Bacterial cellulose-hydroxyapatite nanocomposites for bone regeneration
Autor(a) principal: | |
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Data de Publicação: | 2011 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1155/2011/175362 http://hdl.handle.net/11449/72814 |
Resumo: | The aim of this study was to develop and to evaluate the biological properties of bacterial cellulose-hydroxyapatite (BC-HA) nanocomposite membranes for bone regeneration. Nanocomposites were prepared from bacterial cellulose membranes sequentially incubated in solutions of CaCl2 followed by Na2HPO4. BC-HA membranes were evaluated in noncritical bone defects in rat tibiae at 1, 4, and 16 weeks. Thermogravimetric analyses showed that the amount of the mineral phase was 40-50 of the total weight. Spectroscopy, electronic microscopy/energy dispersive X-ray analyses, and X-ray diffraction showed formation of HA crystals on BC nanofibres. Low crystallinity HA crystals presented Ca/P a molar ratio of 1.5 (calcium-deficient HA), similar to physiological bone. Fourier transformed infrared spectroscopy analysis showed bands assigned to phosphate and carbonate ions. In vivo tests showed no inflammatory reaction after 1 week. After 4 weeks, defects were observed to be completely filled in by new bone tissue. The BC-HA membranes were effective for bone regeneration. © 2011 S. Saska et al. |
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Bacterial cellulose-hydroxyapatite nanocomposites for bone regenerationThe aim of this study was to develop and to evaluate the biological properties of bacterial cellulose-hydroxyapatite (BC-HA) nanocomposite membranes for bone regeneration. Nanocomposites were prepared from bacterial cellulose membranes sequentially incubated in solutions of CaCl2 followed by Na2HPO4. BC-HA membranes were evaluated in noncritical bone defects in rat tibiae at 1, 4, and 16 weeks. Thermogravimetric analyses showed that the amount of the mineral phase was 40-50 of the total weight. Spectroscopy, electronic microscopy/energy dispersive X-ray analyses, and X-ray diffraction showed formation of HA crystals on BC nanofibres. Low crystallinity HA crystals presented Ca/P a molar ratio of 1.5 (calcium-deficient HA), similar to physiological bone. Fourier transformed infrared spectroscopy analysis showed bands assigned to phosphate and carbonate ions. In vivo tests showed no inflammatory reaction after 1 week. After 4 weeks, defects were observed to be completely filled in by new bone tissue. The BC-HA membranes were effective for bone regeneration. © 2011 S. Saska et al.Institute of Chemistry University Estadual Paulista-UNESP, CP 355, 14-801-970 Araraquara, SPDepartment of Inorganic Chemistry Institute of ChemistryUNESP, Rua Francisco Degni s/n, 14-800-900 Araraquara, SPDepartment of Morphology Dental School University Estadual Paulista-UNESP, Rua Humaitá 1680, 14-801-903 Araraquara, SPInstitute of Chemistry University Estadual Paulista-UNESP, CP 355, 14-801-970 Araraquara, SPDepartment of Inorganic Chemistry Institute of ChemistryUNESP, Rua Francisco Degni s/n, 14-800-900 Araraquara, SPDepartment of Morphology Dental School University Estadual Paulista-UNESP, Rua Humaitá 1680, 14-801-903 Araraquara, SPUniversidade Estadual Paulista (Unesp)Saska, S. [UNESP]Barud, H. S. [UNESP]Gaspar, A. M M [UNESP]Marchetto, Reinaldo [UNESP]Ribeiro, Sidney José Lima [UNESP]Messaddeq, Younes [UNESP]2014-05-27T11:26:11Z2014-05-27T11:26:11Z2011-11-24info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://dx.doi.org/10.1155/2011/175362International Journal of Biomaterials.1687-87871687-8795http://hdl.handle.net/11449/7281410.1155/2011/1753622-s2.0-815552209062-s2.0-81555220906.pdf5711182251641103644604746303465429985038419178150000-0003-3286-9440Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengInternational Journal of Biomaterials0,5110,511info:eu-repo/semantics/openAccess2024-09-27T15:14:55Zoai:repositorio.unesp.br:11449/72814Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestrepositoriounesp@unesp.bropendoar:29462024-09-27T15:14:55Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Bacterial cellulose-hydroxyapatite nanocomposites for bone regeneration |
