Re-induction of desiccation tolerance in germinated cowpea seeds
Autor(a) principal: | |
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Data de Publicação: | 2017 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1016/j.sajb.2017.07.011 http://hdl.handle.net/11449/163553 |
Resumo: | The objective of this work was to re-induce desiccation tolerance (DT) in cowpea germinated seeds and to investigate the mechanisms related to the re-induction of DT in this species. For this, germinated seeds with primary roots with length of 1, 2, 3 and 4 mm were treated with osmotic solution of polyethylene glycol 6000 (PEG) of - 1.7 MPa at 10 degrees C for 24, 48 and 72 h. Those seeds were dried, rehydrated and evaluated regarding the reinduction of DT. We studied mitosis events and performed scanning electron microscopy (SEM) in primary roots of untreated and PEG-treated germinated seeds. Furthermore, we quantified the glucose, sucrose and raffinose contents during the re-induction of DT. The re-induction of DT in cowpea germinated seeds with primary roots with length up to 2 mm was successfully obtained through an osmotic treatment of PEG during 24 h. The PEG treatment favored the maintenance of cellular structure integrity, the arrest of cell cycle and the accumulation of sucrose in the primary roots. Those mechanisms may contribute to re-induction DT in germinated cowpea seeds. (C) 2017 SAAB. Published by Elsevier B.V. All rights reserved. |
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Repositório Institucional da UNESP |
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Re-induction of desiccation tolerance in germinated cowpea seedsCell cycleCell ultrastructureNon-reducing sugarOsmotic treatmentVigna unguiculataThe objective of this work was to re-induce desiccation tolerance (DT) in cowpea germinated seeds and to investigate the mechanisms related to the re-induction of DT in this species. For this, germinated seeds with primary roots with length of 1, 2, 3 and 4 mm were treated with osmotic solution of polyethylene glycol 6000 (PEG) of - 1.7 MPa at 10 degrees C for 24, 48 and 72 h. Those seeds were dried, rehydrated and evaluated regarding the reinduction of DT. We studied mitosis events and performed scanning electron microscopy (SEM) in primary roots of untreated and PEG-treated germinated seeds. Furthermore, we quantified the glucose, sucrose and raffinose contents during the re-induction of DT. The re-induction of DT in cowpea germinated seeds with primary roots with length up to 2 mm was successfully obtained through an osmotic treatment of PEG during 24 h. The PEG treatment favored the maintenance of cellular structure integrity, the arrest of cell cycle and the accumulation of sucrose in the primary roots. Those mechanisms may contribute to re-induction DT in germinated cowpea seeds. (C) 2017 SAAB. Published by Elsevier B.V. All rights reserved.Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Univ Estadual Paulista, Dept Prod & Melhoramento Vegetal, Rua Dr Jose Barbosa de Barros 1780, BR-8610307 Botucatu, SP, BrazilUniv Fed Lavras, Dept Biol, Av Cent S-N, Lavras, MG, BrazilUniv Estadual Paulista, Dept Prod & Melhoramento Vegetal, Rua Dr Jose Barbosa de Barros 1780, BR-8610307 Botucatu, SP, BrazilElsevier B.V.Universidade Estadual Paulista (Unesp)Universidade Federal de Lavras (UFLA)Silva, A. C. da [UNESP]Davide, L. C.Braz, G. T.Maia, J. [UNESP]Castro, E. M. deSilva, E. A. A. da [UNESP]2018-11-26T17:42:30Z2018-11-26T17:42:30Z2017-11-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article34-39application/pdfhttp://dx.doi.org/10.1016/j.sajb.2017.07.011South African Journal Of Botany. Amsterdam: Elsevier Science Bv, v. 113, p. 34-39, 2017.0254-6299http://hdl.handle.net/11449/16355310.1016/j.sajb.2017.07.011WOS:000416988100006WOS000416988100006.pdfWeb of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengSouth African Journal Of Botany0,450info:eu-repo/semantics/openAccess2024-04-30T15:58:21Zoai:repositorio.unesp.br:11449/163553Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T21:27:11.356269Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Re-induction of desiccation tolerance in germinated cowpea seeds |
