Photodynamic therapy-mediated hypericin-loaded nanostructured lipid carriers against vulvovaginal candidiasis
Autor(a) principal: | |
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Data de Publicação: | 2022 |
Outros Autores: | , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1016/j.mycmed.2022.101296 http://hdl.handle.net/11449/241918 |
Resumo: | Introduction and Aim: The indiscriminate use and adverse effects of the main conventional antifungal agents compromise the effectiveness of treating vulvovaginal candidiasis (VVC), mainly caused by the species Candida albicans. This study evaluated the effectiveness of photodynamic therapy (PDT) and the in vitro and in vivo anti-candida potential of the hypericin (HYP)-loaded nanostructured lipid carriers (NLC). Materials and Methods: Empty NLC and NLC-HYP were characterized by the dynamic light scattering technique and transmission electron microscopy to evaluate the average particle size distribution and its morphologies. The in vitro inhibition photodynamic effect of the systems was tested to reduce the planktonic viability of C. albicans. The therapeutic assay photodynamic of the systems was performed to treat VVC in mice. Results: Empty NLC and NLC-HYP presented values of average hydrodynamic diameter, polydispersity index, and ζ-potential from 136 to 133 nm, 0.16 to 0.22, and -18 to -30 mV, respectively, on day 30. Microscopically, the systems showed spherical morphologies and nanoscale particles. Furthermore, in the in vitro inhibition assay, the treatment of PDT with NLC-HYP (NLC-HYP+) showed a significant reduction of the C. albicans planktonic viability compared to YNB negative control after 5 min of LED light irradiation. In the in vivo therapeutic assay, the antifungal group (vaginal antifungal cream) and NLC-HYP+ evaluated in the dark and by PDT, respectively, had a significant log10 reduction in fungal burden compared to the infected group on day 8 of the VVC treatment. Conclusion: Due to the in vitro and in vivo anti-candida potential, PDT-mediated systems can be an effective strategy in VVC therapy. |
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Photodynamic therapy-mediated hypericin-loaded nanostructured lipid carriers against vulvovaginal candidiasisAntifungal activityCandida albicansHypericinNanostructured lipid carriersPhotodynamic therapyIntroduction and Aim: The indiscriminate use and adverse effects of the main conventional antifungal agents compromise the effectiveness of treating vulvovaginal candidiasis (VVC), mainly caused by the species Candida albicans. This study evaluated the effectiveness of photodynamic therapy (PDT) and the in vitro and in vivo anti-candida potential of the hypericin (HYP)-loaded nanostructured lipid carriers (NLC). Materials and Methods: Empty NLC and NLC-HYP were characterized by the dynamic light scattering technique and transmission electron microscopy to evaluate the average particle size distribution and its morphologies. The in vitro inhibition photodynamic effect of the systems was tested to reduce the planktonic viability of C. albicans. The therapeutic assay photodynamic of the systems was performed to treat VVC in mice. Results: Empty NLC and NLC-HYP presented values of average hydrodynamic diameter, polydispersity index, and ζ-potential from 136 to 133 nm, 0.16 to 0.22, and -18 to -30 mV, respectively, on day 30. Microscopically, the systems showed spherical morphologies and nanoscale particles. Furthermore, in the in vitro inhibition assay, the treatment of PDT with NLC-HYP (NLC-HYP+) showed a significant reduction of the C. albicans planktonic viability compared to YNB negative control after 5 min of LED light irradiation. In the in vivo therapeutic assay, the antifungal group (vaginal antifungal cream) and NLC-HYP+ evaluated in the dark and by PDT, respectively, had a significant log10 reduction in fungal burden compared to the infected group on day 8 of the VVC treatment. Conclusion: Due to the in vitro and in vivo anti-candida potential, PDT-mediated systems can be an effective strategy in VVC therapy.School of Pharmaceutical Sciences São Paulo State University (UNESP), São PauloGraduation Program in Pharmaceutical Sciences State University of Paraíba, ParaíbaBiomedical Sciences Research Institute University of Ulster, Northern IrelandSchool of Pharmaceutical Sciences São Paulo State University (UNESP), São PauloUniversidade Estadual Paulista (UNESP)State University of ParaíbaUniversity of UlsterSato, Mariana Rillo [UNESP]Oshiro-Junior, João AugustoRodero, Camila Fernanda [UNESP]Boni, Fernanda Isadora [UNESP]Araújo, Victor Hugo Sousa [UNESP]Bauab, Taís Maria [UNESP]Nicholas, DeanCallan, John F.Chorilli, Marlus [UNESP]2023-03-02T04:20:34Z2023-03-02T04:20:34Z2022-11-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.1016/j.mycmed.2022.101296Journal of Medical Mycology, v. 32, n. 4, 2022.1773-04491156-5233http://hdl.handle.net/11449/24191810.1016/j.mycmed.2022.1012962-s2.0-85131414278Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengJournal of Medical Mycologyinfo:eu-repo/semantics/openAccess2024-06-24T13:45:50Zoai:repositorio.unesp.br:11449/241918Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T19:05:15.988586Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Photodynamic therapy-mediated hypericin-loaded nanostructured lipid carriers against vulvovaginal candidiasis |
