The evolutionary dynamics of the Helena retrotransposon revealed by sequenced Drosophila genomes

Detalhes bibliográficos
Autor(a) principal: Granzotto, Adriana [UNESP]
Data de Publicação: 2009
Outros Autores: Lopes, Fabricio R. [UNESP], Lerat, Emmanuelle, Vieira, Cristina, Carareto, Claudia M. A. [UNESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1186/1471-2148-9-174
http://hdl.handle.net/11449/21646
Resumo: Background: Several studies have shown that genomes contain a mixture of transposable elements, some of which are still active and others ancient relics that have degenerated. This is true for the non-LTR retrotransposon Helena, of which only degenerate sequences have been shown to be present in some species (Drosophila melanogaster), whereas putatively active sequences are present in others (D. simulans). Combining experimental and population analyses with the sequence analysis of the 12 Drosophila genomes, we have investigated the evolution of Helena, and propose a possible scenario for the evolution of this element.Results: We show that six species of Drosophila have the Helena transposable element at different stages of its evolution. The copy number is highly variable among these species, but most of them are truncated at the 5' ends and also harbor several internal deletions and insertions suggesting that they are inactive in all species, except in D. mojavensis in which quantitative RT-PCR experiments have identified a putative active copy.Conclusion: Our data suggest that Helena was present in the common ancestor of the Drosophila genus, which has been vertically transmitted to the derived lineages, but that it has been lost in some of them. The wide variation in copy number and sequence degeneration in the different species suggest that the evolutionary dynamics of Helena depends on the genomic environment of the host species.
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spelling The evolutionary dynamics of the Helena retrotransposon revealed by sequenced Drosophila genomesBackground: Several studies have shown that genomes contain a mixture of transposable elements, some of which are still active and others ancient relics that have degenerated. This is true for the non-LTR retrotransposon Helena, of which only degenerate sequences have been shown to be present in some species (Drosophila melanogaster), whereas putatively active sequences are present in others (D. simulans). Combining experimental and population analyses with the sequence analysis of the 12 Drosophila genomes, we have investigated the evolution of Helena, and propose a possible scenario for the evolution of this element.Results: We show that six species of Drosophila have the Helena transposable element at different stages of its evolution. The copy number is highly variable among these species, but most of them are truncated at the 5' ends and also harbor several internal deletions and insertions suggesting that they are inactive in all species, except in D. mojavensis in which quantitative RT-PCR experiments have identified a putative active copy.Conclusion: Our data suggest that Helena was present in the common ancestor of the Drosophila genus, which has been vertically transmitted to the derived lineages, but that it has been lost in some of them. The wide variation in copy number and sequence degeneration in the different species suggest that the evolutionary dynamics of Helena depends on the genomic environment of the host species.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)French MIRA project from the Region Rhone-AlpesANR GenemobileUNESP, Lab Mol Evolut, Dept Biol, BR-15054000 Sao Jose do Rio Preto, SP, BrazilUniv Lyon, F-69000 Lyon, FranceUniv Lyon 1, CNRS, UMR5558, Lab Biometrie & Biol Evolut, F-69622 Villeurbanne, FranceUNESP, Lab Mol Evolut, Dept Biol, BR-15054000 Sao Jose do Rio Preto, SP, BrazilFAPESP: 07/53097-0FAPESP: 07/50641-1Biomed Central Ltd.Universidade Estadual Paulista (Unesp)Univ LyonUniv Lyon 1Granzotto, Adriana [UNESP]Lopes, Fabricio R. [UNESP]Lerat, EmmanuelleVieira, CristinaCarareto, Claudia M. A. [UNESP]2014-05-20T14:01:15Z2014-05-20T14:01:15Z2009-07-22info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article11application/pdfhttp://dx.doi.org/10.1186/1471-2148-9-174Bmc Evolutionary Biology. London: Biomed Central Ltd., v. 9, p. 11, 2009.1471-2148http://hdl.handle.net/11449/2164610.1186/1471-2148-9-174WOS:000269469100003WOS000269469100003.pdf34257729983192160000-0002-0298-1354Web of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengBMC Evolutionary Biology3.0271,656info:eu-repo/semantics/openAccess2023-12-04T06:15:40Zoai:repositorio.unesp.br:11449/21646Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T19:29:12.743015Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv The evolutionary dynamics of the Helena retrotransposon revealed by sequenced Drosophila genomes
