Swine as reservoirs of zoonotic borderline oxacillin-resistant Staphylococcus aureus ST398

Detalhes bibliográficos
Autor(a) principal: Santos, S. C.L.
Data de Publicação: 2021
Outros Autores: Saraiva, M. M.S. [UNESP], Moreira Filho, A. L.B., Silva, N. M.V., De Leon, C. M.G., Pascoal, L. A.F., Givisiez, P. E.N., Gebreyes, W. A., Oliveira, C. J.B.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1016/j.cimid.2021.101697
http://hdl.handle.net/11449/222395
Resumo: Methicillin resistance mediated by the mecA gene in Staphylococcus aureus, also known as “true MRSA”, is typically associated with high oxacillin MIC values (≥8 mg/L). Because non-mecA-mediated oxacillin resistant S. aureus phenotypes can also cause hard-to-treat diseases in humans, their misidentification as methicillin-susceptible S. aureus strains (MSSA) can compromise the efficiency of the antimicrobial therapy. These strains have been refereed as Borderline Oxacillin-Resistant S. aureus (BORSA) but their characterization and role in clinical microbiology have been neglected. Considering the increasing importance of livestock-associated methicillin-resistant S. aureus ST398 (LA-MRSA) as an emerging zoonotic pathogen worldwide, this study aimed to report the genomic context of oxacillin resistance in porcine S. aureus ST398 strains. S. aureus isolates were recovered from asymptomatic pigs from three herds. Oxacillin MIC values ranged from 4 to 32 mg/L. MALDI-TOF-confirmed isolates were screened for mecA and mecC by PCR and genotyped by means of PFGE and Rep-PCR. Seven isolates were whole genome sequenced. None of the isolates harbored the mecA gene or its variants. Although all seven sequenced isolates belonged to one sequence type (ST398), two different spa types (t571 and t1471) were identified. All isolates harbored conserved blaZ gene operon and no mutations on genes encoding for penicillin-binding-proteins were detected. Genes conferring resistance against other drugs such as aminoglycosides, chloramphenicol, macrolide, lincosamide and streptogramin (MLS), tetracycline and trimethoprim were also detected. Isolates also harbored virulence genes encoding for adhesins (icaA; icaB; icaC; icaD; icaR), toxins (hlgA; hlgB; hlgC; luk-PV) and protease (aur). Pigs can serve as reservoirs of non-mecA-mediated oxacillin-resistant ST398 strains potentially pathogenic to humans. Considering that mecA has been the main target to screen methicillin-resistant staphylococci, the occurrence of BORSA phenotypes is probably underestimated in livestock.
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spelling Swine as reservoirs of zoonotic borderline oxacillin-resistant Staphylococcus aureus ST398Antimcirobial resistanceblaZmecMethicillinPigsMethicillin resistance mediated by the mecA gene in Staphylococcus aureus, also known as “true MRSA”, is typically associated with high oxacillin MIC values (≥8 mg/L). Because non-mecA-mediated oxacillin resistant S. aureus phenotypes can also cause hard-to-treat diseases in humans, their misidentification as methicillin-susceptible S. aureus strains (MSSA) can compromise the efficiency of the antimicrobial therapy. These strains have been refereed as Borderline Oxacillin-Resistant S. aureus (BORSA) but their characterization and role in clinical microbiology have been neglected. Considering the increasing importance of livestock-associated methicillin-resistant S. aureus ST398 (LA-MRSA) as an emerging zoonotic pathogen worldwide, this study aimed to report the genomic context of oxacillin resistance in porcine S. aureus ST398 strains. S. aureus isolates were recovered from asymptomatic pigs from three herds. Oxacillin MIC values ranged from 4 to 32 mg/L. MALDI-TOF-confirmed