Lower Susceptibility of Laser-irradiated Ti-15Mo Surface to Methicillin-resistant Staphylococcus aureus Cells Adhesion

Detalhes bibliográficos
Autor(a) principal: Guastaldi, Fernando Pozzi Semeghini
Data de Publicação: 2019
Outros Autores: Foggi, Camila Cristina De, Santana, Luis Carlos Leal, Vaz, Luis Geraldo, Vergani, Carlos Eduardo, Guastaldi, Antonio Carlos
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1590/1980-5373-mr-2019-0012
http://hdl.handle.net/11449/183855
Resumo: Extensive data reported the influence of the physicochemical properties on the bacterial adhesion in biomaterials, of which surface roughness of titanium (Ti) can dictates methicillin-resistant Staphylococcus aureus (MRSA) adhesion to orthopedic implants. Herein, we investigated the influence of the Yb:YAG laser texturing of titanium-15molybdenum (TiMo-L) surface on the MRSA (ATCC #33591) cells adhesion and viability. The physicochemical properties and antibacterial performance of TiMo-L were compared to samples of laser-irradiated pure titanium (Ti-L). Polished samples (Ti-P and TiMo-P) were used as controls. Laser textured surfaces presented a high degree of hydrophilicity, an irregular-shaped cavity and a typical microstructured pattern, compared to the polished substrates. The laser irradiation reduced the peaks of molybdenum (Mo) in the surface of Ti-15Mo alloy, which is explained, at least in part, by the difference between the melting point of Ti (1.668 ºC) and Mo (2.623 ºC). Laser texturing raised the MRSA cells viability and statistically increased the bacterial adhesion to pure Ti (P < 0.01; Wilcoxon-Signed rank test) and Ti-15Mo alloy (P < 0.001; Paired t test). The TiMo-L surface was significantly less susceptible to MRSA cell adhesion compared to Ti-L substrate (P < 0.001; Paired t test).
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spelling Lower Susceptibility of Laser-irradiated Ti-15Mo Surface to Methicillin-resistant Staphylococcus aureus Cells AdhesionBiological adhesionmethicillin-resistant Staphylococcus aureussurface treatmenttitanium and alloysExtensive data reported the influence of the physicochemical properties on the bacterial adhesion in biomaterials, of which surface roughness of titanium (Ti) can dictates methicillin-resistant Staphylococcus aureus (MRSA) adhesion to orthopedic implants. Herein, we investigated the influence of the Yb:YAG laser texturing of titanium-15molybdenum (TiMo-L) surface on the MRSA (ATCC #33591) cells adhesion and viability. The physicochemical properties and antibacterial performance of TiMo-L were compared to samples of laser-irradiated pure titanium (Ti-L). Polished samples (Ti-P and TiMo-P) were used as controls. Laser textured surfaces presented a high degree of hydrophilicity, an irregular-shaped cavity and a typical microstructured pattern, compared to the polished substrates. The laser irradiation reduced the peaks of molybdenum (Mo) in the surface of Ti-15Mo alloy, which is explained, at least in part, by the difference between the melting point of Ti (1.668 ºC) and Mo (2.623 ºC). Laser texturing raised the MRSA cells viability and statistically increased the bacterial adhesion to pure Ti (P < 0.01; Wilcoxon-Signed rank test) and Ti-15Mo alloy (P < 0.001; Paired t test). The TiMo-L surface was significantly less susceptible to MRSA cell adhesion compared to Ti-L substrate (P < 0.001; Paired t test).Universidade Estadual Paulista Faculdade de Odontologia de Araraquara Departamento de Diagnóstico e CirurgiaUniversidade Estadual Paulista Faculdade de Odontologia de Araraquara Departamento de Materiais Dentários e PróteseUniversidade Estadual Paulista Instituto de Química de Araraquara Departamento de Físico-QuímicaUniversidade Estadual Paulista Faculdade de Odontologia de Araraquara Departamento de Diagnóstico e CirurgiaUniversidade Estadual Paulista Faculdade de Odontologia de Araraquara Departamento de Materiais Dentários e PróteseUniversidade Estadual Paulista Instituto de Química de Araraquara Departamento de Físico-QuímicaABM, ABC, ABPolUniversidade Estadual Paulista (Unesp)Guastaldi, Fernando Pozzi SemeghiniFoggi, Camila Cristina DeSantana, Luis Carlos LealVaz, Luis GeraldoVergani, Carlos EduardoGuastaldi, Antonio Carlos2019-10-03T17:32:00Z2019-10-03T17:32:00Z2019info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article-application/pdfhttp://dx.doi.org/10.1590/1980-5373-mr-2019-0012Materials Research. ABM, ABC, ABPol, v. 22, n. 4, p. -, 2019.1516-1439http://hdl.handle.net/11449/18385510.1590/1980-5373-mr-2019-0012S1516-14392019000400234S1516-14392019000400234.pdf8547747556446020SciELOreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengMaterials Researchinfo:eu-repo/semantics/openAccess2023-10-26T06:09:22Zoai:repositorio.unesp.br:11449/183855Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462023-10-26T06:09:22Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Lower Susceptibility of Laser-irradiated Ti-15Mo Surface to Methicillin-resistant Staphylococcus aureus Cells Adhesion
