Virulence factors and phylogenetic group profile of uterine Escherichia coli in early postpartum of high-producing dairy cows
Autor(a) principal: | |
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Data de Publicação: | 2019 |
Outros Autores: | , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
DOI: | 10.1071/AN17729 |
Texto Completo: | http://dx.doi.org/10.1071/AN17729 http://hdl.handle.net/11449/188702 |
Resumo: | Escherichia coli is the most common contaminant of the bovine uterus in the first few weeks postpartum, and one of the most important pathogens involved in uterine infections. This bacterium is characterised by diverse virulence factors (VF) however, the profile of E. coli VF in physiologic postpartum uterine contamination is not well established. Therefore, the present study aimed to investigate the prevalence of intrauterine bacteria, a set of virulence factors and phylogroups of E. coli isolates, leukogram and uterine cytology in 75 Holstein cows at 24 h (Sampling time 1) and 14 days (Sampling time 2) postpartum. Escherichia coli, α-haemolytic Streptococcus, Trueperella pyogenes, and Pasteurella multocida were the most common microorganisms isolated in pure culture, whereas E. coli and Clostridium perfringens Type A, E. coli and α-haemolytic Streptococcus, and E. coli and Proteus mirabilis were the most frequent microorganisms in mixed colonies at both studied sampling times. In the 59 E. coli isolates after 24 h (n = 35) and 14 days (n = 24) postpartum, the genes detected for VF were fimH, iucD/aer, kps, hlyA, usp, vt1 and vt2. Most E. coli strains detected in both moments of study belonged to Commensal phylogenetic groups A and B1, whereas Pathogenic groups D and B2 were identified at 24 h and 14 days postpartum respectively. All cows presented suitable immune response against the presence of bacteria in uterine lumen, observed by leukocytosis, neutrophilia, lymphocytosis and monocytosis at leukogram and a high number of polymorphonuclear leukocytes in uterine cytology, in both studied moments. Thus, a complex diversity of aerobic and anaerobic bacteria is involved in uterine contamination in the early postpartum of cows, besides the predominance of E. coli. Moreover, the genes fimH, iucD/aer, hlyA, kps, usp, vt1 and vt2 play a key role in the virulence of E. coli in this period. |
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Virulence factors and phylogenetic group profile of uterine Escherichia coli in early postpartum of high-producing dairy cowsanimal reproductionbacteriologygenetic variationmicrobial diversityEscherichia coli is the most common contaminant of the bovine uterus in the first few weeks postpartum, and one of the most important pathogens involved in uterine infections. This bacterium is characterised by diverse virulence factors (VF) however, the profile of E. coli VF in physiologic postpartum uterine contamination is not well established. Therefore, the present study aimed to investigate the prevalence of intrauterine bacteria, a set of virulence factors and phylogroups of E. coli isolates, leukogram and uterine cytology in 75 Holstein cows at 24 h (Sampling time 1) and 14 days (Sampling time 2) postpartum. Escherichia coli, α-haemolytic Streptococcus, Trueperella pyogenes, and Pasteurella multocida were the most common microorganisms isolated in pure culture, whereas E. coli and Clostridium perfringens Type A, E. coli and α-haemolytic Streptococcus, and E. coli and Proteus mirabilis were the most frequent microorganisms in mixed colonies at both studied sampling times. In the 59 E. coli isolates after 24 h (n = 35) and 14 days (n = 24) postpartum, the genes detected for VF were fimH, iucD/aer, kps, hlyA, usp, vt1 and vt2. Most E. coli strains detected in both moments of study belonged to Commensal phylogenetic groups A and B1, whereas Pathogenic groups D and B2 were identified at 24 h and 14 days postpartum respectively. All cows presented suitable immune response against the presence of bacteria in uterine lumen, observed by leukocytosis, neutrophilia, lymphocytosis and monocytosis at leukogram and a high number of polymorphonuclear leukocytes in uterine cytology, in both studied moments. Thus, a complex diversity of aerobic and anaerobic bacteria is involved