Gene expression, immunohistochemical and microarchitectural evaluation of bone formation around two implant surfaces placed in bone defects filled or not with bone substitute material
Autor(a) principal: | |
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Data de Publicação: | 2020 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1186/s40729-020-00279-7 http://hdl.handle.net/11449/209738 |
Resumo: | Objective The aim of this study is to evaluate through gene expression, immunohistochemical and microtomographic (micro-CT) analysis the response of peri-implant bone tissue around titanium implants with different surface treatments, placed in bone defects filled or not with bone substitute materials. In addition, to investigate the hypothesis that porous-hydrophilic surface induces a faster bone formation. Materials and methods Twenty-six animals were divided into two groups according to implant surface treatment. In each tibia, a bone defect was created followed by the placement of one implant. On the left tibia, the defect was filled with blood clot (BC), and on the right tibia, the defect was filled with biphasic hydroxyapatite/beta-tricalcium-phosphate (HA/TCP) generating four subgroups: BC-N: bone defect filled with blood clot and porous surface titanium implant installed; BC-A: bone defect filled with blood clot and porous-hydrophilic surface titanium implant installed; HA/TCP-N: bone defect filled with bone substitute material and porous surface titanium implant installed; and HA/TCP-A: bone defect filled with bone substitute material and porous-hydrophilic surface titanium implant installed. The animals were submitted to euthanasia at 15, 30, and 60 days after implant installation. The expression of two genes was evaluated: RUNX2 and BSP. Immunohistochemical analyses were performed for detection of RUNX2, OPN, OCN, OPG, and RANKL antibodies and bone matrix proteins. Finally, four parameters were chosen for micro-CT analysis: trabecular number, separation and thickness, and connectivity density. Results Descriptive analysis showed similar findings among the experimental groups. Moreover, porous-hydrophilic surfaces presented a higher expression of RUNX2, which is probably an indicative of better osteogenesis; although the data from this study may be considered an insufficient support for a concrete statement. Conclusion Porous hydrophilic surface can improve and accelerate protein expression and bone formation. |
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Gene expression, immunohistochemical and microarchitectural evaluation of bone formation around two implant surfaces placed in bone defects filled or not with bone substitute materialOsseointegrated dental implantationBone graftingCell viabilityObjective The aim of this study is to evaluate through gene expression, immunohistochemical and microtomographic (micro-CT) analysis the response of peri-implant bone tissue around titanium implants with different surface treatments, placed in bone defects filled or not with bone substitute materials. In addition, to investigate the hypothesis that porous-hydrophilic surface induces a faster bone formation. Materials and methods Twenty-six animals were divided into two groups according to implant surface treatment. In each tibia, a bone defect was created followed by the placement of one implant. On the left tibia, the defect was filled with blood clot (BC), and on the right tibia, the defect was filled with biphasic hydroxyapatite/beta-tricalcium-phosphate (HA/TCP) generating four subgroups: BC-N: bone defect filled with blood clot and porous surface titanium implant installed; BC-A: bone defect filled with blood clot and porous-hydrophilic surface titanium implant installed; HA/TCP-N: bone defect filled with bone substitute material and porous surface titanium implant installed; and HA/TCP-A: bone defect filled with bone substitute material and porous-hydrophilic surface titanium implant installed. The animals were submitted to euthanasia at 15, 30, and 60 days after implant installation. The expression of two genes was evaluated: RUNX2 and BSP. Immunohistochemical analyses were performed for detection of RUNX2, OPN, OCN, OPG, and RANKL antibodies and bone matrix proteins. Finally, four parameters were chosen for micro-CT analysis: trabecular number, separation and thickness, and connectivity density. Results Descriptive analysis showed similar findings among the experimental groups. Moreover, porous-hydrophilic surfaces presented a higher expression of RUNX2, which is probably an indicative of better osteogenesis; although the data from this study may be considered an insufficient support for a concrete statement. Conclusion Porous hydrophilic surface can improve and accelerate protein expression and bone formation.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Sao Paulo State Univ Unesp, Sch Dent, Dept Diag & Surg, 1680th Humaita St, BR-14801903 Araraquara, SP, BrazilSao Paulo State Univ Unesp, Sch Dent, Dept Oral & Maxillofacial Surg, Aracatuba, BrazilSao Paulo State Univ Unesp, Sch Dent, Dept Diag & Surg, 1680th Humaita St, BR-14801903 Araraquara, SP, BrazilSao Paulo State Univ Unesp, Sch Dent, Dept Oral & Maxillofacial Surg, Aracatuba, BrazilFAPESP: 2016/07837-1FAPESP: 2017/04017-6SpringerUniversidade Estadual Paulista (Unesp)Trento, Guilherme dos Santos [UNESP]Hassumi, Jaqueline Suemi [UNESP]Frigerio, Paula Buzo [UNESP]Farnezi Bassi, Ana Paula [UNESP]Okamoto, Roberta [UNESP]Cabrini Gabrielli, Marisa Aparecida [UNESP]Pereira-Filho, Valfrido Antonio [UNESP]2021-06-25T12:27:34Z2021-06-25T12:27:34Z2020-12-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article14http://dx.doi.org/10.1186/s40729-020-00279-7International Journal Of Implant Dentistry. Tokyo: Springer Japan Kk, v. 6, n. 1, 14 p., 2020.http://hdl.handle.net/11449/20973810.1186/s40729-020-00279-7WOS:000595287800001Web of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengInternational Journal Of Implant Dentistryinfo:eu-repo/semantics/openAccess2021-10-23T19:49:59Zoai:repositorio.unesp.br:11449/209738Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T23:09:32.667589Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Gene expression, immunohistochemical and microarchitectural evaluation of bone formation around two implant surfaces placed in bone defects filled or not with bone substitute material |
