Evaluation of Headspace Solid-Phase Microextraction Gas Chromatography–Mass Spectrometry for the Characterization of Volatile Organic Compounds from Melon (Cucumis melo L.) Flowers
Autor(a) principal: | |
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Data de Publicação: | 2018 |
Outros Autores: | , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1007/s10337-018-3550-0 http://hdl.handle.net/11449/171110 |
Resumo: | Abstract: The secondary metabolism of plants plays a key role in plant–insect interactions. Among the various classes of metabolites produced, volatile organic compounds (VOCs) play important echophysiological roles, including the attraction of pollinators. This work aimed to develop a method to extract VOCs using headspace (HS) and solid-phase microextraction (SPME), which can be applied in future studies to contribute to the understanding of the role of VOCs in the pollination process. Therefore, a HS–SPME method combined with gas chromatography–mass spectrometry (GC–MS) was developed for the extraction and characterization of VOCs of melon flowers (Cucumis melo L.). The extraction was carried out with the fibers PDMS, DVB/CAR/PDMS and CAR/PDMS for the times 15, 30, and 45 min. Fifty compounds belonging to the following chemical classes were identified: terpenes, hydrocarbons, esters, ethers, ketones, aldehydes, and alcohols. CAR/PDMS fiber extracted the largest amount of compounds, and was more efficient for the extraction of volatile compounds with lower molecular weight, low boiling point and ideal for analytes in low concentration. Therefore, the evaluated method proved to be a promising tool for the analysis of VOCs of melon flowers, to be applied in new studies of the relationships between chemical composition and the processes of attraction of pollinators. Graphical Abstract: [Figure not available: see fulltext.]. |
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Evaluation of Headspace Solid-Phase Microextraction Gas Chromatography–Mass Spectrometry for the Characterization of Volatile Organic Compounds from Melon (Cucumis melo L.) FlowersGC–MSHeadspaceMelon flowerSPMEVOCsAbstract: The secondary metabolism of plants plays a key role in plant–insect interactions. Among the various classes of metabolites produced, volatile organic compounds (VOCs) play important echophysiological roles, including the attraction of pollinators. This work aimed to develop a method to extract VOCs using headspace (HS) and solid-phase microextraction (SPME), which can be applied in future studies to contribute to the understanding of the role of VOCs in the pollination process. Therefore, a HS–SPME method combined with gas chromatography–mass spectrometry (GC–MS) was developed for the extraction and characterization of VOCs of melon flowers (Cucumis melo L.). The extraction was carried out with the fibers PDMS, DVB/CAR/PDMS and CAR/PDMS for the times 15, 30, and 45 min. Fifty compounds belonging to the following chemical classes were identified: terpenes, hydrocarbons, esters, ethers, ketones, aldehydes, and alcohols. CAR/PDMS fiber extracted the largest amount of compounds, and was more efficient for the extraction of volatile compounds with lower molecular weight, low boiling point and ideal for analytes in low concentration. Therefore, the evaluated method proved to be a promising tool for the analysis of VOCs of melon flowers, to be applied in new studies of the relationships between chemical composition and the processes of attraction of pollinators. Graphical Abstract: [Figure not available: see fulltext.].Laboratório Multiusuário de Química de Produtos Naturais-Embrapa Agroindústria Tropical, Rua Dra. Sara Mesquita, 2270-PiciInstitute of Chemistry São Paulo State University (Unesp), Rua Prof. Francisco Degni, 55, Bairro: QuitandinhaLaboratório de Abelhas Departamento de Zootecnia-CCA Universidade Federal do Ceará Campus Universitário do Pici, Bloco 814Curso de Química Centro de Ciências Exatas e Tecnologia Universidade Estadual Vale do Acaraú, Avenida da Universidade 850Departamento de Química Analítica e Físico-Química Centro de Ciências Universidade Federal do CearáInstitute of Chemistry São Paulo State University (Unesp), Rua Prof. Francisco Degni, 55, Bairro: QuitandinhaEmpresa Brasileira de Pesquisa Agropecuária (EMBRAPA)Universidade Estadual Paulista (Unesp)Universidade Federal do CearáUniversidade Estadual Vale do AcaraúSilva, Francisca Aliny Nunesda Silva, Alexander Alves [UNESP]de Sousa Fernandes, NayannyRodrigues, Tigressa Helena SoaresCanuto, Kirley Marquesdo Nascimento, Ronaldo Ferreirade Brito, Edy Sousade Aragão, Fernando Antonio SouzaFreitas, Breno MagalhãesZocolo, Guilherme Julião2018-12-11T16:53:56Z2018-12-11T16:53:56Z2018-08-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article1231-1239application/pdfhttp://dx.doi.org/10.1007/s10337-018-3550-0Chromatographia, v. 81, n. 8, p. 1231-1239, 2018.1612-11120009-5893http://hdl.handle.net/11449/17111010.1007/s10337-018-3550-02-s2.0-850486532152-s2.0-85048653215.pdfScopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengChromatographia0,514info:eu-repo/semantics/openAccess2024-01-03T06:19:03Zoai:repositorio.unesp.br:11449/171110Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-01-03T06:19:03Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Evaluation of Headspace Solid-Phase Microextraction Gas Chromatography–Mass Spectrometry for the Characterization of Volatile Organic Compounds from Melon (Cucumis melo L.) Flowers |