isolates were screened for mecA and mecC by PCR and genotyped by means of PFGE and Rep-PCR. Seven isolates were whole genome sequenced. None of the isolates harbored the mecA gene or its variants. Although all seven sequenced isolates belonged to one sequence type (ST398), two different spa types (t571 and t1471) were identified. All isolates harbored conserved blaZ gene operon and no mutations on genes encoding for penicillin-binding-proteins were detected. Genes conferring resistance against other drugs such as aminoglycosides, chloramphenicol, macrolide, lincosamide and streptogramin (MLS), tetracycline and trimethoprim were also detected. Isolates also harbored virulence genes encoding for adhesins (icaA; icaB; icaC; icaD; icaR), toxins (hlgA; hlgB; hlgC; luk-PV) and protease (aur). Pigs can serve as reservoirs of non-mecA-mediated oxacillin-resistant ST398 strains potentially pathogenic to humans. Considering that mecA has been the main target to screen methicillin-resistant staphylococci, the occurrence of BORSA phenotypes is probably underestimated in livestock.Financiadora de Estudos e ProjetosDepartment of Animal Science College for Agricultural Sciences Federal University of Paraiba (UFPB), Rod. PB079 Km12, s/nDepartment of Veterinary Pathology Sao Paulo State University (UNESP), Via de acesso Prof. Paulo Donato Castellane s/nDepartment of Animal Science College for Agricultural Social and Human Sciences Federal University of Paraiba (UFPB), Rua João Pessoa s/nDepartment of Veterinary Preventive Medicine College of Veterinary Medicine The Ohio State University (OSU)Global One Health Initiative (GOHi) The Ohio State UniversityDepartment of Veterinary Pathology Sao Paulo State University (UNESP), Via de acesso Prof. Paulo Donato Castellane s/nUniversidade Federal da Paraíba (UFPB)Universidade Estadual Paulista (UNESP)The Ohio State University (OSU)The Ohio State UniversitySantos, S. C.L.Saraiva, M. M.S. [UNESP]Moreira Filho, A. L.B.Silva, N. M.V.De Leon, C. M.G.Pascoal, L. A.F.Givisiez, P. E.N.Gebreyes, W. A.Oliveira, C. J.B.2022-04-28T19:44:22Z2022-04-28T19:44:22Z2021-12-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.1016/j.cimid.2021.101697Comparative Immunology, Microbiology and Infectious Diseases, v. 79.1878-16670147-9571http://hdl.handle.net/11449/22239510.1016/j.cimid.2021.1016972-s2.0-85114750539Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengComparative Immunology, Microbiology and Infectious Diseasesinfo:eu-repo/semantics/openAccess2022-04-28T19:44:22Zoai:repositorio.unesp.br:11449/222395Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T17:34:40.183475Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Swine as reservoirs of zoonotic borderline oxacillin-resistant Staphylococcus aureus ST398
title Swine as reservoirs of zoonotic borderline oxacillin-resistant Staphylococcus aureus ST398
spellingShingle Swine as reservoirs of zoonotic borderline oxacillin-resistant Staphylococcus aureus ST398
Santos, S. C.L.
Antimcirobial resistance
blaZ
mec
Methicillin
Pigs
title_short Swine as reservoirs of zoonotic borderline oxacillin-resistant Staphylococcus aureus ST398
title_full Swine as reservoirs of zoonotic borderline oxacillin-resistant Staphylococcus aureus ST398
title_fullStr Swine as reservoirs of zoonotic borderline oxacillin-resistant Staphylococcus aureus ST398
title_full_unstemmed Swine as reservoirs of zoonotic borderline oxacillin-resistant Staphylococcus aureus ST398
title_sort Swine as reservoirs of zoonotic borderline oxacillin-resistant Staphylococcus aureus ST398
author Santos, S. C.L.
author_facet Santos, S. C.L.
Saraiva, M. M.S. [UNESP]
Moreira Filho, A. L.B.
Silva, N. M.V.
De Leon, C. M.G.
Pascoal, L. A.F.