in uterine contamination in the early postpartum of cows, besides the predominance of E. coli. Moreover, the genes fimH, iucD/aer, hlyA, kps, usp, vt1 and vt2 play a key role in the virulence of E. coli in this period.Department of Animal Reproduction and Veterinary Radiology School of Veterinary Medicine and Animal Science (FMVZ) São Paulo State University (UNESP), Prof. Doutor Walter Mauricio Correa StreetDepartment of Genetics Evolution and Bioagents Institute of Biology University of Campinas, 255 Monteiro Lobato Street, Postal Address 6109Department of Veterinary Clinics School of Veterinary Medicine and Animal Science (FMVZ) São Paulo State University (UNESP), Prof. Doutor Walter Mauricio Correa StreetDepartment of Veterinary Hygiene and Public Health School of Veterinary Medicine and Animal Sciences (FMVZ) São Paulo State University (UNESP) Prof. Doutor Walter Mauricio Correa Street Rubião Junior Postal Address 560Department of Animal Reproduction and Veterinary Radiology School of Veterinary Medicine and Animal Science (FMVZ) São Paulo State University (UNESP), Prof. Doutor Walter Mauricio Correa StreetDepartment of Veterinary Clinics School of Veterinary Medicine and Animal Science (FMVZ) São Paulo State University (UNESP), Prof. Doutor Walter Mauricio Correa StreetDepartment of Veterinary Hygiene and Public Health School of Veterinary Medicine and Animal Sciences (FMVZ) São Paulo State University (UNESP) Prof. Doutor Walter Mauricio Correa Street Rubião Junior Postal Address 560Universidade Estadual Paulista (Unesp)Universidade Estadual de Campinas (UNICAMP)De Cássia Bicudo, Luana [UNESP]Oba, Eunice [UNESP]Bicudo, Sony Dimas [UNESP]Da Silva Leite, DomingosSiqueira, Amanda KellerDe Souza Monobe, Marina Mitie [UNESP]Nogueira, MeghiDe Figueiredo Pantoja, José Carlos [UNESP]Listoni, Fernando José Paganini [UNESP]Ribeiro, Márcio Garcia [UNESP]2019-10-06T16:16:34Z2019-10-06T16:16:34Z2019-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article1898-1905http://dx.doi.org/10.1071/AN17729Animal Production Science, v. 59, n. 10, p. 1898-1905, 2019.1836-57871836-0939http://hdl.handle.net/11449/18870210.1071/AN177292-s2.0-85061102660Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengAnimal Production Scienceinfo:eu-repo/semantics/openAccess2024-09-09T14:01:31Zoai:repositorio.unesp.br:11449/188702Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestrepositoriounesp@unesp.bropendoar:29462024-09-09T14:01:31Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Virulence factors and phylogenetic group profile of uterine Escherichia coli in early postpartum of high-producing dairy cows |
title |
Virulence factors and phylogenetic group profile of uterine Escherichia coli in early postpartum of high-producing dairy cows |
spellingShingle |
Virulence factors and phylogenetic group profile of uterine Escherichia coli in early postpartum of high-producing dairy cows Virulence factors and phylogenetic group profile of uterine Escherichia coli in early postpartum of high-producing dairy cows De Cássia Bicudo, Luana [UNESP] animal reproduction bacteriology genetic variation microbial diversity De Cássia Bicudo, Luana [UNESP] animal reproduction bacteriology genetic variation microbial diversity |
title_short |
Virulence factors and phylogenetic group profile of uterine Escherichia coli in early postpartum of high-producing dairy cows |
title_full |
Virulence factors and phylogenetic group profile of uterine Escherichia coli in early postpartum of high-producing dairy cows |
title_fullStr |
Virulence factors and phylogenetic group profile of uterine Escherichia coli in early postpartum of high-producing dairy cows Virulence factors and phylogenetic group profile of uterine Escherichia coli in early postpartum of high-producing dairy cows |
title_full_unstemmed |
Virulence factors and phylogenetic group profile of uterine Escherichia coli in early postpartum of high-producing dairy cows Virulence factors and phylogenetic group profile of uterine Escherichia coli in early postpartum of high-producing dairy cows |
title_sort |
Virulence factors and phylogenetic group profile of uterine Escherichia coli in early postpartum of high-producing dairy cows |
author |
De Cássia Bicudo, Luana [UNESP] |
author_facet |
