Water deprivation induces biochemical changes without reduction in the insecticidal activity of maize and soybean transgenic plants
Autor(a) principal: | |
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Data de Publicação: | 2021 |
Outros Autores: | , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1093/jee/toab109 http://hdl.handle.net/11449/233429 |
Resumo: | Like conventional crops, transgenic plants expressing insecticidal toxins from Bacillus thuringiensis (Bt) are subjected to water deprivation. However, the effects of water deprivation over the insecticidal activity of Bt plants are not well understood. We submitted Bt maize and Bt soybean to water deprivation and evaluated biochemical stress markers and the insecticidal activity of plants against target insects. Bt maize (DAS-Ø15Ø7-1 × MON-89Ø34-3 × MON-ØØ6Ø3-6 × SYN-IR162-4) containing the PowerCore Ultra traits, Bt soybean (DAS-444Ø6-6 × DAS-81419-2) with the Conkesta E3 traits, and commercial non-Bt cultivars were cultivated and exposed to water deprivation in the greenhouse. Leaves were harvested for quantification of hydrogen peroxide, malondialdeyde (MDA), and total phenolics and insecticidal activity. Maize or soybean leaf disks were used to evaluate the insecticidal activity against, respectively, Spodoptera frugiperda (J.E Smith) and Chrysodeixis includens (Walker) neonates. Except for Bt soybean, water deprivation increased hydrogen peroxide and MDA contents in Bt and non-Bt plants. Both biochemical markers of water deficit were observed in lower concentrations in Bt plants than in non-Bt commercial cultivars. Water deprivation did not result in changes of phenolic contents in Bt and non-Bt maize. For Bt or non-Bt soybean, phenolic contents were similar despite plants being exposed or not to water deprivation. Water deprivation did not alter substantially insect survival in non-Bt maize or non-Bt soybean. Despite water deprivation-induced biochemical changes in plants, both Bt plants maintained their insecticidal activity (100% mortality) against the target species. |
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Water deprivation induces biochemical changes without reduction in the insecticidal activity of maize and soybean transgenic plantsabiotic stressBt cropshydrogen peroxideinsect managementplant stressLike conventional crops, transgenic plants expressing insecticidal toxins from Bacillus thuringiensis (Bt) are subjected to water deprivation. However, the effects of water deprivation over the insecticidal activity of Bt plants are not well understood. We submitted Bt maize and Bt soybean to water deprivation and evaluated biochemical stress markers and the insecticidal activity of plants against target insects. Bt maize (DAS-Ø15Ø7-1 × MON-89Ø34-3 × MON-ØØ6Ø3-6 × SYN-IR162-4) containing the PowerCore Ultra traits, Bt soybean (DAS-444Ø6-6 × DAS-81419-2) with the Conkesta E3 traits, and commercial non-Bt cultivars were cultivated and exposed to water deprivation in the greenhouse. Leaves were harvested for quantification of hydrogen peroxide, malondialdeyde (MDA), and total phenolics and insecticidal activity. Maize or soybean leaf disks were used to evaluate the insecticidal activity against, respectively, Spodoptera frugiperda (J.E Smith) and Chrysodeixis includens (Walker) neonates. Except for Bt soybean, water deprivation increased hydrogen peroxide and MDA contents in Bt and non-Bt plants. Both biochemical markers of water deficit were observed in lower concentrations in Bt plants than in non-Bt commercial cultivars. Water deprivation did not result in changes of phenolic contents in Bt and non-Bt maize. For Bt or non-Bt soybean, phenolic contents were similar despite plants being exposed or not to water deprivation. Water deprivation did not alter substantially insect survival in non-Bt maize or non-Bt soybean. Despite water deprivation-induced biochemical changes in plants, both Bt plants maintained their insecticidal activity (100% mortality) against the target species.São Paulo State University (UNESP) School of Agricultural and Veterinarian SciencesCorteva Agriscience Alameda Itapecuru, 506, Alphaville - SPCorteva Agriscience, 7000NW 62nd AveSão Paulo State University (UNESP) School of Agricultural and Veterinarian SciencesUniversidade Estadual Paulista (UNESP)Alameda ItapecuruCorteva AgriscienceSilva, Juliana Barroso [UNESP]Mori, Raphael [UNESP]Marques, Luiz HenriqueSantos, Antonio CesarNowatzki, TimothyDahmer, Mark LindenBing, JamesGratão, Priscila Lupino [UNESP]Rossi, Guilherme Duarte [UNESP]2022-05-01T08:44:38Z2022-05-01T08:44:38Z2021-08-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article1817-1822http://dx.doi.org/10.1093/jee/toab109Journal of Economic Entomology, v. 114, n. 4, p. 1817-1822, 2021.1938-291X0022-0493http://hdl.handle.net/11449/23342910.1093/jee/toab1092-s2.0-85113288116Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengJournal of Economic Entomologyinfo:eu-repo/semantics/openAccess2024-06-06T13:04:11Zoai:repositorio.unesp.br:11449/233429Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T15:25:26.596701Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Water deprivation induces biochemical changes without reduction in the insecticidal activity of maize and soybean transgenic plants |
