Proteomic profile of sex-sorted bull sperm evaluated by SWATH-MS analysis
Autor(a) principal: | |
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Data de Publicação: | 2018 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1016/j.anireprosci.2018.09.010 http://hdl.handle.net/11449/188114 |
Resumo: | The identification of distinct proteins present on the membrane of spermatozoa with X and Y chromosomes allows the development of immuno-sexing techniques. The aim of this study, therefore, was to use mass spectrometry to analyze the protein profile of sperm previously categorized using flow cytometry into X or Y-bearing semen pools. Sex-sorted sperm samples (n = 6 X and n = 6 Y) were used. Proteins were extracted and analyzed by mass spectrometry using data independent acquisition (DIA). The data were searched against taxonomy Bos taurus in the Swiss Prot database. In total, 459 protein groups were identified. Of these, eight proteins were in differential abundances between the X- and Y-bearing sperm population. Among the major proteinsdetected, EF-hand domain-containing protein 1, a protein involved in embryonic development, is more abundant in Y-bearing spermatozoa. In addition, proteins FUN14, domain-containing protein 2, NADH dehydrogenase [ubiquinone] iron-sulfur protein 7 mitochondrial, cytochrome C oxidase subunit 2, acetyl -CoA carboxylase type beta were more abundant in X-bearing sperm. In conclusion, there were differences in abundance of proteins between X- and Y-bearing bull spermatozoa. This fact, may contribute to future studies related to sperm physiology and possibility development of immuno-sexing techniques. |
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Proteomic profile of sex-sorted bull sperm evaluated by SWATH-MS analysisBovineImmuno-sexingProteomicsSex-sorted-spermThe identification of distinct proteins present on the membrane of spermatozoa with X and Y chromosomes allows the development of immuno-sexing techniques. The aim of this study, therefore, was to use mass spectrometry to analyze the protein profile of sperm previously categorized using flow cytometry into X or Y-bearing semen pools. Sex-sorted sperm samples (n = 6 X and n = 6 Y) were used. Proteins were extracted and analyzed by mass spectrometry using data independent acquisition (DIA). The data were searched against taxonomy Bos taurus in the Swiss Prot database. In total, 459 protein groups were identified. Of these, eight proteins were in differential abundances between the X- and Y-bearing sperm population. Among the major proteinsdetected, EF-hand domain-containing protein 1, a protein involved in embryonic development, is more abundant in Y-bearing spermatozoa. In addition, proteins FUN14, domain-containing protein 2, NADH dehydrogenase [ubiquinone] iron-sulfur protein 7 mitochondrial, cytochrome C oxidase subunit 2, acetyl -CoA carboxylase type beta were more abundant in X-bearing sperm. In conclusion, there were differences in abundance of proteins between X- and Y-bearing bull spermatozoa. This fact, may contribute to future studies related to sperm physiology and possibility development of immuno-sexing techniques.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)São Paulo State University (UNESP) School of Veterinary Medicine and Animal Science Department of Animal Reproduction and Veterinary RadiologyReproductive Science Group Faculty of Science University of NewcastleAustralian Proteome Analysis Facility (APAF) Department of Chemistry and Biomolecular Sciences Macquarie UniversitySão Paulo State University (UNESP) School of Veterinary Medicine and Animal Science Department of Animal Reproduction and Veterinary RadiologyFAPESP: 2015/25638-3Universidade Estadual Paulista (Unesp)University of NewcastleMacquarie UniversityScott, Caroline [UNESP]de Souza, Fabiana F. [UNESP]Aristizabal, Viviana H.V. [UNESP]Hethrington, LouiseKrisp, ChristophMolloy, MarkBaker, Mark A.Dell'Aqua, José Antônio [UNESP]2019-10-06T15:57:44Z2019-10-06T15:57:44Z2018-11-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article121-128http://dx.doi.org/10.1016/j.anireprosci.2018.09.010Animal Reproduction Science, v. 198, p. 121-128.0378-4320http://hdl.handle.net/11449/18811410.1016/j.anireprosci.2018.09.0102-s2.0-85054001326Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengAnimal Reproduction Scienceinfo:eu-repo/semantics/openAccess2024-09-09T14:01:21Zoai:repositorio.unesp.br:11449/188114Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestrepositoriounesp@unesp.bropendoar:29462024-09-09T14:01:21Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Proteomic profile of sex-sorted bull sperm evaluated by SWATH-MS analysis |
