High p16INK4a immunoexpression is not HPV dependent in oral leukoplakia

Detalhes bibliográficos
Autor(a) principal: Tomo, Saygo [UNESP]
Data de Publicação: 2020
Outros Autores: Biss, Stephanye Pinto [UNESP], Crivelini, Marcelo Macedo [UNESP], de Oliveira, Sandra Helena Penha [UNESP], Biasoli, Éder Ricardo [UNESP], Tjioe, Kellen Cristine [UNESP], Bernabé, Daniel Galera [UNESP], Villa, Luisa Lina, Miyahara, Glauco Issamu [UNESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1016/j.archoralbio.2020.104738
http://hdl.handle.net/11449/200398
Resumo: Objective: To evaluate the prevalence of HPV DNA detection in fresh tissue from oral leukoplakia by Linear Array assay, and its correlation with p16INK4a immunoexpression in the northwest region of the São Paulo state, Brazil. Patients and methods: Fifty patients diagnosed with oral leukoplakia were included in the study. Sociodemographic, clinicopathologic and lifestyle data, fresh tissue and formalin fixed paraffin embedded (FFPE) tissue samples were collected. The fresh tissue was stored at -80 °C and then submitted to further viral DNA detection by the Linear Array method. Immunohistochemical analysis for the p16INK4a expression was performed. Results: Of the 50 patients included in the study, 62 % were men, and the age ranged from 25 to 82 years. Twenty-three (46 %) were elderly, 46 % were middle-aged adults, and only 12 % were young adults. Most patients were smokers (76 %), 14 % were former smokers, and 10 % were non-smokers. Most patients (56 %) were current drinkers, 22 % were ex-drinkers and 22 % were non-drinkers. Thirty-two percent of the lesions presented some degree of dysplasia. No lesions were positive to HPV by Linear Array detection. Thirty (60 %) OL had p16-low immunoexpression and 20 (40 %) had p16-high immunoexpression. Conclusion: HPV was not identified in the population studied. The high p16INK4a immunoexpression is not dependent on HPV in oral leukoplakia. Broader epidemiological studies are required to clarify the geographic variability in the prevalence of HPV in head and neck squamous cell carcinoma and oral potentially malignant lesions.
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spelling High p16INK4a immunoexpression is not HPV dependent in oral leukoplakiaHuman papillomavirusOral leukoplakiap16(INK4A)Polymerase chain reactionObjective: To evaluate the prevalence of HPV DNA detection in fresh tissue from oral leukoplakia by Linear Array assay, and its correlation with p16INK4a immunoexpression in the northwest region of the São Paulo state, Brazil. Patients and methods: Fifty patients diagnosed with oral leukoplakia were included in the study. Sociodemographic, clinicopathologic and lifestyle data, fresh tissue and formalin fixed paraffin embedded (FFPE) tissue samples were collected. The fresh tissue was stored at -80 °C and then submitted to further viral DNA detection by the Linear Array method. Immunohistochemical analysis for the p16INK4a expression was performed. Results: Of the 50 patients included in the study, 62 % were men, and the age ranged from 25 to 82 years. Twenty-three (46 %) were elderly, 46 % were middle-aged adults, and only 12 % were young adults. Most patients were smokers (76 %), 14 % were former smokers, and 10 % were non-smokers. Most patients (56 %) were current drinkers, 22 % were ex-drinkers and 22 % were non-drinkers. Thirty-two percent of the lesions presented some degree of dysplasia. No lesions were positive to HPV by Linear Array detection. Thirty (60 %) OL had p16-low immunoexpression and 20 (40 %) had p16-high immunoexpression. Conclusion: HPV was not identified in the population studied. The high p16INK4a immunoexpression is not dependent on HPV in oral leukoplakia. Broader epidemiological studies are required to clarify the geographic variability in the prevalence of HPV in head and neck squamous cell carcinoma and oral potentially malignant lesions.Oral Oncology Center São Paulo State University (UNESP) School of DentistryDepartment of Diagnosis and Surgery São Paulo State University (UNESP) School of DentistryLaboratory of Pharmacology Department of Basic Sciences São Paulo State University (UNESP) School of DentistryCentre of Translational Oncology Instituto do Câncer do Estado de São Paulo (ICESP)Department of Radiology and Oncology Faculdade de Medicina Universidade de São PauloOral Oncology Center São Paulo State University (UNESP) School of DentistryDepartment of Diagnosis and Surgery São Paulo State University (UNESP) School of DentistryLaboratory of Pharmacology Department of Basic Sciences São Paulo State University (UNESP) School of DentistryUniversidade Estadual Paulista (Unesp)Instituto do Câncer do Estado de São Paulo (ICESP)Universidade de São Paulo (USP)Tomo, Saygo [UNESP]Biss, Stephanye Pinto [UNESP]Crivelini, Marcelo Macedo [UNESP]de Oliveira, Sandra Helena Penha [UNESP]Biasoli, Éder Ricardo [UNESP]Tjioe, Kellen Cristine [UNESP]Bernabé, Daniel Galera [UNESP]Villa, Luisa LinaMiyahara, Glauco Issamu [UNESP]2020-12-12T02:05:31Z2020-12-12T02:05:31Z2020-07-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.1016/j.archoralbio.2020.104738Archives of Oral Biology, v. 115.1879-15060003-9969http://hdl.handle.net/11449/20039810.1016/j.archoralbio.2020.1047382-s2.0-85084513438Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengArchives of Oral Biologyinfo:eu-repo/semantics/openAccess2024-04-11T20:16:33Zoai:repositorio.unesp.br:11449/200398Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T18:42:00.705526Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv High p16INK4a immunoexpression is not HPV dependent in oral leukoplakia
