Clinical validation of a size-based microfluidic device for circulating tumor cell isolation and analysis in renal cell carcinoma
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2023 |
| Outros Autores: | , , , , , , , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
| Texto Completo: | http://hdl.handle.net/10451/57695 |
Resumo: | © 2023 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
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Clinical validation of a size-based microfluidic device for circulating tumor cell isolation and analysis in renal cell carcinomaCirculating tumor cellKidney cancerLiquid biopsyMicrofluidicRenal cell carcinoma© 2023 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).Renal cell carcinoma (RCC) presents as metastatic disease in one third of cases. Research on circulating tumor cells (CTCs) and liquid biopsies is improving the understanding of RCC biology and metastases formation. However, a standardized, sensitive, specific, and cost-effective CTC detection technique is lacking. The use of platforms solely relying on epithelial markers is inappropriate in RCC due to the frequent epithelial-mesenchymal transition that CTCs undergo. This study aimed to test and clinically validate RUBYchip™, a microfluidic label-free CTC detection platform, in RCC patients. The average CTC capture efficiency of the device was 74.9% in spiking experiments using three different RCC cell lines. Clinical validation was performed in a cohort of 18 patients, eight non-metastatic (M0), five metastatic treatment-naïve (M1TN), and five metastatic progressing-under-treatment (M1TP). An average CTC detection rate of 77.8% was found and the average (range) total CTC count was 6.4 (0-27), 101.8 (0-255), and 3.2 (0-10), and the average mesenchymal CTC count (both single and clustered cells) was zero, 97.6 (0-255), and 0.2 (0-1) for M0, M1TN, and M1TP, respectively. CTC clusters were detected in 25% and 60% of M0 and M1TN patients, respectively. These results show that RUBYchip™ is an effective CTC detection platform in RCC.MDPIRepositório da Universidade de LisboaPalmela Leitão, TitoCorredeira, PatríciaKucharczak, SandraRodrigues, MargaridaPiairo, PaulinaRodrigues, CarolinaAlves, PatríciaMartins Cavaco, Ana CláudiaMiranda, MiguelAntunes, MariliaFerreira, JoãoPalma Dos Reis, José ManuelLopes, ToméDiéguez, LorenaCosta, Luis2023-05-30T14:22:45Z20232023-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10451/57695engInt J Mol Sci. 2023 May 7;24(9):84041661-659610.3390/ijms240984041422-0067info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-11-08T17:06:29Zoai:repositorio.ul.pt:10451/57695Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T22:08:13.063464Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
| dc.title.none.fl_str_mv |
Clinical validation of a size-based microfluidic device for circulating tumor cell isolation and analysis in renal cell carcinoma |
| title |
Clinical validation of a size-based microfluidic device for circulating tumor cell isolation and analysis in renal cell carcinoma |
| spellingShingle |
Clinical validation of a size-based microfluidic device for circulating tumor cell isolation and analysis in renal cell carcinoma Palmela Leitão, Tito Circulating tumor cell Kidney cancer Liquid biopsy Microfluidic Renal cell carcinoma |
| title_short |
Clinical validation of a size-based microfluidic device for circulating tumor cell isolation and analysis in renal cell carcinoma |
| title_full |
Clinical validation of a size-based microfluidic device for circulating tumor cell isolation and analysis in renal cell carcinoma |
| title_fullStr |
Clinical validation of a size-based microfluidic device for circulating tumor cell isolation and analysis in renal cell carcinoma |
| title_full_unstemmed |
Clinical validation of a size-based microfluidic device for circulating tumor cell isolation and analysis in renal cell carcinoma |
| title_sort |
Clinical validation of a size-based microfluidic device for circulating tumor cell isolation and analysis in renal cell carcinoma |
| author |
Palmela Leitão, Tito |
| author_facet |
Palmela Leitão, Tito Corredeira, Patrícia Kucharczak, Sandra Rodrigues, Margarida Piairo, Paulina Rodrigues, Carolina Alves, Patrícia Martins Cavaco, Ana Cláudia Miranda, Miguel Antunes, Marilia Ferreira, João Palma Dos Reis, José Manuel Lopes, Tomé Diéguez, Lorena Costa, Luis |
| author_role |
author |
| author2 |
Corredeira, Patrícia Kucharczak, Sandra Rodrigues, Margarida Piairo, Paulina Rodrigues, Carolina Alves, Patrícia Martins Cavaco, Ana Cláudia Miranda, Miguel Antunes, Marilia Ferreira, João Palma Dos Reis, José Manuel Lopes, Tomé Diéguez, Lorena Costa, Luis |
| author2_role |
author author author author author author author author author author author author author author |
| dc.contributor.none.fl_str_mv |
Repositório da Universidade de Lisboa |
| dc.contributor.author.fl_str_mv |
Palmela Leitão, Tito Corredeira, Patrícia Kucharczak, Sandra Rodrigues, Margarida Piairo, Paulina Rodrigues, Carolina Alves, Patrícia Martins Cavaco, Ana Cláudia Miranda, Miguel Antunes, Marilia Ferreira, João Palma Dos Reis, José Manuel Lopes, Tomé Diéguez, Lorena Costa, Luis |
| dc.subject.por.fl_str_mv |
Circulating tumor cell Kidney cancer Liquid biopsy Microfluidic Renal cell carcinoma |
| topic |
Circulating tumor cell Kidney cancer Liquid biopsy Microfluidic Renal cell carcinoma |
| description |
© 2023 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
| publishDate |
2023 |
| dc.date.none.fl_str_mv |
2023-05-30T14:22:45Z 2023 2023-01-01T00:00:00Z |
| dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
| dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
| format |
article |
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publishedVersion |
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http://hdl.handle.net/10451/57695 |
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http://hdl.handle.net/10451/57695 |
| dc.language.iso.fl_str_mv |
eng |
| language |
eng |
| dc.relation.none.fl_str_mv |
Int J Mol Sci. 2023 May 7;24(9):8404 1661-6596 10.3390/ijms24098404 1422-0067 |
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info:eu-repo/semantics/openAccess |
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openAccess |
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application/pdf |
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MDPI |
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MDPI |
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reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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RCAAP |
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RCAAP |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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