title |
Water deprivation induces biochemical changes without reduction in the insecticidal activity of maize and soybean transgenic plants |
spellingShingle |
Water deprivation induces biochemical changes without reduction in the insecticidal activity of maize and soybean transgenic plants Silva, Juliana Barroso [UNESP] abiotic stress Bt crops hydrogen peroxide insect management plant stress |
title_short |
Water deprivation induces biochemical changes without reduction in the insecticidal activity of maize and soybean transgenic plants |
title_full |
Water deprivation induces biochemical changes without reduction in the insecticidal activity of maize and soybean transgenic plants |
title_fullStr |
Water deprivation induces biochemical changes without reduction in the insecticidal activity of maize and soybean transgenic plants |
title_full_unstemmed |
Water deprivation induces biochemical changes without reduction in the insecticidal activity of maize and soybean transgenic plants |
title_sort |
Water deprivation induces biochemical changes without reduction in the insecticidal activity of maize and soybean transgenic plants |
author |
Silva, Juliana Barroso [UNESP] |
author_facet |
Silva, Juliana Barroso [UNESP] Mori, Raphael [UNESP] Marques, Luiz Henrique Santos, Antonio Cesar Nowatzki, Timothy Dahmer, Mark Linden Bing, James Gratão, Priscila Lupino [UNESP] Rossi, Guilherme Duarte [UNESP] |
author_role |
author |
author2 |
Mori, Raphael [UNESP] Marques, Luiz Henrique Santos, Antonio Cesar Nowatzki, Timothy Dahmer, Mark Linden Bing, James Gratão, Priscila Lupino [UNESP] Rossi, Guilherme Duarte [UNESP] |
author2_role |
author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (UNESP) Alameda Itapecuru Corteva Agriscience |
dc.contributor.author.fl_str_mv |
Silva, Juliana Barroso [UNESP] Mori, Raphael [UNESP] Marques, Luiz Henrique Santos, Antonio Cesar Nowatzki, Timothy Dahmer, Mark Linden Bing, James Gratão, Priscila Lupino [UNESP] Rossi, Guilherme Duarte [UNESP] |
dc.subject.por.fl_str_mv |
abiotic stress Bt crops hydrogen peroxide insect management plant stress |
topic |
abiotic stress Bt crops hydrogen peroxide insect management plant stress |
description |
Like conventional crops, transgenic plants expressing insecticidal toxins from Bacillus thuringiensis (Bt) are subjected to water deprivation. However, the effects of water deprivation over the insecticidal activity of Bt plants are not well understood. We submitted Bt maize and Bt soybean to water deprivation and evaluated biochemical stress markers and the insecticidal activity of plants against target insects. Bt maize (DAS-Ø15Ø7-1 × MON-89Ø34-3 × MON-ØØ6Ø3-6 × SYN-IR162-4) containing the PowerCore Ultra traits, Bt soybean (DAS-444Ø6-6 × DAS-81419-2) with the Conkesta E3 traits, and commercial non-Bt cultivars were cultivated and exposed to water deprivation in the greenhouse. Leaves were harvested for quantification of hydrogen peroxide, malondialdeyde (MDA), and total phenolics and insecticidal activity. Maize or soybean leaf disks were used to evaluate the insecticidal activity against, respectively, Spodoptera frugiperda (J.E Smith) and Chrysodeixis includens (Walker) neonates. Except for Bt soybean, water deprivation increased hydrogen peroxide and MDA contents in Bt and non-Bt plants. Both biochemical markers of water deficit were observed in lower concentrations in Bt plants than in non-Bt commercial cultivars. Water deprivation did not result in changes of phenolic contents in Bt and non-Bt maize. For Bt or non-Bt soybean, phenolic contents were similar despite plants being exposed or not to water deprivation. Water deprivation did not alter substantially insect survival in non-Bt maize or non-Bt soybean. Despite water deprivation-induced biochemical changes in plants, both Bt plants maintained their insecticidal activity (100% mortality) against the target species. |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021-08-01 2022-05-01T08:44:38Z 2022-05-01T08:44:38Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1093/jee/toab109 Journal of Economic Entomology, v. 114, n. 4, p. 1817-1822, 2021. 1938-291X 0022-0493 http://hdl.handle.net/11449/233429 10.1093/jee/toab109 2-s2.0-85113288116 |
url |
http://dx.doi.org/10.1093/jee/toab109 http://hdl.handle.net/11449/233429 |
identifier_str_mv |
Journal of Economic Entomology, v. 114, n. 4, p. 1817-1822, 2021. 1938-291X 0022-0493 10.1093/jee/toab109 2-s2.0-85113288116 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Journal of Economic Entomology |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
1817-1822 |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808128511810994176 |