title High p16INK4a immunoexpression is not HPV dependent in oral leukoplakia
spellingShingle High p16INK4a immunoexpression is not HPV dependent in oral leukoplakia
Tomo, Saygo [UNESP]
Human papillomavirus
Oral leukoplakia
p16(INK4A)
Polymerase chain reaction
title_short High p16INK4a immunoexpression is not HPV dependent in oral leukoplakia
title_full High p16INK4a immunoexpression is not HPV dependent in oral leukoplakia
title_fullStr High p16INK4a immunoexpression is not HPV dependent in oral leukoplakia
title_full_unstemmed High p16INK4a immunoexpression is not HPV dependent in oral leukoplakia
title_sort High p16INK4a immunoexpression is not HPV dependent in oral leukoplakia
author Tomo, Saygo [UNESP]
author_facet Tomo, Saygo [UNESP]
Biss, Stephanye Pinto [UNESP]
Crivelini, Marcelo Macedo [UNESP]
de Oliveira, Sandra Helena Penha [UNESP]
Biasoli, Éder Ricardo [UNESP]
Tjioe, Kellen Cristine [UNESP]
Bernabé, Daniel Galera [UNESP]
Villa, Luisa Lina
Miyahara, Glauco Issamu [UNESP]
author_role author
author2 Biss, Stephanye Pinto [UNESP]
Crivelini, Marcelo Macedo [UNESP]
de Oliveira, Sandra Helena Penha [UNESP]
Biasoli, Éder Ricardo [UNESP]
Tjioe, Kellen Cristine [UNESP]
Bernabé, Daniel Galera [UNESP]
Villa, Luisa Lina
Miyahara, Glauco Issamu [UNESP]
author2_role author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade Estadual Paulista (Unesp)
Instituto do Câncer do Estado de São Paulo (ICESP)
Universidade de São Paulo (USP)
dc.contributor.author.fl_str_mv Tomo, Saygo [UNESP]
Biss, Stephanye Pinto [UNESP]
Crivelini, Marcelo Macedo [UNESP]
de Oliveira, Sandra Helena Penha [UNESP]
Biasoli, Éder Ricardo [UNESP]
Tjioe, Kellen Cristine [UNESP]
Bernabé, Daniel Galera [UNESP]
Villa, Luisa Lina
Miyahara, Glauco Issamu [UNESP]
dc.subject.por.fl_str_mv Human papillomavirus
Oral leukoplakia
p16(INK4A)
Polymerase chain reaction
topic Human papillomavirus
Oral leukoplakia
p16(INK4A)
Polymerase chain reaction
description Objective: To evaluate the prevalence of HPV DNA detection in fresh tissue from oral leukoplakia by Linear Array assay, and its correlation with p16INK4a immunoexpression in the northwest region of the São Paulo state, Brazil. Patients and methods: Fifty patients diagnosed with oral leukoplakia were included in the study. Sociodemographic, clinicopathologic and lifestyle data, fresh tissue and formalin fixed paraffin embedded (FFPE) tissue samples were collected. The fresh tissue was stored at -80 °C and then submitted to further viral DNA detection by the Linear Array method. Immunohistochemical analysis for the p16INK4a expression was performed. Results: Of the 50 patients included in the study, 62 % were men, and the age ranged from 25 to 82 years. Twenty-three (46 %) were elderly, 46 % were middle-aged adults, and only 12 % were young adults. Most patients were smokers (76 %), 14 % were former smokers, and 10 % were non-smokers. Most patients (56 %) were current drinkers, 22 % were ex-drinkers and 22 % were non-drinkers. Thirty-two percent of the lesions presented some degree of dysplasia. No lesions were positive to HPV by Linear Array detection. Thirty (60 %) OL had p16-low immunoexpression and 20 (40 %) had p16-high immunoexpression. Conclusion: HPV was not identified in the population studied. The high p16INK4a immunoexpression is not dependent on HPV in oral leukoplakia. Broader epidemiological studies are required to clarify the geographic variability in the prevalence of HPV in head and neck squamous cell carcinoma and oral potentially malignant lesions.
publishDate 2020
dc.date.none.fl_str_mv 2020-12-12T02:05:31Z
2020-12-12T02:05:31Z
2020-07-01
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1016/j.archoralbio.2020.104738
Archives of Oral Biology, v. 115.
1879-1506
0003-9969
http://hdl.handle.net/11449/200398
10.1016/j.archoralbio.2020.104738
2-s2.0-85084513438
url http://dx.doi.org/10.1016/j.archoralbio.2020.104738
http://hdl.handle.net/11449/200398
identifier_str_mv Archives of Oral Biology, v. 115.
1879-1506
0003-9969
10.1016/j.archoralbio.2020.104738
2-s2.0-85084513438
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Archives of Oral Biology
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
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