Virulence factors and phylogenetic group profile of uterine Escherichia coli in early postpartum of high-producing dairy cows

Detalhes bibliográficos
Autor(a) principal: De Cássia Bicudo, Luana [UNESP]
Data de Publicação: 2019
Outros Autores: Oba, Eunice [UNESP], Bicudo, Sony Dimas [UNESP], Da Silva Leite, Domingos, Siqueira, Amanda Keller, De Souza Monobe, Marina Mitie [UNESP], Nogueira, Meghi, De Figueiredo Pantoja, José Carlos [UNESP], Listoni, Fernando José Paganini [UNESP], Ribeiro, Márcio Garcia [UNESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
DOI: 10.1071/AN17729
Texto Completo: http://dx.doi.org/10.1071/AN17729
http://hdl.handle.net/11449/188702
Resumo: Escherichia coli is the most common contaminant of the bovine uterus in the first few weeks postpartum, and one of the most important pathogens involved in uterine infections. This bacterium is characterised by diverse virulence factors (VF) however, the profile of E. coli VF in physiologic postpartum uterine contamination is not well established. Therefore, the present study aimed to investigate the prevalence of intrauterine bacteria, a set of virulence factors and phylogroups of E. coli isolates, leukogram and uterine cytology in 75 Holstein cows at 24 h (Sampling time 1) and 14 days (Sampling time 2) postpartum. Escherichia coli, α-haemolytic Streptococcus, Trueperella pyogenes, and Pasteurella multocida were the most common microorganisms isolated in pure culture, whereas E. coli and Clostridium perfringens Type A, E. coli and α-haemolytic Streptococcus, and E. coli and Proteus mirabilis were the most frequent microorganisms in mixed colonies at both studied sampling times. In the 59 E. coli isolates after 24 h (n = 35) and 14 days (n = 24) postpartum, the genes detected for VF were fimH, iucD/aer, kps, hlyA, usp, vt1 and vt2. Most E. coli strains detected in both moments of study belonged to Commensal phylogenetic groups A and B1, whereas Pathogenic groups D and B2 were identified at 24 h and 14 days postpartum respectively. All cows presented suitable immune response against the presence of bacteria in uterine lumen, observed by leukocytosis, neutrophilia, lymphocytosis and monocytosis at leukogram and a high number of polymorphonuclear leukocytes in uterine cytology, in both studied moments. Thus, a complex diversity of aerobic and anaerobic bacteria is involved in uterine contamination in the early postpartum of cows, besides the predominance of E. coli. Moreover, the genes fimH, iucD/aer, hlyA, kps, usp, vt1 and vt2 play a key role in the virulence of E. coli in this period.
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spelling Virulence factors and phylogenetic group profile of uterine Escherichia coli in early postpartum of high-producing dairy cowsanimal reproductionbacteriologygenetic variationmicrobial diversityEscherichia coli is the most common contaminant of the bovine uterus in the first few weeks postpartum, and one of the most important pathogens involved in uterine infections. This bacterium is characterised by diverse virulence factors (VF) however, the profile of E. coli VF in physiologic postpartum uterine contamination is not well established. Therefore, the present study aimed to investigate the prevalence of intrauterine bacteria, a set of virulence factors and phylogroups of E. coli isolates, leukogram and uterine cytology in 75 Holstein cows at 24 h (Sampling time 1) and 14 days (Sampling time 2) postpartum. Escherichia coli, α-haemolytic Streptococcus, Trueperella pyogenes, and Pasteurella multocida were the most common microorganisms isolated in pure culture, whereas E. coli and Clostridium perfringens Type A, E. coli and α-haemolytic Streptococcus, and E. coli and Proteus mirabilis were the most frequent microorganisms in mixed colonies at both studied sampling times. In the 59 E. coli isolates after 24 h (n = 35) and 14 days (n = 24) postpartum, the genes detected for VF were fimH, iucD/aer, kps, hlyA, usp, vt1 and vt2. Most E. coli strains detected in both moments of study belonged to Commensal phylogenetic groups A and B1, whereas Pathogenic groups D and B2 were identified at 24 h and 14 days postpartum respectively. All cows presented suitable immune response against the presence of bacteria in uterine lumen, observed by leukocytosis, neutrophilia, lymphocytosis and monocytosis