Urinary Mutagenicity in Chemical Laboratory Workers Exposed to Solvents
Autor(a) principal: | |
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Data de Publicação: | 2008 |
Outros Autores: | , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1539/joh.L7151 http://hdl.handle.net/11449/7507 |
Resumo: | Solvents represent an important group of environmental pollutants to which people are exposed daily in the workplace. The physico chemical properties of solvents may result in disturbances to cellular structures, including damage to DNA. However, the effects of mixtures of solvents are not well known. Mutations caused by environmental agents are related to cancer development and other degenerative diseases. The work in a research laboratory that uses several types of solvents is equally predisposed to these hazards. In this study, we evaluated the mutagenicity of urine from 29 subjects exposed occupationally to solvents in a chemistry research laboratory and 29 subjects without occupational exposure (controls). Urine samples were collected in polyethylene containers at the end of the work shift. For the concentration and extraction of urine samples the XAD-2 resin was used with acetone as an eluting agent. Several strains of Salmonella typhimurium (TA100, TA98, TA97a, TA1535, YG1024) should be used to assess mutagenic susceptibilities among workers exposed to organic solvents. Different doses of extract (1.5; 3.0; 6.0 and 12.0 m/ equivalents of urine per plate) were tested on S. typhimurium strains TA100 and YG 1024, with and without metabolic activation. The mutagenic activity, measured in Salmonella typhimurium YGI1024 with S9 mix, was significantly greater in urine from workers than from controls (p <= 0.05). These results indicate the relevance of using biomarkers to assess the risk of occupational exposure to organic solvents. |
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Repositório Institucional da UNESP |
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Urinary Mutagenicity in Chemical Laboratory Workers Exposed to SolventsUrine mutagenicityGenotoxic exposureSolventsSalmonellaReversion assaySolvents represent an important group of environmental pollutants to which people are exposed daily in the workplace. The physico chemical properties of solvents may result in disturbances to cellular structures, including damage to DNA. However, the effects of mixtures of solvents are not well known. Mutations caused by environmental agents are related to cancer development and other degenerative diseases. The work in a research laboratory that uses several types of solvents is equally predisposed to these hazards. In this study, we evaluated the mutagenicity of urine from 29 subjects exposed occupationally to solvents in a chemistry research laboratory and 29 subjects without occupational exposure (controls). Urine samples were collected in polyethylene containers at the end of the work shift. For the concentration and extraction of urine samples the XAD-2 resin was used with acetone as an eluting agent. Several strains of Salmonella typhimurium (TA100, TA98, TA97a, TA1535, YG1024) should be used to assess mutagenic susceptibilities among workers exposed to organic solvents. Different doses of extract (1.5; 3.0; 6.0 and 12.0 m/ equivalents of urine per plate) were tested on S. typhimurium strains TA100 and YG 1024, with and without metabolic activation. The mutagenic activity, measured in Salmonella typhimurium YGI1024 with S9 mix, was significantly greater in urine from workers than from controls (p <= 0.05). These results indicate the relevance of using biomarkers to assess the risk of occupational exposure to organic solvents.Univ Estadual Paulista, Fac Pharmaceut Sci Araraquara, Dept Biol Sci, BR-14801902 São Paulo, BrazilUniv Estadual Paulista, Fac Pharmaceut Sci Araraquara, Dept Biol Sci, BR-14801902 São Paulo, BrazilJapan Soc Occupational HealthUniversidade Estadual Paulista (Unesp)Varella, Soraya D. [UNESP]Rampazo, Raquel A. [UNESP]Varanda, Eliana Aparecida [UNESP]2014-05-20T13:24:20Z2014-05-20T13:24:20Z2008-09-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article415-422application/pdfhttp://dx.doi.org/10.1539/joh.L7151Journal of Occupational Health. Tokyo: Japan Soc Occupational Health, v. 50, n. 5, p. 415-422, 2008.1341-9145http://hdl.handle.net/11449/750710.1539/joh.L7151WOS:000259909300006WOS000259909300006.pdf7501930236496670Web of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengJournal of Occupational Health1.2850,491info:eu-repo/semantics/openAccess2024-06-24T13:08:02Zoai:repositorio.unesp.br:11449/7507Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T21:04:21.736087Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Urinary Mutagenicity in Chemical Laboratory Workers Exposed to Solvents |