title The evolutionary dynamics of the Helena retrotransposon revealed by sequenced Drosophila genomes
spellingShingle The evolutionary dynamics of the Helena retrotransposon revealed by sequenced Drosophila genomes
Granzotto, Adriana [UNESP]
title_short The evolutionary dynamics of the Helena retrotransposon revealed by sequenced Drosophila genomes
title_full The evolutionary dynamics of the Helena retrotransposon revealed by sequenced Drosophila genomes
title_fullStr The evolutionary dynamics of the Helena retrotransposon revealed by sequenced Drosophila genomes
title_full_unstemmed The evolutionary dynamics of the Helena retrotransposon revealed by sequenced Drosophila genomes
title_sort The evolutionary dynamics of the Helena retrotransposon revealed by sequenced Drosophila genomes
author Granzotto, Adriana [UNESP]
author_facet Granzotto, Adriana [UNESP]
Lopes, Fabricio R. [UNESP]
Lerat, Emmanuelle
Vieira, Cristina
Carareto, Claudia M. A. [UNESP]
author_role author
author2 Lopes, Fabricio R. [UNESP]
Lerat, Emmanuelle
Vieira, Cristina
Carareto, Claudia M. A. [UNESP]
author2_role author
author
author
author
dc.contributor.none.fl_str_mv Universidade Estadual Paulista (Unesp)
Univ Lyon
Univ Lyon 1
dc.contributor.author.fl_str_mv Granzotto, Adriana [UNESP]
Lopes, Fabricio R. [UNESP]
Lerat, Emmanuelle
Vieira, Cristina
Carareto, Claudia M. A. [UNESP]
description Background: Several studies have shown that genomes contain a mixture of transposable elements, some of which are still active and others ancient relics that have degenerated. This is true for the non-LTR retrotransposon Helena, of which only degenerate sequences have been shown to be present in some species (Drosophila melanogaster), whereas putatively active sequences are present in others (D. simulans). Combining experimental and population analyses with the sequence analysis of the 12 Drosophila genomes, we have investigated the evolution of Helena, and propose a possible scenario for the evolution of this element.Results: We show that six species of Drosophila have the Helena transposable element at different stages of its evolution. The copy number is highly variable among these species, but most of them are truncated at the 5' ends and also harbor several internal deletions and insertions suggesting that they are inactive in all species, except in D. mojavensis in which quantitative RT-PCR experiments have identified a putative active copy.Conclusion: Our data suggest that Helena was present in the common ancestor of the Drosophila genus, which has been vertically transmitted to the derived lineages, but that it has been lost in some of them. The wide variation in copy number and sequence degeneration in the different species suggest that the evolutionary dynamics of Helena depends on the genomic environment of the host species.
publishDate 2009
dc.date.none.fl_str_mv 2009-07-22
2014-05-20T14:01:15Z
2014-05-20T14:01:15Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1186/1471-2148-9-174
Bmc Evolutionary Biology. London: Biomed Central Ltd., v. 9, p. 11, 2009.
1471-2148
http://hdl.handle.net/11449/21646
10.1186/1471-2148-9-174
WOS:000269469100003
WOS000269469100003.pdf
3425772998319216
0000-0002-0298-1354
url http://dx.doi.org/10.1186/1471-2148-9-174
http://hdl.handle.net/11449/21646
identifier_str_mv Bmc Evolutionary Biology. London: Biomed Central Ltd., v. 9, p. 11, 2009.
1471-2148
10.1186/1471-2148-9-174
WOS:000269469100003
WOS000269469100003.pdf
3425772998319216
0000-0002-0298-1354
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv BMC Evolutionary Biology
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dc.format.none.fl_str_mv 11
application/pdf
dc.publisher.none.fl_str_mv Biomed Central Ltd.
publisher.none.fl_str_mv Biomed Central Ltd.
dc.source.none.fl_str_mv Web of Science
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
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reponame_str Repositório Institucional da UNESP
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repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
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