Circulating tumor cell isolation from clinical samples utilizing a lateral filter array microfluidic device

Detalhes bibliográficos
Autor(a) principal: Dopico, Pablo J.
Data de Publicação: 2019
Outros Autores: Chen, Kangfu, Varillas, Jose, Pedrosa, Valber [UNESP], George, Thomas J., Hugh Fan, Z.
Tipo de documento: Artigo de conferência
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://hdl.handle.net/11449/221602
Resumo: Isolation methods of circulating tumor cells (CTCs) usually rely on either immunoaffinity or size-based separation. However, immunoaffinity methods miss CTCs under-expressing surface markers of interest. Size-based methods miss CTCs with a size less than the filter geometry designed. We have developed a lateral filter array microfluidic (LFAM) device that integrates size-based separation with immunoaffinity-based isolation. We have demonstrated that the LFAM device improves CTC capture from blood samples of colorectal cancer patients when compared to a microfluidic device using either size-based filtration only or immunoaffinity alone.
id UNSP_b38b13731329dc730aea775a63d51618
oai_identifier_str oai:repositorio.unesp.br:11449/221602
network_acronym_str UNSP
network_name_str Repositório Institucional da UNESP
repository_id_str 2946
spelling Circulating tumor cell isolation from clinical samples utilizing a lateral filter array microfluidic deviceCirculating tumor cellsColorectal CancerFiltrationImmunoaffinityIsolation methods of circulating tumor cells (CTCs) usually rely on either immunoaffinity or size-based separation. However, immunoaffinity methods miss CTCs under-expressing surface markers of interest. Size-based methods miss CTCs with a size less than the filter geometry designed. We have developed a lateral filter array microfluidic (LFAM) device that integrates size-based separation with immunoaffinity-based isolation. We have demonstrated that the LFAM device improves CTC capture from blood samples of colorectal cancer patients when compared to a microfluidic device using either size-based filtration only or immunoaffinity alone.University of FloridaPatton Charitable FoundationUniversity of Florida HealthDepartment of Mechanical and Aerospace Engineering University of FloridaPruitt Family Department of Biomedical Engineering University of FloridaInstitute of Biosciences São Paulo State UniversityDepartment of Medicine University of FloridaInstitute of Biosciences São Paulo State UniversityUniversity of FloridaUniversidade Estadual Paulista (UNESP)Dopico, Pablo J.Chen, KangfuVarillas, JosePedrosa, Valber [UNESP]George, Thomas J.Hugh Fan, Z.2022-04-28T19:29:43Z2022-04-28T19:29:43Z2019-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObject1514-151523rd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2019, p. 1514-1515.http://hdl.handle.net/11449/2216022-s2.0-85094977897Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPeng23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2019info:eu-repo/semantics/openAccess2022-04-28T19:29:43Zoai:repositorio.unesp.br:11449/221602Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T19:00:57.011149Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Circulating tumor cell isolation from clinical samples utilizing a lateral filter array microfluidic device
title Circulating tumor cell isolation from clinical samples utilizing a lateral filter array microfluidic device
spellingShingle Circulating tumor cell isolation from clinical samples utilizing a lateral filter array microfluidic device
Dopico, Pablo J.
Circulating tumor cells
Colorectal Cancer
Filtration
Immunoaffinity
title_short Circulating tumor cell isolation from clinical samples utilizing a lateral filter array microfluidic device
title_full Circulating tumor cell isolation from clinical samples utilizing a lateral filter array microfluidic device
title_fullStr Circulating tumor cell isolation from clinical samples utilizing a lateral filter array microfluidic device
title_full_unstemmed Circulating tumor cell isolation from clinical samples utilizing a lateral filter array microfluidic device
title_sort Circulating tumor cell isolation from clinical samples utilizing a lateral filter array microfluidic device
author Dopico, Pablo J.
author_facet Dopico, Pablo J.
Chen, Kangfu
Varillas, Jose
Pedrosa, Valber [UNESP]
George, Thomas J.
Hugh Fan, Z.
author_role author
author2 Chen, Kangfu
Varillas, Jose
Pedrosa, Valber [UNESP]
George, Thomas J.
Hugh Fan, Z.
author2_role author
author
author
author
author
dc.contributor.none.fl_str_mv University of Florida
Universidade Estadual Paulista (UNESP)
dc.contributor.author.fl_str_mv Dopico, Pablo J.
Chen, Kangfu
Varillas, Jose
Pedrosa, Valber [UNESP]
George, Thomas J.
Hugh Fan, Z.
dc.subject.por.fl_str_mv Circulating tumor cells
Colorectal Cancer
Filtration
Immunoaffinity
topic Circulating tumor cells
Colorectal Cancer
Filtration
Immunoaffinity
description Isolation methods of circulating tumor cells (CTCs) usually rely on either immunoaffinity or size-based separation. However, immunoaffinity methods miss CTCs under-expressing surface markers of interest. Size-based methods miss CTCs with a size less than the filter geometry designed. We have developed a lateral filter array microfluidic (LFAM) device that integrates size-based separation with immunoaffinity-based isolation. We have demonstrated that the LFAM device improves CTC capture from blood samples of colorectal cancer patients when compared to a microfluidic device using either size-based filtration only or immunoaffinity alone.
publishDate 2019
dc.date.none.fl_str_mv 2019-01-01
2022-04-28T19:29:43Z
2022-04-28T19:29:43Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/conferenceObject
format conferenceObject
status_str publishedVersion
dc.identifier.uri.fl_str_mv 23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2019, p. 1514-1515.
http://hdl.handle.net/11449/221602
2-s2.0-85094977897
identifier_str_mv 23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2019, p. 1514-1515.
2-s2.0-85094977897
url http://hdl.handle.net/11449/221602
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2019
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 1514-1515
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
_version_ 1808129011782516736

Registros relacionados