title |
Evaluation of Headspace Solid-Phase Microextraction Gas Chromatography–Mass Spectrometry for the Characterization of Volatile Organic Compounds from Melon (Cucumis melo L.) Flowers |
spellingShingle |
Evaluation of Headspace Solid-Phase Microextraction Gas Chromatography–Mass Spectrometry for the Characterization of Volatile Organic Compounds from Melon (Cucumis melo L.) Flowers Silva, Francisca Aliny Nunes GC–MS Headspace Melon flower SPME VOCs |
title_short |
Evaluation of Headspace Solid-Phase Microextraction Gas Chromatography–Mass Spectrometry for the Characterization of Volatile Organic Compounds from Melon (Cucumis melo L.) Flowers |
title_full |
Evaluation of Headspace Solid-Phase Microextraction Gas Chromatography–Mass Spectrometry for the Characterization of Volatile Organic Compounds from Melon (Cucumis melo L.) Flowers |
title_fullStr |
Evaluation of Headspace Solid-Phase Microextraction Gas Chromatography–Mass Spectrometry for the Characterization of Volatile Organic Compounds from Melon (Cucumis melo L.) Flowers |
title_full_unstemmed |
Evaluation of Headspace Solid-Phase Microextraction Gas Chromatography–Mass Spectrometry for the Characterization of Volatile Organic Compounds from Melon (Cucumis melo L.) Flowers |
title_sort |
Evaluation of Headspace Solid-Phase Microextraction Gas Chromatography–Mass Spectrometry for the Characterization of Volatile Organic Compounds from Melon (Cucumis melo L.) Flowers |
author |
Silva, Francisca Aliny Nunes |
author_facet |
Silva, Francisca Aliny Nunes da Silva, Alexander Alves [UNESP] de Sousa Fernandes, Nayanny Rodrigues, Tigressa Helena Soares Canuto, Kirley Marques do Nascimento, Ronaldo Ferreira de Brito, Edy Sousa de Aragão, Fernando Antonio Souza Freitas, Breno Magalhães Zocolo, Guilherme Julião |
author_role |
author |
author2 |
da Silva, Alexander Alves [UNESP] de Sousa Fernandes, Nayanny Rodrigues, Tigressa Helena Soares Canuto, Kirley Marques do Nascimento, Ronaldo Ferreira de Brito, Edy Sousa de Aragão, Fernando Antonio Souza Freitas, Breno Magalhães Zocolo, Guilherme Julião |
author2_role |
author author author author author author author author author |
dc.contributor.none.fl_str_mv |
Empresa Brasileira de Pesquisa Agropecuária (EMBRAPA) Universidade Estadual Paulista (Unesp) Universidade Federal do Ceará Universidade Estadual Vale do Acaraú |
dc.contributor.author.fl_str_mv |
Silva, Francisca Aliny Nunes da Silva, Alexander Alves [UNESP] de Sousa Fernandes, Nayanny Rodrigues, Tigressa Helena Soares Canuto, Kirley Marques do Nascimento, Ronaldo Ferreira de Brito, Edy Sousa de Aragão, Fernando Antonio Souza Freitas, Breno Magalhães Zocolo, Guilherme Julião |
dc.subject.por.fl_str_mv |
GC–MS Headspace Melon flower SPME VOCs |
topic |
GC–MS Headspace Melon flower SPME VOCs |
description |
Abstract: The secondary metabolism of plants plays a key role in plant–insect interactions. Among the various classes of metabolites produced, volatile organic compounds (VOCs) play important echophysiological roles, including the attraction of pollinators. This work aimed to develop a method to extract VOCs using headspace (HS) and solid-phase microextraction (SPME), which can be applied in future studies to contribute to the understanding of the role of VOCs in the pollination process. Therefore, a HS–SPME method combined with gas chromatography–mass spectrometry (GC–MS) was developed for the extraction and characterization of VOCs of melon flowers (Cucumis melo L.). The extraction was carried out with the fibers PDMS, DVB/CAR/PDMS and CAR/PDMS for the times 15, 30, and 45 min. Fifty compounds belonging to the following chemical classes were identified: terpenes, hydrocarbons, esters, ethers, ketones, aldehydes, and alcohols. CAR/PDMS fiber extracted the largest amount of compounds, and was more efficient for the extraction of volatile compounds with lower molecular weight, low boiling point and ideal for analytes in low concentration. Therefore, the evaluated method proved to be a promising tool for the analysis of VOCs of melon flowers, to be applied in new studies of the relationships between chemical composition and the processes of attraction of pollinators. Graphical Abstract: [Figure not available: see fulltext.]. |
publishDate |
2018 |
dc.date.none.fl_str_mv |
2018-12-11T16:53:56Z 2018-12-11T16:53:56Z 2018-08-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1007/s10337-018-3550-0 Chromatographia, v. 81, n. 8, p. 1231-1239, 2018. 1612-1112 0009-5893 http://hdl.handle.net/11449/171110 10.1007/s10337-018-3550-0 2-s2.0-85048653215 2-s2.0-85048653215.pdf |
url |
http://dx.doi.org/10.1007/s10337-018-3550-0 http://hdl.handle.net/11449/171110 |
identifier_str_mv |
Chromatographia, v. 81, n. 8, p. 1231-1239, 2018. 1612-1112 0009-5893 10.1007/s10337-018-3550-0 2-s2.0-85048653215 2-s2.0-85048653215.pdf |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Chromatographia 0,514 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
1231-1239 application/pdf |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1799965495294689280 |