title |
Re-induction of desiccation tolerance in germinated cowpea seeds |
spellingShingle |
Re-induction of desiccation tolerance in germinated cowpea seeds Silva, A. C. da [UNESP] Cell cycle Cell ultrastructure Non-reducing sugar Osmotic treatment Vigna unguiculata |
title_short |
Re-induction of desiccation tolerance in germinated cowpea seeds |
title_full |
Re-induction of desiccation tolerance in germinated cowpea seeds |
title_fullStr |
Re-induction of desiccation tolerance in germinated cowpea seeds |
title_full_unstemmed |
Re-induction of desiccation tolerance in germinated cowpea seeds |
title_sort |
Re-induction of desiccation tolerance in germinated cowpea seeds |
author |
Silva, A. C. da [UNESP] |
author_facet |
Silva, A. C. da [UNESP] Davide, L. C. Braz, G. T. Maia, J. [UNESP] Castro, E. M. de Silva, E. A. A. da [UNESP] |
author_role |
author |
author2 |
Davide, L. C. Braz, G. T. Maia, J. [UNESP] Castro, E. M. de Silva, E. A. A. da [UNESP] |
author2_role |
author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) Universidade Federal de Lavras (UFLA) |
dc.contributor.author.fl_str_mv |
Silva, A. C. da [UNESP] Davide, L. C. Braz, G. T. Maia, J. [UNESP] Castro, E. M. de Silva, E. A. A. da [UNESP] |
dc.subject.por.fl_str_mv |
Cell cycle Cell ultrastructure Non-reducing sugar Osmotic treatment Vigna unguiculata |
topic |
Cell cycle Cell ultrastructure Non-reducing sugar Osmotic treatment Vigna unguiculata |
description |
The objective of this work was to re-induce desiccation tolerance (DT) in cowpea germinated seeds and to investigate the mechanisms related to the re-induction of DT in this species. For this, germinated seeds with primary roots with length of 1, 2, 3 and 4 mm were treated with osmotic solution of polyethylene glycol 6000 (PEG) of - 1.7 MPa at 10 degrees C for 24, 48 and 72 h. Those seeds were dried, rehydrated and evaluated regarding the reinduction of DT. We studied mitosis events and performed scanning electron microscopy (SEM) in primary roots of untreated and PEG-treated germinated seeds. Furthermore, we quantified the glucose, sucrose and raffinose contents during the re-induction of DT. The re-induction of DT in cowpea germinated seeds with primary roots with length up to 2 mm was successfully obtained through an osmotic treatment of PEG during 24 h. The PEG treatment favored the maintenance of cellular structure integrity, the arrest of cell cycle and the accumulation of sucrose in the primary roots. Those mechanisms may contribute to re-induction DT in germinated cowpea seeds. (C) 2017 SAAB. Published by Elsevier B.V. All rights reserved. |
publishDate |
2017 |
dc.date.none.fl_str_mv |
2017-11-01 2018-11-26T17:42:30Z 2018-11-26T17:42:30Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1016/j.sajb.2017.07.011 South African Journal Of Botany. Amsterdam: Elsevier Science Bv, v. 113, p. 34-39, 2017. 0254-6299 http://hdl.handle.net/11449/163553 10.1016/j.sajb.2017.07.011 WOS:000416988100006 WOS000416988100006.pdf |
url |
http://dx.doi.org/10.1016/j.sajb.2017.07.011 http://hdl.handle.net/11449/163553 |
identifier_str_mv |
South African Journal Of Botany. Amsterdam: Elsevier Science Bv, v. 113, p. 34-39, 2017. 0254-6299 10.1016/j.sajb.2017.07.011 WOS:000416988100006 WOS000416988100006.pdf |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
South African Journal Of Botany 0,450 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
34-39 application/pdf |
dc.publisher.none.fl_str_mv |
Elsevier B.V. |
publisher.none.fl_str_mv |
Elsevier B.V. |
dc.source.none.fl_str_mv |
Web of Science reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808129321227780096 |