title |
Urinary Mutagenicity in Chemical Laboratory Workers Exposed to Solvents |
spellingShingle |
Urinary Mutagenicity in Chemical Laboratory Workers Exposed to Solvents Varella, Soraya D. [UNESP] Urine mutagenicity Genotoxic exposure Solvents Salmonella Reversion assay |
title_short |
Urinary Mutagenicity in Chemical Laboratory Workers Exposed to Solvents |
title_full |
Urinary Mutagenicity in Chemical Laboratory Workers Exposed to Solvents |
title_fullStr |
Urinary Mutagenicity in Chemical Laboratory Workers Exposed to Solvents |
title_full_unstemmed |
Urinary Mutagenicity in Chemical Laboratory Workers Exposed to Solvents |
title_sort |
Urinary Mutagenicity in Chemical Laboratory Workers Exposed to Solvents |
author |
Varella, Soraya D. [UNESP] |
author_facet |
Varella, Soraya D. [UNESP] Rampazo, Raquel A. [UNESP] Varanda, Eliana Aparecida [UNESP] |
author_role |
author |
author2 |
Rampazo, Raquel A. [UNESP] Varanda, Eliana Aparecida [UNESP] |
author2_role |
author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Varella, Soraya D. [UNESP] Rampazo, Raquel A. [UNESP] Varanda, Eliana Aparecida [UNESP] |
dc.subject.por.fl_str_mv |
Urine mutagenicity Genotoxic exposure Solvents Salmonella Reversion assay |
topic |
Urine mutagenicity Genotoxic exposure Solvents Salmonella Reversion assay |
description |
Solvents represent an important group of environmental pollutants to which people are exposed daily in the workplace. The physico chemical properties of solvents may result in disturbances to cellular structures, including damage to DNA. However, the effects of mixtures of solvents are not well known. Mutations caused by environmental agents are related to cancer development and other degenerative diseases. The work in a research laboratory that uses several types of solvents is equally predisposed to these hazards. In this study, we evaluated the mutagenicity of urine from 29 subjects exposed occupationally to solvents in a chemistry research laboratory and 29 subjects without occupational exposure (controls). Urine samples were collected in polyethylene containers at the end of the work shift. For the concentration and extraction of urine samples the XAD-2 resin was used with acetone as an eluting agent. Several strains of Salmonella typhimurium (TA100, TA98, TA97a, TA1535, YG1024) should be used to assess mutagenic susceptibilities among workers exposed to organic solvents. Different doses of extract (1.5; 3.0; 6.0 and 12.0 m/ equivalents of urine per plate) were tested on S. typhimurium strains TA100 and YG 1024, with and without metabolic activation. The mutagenic activity, measured in Salmonella typhimurium YGI1024 with S9 mix, was significantly greater in urine from workers than from controls (p <= 0.05). These results indicate the relevance of using biomarkers to assess the risk of occupational exposure to organic solvents. |
publishDate |
2008 |
dc.date.none.fl_str_mv |
2008-09-01 2014-05-20T13:24:20Z 2014-05-20T13:24:20Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1539/joh.L7151 Journal of Occupational Health. Tokyo: Japan Soc Occupational Health, v. 50, n. 5, p. 415-422, 2008. 1341-9145 http://hdl.handle.net/11449/7507 10.1539/joh.L7151 WOS:000259909300006 WOS000259909300006.pdf 7501930236496670 |
url |
http://dx.doi.org/10.1539/joh.L7151 http://hdl.handle.net/11449/7507 |
identifier_str_mv |
Journal of Occupational Health. Tokyo: Japan Soc Occupational Health, v. 50, n. 5, p. 415-422, 2008. 1341-9145 10.1539/joh.L7151 WOS:000259909300006 WOS000259909300006.pdf 7501930236496670 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Journal of Occupational Health 1.285 0,491 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
415-422 application/pdf |
dc.publisher.none.fl_str_mv |
Japan Soc Occupational Health |
publisher.none.fl_str_mv |
Japan Soc Occupational Health |
dc.source.none.fl_str_mv |
Web of Science reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808129280946733056 |