title |
Photodynamic therapy-mediated hypericin-loaded nanostructured lipid carriers against vulvovaginal candidiasis |
spellingShingle |
Photodynamic therapy-mediated hypericin-loaded nanostructured lipid carriers against vulvovaginal candidiasis Sato, Mariana Rillo [UNESP] Antifungal activity Candida albicans Hypericin Nanostructured lipid carriers Photodynamic therapy |
title_short |
Photodynamic therapy-mediated hypericin-loaded nanostructured lipid carriers against vulvovaginal candidiasis |
title_full |
Photodynamic therapy-mediated hypericin-loaded nanostructured lipid carriers against vulvovaginal candidiasis |
title_fullStr |
Photodynamic therapy-mediated hypericin-loaded nanostructured lipid carriers against vulvovaginal candidiasis |
title_full_unstemmed |
Photodynamic therapy-mediated hypericin-loaded nanostructured lipid carriers against vulvovaginal candidiasis |
title_sort |
Photodynamic therapy-mediated hypericin-loaded nanostructured lipid carriers against vulvovaginal candidiasis |
author |
Sato, Mariana Rillo [UNESP] |
author_facet |
Sato, Mariana Rillo [UNESP] Oshiro-Junior, João Augusto Rodero, Camila Fernanda [UNESP] Boni, Fernanda Isadora [UNESP] Araújo, Victor Hugo Sousa [UNESP] Bauab, Taís Maria [UNESP] Nicholas, Dean Callan, John F. Chorilli, Marlus [UNESP] |
author_role |
author |
author2 |
Oshiro-Junior, João Augusto Rodero, Camila Fernanda [UNESP] Boni, Fernanda Isadora [UNESP] Araújo, Victor Hugo Sousa [UNESP] Bauab, Taís Maria [UNESP] Nicholas, Dean Callan, John F. Chorilli, Marlus [UNESP] |
author2_role |
author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (UNESP) State University of Paraíba University of Ulster |
dc.contributor.author.fl_str_mv |
Sato, Mariana Rillo [UNESP] Oshiro-Junior, João Augusto Rodero, Camila Fernanda [UNESP] Boni, Fernanda Isadora [UNESP] Araújo, Victor Hugo Sousa [UNESP] Bauab, Taís Maria [UNESP] Nicholas, Dean Callan, John F. Chorilli, Marlus [UNESP] |
dc.subject.por.fl_str_mv |
Antifungal activity Candida albicans Hypericin Nanostructured lipid carriers Photodynamic therapy |
topic |
Antifungal activity Candida albicans Hypericin Nanostructured lipid carriers Photodynamic therapy |
description |
Introduction and Aim: The indiscriminate use and adverse effects of the main conventional antifungal agents compromise the effectiveness of treating vulvovaginal candidiasis (VVC), mainly caused by the species Candida albicans. This study evaluated the effectiveness of photodynamic therapy (PDT) and the in vitro and in vivo anti-candida potential of the hypericin (HYP)-loaded nanostructured lipid carriers (NLC). Materials and Methods: Empty NLC and NLC-HYP were characterized by the dynamic light scattering technique and transmission electron microscopy to evaluate the average particle size distribution and its morphologies. The in vitro inhibition photodynamic effect of the systems was tested to reduce the planktonic viability of C. albicans. The therapeutic assay photodynamic of the systems was performed to treat VVC in mice. Results: Empty NLC and NLC-HYP presented values of average hydrodynamic diameter, polydispersity index, and ζ-potential from 136 to 133 nm, 0.16 to 0.22, and -18 to -30 mV, respectively, on day 30. Microscopically, the systems showed spherical morphologies and nanoscale particles. Furthermore, in the in vitro inhibition assay, the treatment of PDT with NLC-HYP (NLC-HYP+) showed a significant reduction of the C. albicans planktonic viability compared to YNB negative control after 5 min of LED light irradiation. In the in vivo therapeutic assay, the antifungal group (vaginal antifungal cream) and NLC-HYP+ evaluated in the dark and by PDT, respectively, had a significant log10 reduction in fungal burden compared to the infected group on day 8 of the VVC treatment. Conclusion: Due to the in vitro and in vivo anti-candida potential, PDT-mediated systems can be an effective strategy in VVC therapy. |
publishDate |
2022 |
dc.date.none.fl_str_mv |
2022-11-01 2023-03-02T04:20:34Z 2023-03-02T04:20:34Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1016/j.mycmed.2022.101296 Journal of Medical Mycology, v. 32, n. 4, 2022. 1773-0449 1156-5233 http://hdl.handle.net/11449/241918 10.1016/j.mycmed.2022.101296 2-s2.0-85131414278 |
url |
http://dx.doi.org/10.1016/j.mycmed.2022.101296 http://hdl.handle.net/11449/241918 |
identifier_str_mv |
Journal of Medical Mycology, v. 32, n. 4, 2022. 1773-0449 1156-5233 10.1016/j.mycmed.2022.101296 2-s2.0-85131414278 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Journal of Medical Mycology |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
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1808129017826508800 |