Swine as reservoirs of zoonotic borderline oxacillin-resistant Staphylococcus aureus ST398
Autor(a) principal: | |
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Data de Publicação: | 2021 |
Outros Autores: | , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1016/j.cimid.2021.101697 http://hdl.handle.net/11449/222395 |
Resumo: | Methicillin resistance mediated by the mecA gene in Staphylococcus aureus, also known as “true MRSA”, is typically associated with high oxacillin MIC values (≥8 mg/L). Because non-mecA-mediated oxacillin resistant S. aureus phenotypes can also cause hard-to-treat diseases in humans, their misidentification as methicillin-susceptible S. aureus strains (MSSA) can compromise the efficiency of the antimicrobial therapy. These strains have been refereed as Borderline Oxacillin-Resistant S. aureus (BORSA) but their characterization and role in clinical microbiology have been neglected. Considering the increasing importance of livestock-associated methicillin-resistant S. aureus ST398 (LA-MRSA) as an emerging zoonotic pathogen worldwide, this study aimed to report the genomic context of oxacillin resistance in porcine S. aureus ST398 strains. S. aureus isolates were recovered from asymptomatic pigs from three herds. Oxacillin MIC values ranged from 4 to 32 mg/L. MALDI-TOF-confirmed isolates were screened for mecA and mecC by PCR and genotyped by means of PFGE and Rep-PCR. Seven isolates were whole genome sequenced. None of the isolates harbored the mecA gene or its variants. Although all seven sequenced isolates belonged to one sequence type (ST398), two different spa types (t571 and t1471) were identified. All isolates harbored conserved blaZ gene operon and no mutations on genes encoding for penicillin-binding-proteins were detected. Genes conferring resistance against other drugs such as aminoglycosides, chloramphenicol, macrolide, lincosamide and streptogramin (MLS), tetracycline and trimethoprim were also detected. Isolates also harbored virulence genes encoding for adhesins (icaA; icaB; icaC; icaD; icaR), toxins (hlgA; hlgB; hlgC; luk-PV) and protease (aur). Pigs can serve as reservoirs of non-mecA-mediated oxacillin-resistant ST398 strains potentially pathogenic to humans. Considering that mecA has been the main target to screen methicillin-resistant staphylococci, the occurrence of BORSA phenotypes is probably underestimated in livestock. |
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Swine as reservoirs of zoonotic borderline oxacillin-resistant Staphylococcus aureus ST398Antimcirobial resistanceblaZmecMethicillinPigsMethicillin resistance mediated by the mecA gene in Staphylococcus aureus, also known as “true MRSA”, is typically associated with high oxacillin MIC values (≥8 mg/L). Because non-mecA-mediated oxacillin resistant S. aureus phenotypes can also cause hard-to-treat diseases in humans, their misidentification as methicillin-susceptible S. aureus strains (MSSA) can compromise the efficiency of the antimicrobial therapy. These strains have been refereed as Borderline Oxacillin-Resistant S. aureus (BORSA) but their characterization and role in clinical microbiology have been neglected. Considering the increasing importance of livestock-associated methicillin-resistant S. aureus ST398 (LA-MRSA) as an emerging zoonotic pathogen worldwide, this study aimed to report the genomic context of oxacillin resistance in porcine S. aureus ST398 strains. S. aureus isolates were recovered from asymptomatic pigs from three herds. Oxacillin MIC values ranged from 4 to 32 mg/L. MALDI-TOF-confirmed isolates were screened for mecA and mecC by PCR and genotyped by means of PFGE and Rep-PCR. Seven isolates were whole genome sequenced. None of the isolates harbored the mecA gene or its variants. Although all seven sequenced isolates belonged to one sequence type (ST398), two different spa types (t571 and t1471) were identified. All isolates harbored conserved blaZ gene operon and no mutations on genes encoding for penicillin-binding-proteins were detected. Genes conferring resistance against other drugs such as aminoglycosides, chloramphenicol, macrolide, lincosamide and streptogramin (MLS), tetracycline and trimethoprim were also detected. Isolates also harbored virulence genes encoding for adhesins (icaA; icaB; icaC; icaD; icaR), toxins (hlgA; hlgB; hlgC; luk-PV) and protease (aur). Pigs can serve as reservoirs of non-mecA-mediated oxacillin-resistant ST398 strains potentially pathogenic to humans. Considering that mecA has been the main target to screen methicillin-resistant staphylococci, the occurrence of BORSA phenotypes is probably underestimated in livestock.Financiadora de Estudos e ProjetosDepartment of Animal Science College for Agricultural Sciences Federal University of Paraiba (UFPB), Rod. PB079 Km12, s/nDepartment of Veterinary Pathology Sao Paulo State University (UNESP), Via de acesso Prof. Paulo Donato Castellane s/nDepartment of Animal Science College for Agricultural Social and Human Sciences Federal University of Paraiba (UFPB), Rua João Pessoa s/nDepartment of Veterinary Preventive Medicine College of Veterinary Medicine The Ohio State University (OSU)Global One Health Initiative (GOHi) The Ohio State UniversityDepartment of Veterinary Pathology Sao Paulo State University (UNESP), Via de acesso Prof. Paulo Donato Castellane s/nUniversidade Federal da Paraíba (UFPB)Universidade Estadual Paulista (UNESP)The Ohio State University (OSU)The Ohio State UniversitySantos, S. C.L.Saraiva, M. M.S. [UNESP]Moreira