Effect of aging on periodontal inflammation, microbial colonization, and disease susceptibility
Autor(a) principal: | |
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Data de Publicação: | 2015 |
Outros Autores: | , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1177/0022034515625962 http://hdl.handle.net/11449/172731 |
Resumo: | Periodontitis is a chronic inflammatory disease induced by a biofilm that forms on the tooth surface. Increased periodontal disease is associated with aging. We investigated the effect of aging on challenge by oral pathogens, examining the host response, colonization, and osteoclast numbers in aged versus young mice. We also compared the results with mice with lineage-specific deletion of the transcription factor FOXO1, which reduces dendritic cell (DC) function. Periodontitis was induced by oral inoculation of Porphyromonas gingivalis and Fusobacterium nucleatum in young (4 to 5 mo) and aged (14 to 15 mo) mice. Aged mice as well as mice with reduced DC function had decreased numbers of DCs in lymph nodes, indicative of a diminished host response. In vitro studies suggest that reduced DC numbers in lymph nodes of aged mice may involve the effect of advanced glycation end products on DC migration. Surprisingly, aged mice but not mice with genetically altered DC function had greater production of antibody to P. gingivalis, greater IL-12 expression, and more plasma cells in lymph nodes following oral inoculation as compared with young mice. The greater adaptive immune response in aged versus young mice was linked to enhanced levels of P. gingivalis and reduced bacterial diversity. Thus, reduced bacterial diversity in aged mice may contribute to increased P. gingivalis colonization following inoculation and increased periodontal disease susceptibility, reflected by higher TNF levels and osteoclast numbers in the periodontium of aged versus young mice. |
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Effect of aging on periodontal inflammation, microbial colonization, and disease susceptibilitybacteriadendritic cellDNA-seqlymphocyteosteoclastperiodontitisPeriodontitis is a chronic inflammatory disease induced by a biofilm that forms on the tooth surface. Increased periodontal disease is associated with aging. We investigated the effect of aging on challenge by oral pathogens, examining the host response, colonization, and osteoclast numbers in aged versus young mice. We also compared the results with mice with lineage-specific deletion of the transcription factor FOXO1, which reduces dendritic cell (DC) function. Periodontitis was induced by oral inoculation of Porphyromonas gingivalis and Fusobacterium nucleatum in young (4 to 5 mo) and aged (14 to 15 mo) mice. Aged mice as well as mice with reduced DC function had decreased numbers of DCs in lymph nodes, indicative of a diminished host response. In vitro studies suggest that reduced DC numbers in lymph nodes of aged mice may involve the effect of advanced glycation end products on DC migration. Surprisingly, aged mice but not mice with genetically altered DC function had greater production of antibody to P. gingivalis, greater IL-12 expression, and more plasma cells in lymph nodes following oral inoculation as compared with young mice. The greater adaptive immune response in aged versus young mice was linked to enhanced levels of P. gingivalis and reduced bacterial diversity. Thus, reduced bacterial diversity in aged mice may contribute to increased P. gingivalis colonization following inoculation and increased periodontal disease susceptibility, reflected by higher TNF levels and osteoclast numbers in the periodontium of aged versus young mice.National Institute of Dental and Craniofacial ResearchState Key Laboratory of Oral Disease West China Hospital of Stomatology Sichuan UniversityDepartment of Periodontics School of Dental Medicine University of Pennsylvania, 240 South 40th StreetDepartment of Periodontology Peking University School and Hospital of StomatologyDepartment of Oral and Maxillofacial Surgery Peking University School and Hospital of StomatologyShanxi Province People's HospitalDepartment of Diagnosis and Surgery School of Dentistry at Araraquara-UNESPDepartment of Diagnosis and Surgery School of Dentistry at Araraquara-UNESPNational Institute of Dental and Craniofacial Research: P30AR050950National Institute of Dental and Craniofacial Research: R01-DE021921Sichuan UniversityUniversity of PennsylvaniaPeking University School and Hospital of StomatologyShanxi Province People's HospitalUniversidade Estadual Paulista (Unesp)Wu, Y.Dong, G.Xiao, W.Xiao, E.Miao, F.Syverson, A.Missaghian, N.Vafa, R.Cabrera-Ortega, A. A. [UNESP]Rossa, C. [UNESP]Graves, D. T.2018-12-11T17:01:57Z2018-12-11T17:01:57Z2015-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article460-466application/pdfhttp://dx.doi.org/10.1177/0022034515625962Journal of Dental Research, v. 95, n. 4, p. 460-466, 2015.1544-05910022-0345http://hdl.handle.net/11449/17273110.1177/00220345156259622-s2.0-849616370842-s2.0-84961637084.pdfScopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengJournal of Dental Research2,302info:eu-repo/semantics/openAccess2023-11-15T06:16:22Zoai:repositorio.unesp.br:11449/172731Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462023-11-15T06:16:22Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Effect of aging on periodontal inflammation, microbial colonization, and disease susceptibility |