title |
Proteomic profile of sex-sorted bull sperm evaluated by SWATH-MS analysis |
spellingShingle |
Proteomic profile of sex-sorted bull sperm evaluated by SWATH-MS analysis Scott, Caroline [UNESP] Bovine Immuno-sexing Proteomics Sex-sorted-sperm |
title_short |
Proteomic profile of sex-sorted bull sperm evaluated by SWATH-MS analysis |
title_full |
Proteomic profile of sex-sorted bull sperm evaluated by SWATH-MS analysis |
title_fullStr |
Proteomic profile of sex-sorted bull sperm evaluated by SWATH-MS analysis |
title_full_unstemmed |
Proteomic profile of sex-sorted bull sperm evaluated by SWATH-MS analysis |
title_sort |
Proteomic profile of sex-sorted bull sperm evaluated by SWATH-MS analysis |
author |
Scott, Caroline [UNESP] |
author_facet |
Scott, Caroline [UNESP] de Souza, Fabiana F. [UNESP] Aristizabal, Viviana H.V. [UNESP] Hethrington, Louise Krisp, Christoph Molloy, Mark Baker, Mark A. Dell'Aqua, José Antônio [UNESP] |
author_role |
author |
author2 |
de Souza, Fabiana F. [UNESP] Aristizabal, Viviana H.V. [UNESP] Hethrington, Louise Krisp, Christoph Molloy, Mark Baker, Mark A. Dell'Aqua, José Antônio [UNESP] |
author2_role |
author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) University of Newcastle Macquarie University |
dc.contributor.author.fl_str_mv |
Scott, Caroline [UNESP] de Souza, Fabiana F. [UNESP] Aristizabal, Viviana H.V. [UNESP] Hethrington, Louise Krisp, Christoph Molloy, Mark Baker, Mark A. Dell'Aqua, José Antônio [UNESP] |
dc.subject.por.fl_str_mv |
Bovine Immuno-sexing Proteomics Sex-sorted-sperm |
topic |
Bovine Immuno-sexing Proteomics Sex-sorted-sperm |
description |
The identification of distinct proteins present on the membrane of spermatozoa with X and Y chromosomes allows the development of immuno-sexing techniques. The aim of this study, therefore, was to use mass spectrometry to analyze the protein profile of sperm previously categorized using flow cytometry into X or Y-bearing semen pools. Sex-sorted sperm samples (n = 6 X and n = 6 Y) were used. Proteins were extracted and analyzed by mass spectrometry using data independent acquisition (DIA). The data were searched against taxonomy Bos taurus in the Swiss Prot database. In total, 459 protein groups were identified. Of these, eight proteins were in differential abundances between the X- and Y-bearing sperm population. Among the major proteinsdetected, EF-hand domain-containing protein 1, a protein involved in embryonic development, is more abundant in Y-bearing spermatozoa. In addition, proteins FUN14, domain-containing protein 2, NADH dehydrogenase [ubiquinone] iron-sulfur protein 7 mitochondrial, cytochrome C oxidase subunit 2, acetyl -CoA carboxylase type beta were more abundant in X-bearing sperm. In conclusion, there were differences in abundance of proteins between X- and Y-bearing bull spermatozoa. This fact, may contribute to future studies related to sperm physiology and possibility development of immuno-sexing techniques. |
publishDate |
2018 |
dc.date.none.fl_str_mv |
2018-11-01 2019-10-06T15:57:44Z 2019-10-06T15:57:44Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1016/j.anireprosci.2018.09.010 Animal Reproduction Science, v. 198, p. 121-128. 0378-4320 http://hdl.handle.net/11449/188114 10.1016/j.anireprosci.2018.09.010 2-s2.0-85054001326 |
url |
http://dx.doi.org/10.1016/j.anireprosci.2018.09.010 http://hdl.handle.net/11449/188114 |
identifier_str_mv |
Animal Reproduction Science, v. 198, p. 121-128. 0378-4320 10.1016/j.anireprosci.2018.09.010 2-s2.0-85054001326 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Animal Reproduction Science |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
121-128 |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
repositoriounesp@unesp.br |
_version_ |
1810021328045998080 |