CO2 exposure enhances Fos expression in hypothalamic neurons in rats during the light and dark phases of the diurnal cycle
Autor(a) principal: | |
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Data de Publicação: | 2022 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1007/s00429-022-02562-2 http://hdl.handle.net/11449/237877 |
Resumo: | Orexinergic (OX) neurons in the lateral hypothalamus (LH), perifornical area (PFA) and dorsomedial hypothalamus (DMH) play a role in the hypercapnic ventilatory response, presumably through direct inputs to central pattern generator sites and/or through interactions with other chemosensitive regions. OX neurons can produce and release orexins, excitatory neuropeptides involved in many functions, including physiological responses to changes in CO2/pH. Thus, in the present study, we tested the hypothesis that different nuclei (LH, PFA and DMH) where the orexinergic neurons are located, show distinct activation by CO2 during the light-dark cycle phases. For this purpose, we evaluated the Fos and OXA expression by immunohistochemistry to identify neurons that co-localize Fos + OXA in the LH, LPeF, MPeF and DMH in the light-inactive and dark-active phase in Wistar rats subjected to 3 h of normocapnia or hypercapnia (7% CO2). Quantitative analyses of immunoreactive neurons show that hypercapnia caused an increase in the number of neurons expressing Fos in the LH, LPeF, MPeF and DMH in the light and dark phases. In addition, the number of Fos + OXA neurons increased in the LPeF and DMH independently of the phases of the diurnal cycle; whereas in the MPeF, this increase was observed exclusively in the light phase. Thus, we suggest that OX neurons are selectively activated by hypercapnia throughout the diurnal cycle, reinforcing the differential role of nuclei in the hypothalamus during central chemosensitivity. |
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Repositório Institucional da UNESP |
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2946 |
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CO2 exposure enhances Fos expression in hypothalamic neurons in rats during the light and dark phases of the diurnal cycleCentral chemoreflexHypercapniaHypothalamusOrexinsOrexinergic (OX) neurons in the lateral hypothalamus (LH), perifornical area (PFA) and dorsomedial hypothalamus (DMH) play a role in the hypercapnic ventilatory response, presumably through direct inputs to central pattern generator sites and/or through interactions with other chemosensitive regions. OX neurons can produce and release orexins, excitatory neuropeptides involved in many functions, including physiological responses to changes in CO2/pH. Thus, in the present study, we tested the hypothesis that different nuclei (LH, PFA and DMH) where the orexinergic neurons are located, show distinct activation by CO2 during the light-dark cycle phases. For this purpose, we evaluated the Fos and OXA expression by immunohistochemistry to identify neurons that co-localize Fos + OXA in the LH, LPeF, MPeF and DMH in the light-inactive and dark-active phase in Wistar rats subjected to 3 h of normocapnia or hypercapnia (7% CO2). Quantitative analyses of immunoreactive neurons show that hypercapnia caused an increase in the number of neurons expressing Fos in the LH, LPeF, MPeF and DMH in the light and dark phases. In addition, the number of Fos + OXA neurons increased in the LPeF and DMH independently of the phases of the diurnal cycle; whereas in the MPeF, this increase was observed exclusively in the light phase. Thus, we suggest that OX neurons are selectively activated by hypercapnia throughout the diurnal cycle, reinforcing the differential role of nuclei in the hypothalamus during central chemosensitivity.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Sao Paulo State Univ UNESP, Inst Biosci, Dept Struct & Funct Biol, Botucatu, SP, BrazilSao Paulo State Univ, Fac Agr & Vet Sci, Dept Anim Morphol & Physiol, Jaboticabal, SP, BrazilSao Paulo State Univ UNESP, Inst Biosci, Dept Struct & Funct Biol, Botucatu, SP, BrazilSao Paulo State Univ, Fac Agr & Vet Sci, Dept Anim Morphol & Physiol, Jaboticabal, SP, BrazilFAPESP: 2013/04216-8FAPESP: 2019/17693-5FAPESP: 2016/131366CAPES: 001CAPES: 88887.194785/2018-00SpringerUniversidade Estadual Paulista (UNESP)Silva, Eliandra N. da [UNESP]Horta-Junior, Jose de Anchieta C. [UNESP]Gargaglioni, Luciane H. [UNESP]Dias, Mirela B. [UNESP]2022-11-30T13:47:21Z2022-11-30T13:47:21Z2022-09-15info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article13http://dx.doi.org/10.1007/s00429-022-02562-2Brain Structure & Function. Heidelberg: Springer Heidelberg, 13 p., 2022.1863-2653http://hdl.handle.net/11449/23787710.1007/s00429-022-02562-2WOS:000854692800001Web of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengBrain Structure & Functioninfo:eu-repo/semantics/openAccess2024-06-06T18:41:41Zoai:repositorio.unesp.br:11449/237877Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-06-06T18:41:41Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