title Lower Susceptibility of Laser-irradiated Ti-15Mo Surface to Methicillin-resistant Staphylococcus aureus Cells Adhesion
spellingShingle Lower Susceptibility of Laser-irradiated Ti-15Mo Surface to Methicillin-resistant Staphylococcus aureus Cells Adhesion
Guastaldi, Fernando Pozzi Semeghini
Biological adhesion
methicillin-resistant Staphylococcus aureus
surface treatment
titanium and alloys
title_short Lower Susceptibility of Laser-irradiated Ti-15Mo Surface to Methicillin-resistant Staphylococcus aureus Cells Adhesion
title_full Lower Susceptibility of Laser-irradiated Ti-15Mo Surface to Methicillin-resistant Staphylococcus aureus Cells Adhesion
title_fullStr Lower Susceptibility of Laser-irradiated Ti-15Mo Surface to Methicillin-resistant Staphylococcus aureus Cells Adhesion
title_full_unstemmed Lower Susceptibility of Laser-irradiated Ti-15Mo Surface to Methicillin-resistant Staphylococcus aureus Cells Adhesion
title_sort Lower Susceptibility of Laser-irradiated Ti-15Mo Surface to Methicillin-resistant Staphylococcus aureus Cells Adhesion
author Guastaldi, Fernando Pozzi Semeghini
author_facet Guastaldi, Fernando Pozzi Semeghini
Foggi, Camila Cristina De
Santana, Luis Carlos Leal
Vaz, Luis Geraldo
Vergani, Carlos Eduardo
Guastaldi, Antonio Carlos
author_role author
author2 Foggi, Camila Cristina De
Santana, Luis Carlos Leal
Vaz, Luis Geraldo
Vergani, Carlos Eduardo
Guastaldi, Antonio Carlos
author2_role author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade Estadual Paulista (Unesp)
dc.contributor.author.fl_str_mv Guastaldi, Fernando Pozzi Semeghini
Foggi, Camila Cristina De
Santana, Luis Carlos Leal
Vaz, Luis Geraldo
Vergani, Carlos Eduardo
Guastaldi, Antonio Carlos
dc.subject.por.fl_str_mv Biological adhesion
methicillin-resistant Staphylococcus aureus
surface treatment
titanium and alloys
topic Biological adhesion
methicillin-resistant Staphylococcus aureus
surface treatment
titanium and alloys
description Extensive data reported the influence of the physicochemical properties on the bacterial adhesion in biomaterials, of which surface roughness of titanium (Ti) can dictates methicillin-resistant Staphylococcus aureus (MRSA) adhesion to orthopedic implants. Herein, we investigated the influence of the Yb:YAG laser texturing of titanium-15molybdenum (TiMo-L) surface on the MRSA (ATCC #33591) cells adhesion and viability. The physicochemical properties and antibacterial performance of TiMo-L were compared to samples of laser-irradiated pure titanium (Ti-L). Polished samples (Ti-P and TiMo-P) were used as controls. Laser textured surfaces presented a high degree of hydrophilicity, an irregular-shaped cavity and a typical microstructured pattern, compared to the polished substrates. The laser irradiation reduced the peaks of molybdenum (Mo) in the surface of Ti-15Mo alloy, which is explained, at least in part, by the difference between the melting point of Ti (1.668 ºC) and Mo (2.623 ºC). Laser texturing raised the MRSA cells viability and statistically increased the bacterial adhesion to pure Ti (P < 0.01; Wilcoxon-Signed rank test) and Ti-15Mo alloy (P < 0.001; Paired t test). The TiMo-L surface was significantly less susceptible to MRSA cell adhesion compared to Ti-L substrate (P < 0.001; Paired t test).
publishDate 2019
dc.date.none.fl_str_mv 2019-10-03T17:32:00Z
2019-10-03T17:32:00Z
2019
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1590/1980-5373-mr-2019-0012
Materials Research. ABM, ABC, ABPol, v. 22, n. 4, p. -, 2019.
1516-1439
http://hdl.handle.net/11449/183855
10.1590/1980-5373-mr-2019-0012
S1516-14392019000400234
S1516-14392019000400234.pdf
8547747556446020
url http://dx.doi.org/10.1590/1980-5373-mr-2019-0012
http://hdl.handle.net/11449/183855
identifier_str_mv Materials Research. ABM, ABC, ABPol, v. 22, n. 4, p. -, 2019.
1516-1439
10.1590/1980-5373-mr-2019-0012
S1516-14392019000400234
S1516-14392019000400234.pdf
8547747556446020
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Materials Research
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv -
application/pdf
dc.publisher.none.fl_str_mv ABM, ABC, ABPol
publisher.none.fl_str_mv ABM, ABC, ABPol
dc.source.none.fl_str_mv SciELO
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
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