at leukogram and a high number of polymorphonuclear leukocytes in uterine cytology, in both studied moments. Thus, a complex diversity of aerobic and anaerobic bacteria is involved in uterine contamination in the early postpartum of cows, besides the predominance of E. coli. Moreover, the genes fimH, iucD/aer, hlyA, kps, usp, vt1 and vt2 play a key role in the virulence of E. coli in this period.Department of Animal Reproduction and Veterinary Radiology School of Veterinary Medicine and Animal Science (FMVZ) São Paulo State University (UNESP), Prof. Doutor Walter Mauricio Correa StreetDepartment of Genetics Evolution and Bioagents Institute of Biology University of Campinas, 255 Monteiro Lobato Street, Postal Address 6109Department of Veterinary Clinics School of Veterinary Medicine and Animal Science (FMVZ) São Paulo State University (UNESP), Prof. Doutor Walter Mauricio Correa StreetDepartment of Veterinary Hygiene and Public Health School of Veterinary Medicine and Animal Sciences (FMVZ) São Paulo State University (UNESP) Prof. Doutor Walter Mauricio Correa Street Rubião Junior Postal Address 560Department of Animal Reproduction and Veterinary Radiology School of Veterinary Medicine and Animal Science (FMVZ) São Paulo State University (UNESP), Prof. Doutor Walter Mauricio Correa StreetDepartment of Veterinary Clinics School of Veterinary Medicine and Animal Science (FMVZ) São Paulo State University (UNESP), Prof. Doutor Walter Mauricio Correa StreetDepartment of Veterinary Hygiene and Public Health School of Veterinary Medicine and Animal Sciences (FMVZ) São Paulo State University (UNESP) Prof. Doutor Walter Mauricio Correa Street Rubião Junior Postal Address 560Universidade Estadual Paulista (Unesp)Universidade Estadual de Campinas (UNICAMP)De Cássia Bicudo, Luana [UNESP]Oba, Eunice [UNESP]Bicudo, Sony Dimas [UNESP]Da Silva Leite, DomingosSiqueira, Amanda KellerDe Souza Monobe, Marina Mitie [UNESP]Nogueira, MeghiDe Figueiredo Pantoja, José Carlos [UNESP]Listoni, Fernando José Paganini [UNESP]Ribeiro, Márcio Garcia [UNESP]2019-10-06T16:16:34Z2019-10-06T16:16:34Z2019-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article1898-1905http://dx.doi.org/10.1071/AN17729Animal Production Science, v. 59, n. 10, p. 1898-1905, 2019.1836-57871836-0939http://hdl.handle.net/11449/18870210.1071/AN177292-s2.0-85061102660Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengAnimal Production Scienceinfo:eu-repo/semantics/openAccess2024-09-09T14:01:31Zoai:repositorio.unesp.br:11449/188702Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestrepositoriounesp@unesp.bropendoar:29462024-09-09T14:01:31Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Virulence factors and phylogenetic group profile of uterine Escherichia coli in early postpartum of high-producing dairy cows
title Virulence factors and phylogenetic group profile of uterine Escherichia coli in early postpartum of high-producing dairy cows
spellingShingle Virulence factors and phylogenetic group profile of uterine Escherichia coli in early postpartum of high-producing dairy cows
Virulence factors and phylogenetic group profile of uterine Escherichia coli in early postpartum of high-producing dairy cows
De Cássia Bicudo, Luana [UNESP]
animal reproduction
bacteriology
genetic variation
microbial diversity
De Cássia Bicudo, Luana [UNESP]
animal reproduction
bacteriology
genetic variation
microbial diversity
title_short Virulence factors and phylogenetic group profile of uterine Escherichia coli in early postpartum of high-producing dairy cows
title_full Virulence factors and phylogenetic group profile of uterine Escherichia coli in early postpartum of high-producing dairy cows
title_fullStr Virulence factors and phylogenetic group profile of uterine Escherichia coli in early postpartum of high-producing dairy cows
Virulence factors and phylogenetic group profile of uterine Escherichia coli in early postpartum of high-producing dairy cows
title_full_unstemmed Virulence factors and phylogenetic group profile of uterine Escherichia coli in early postpartum of high-producing dairy cows
Virulence factors and phylogenetic group profile of uterine Escherichia coli in early postpartum of high-producing dairy cows
title_sort Virulence factors and phylogenetic group profile of uterine Escherichia coli in early postpartum of high-producing dairy cows
