras Vasconcellos, Livia Paula, Varani, Alessandro de Mello [UNESP], Cotter, Paul David, Rabelo, Sarita Candida [UNESP], Souza Oliveira, Ricardo Pinheiro de
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Repositório Institucional da UNESP
• Texto Completo: http://dx.doi.org/10.1016/j.biortech.2021.125565; http://hdl.handle.net/11449/218647
• topic: Sugarcane bagasse; Bacteriocin; Hemicellulose hydrolysate; < em > Pediococcus pentosaceus <; em >

Listeria monocytogenes is one of the foodborne pathogens of most concern for food safety. To limit its presence in foods, bacteriocins have been proposed as natural bio-preservatives. Herein, a bacteriocin was produced on hemicellulose hydrolysate of sugarcane bagasse by Pediococcus pentosaceous ET34, whose genome sequencing revealed an operon with 100% similarity to that of pediocin PA-1. ET34 grown on hydrolysate-containing medium led to an increase in the expression of PA-1 genes and a non-optimized purification step sequence resulted in a yield of 0.8 mg & sdot;L-1 of pure pediocin (purity > 95%). Culture conditions were optimized according to a central composite design using temperature and hydrolysate % as independent variables and validated in 3-L Erlenmeyers. Finally, a process for scaled-up implementation by sugar-ethanol industry was proposed, considering green chemistry and biorefinery concepts. This work stands up as an approach addressing a future proper sugarcane bagasse valorisation for pediocin production. <comment>Superscript/Subscript Available</comment