title |
Bacterial cellulose-hydroxyapatite nanocomposites for bone regeneration |
spellingShingle |
Bacterial cellulose-hydroxyapatite nanocomposites for bone regeneration Saska, S. [UNESP] |
title_short |
Bacterial cellulose-hydroxyapatite nanocomposites for bone regeneration |
title_full |
Bacterial cellulose-hydroxyapatite nanocomposites for bone regeneration |
title_fullStr |
Bacterial cellulose-hydroxyapatite nanocomposites for bone regeneration |
title_full_unstemmed |
Bacterial cellulose-hydroxyapatite nanocomposites for bone regeneration |
title_sort |
Bacterial cellulose-hydroxyapatite nanocomposites for bone regeneration |
author |
Saska, S. [UNESP] |
author_facet |
Saska, S. [UNESP] Barud, H. S. [UNESP] Gaspar, A. M M [UNESP] Marchetto, Reinaldo [UNESP] Ribeiro, Sidney José Lima [UNESP] Messaddeq, Younes [UNESP] |
author_role |
author |
author2 |
Barud, H. S. [UNESP] Gaspar, A. M M [UNESP] Marchetto, Reinaldo [UNESP] Ribeiro, Sidney José Lima [UNESP] Messaddeq, Younes [UNESP] |
author2_role |
author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Saska, S. [UNESP] Barud, H. S. [UNESP] Gaspar, A. M M [UNESP] Marchetto, Reinaldo [UNESP] Ribeiro, Sidney José Lima [UNESP] Messaddeq, Younes [UNESP] |
description |
The aim of this study was to develop and to evaluate the biological properties of bacterial cellulose-hydroxyapatite (BC-HA) nanocomposite membranes for bone regeneration. Nanocomposites were prepared from bacterial cellulose membranes sequentially incubated in solutions of CaCl2 followed by Na2HPO4. BC-HA membranes were evaluated in noncritical bone defects in rat tibiae at 1, 4, and 16 weeks. Thermogravimetric analyses showed that the amount of the mineral phase was 40-50 of the total weight. Spectroscopy, electronic microscopy/energy dispersive X-ray analyses, and X-ray diffraction showed formation of HA crystals on BC nanofibres. Low crystallinity HA crystals presented Ca/P a molar ratio of 1.5 (calcium-deficient HA), similar to physiological bone. Fourier transformed infrared spectroscopy analysis showed bands assigned to phosphate and carbonate ions. In vivo tests showed no inflammatory reaction after 1 week. After 4 weeks, defects were observed to be completely filled in by new bone tissue. The BC-HA membranes were effective for bone regeneration. © 2011 S. Saska et al. |
publishDate |
2011 |
dc.date.none.fl_str_mv |
2011-11-24 2014-05-27T11:26:11Z 2014-05-27T11:26:11Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1155/2011/175362 International Journal of Biomaterials. 1687-8787 1687-8795 http://hdl.handle.net/11449/72814 10.1155/2011/175362 2-s2.0-81555220906 2-s2.0-81555220906.pdf 5711182251641103 6446047463034654 2998503841917815 0000-0003-3286-9440 |
url |
http://dx.doi.org/10.1155/2011/175362 http://hdl.handle.net/11449/72814 |
identifier_str_mv |
International Journal of Biomaterials. 1687-8787 1687-8795 10.1155/2011/175362 2-s2.0-81555220906 2-s2.0-81555220906.pdf 5711182251641103 6446047463034654 2998503841917815 0000-0003-3286-9440 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
International Journal of Biomaterials 0,511 0,511 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
repositoriounesp@unesp.br |
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1813546373888344064 |