De Cássia Bicudo, Luana [UNESP] De Cássia Bicudo, Luana [UNESP] Oba, Eunice [UNESP] Bicudo, Sony Dimas [UNESP] Da Silva Leite, Domingos Siqueira, Amanda Keller De Souza Monobe, Marina Mitie [UNESP] Nogueira, Meghi De Figueiredo Pantoja, José Carlos [UNESP] Listoni, Fernando José Paganini [UNESP] Ribeiro, Márcio Garcia [UNESP] Oba, Eunice [UNESP] Bicudo, Sony Dimas [UNESP] Da Silva Leite, Domingos Siqueira, Amanda Keller De Souza Monobe, Marina Mitie [UNESP] Nogueira, Meghi De Figueiredo Pantoja, José Carlos [UNESP] Listoni, Fernando José Paganini [UNESP] Ribeiro, Márcio Garcia [UNESP] |
author_role |
author |
author2 |
Oba, Eunice [UNESP] Bicudo, Sony Dimas [UNESP] Da Silva Leite, Domingos Siqueira, Amanda Keller De Souza Monobe, Marina Mitie [UNESP] Nogueira, Meghi De Figueiredo Pantoja, José Carlos [UNESP] Listoni, Fernando José Paganini [UNESP] Ribeiro, Márcio Garcia [UNESP] |
author2_role |
author author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) Universidade Estadual de Campinas (UNICAMP) |
dc.contributor.author.fl_str_mv |
De Cássia Bicudo, Luana [UNESP] Oba, Eunice [UNESP] Bicudo, Sony Dimas [UNESP] Da Silva Leite, Domingos Siqueira, Amanda Keller De Souza Monobe, Marina Mitie [UNESP] Nogueira, Meghi De Figueiredo Pantoja, José Carlos [UNESP] Listoni, Fernando José Paganini [UNESP] Ribeiro, Márcio Garcia [UNESP] |
dc.subject.por.fl_str_mv |
animal reproduction bacteriology genetic variation microbial diversity |
topic |
animal reproduction bacteriology genetic variation microbial diversity |
description |
Escherichia coli is the most common contaminant of the bovine uterus in the first few weeks postpartum, and one of the most important pathogens involved in uterine infections. This bacterium is characterised by diverse virulence factors (VF) however, the profile of E. coli VF in physiologic postpartum uterine contamination is not well established. Therefore, the present study aimed to investigate the prevalence of intrauterine bacteria, a set of virulence factors and phylogroups of E. coli isolates, leukogram and uterine cytology in 75 Holstein cows at 24 h (Sampling time 1) and 14 days (Sampling time 2) postpartum. Escherichia coli, α-haemolytic Streptococcus, Trueperella pyogenes, and Pasteurella multocida were the most common microorganisms isolated in pure culture, whereas E. coli and Clostridium perfringens Type A, E. coli and α-haemolytic Streptococcus, and E. coli and Proteus mirabilis were the most frequent microorganisms in mixed colonies at both studied sampling times. In the 59 E. coli isolates after 24 h (n = 35) and 14 days (n = 24) postpartum, the genes detected for VF were fimH, iucD/aer, kps, hlyA, usp, vt1 and vt2. Most E. coli strains detected in both moments of study belonged to Commensal phylogenetic groups A and B1, whereas Pathogenic groups D and B2 were identified at 24 h and 14 days postpartum respectively. All cows presented suitable immune response against the presence of bacteria in uterine lumen, observed by leukocytosis, neutrophilia, lymphocytosis and monocytosis at leukogram and a high number of polymorphonuclear leukocytes in uterine cytology, in both studied moments. Thus, a complex diversity of aerobic and anaerobic bacteria is involved in uterine contamination in the early postpartum of cows, besides the predominance of E. coli. Moreover, the genes fimH, iucD/aer, hlyA, kps, usp, vt1 and vt2 play a key role in the virulence of E. coli in this period. |
publishDate |
2019 |
dc.date.none.fl_str_mv |
2019-10-06T16:16:34Z 2019-10-06T16:16:34Z 2019-01-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1071/AN17729 Animal Production Science, v. 59, n. 10, p. 1898-1905, 2019. 1836-5787 1836-0939 http://hdl.handle.net/11449/188702 10.1071/AN17729 2-s2.0-85061102660 |
url |
http://dx.doi.org/10.1071/AN17729 http://hdl.handle.net/11449/188702 |
identifier_str_mv |
Animal Production Science, v. 59, n. 10, p. 1898-1905, 2019. 1836-5787 1836-0939 10.1071/AN17729 2-s2.0-85061102660 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Animal Production Science |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
1898-1905 |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
repositoriounesp@unesp.br |
_version_ |
1822183850071031808 |
dc.identifier.doi.none.fl_str_mv |
10.1071/AN17729 |