Givisiez, P. E.N.
Gebreyes, W. A.
Oliveira, C. J.B.
author_role author
author2 Saraiva, M. M.S. [UNESP]
Moreira Filho, A. L.B.
Silva, N. M.V.
De Leon, C. M.G.
Pascoal, L. A.F.
Givisiez, P. E.N.
Gebreyes, W. A.
Oliveira, C. J.B.
author2_role author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade Federal da Paraíba (UFPB)
Universidade Estadual Paulista (UNESP)
The Ohio State University (OSU)
The Ohio State University
dc.contributor.author.fl_str_mv Santos, S. C.L.
Saraiva, M. M.S. [UNESP]
Moreira Filho, A. L.B.
Silva, N. M.V.
De Leon, C. M.G.
Pascoal, L. A.F.
Givisiez, P. E.N.
Gebreyes, W. A.
Oliveira, C. J.B.
dc.subject.por.fl_str_mv Antimcirobial resistance
blaZ
mec
Methicillin
Pigs
topic Antimcirobial resistance
blaZ
mec
Methicillin
Pigs
description Methicillin resistance mediated by the mecA gene in Staphylococcus aureus, also known as “true MRSA”, is typically associated with high oxacillin MIC values (≥8 mg/L). Because non-mecA-mediated oxacillin resistant S. aureus phenotypes can also cause hard-to-treat diseases in humans, their misidentification as methicillin-susceptible S. aureus strains (MSSA) can compromise the efficiency of the antimicrobial therapy. These strains have been refereed as Borderline Oxacillin-Resistant S. aureus (BORSA) but their characterization and role in clinical microbiology have been neglected. Considering the increasing importance of livestock-associated methicillin-resistant S. aureus ST398 (LA-MRSA) as an emerging zoonotic pathogen worldwide, this study aimed to report the genomic context of oxacillin resistance in porcine S. aureus ST398 strains. S. aureus isolates were recovered from asymptomatic pigs from three herds. Oxacillin MIC values ranged from 4 to 32 mg/L. MALDI-TOF-confirmed isolates were screened for mecA and mecC by PCR and genotyped by means of PFGE and Rep-PCR. Seven isolates were whole genome sequenced. None of the isolates harbored the mecA gene or its variants. Although all seven sequenced isolates belonged to one sequence type (ST398), two different spa types (t571 and t1471) were identified. All isolates harbored conserved blaZ gene operon and no mutations on genes encoding for penicillin-binding-proteins were detected. Genes conferring resistance against other drugs such as aminoglycosides, chloramphenicol, macrolide, lincosamide and streptogramin (MLS), tetracycline and trimethoprim were also detected. Isolates also harbored virulence genes encoding for adhesins (icaA; icaB; icaC; icaD; icaR), toxins (hlgA; hlgB; hlgC; luk-PV) and protease (aur). Pigs can serve as reservoirs of non-mecA-mediated oxacillin-resistant ST398 strains potentially pathogenic to humans. Considering that mecA has been the main target to screen methicillin-resistant staphylococci, the occurrence of BORSA phenotypes is probably underestimated in livestock.
publishDate 2021
dc.date.none.fl_str_mv 2021-12-01
2022-04-28T19:44:22Z
2022-04-28T19:44:22Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1016/j.cimid.2021.101697
Comparative Immunology, Microbiology and Infectious Diseases, v. 79.
1878-1667
0147-9571
http://hdl.handle.net/11449/222395
10.1016/j.cimid.2021.101697
2-s2.0-85114750539
url http://dx.doi.org/10.1016/j.cimid.2021.101697
http://hdl.handle.net/11449/222395
identifier_str_mv Comparative Immunology, Microbiology and Infectious Diseases, v. 79.
1878-1667
0147-9571
10.1016/j.cimid.2021.101697
2-s2.0-85114750539
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Comparative Immunology, Microbiology and Infectious Diseases
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
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