author De Cássia Bicudo, Luana [UNESP]
author_facet De Cássia Bicudo, Luana [UNESP]
De Cássia Bicudo, Luana [UNESP]
Oba, Eunice [UNESP]
Bicudo, Sony Dimas [UNESP]
Da Silva Leite, Domingos
Siqueira, Amanda Keller
De Souza Monobe, Marina Mitie [UNESP]
Nogueira, Meghi
De Figueiredo Pantoja, José Carlos [UNESP]
Listoni, Fernando José Paganini [UNESP]
Ribeiro, Márcio Garcia [UNESP]
Oba, Eunice [UNESP]
Bicudo, Sony Dimas [UNESP]
Da Silva Leite, Domingos
Siqueira, Amanda Keller
De Souza Monobe, Marina Mitie [UNESP]
Nogueira, Meghi
De Figueiredo Pantoja, José Carlos [UNESP]
Listoni, Fernando José Paganini [UNESP]
Ribeiro, Márcio Garcia [UNESP]
author_role author
author2 Oba, Eunice [UNESP]
Bicudo, Sony Dimas [UNESP]
Da Silva Leite, Domingos
Siqueira, Amanda Keller
De Souza Monobe, Marina Mitie [UNESP]
Nogueira, Meghi
De Figueiredo Pantoja, José Carlos [UNESP]
Listoni, Fernando José Paganini [UNESP]
Ribeiro, Márcio Garcia [UNESP]
author2_role author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade Estadual Paulista (Unesp)
Universidade Estadual de Campinas (UNICAMP)
dc.contributor.author.fl_str_mv De Cássia Bicudo, Luana [UNESP]
Oba, Eunice [UNESP]
Bicudo, Sony Dimas [UNESP]
Da Silva Leite, Domingos
Siqueira, Amanda Keller
De Souza Monobe, Marina Mitie [UNESP]
Nogueira, Meghi
De Figueiredo Pantoja, José Carlos [UNESP]
Listoni, Fernando José Paganini [UNESP]
Ribeiro, Márcio Garcia [UNESP]
dc.subject.por.fl_str_mv animal reproduction
bacteriology
genetic variation
microbial diversity
topic animal reproduction
bacteriology
genetic variation
microbial diversity
description Escherichia coli is the most common contaminant of the bovine uterus in the first few weeks postpartum, and one of the most important pathogens involved in uterine infections. This bacterium is characterised by diverse virulence factors (VF) however, the profile of E. coli VF in physiologic postpartum uterine contamination is not well established. Therefore, the present study aimed to investigate the prevalence of intrauterine bacteria, a set of virulence factors and phylogroups of E. coli isolates, leukogram and uterine cytology in 75 Holstein cows at 24 h (Sampling time 1) and 14 days (Sampling time 2) postpartum. Escherichia coli, α-haemolytic Streptococcus, Trueperella pyogenes, and Pasteurella multocida were the most common microorganisms isolated in pure culture, whereas E. coli and Clostridium perfringens Type A, E. coli and α-haemolytic Streptococcus, and E. coli and Proteus mirabilis were the most frequent microorganisms in mixed colonies at both studied sampling times. In the 59 E. coli isolates after 24 h (n = 35) and 14 days (n = 24) postpartum, the genes detected for VF were fimH, iucD/aer, kps, hlyA, usp, vt1 and vt2. Most E. coli strains detected in both moments of study belonged to Commensal phylogenetic groups A and B1, whereas Pathogenic groups D and B2 were identified at 24 h and 14 days postpartum respectively. All cows presented suitable immune response against the presence of bacteria in uterine lumen, observed by leukocytosis, neutrophilia, lymphocytosis and monocytosis at leukogram and a high number of polymorphonuclear leukocytes in uterine cytology, in both studied moments. Thus, a complex diversity of aerobic and anaerobic bacteria is involved in uterine contamination in the early postpartum of cows, besides the predominance of E. coli. Moreover, the genes fimH, iucD/aer, hlyA, kps, usp, vt1 and vt2 play a key role in the virulence of E. coli in this period.
publishDate 2019
dc.date.none.fl_str_mv 2019-10-06T16:16:34Z
2019-10-06T16:16:34Z
2019-01-01
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1071/AN17729
Animal Production Science, v. 59, n. 10, p. 1898-1905, 2019.
1836-5787
1836-0939
http://hdl.handle.net/11449/188702
10.1071/AN17729
2-s2.0-85061102660
url http://dx.doi.org/10.1071/AN17729
http://hdl.handle.net/11449/188702
identifier_str_mv Animal Production Science, v. 59, n. 10, p. 1898-1905, 2019.
1836-5787
1836-0939
10.1071/AN17729
2-s2.0-85061102660
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Animal Production Science
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 1898-1905
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv repositoriounesp@unesp.br
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dc.identifier.doi.none.fl_str_mv 10.1071/AN17729