title |
Effect of aging on periodontal inflammation, microbial colonization, and disease susceptibility |
spellingShingle |
Effect of aging on periodontal inflammation, microbial colonization, and disease susceptibility Wu, Y. bacteria dendritic cell DNA-seq lymphocyte osteoclast periodontitis |
title_short |
Effect of aging on periodontal inflammation, microbial colonization, and disease susceptibility |
title_full |
Effect of aging on periodontal inflammation, microbial colonization, and disease susceptibility |
title_fullStr |
Effect of aging on periodontal inflammation, microbial colonization, and disease susceptibility |
title_full_unstemmed |
Effect of aging on periodontal inflammation, microbial colonization, and disease susceptibility |
title_sort |
Effect of aging on periodontal inflammation, microbial colonization, and disease susceptibility |
author |
Wu, Y. |
author_facet |
Wu, Y. Dong, G. Xiao, W. Xiao, E. Miao, F. Syverson, A. Missaghian, N. Vafa, R. Cabrera-Ortega, A. A. [UNESP] Rossa, C. [UNESP] Graves, D. T. |
author_role |
author |
author2 |
Dong, G. Xiao, W. Xiao, E. Miao, F. Syverson, A. Missaghian, N. Vafa, R. Cabrera-Ortega, A. A. [UNESP] Rossa, C. [UNESP] Graves, D. T. |
author2_role |
author author author author author author author author author author |
dc.contributor.none.fl_str_mv |
Sichuan University University of Pennsylvania Peking University School and Hospital of Stomatology Shanxi Province People's Hospital Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Wu, Y. Dong, G. Xiao, W. Xiao, E. Miao, F. Syverson, A. Missaghian, N. Vafa, R. Cabrera-Ortega, A. A. [UNESP] Rossa, C. [UNESP] Graves, D. T. |
dc.subject.por.fl_str_mv |
bacteria dendritic cell DNA-seq lymphocyte osteoclast periodontitis |
topic |
bacteria dendritic cell DNA-seq lymphocyte osteoclast periodontitis |
description |
Periodontitis is a chronic inflammatory disease induced by a biofilm that forms on the tooth surface. Increased periodontal disease is associated with aging. We investigated the effect of aging on challenge by oral pathogens, examining the host response, colonization, and osteoclast numbers in aged versus young mice. We also compared the results with mice with lineage-specific deletion of the transcription factor FOXO1, which reduces dendritic cell (DC) function. Periodontitis was induced by oral inoculation of Porphyromonas gingivalis and Fusobacterium nucleatum in young (4 to 5 mo) and aged (14 to 15 mo) mice. Aged mice as well as mice with reduced DC function had decreased numbers of DCs in lymph nodes, indicative of a diminished host response. In vitro studies suggest that reduced DC numbers in lymph nodes of aged mice may involve the effect of advanced glycation end products on DC migration. Surprisingly, aged mice but not mice with genetically altered DC function had greater production of antibody to P. gingivalis, greater IL-12 expression, and more plasma cells in lymph nodes following oral inoculation as compared with young mice. The greater adaptive immune response in aged versus young mice was linked to enhanced levels of P. gingivalis and reduced bacterial diversity. Thus, reduced bacterial diversity in aged mice may contribute to increased P. gingivalis colonization following inoculation and increased periodontal disease susceptibility, reflected by higher TNF levels and osteoclast numbers in the periodontium of aged versus young mice. |
publishDate |
2015 |
dc.date.none.fl_str_mv |
2015-01-01 2018-12-11T17:01:57Z 2018-12-11T17:01:57Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1177/0022034515625962 Journal of Dental Research, v. 95, n. 4, p. 460-466, 2015. 1544-0591 0022-0345 http://hdl.handle.net/11449/172731 10.1177/0022034515625962 2-s2.0-84961637084 2-s2.0-84961637084.pdf |
url |
http://dx.doi.org/10.1177/0022034515625962 http://hdl.handle.net/11449/172731 |
identifier_str_mv |
Journal of Dental Research, v. 95, n. 4, p. 460-466, 2015. 1544-0591 0022-0345 10.1177/0022034515625962 2-s2.0-84961637084 2-s2.0-84961637084.pdf |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Journal of Dental Research 2,302 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
460-466 application/pdf |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1803649720377147392 |