CO2 exposure enhances Fos expression in hypothalamic neurons in rats during the light and dark phases of the diurnal cycle |
title |
CO2 exposure enhances Fos expression in hypothalamic neurons in rats during the light and dark phases of the diurnal cycle |
spellingShingle |
CO2 exposure enhances Fos expression in hypothalamic neurons in rats during the light and dark phases of the diurnal cycle Silva, Eliandra N. da [UNESP] Central chemoreflex Hypercapnia Hypothalamus Orexins |
title_short |
CO2 exposure enhances Fos expression in hypothalamic neurons in rats during the light and dark phases of the diurnal cycle |
title_full |
CO2 exposure enhances Fos expression in hypothalamic neurons in rats during the light and dark phases of the diurnal cycle |
title_fullStr |
CO2 exposure enhances Fos expression in hypothalamic neurons in rats during the light and dark phases of the diurnal cycle |
title_full_unstemmed |
CO2 exposure enhances Fos expression in hypothalamic neurons in rats during the light and dark phases of the diurnal cycle |
title_sort |
CO2 exposure enhances Fos expression in hypothalamic neurons in rats during the light and dark phases of the diurnal cycle |
author |
Silva, Eliandra N. da [UNESP] |
author_facet |
Silva, Eliandra N. da [UNESP] Horta-Junior, Jose de Anchieta C. [UNESP] Gargaglioni, Luciane H. [UNESP] Dias, Mirela B. [UNESP] |
author_role |
author |
author2 |
Horta-Junior, Jose de Anchieta C. [UNESP] Gargaglioni, Luciane H. [UNESP] Dias, Mirela B. [UNESP] |
author2_role |
author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (UNESP) |
dc.contributor.author.fl_str_mv |
Silva, Eliandra N. da [UNESP] Horta-Junior, Jose de Anchieta C. [UNESP] Gargaglioni, Luciane H. [UNESP] Dias, Mirela B. [UNESP] |
dc.subject.por.fl_str_mv |
Central chemoreflex Hypercapnia Hypothalamus Orexins |
topic |
Central chemoreflex Hypercapnia Hypothalamus Orexins |
description |
Orexinergic (OX) neurons in the lateral hypothalamus (LH), perifornical area (PFA) and dorsomedial hypothalamus (DMH) play a role in the hypercapnic ventilatory response, presumably through direct inputs to central pattern generator sites and/or through interactions with other chemosensitive regions. OX neurons can produce and release orexins, excitatory neuropeptides involved in many functions, including physiological responses to changes in CO2/pH. Thus, in the present study, we tested the hypothesis that different nuclei (LH, PFA and DMH) where the orexinergic neurons are located, show distinct activation by CO2 during the light-dark cycle phases. For this purpose, we evaluated the Fos and OXA expression by immunohistochemistry to identify neurons that co-localize Fos + OXA in the LH, LPeF, MPeF and DMH in the light-inactive and dark-active phase in Wistar rats subjected to 3 h of normocapnia or hypercapnia (7% CO2). Quantitative analyses of immunoreactive neurons show that hypercapnia caused an increase in the number of neurons expressing Fos in the LH, LPeF, MPeF and DMH in the light and dark phases. In addition, the number of Fos + OXA neurons increased in the LPeF and DMH independently of the phases of the diurnal cycle; whereas in the MPeF, this increase was observed exclusively in the light phase. Thus, we suggest that OX neurons are selectively activated by hypercapnia throughout the diurnal cycle, reinforcing the differential role of nuclei in the hypothalamus during central chemosensitivity. |
publishDate |
2022 |
dc.date.none.fl_str_mv |
2022-11-30T13:47:21Z 2022-11-30T13:47:21Z 2022-09-15 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1007/s00429-022-02562-2 Brain Structure & Function. Heidelberg: Springer Heidelberg, 13 p., 2022. 1863-2653 http://hdl.handle.net/11449/237877 10.1007/s00429-022-02562-2 WOS:000854692800001 |
url |
http://dx.doi.org/10.1007/s00429-022-02562-2 http://hdl.handle.net/11449/237877 |
identifier_str_mv |
Brain Structure & Function. Heidelberg: Springer Heidelberg, 13 p., 2022. 1863-2653 10.1007/s00429-022-02562-2 WOS:000854692800001 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Brain Structure & Function |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
13 |
dc.publisher.none.fl_str_mv |
Springer |
publisher.none.fl_str_mv |
Springer |
dc.source.none.fl_str_mv |
Web of Science reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1803045349650071552 |