title |
Gene expression, immunohistochemical and microarchitectural evaluation of bone formation around two implant surfaces placed in bone defects filled or not with bone substitute material |
spellingShingle |
Gene expression, immunohistochemical and microarchitectural evaluation of bone formation around two implant surfaces placed in bone defects filled or not with bone substitute material Trento, Guilherme dos Santos [UNESP] Osseointegrated dental implantation Bone grafting Cell viability |
title_short |
Gene expression, immunohistochemical and microarchitectural evaluation of bone formation around two implant surfaces placed in bone defects filled or not with bone substitute material |
title_full |
Gene expression, immunohistochemical and microarchitectural evaluation of bone formation around two implant surfaces placed in bone defects filled or not with bone substitute material |
title_fullStr |
Gene expression, immunohistochemical and microarchitectural evaluation of bone formation around two implant surfaces placed in bone defects filled or not with bone substitute material |
title_full_unstemmed |
Gene expression, immunohistochemical and microarchitectural evaluation of bone formation around two implant surfaces placed in bone defects filled or not with bone substitute material |
title_sort |
Gene expression, immunohistochemical and microarchitectural evaluation of bone formation around two implant surfaces placed in bone defects filled or not with bone substitute material |
author |
Trento, Guilherme dos Santos [UNESP] |
author_facet |
Trento, Guilherme dos Santos [UNESP] Hassumi, Jaqueline Suemi [UNESP] Frigerio, Paula Buzo [UNESP] Farnezi Bassi, Ana Paula [UNESP] Okamoto, Roberta [UNESP] Cabrini Gabrielli, Marisa Aparecida [UNESP] Pereira-Filho, Valfrido Antonio [UNESP] |
author_role |
author |
author2 |
Hassumi, Jaqueline Suemi [UNESP] Frigerio, Paula Buzo [UNESP] Farnezi Bassi, Ana Paula [UNESP] Okamoto, Roberta [UNESP] Cabrini Gabrielli, Marisa Aparecida [UNESP] Pereira-Filho, Valfrido Antonio [UNESP] |
author2_role |
author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Trento, Guilherme dos Santos [UNESP] Hassumi, Jaqueline Suemi [UNESP] Frigerio, Paula Buzo [UNESP] Farnezi Bassi, Ana Paula [UNESP] Okamoto, Roberta [UNESP] Cabrini Gabrielli, Marisa Aparecida [UNESP] Pereira-Filho, Valfrido Antonio [UNESP] |
dc.subject.por.fl_str_mv |
Osseointegrated dental implantation Bone grafting Cell viability |
topic |
Osseointegrated dental implantation Bone grafting Cell viability |
description |
Objective The aim of this study is to evaluate through gene expression, immunohistochemical and microtomographic (micro-CT) analysis the response of peri-implant bone tissue around titanium implants with different surface treatments, placed in bone defects filled or not with bone substitute materials. In addition, to investigate the hypothesis that porous-hydrophilic surface induces a faster bone formation. Materials and methods Twenty-six animals were divided into two groups according to implant surface treatment. In each tibia, a bone defect was created followed by the placement of one implant. On the left tibia, the defect was filled with blood clot (BC), and on the right tibia, the defect was filled with biphasic hydroxyapatite/beta-tricalcium-phosphate (HA/TCP) generating four subgroups: BC-N: bone defect filled with blood clot and porous surface titanium implant installed; BC-A: bone defect filled with blood clot and porous-hydrophilic surface titanium implant installed; HA/TCP-N: bone defect filled with bone substitute material and porous surface titanium implant installed; and HA/TCP-A: bone defect filled with bone substitute material and porous-hydrophilic surface titanium implant installed. The animals were submitted to euthanasia at 15, 30, and 60 days after implant installation. The expression of two genes was evaluated: RUNX2 and BSP. Immunohistochemical analyses were performed for detection of RUNX2, OPN, OCN, OPG, and RANKL antibodies and bone matrix proteins. Finally, four parameters were chosen for micro-CT analysis: trabecular number, separation and thickness, and connectivity density. Results Descriptive analysis showed similar findings among the experimental groups. Moreover, porous-hydrophilic surfaces presented a higher expression of RUNX2, which is probably an indicative of better osteogenesis; although the data from this study may be considered an insufficient support for a concrete statement. Conclusion Porous hydrophilic surface can improve and accelerate protein expression and bone formation. |
publishDate |
2020 |
dc.date.none.fl_str_mv |
2020-12-01 2021-06-25T12:27:34Z 2021-06-25T12:27:34Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1186/s40729-020-00279-7 International Journal Of Implant Dentistry. Tokyo: Springer Japan Kk, v. 6, n. 1, 14 p., 2020. http://hdl.handle.net/11449/209738 10.1186/s40729-020-00279-7 WOS:000595287800001 |
url |
http://dx.doi.org/10.1186/s40729-020-00279-7 http://hdl.handle.net/11449/209738 |
identifier_str_mv |
International Journal Of Implant Dentistry. Tokyo: Springer Japan Kk, v. 6, n. 1, 14 p., 2020. 10.1186/s40729-020-00279-7 WOS:000595287800001 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
International Journal Of Implant Dentistry |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
14 |
dc.publisher.none.fl_str_mv |
Springer |
publisher.none.fl_str_mv |
Springer |
dc.source.none.fl_str_mv |
Web of Science reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808129495715020800 |