Filho, A. L.B.Silva, N. M.V.De Leon, C. M.G.Pascoal, L. A.F.Givisiez, P. E.N.Gebreyes, W. A.Oliveira, C. J.B.2022-04-28T19:44:22Z2022-04-28T19:44:22Z2021-12-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.1016/j.cimid.2021.101697Comparative Immunology, Microbiology and Infectious Diseases, v. 79.1878-16670147-9571http://hdl.handle.net/11449/22239510.1016/j.cimid.2021.1016972-s2.0-85114750539Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengComparative Immunology, Microbiology and Infectious Diseasesinfo:eu-repo/semantics/openAccess2022-04-28T19:44:22Zoai:repositorio.unesp.br:11449/222395Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T17:34:40.183475Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Swine as reservoirs of zoonotic borderline oxacillin-resistant Staphylococcus aureus ST398 |
title |
Swine as reservoirs of zoonotic borderline oxacillin-resistant Staphylococcus aureus ST398 |
spellingShingle |
Swine as reservoirs of zoonotic borderline oxacillin-resistant Staphylococcus aureus ST398 Santos, S. C.L. Antimcirobial resistance blaZ mec Methicillin Pigs |
title_short |
Swine as reservoirs of zoonotic borderline oxacillin-resistant Staphylococcus aureus ST398 |
title_full |
Swine as reservoirs of zoonotic borderline oxacillin-resistant Staphylococcus aureus ST398 |
title_fullStr |
Swine as reservoirs of zoonotic borderline oxacillin-resistant Staphylococcus aureus ST398 |
title_full_unstemmed |
Swine as reservoirs of zoonotic borderline oxacillin-resistant Staphylococcus aureus ST398 |
title_sort |
Swine as reservoirs of zoonotic borderline oxacillin-resistant Staphylococcus aureus ST398 |
author |
Santos, S. C.L. |
author_facet |
Santos, S. C.L. Saraiva, M. M.S. [UNESP] Moreira Filho, A. L.B. Silva, N. M.V. De Leon, C. M.G. Pascoal, L. A.F. Givisiez, P. E.N. Gebreyes, W. A. Oliveira, C. J.B. |
author_role |
author |
author2 |
Saraiva, M. M.S. [UNESP] Moreira Filho, A. L.B. Silva, N. M.V. De Leon, C. M.G. Pascoal, L. A.F. Givisiez, P. E.N. Gebreyes, W. A. Oliveira, C. J.B. |
author2_role |
author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Federal da Paraíba (UFPB) Universidade Estadual Paulista (UNESP) The Ohio State University (OSU) The Ohio State University |
dc.contributor.author.fl_str_mv |
Santos, S. C.L. Saraiva, M. M.S. [UNESP] Moreira Filho, A. L.B. Silva, N. M.V. De Leon, C. M.G. Pascoal, L. A.F. Givisiez, P. E.N. Gebreyes, W. A. Oliveira, C. J.B. |
dc.subject.por.fl_str_mv |
Antimcirobial resistance blaZ mec Methicillin Pigs |
topic |
Antimcirobial resistance blaZ mec Methicillin Pigs |
description |
Methicillin resistance mediated by the mecA gene in Staphylococcus aureus, also known as “true MRSA”, is typically associated with high oxacillin MIC values (≥8 mg/L). Because non-mecA-mediated oxacillin resistant S. aureus phenotypes can also cause hard-to-treat diseases in humans, their misidentification as methicillin-susceptible S. aureus strains (MSSA) can compromise the efficiency of the antimicrobial therapy. These strains have been refereed as Borderline Oxacillin-Resistant S. aureus (BORSA) but their characterization and role in clinical microbiology have been neglected. Considering the increasing importance of livestock-associated methicillin-resistant S. aureus ST398 (LA-MRSA) as an emerging zoonotic pathogen worldwide, this study aimed to report the genomic context of oxacillin resistance in porcine S. aureus ST398 strains. S. aureus isolates were recovered from asymptomatic pigs from three herds. Oxacillin MIC values ranged from 4 to 32 mg/L. MALDI-TOF-confirmed isolates were screened for mecA and mecC by PCR and genotyped by means of PFGE and Rep-PCR. Seven isolates were whole genome sequenced. None of the isolates harbored the mecA gene or its variants. Although all seven sequenced isolates belonged to one sequence type (ST398), two different spa types (t571 and t1471) were identified. All isolates harbored conserved blaZ gene operon and no mutations on genes encoding for penicillin-binding-proteins were detected. Genes conferring resistance against other drugs such as aminoglycosides, chloramphenicol, macrolide, lincosamide and streptogramin (MLS), tetracycline and trimethoprim were also detected. Isolates also harbored virulence genes encoding for adhesins (icaA; icaB; icaC; icaD; icaR), toxins (hlgA; hlgB; hlgC; luk-PV) and protease (aur). Pigs can serve as reservoirs of non-mecA-mediated oxacillin-resistant ST398 strains potentially pathogenic to humans. Considering that mecA has been the main target to screen methicillin-resistant staphylococci, the occurrence of BORSA phenotypes is probably underestimated in livestock. |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021-12-01 2022-04-28T19:44:22Z 2022-04-28T19:44:22Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1016/j.cimid.2021.101697 Comparative Immunology, Microbiology and Infectious Diseases, v. 79. 1878-1667 0147-9571 http://hdl.handle.net/11449/222395 10.1016/j.cimid.2021.101697 2-s2.0-85114750539 |
url |
http://dx.doi.org/10.1016/j.cimid.2021.101697 http://hdl.handle.net/11449/222395 |
identifier_str_mv |
Comparative Immunology, Microbiology and Infectious Diseases, v. 79. 1878-1667 0147-9571 10.1016/j.cimid.2021.101697 2-s2.0-85114750539 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Comparative Immunology, Microbiology and Infectious Diseases |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
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1808128828966436864 |