Low-level laser and antimicrobial photodynamic therapy on experimental periodontitis in rats submitted to chemotherapy by 5-fluorouracil
Autor(a) principal: | |
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Data de Publicação: | 2017 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1007/s00520-017-3738-0 http://hdl.handle.net/11449/178858 |
Resumo: | Purpose: The aim of this study was to evaluate the effects of low-level laser therapy (LLLT) and antimicrobial photodynamic therapy (aPDT) as adjuvant to mechanical treatment of experimental periodontitis (EP) in adult rats submitted to 5-fluorouracil (5-FU) chemotherapy. Methods: EP was induced through ligature around the left mandibular first molar for 7 days. The ligature was removed and the animals separated into groups: EP, no treatment; 5FU, systemic administration of 5-FU (80 and 40 mg/kg); 5FU/scaling and root planing (SRP), systemic application of 5-FU and SRP; 5FU/SRP/LLLT, systemic application of 5-FU, SRP, and LLLT (660 nm, 0.035 W; 29.4 J/cm2); and 5FU/SRP/aPDT, systemic application of 5-FU, SRP, and aPDT (methylene blue irrigation and LLLT). The animals were euthanized 7, 15, and 30 days after treatments. Histological sections from mandibles were processed for histomorphometric and immunohistochemical analysis (TRAP, RANKL, OPG, TNF-α, IL-6, IL-10). The alveolar bone loss (BL) area in the furcation region of the mandibular first molar was analyzed histometrically. Results: There was less bone loss in 5FU/SRP/aPDT compared with 5FU at 7 days (p < 0.05). The immunohistochemical analysis showed no significant difference for TRAP and osteoprotegerin, but lower RANKL immunolabeling was observed in the 5FU/SRP/LLLT and 5FU/SRP/aPDT groups compared with the 5FU group at 15 days. There was lower TNF-α and IL-6 immunolabeling in the 5FU/SRP/LLLT and 5FU/SRP/aPDT groups and higher IL-10 immunolabeling in 5FU/SRP/aPDT at 30 days. Conclusion: LLLT and aPDT adjuvant to SRP minimized the effects of 5-FU on periodontal disease. Furthermore, aPDT promoted greater benefits in bone loss control and inflammatory response. |
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Low-level laser and antimicrobial photodynamic therapy on experimental periodontitis in rats submitted to chemotherapy by 5-fluorouracilChemotherapyLasersPeriodontitisPhotochemotherapyPurpose: The aim of this study was to evaluate the effects of low-level laser therapy (LLLT) and antimicrobial photodynamic therapy (aPDT) as adjuvant to mechanical treatment of experimental periodontitis (EP) in adult rats submitted to 5-fluorouracil (5-FU) chemotherapy. Methods: EP was induced through ligature around the left mandibular first molar for 7 days. The ligature was removed and the animals separated into groups: EP, no treatment; 5FU, systemic administration of 5-FU (80 and 40 mg/kg); 5FU/scaling and root planing (SRP), systemic application of 5-FU and SRP; 5FU/SRP/LLLT, systemic application of 5-FU, SRP, and LLLT (660 nm, 0.035 W; 29.4 J/cm2); and 5FU/SRP/aPDT, systemic application of 5-FU, SRP, and aPDT (methylene blue irrigation and LLLT). The animals were euthanized 7, 15, and 30 days after treatments. Histological sections from mandibles were processed for histomorphometric and immunohistochemical analysis (TRAP, RANKL, OPG, TNF-α, IL-6, IL-10). The alveolar bone loss (BL) area in the furcation region of the mandibular first molar was analyzed histometrically. Results: There was less bone loss in 5FU/SRP/aPDT compared with 5FU at 7 days (p < 0.05). The immunohistochemical analysis showed no significant difference for TRAP and osteoprotegerin, but lower RANKL immunolabeling was observed in the 5FU/SRP/LLLT and 5FU/SRP/aPDT groups compared with the 5FU group at 15 days. There was lower TNF-α and IL-6 immunolabeling in the 5FU/SRP/LLLT and 5FU/SRP/aPDT groups and higher IL-10 immunolabeling in 5FU/SRP/aPDT at 30 days. Conclusion: LLLT and aPDT adjuvant to SRP minimized the effects of 5-FU on periodontal disease. Furthermore, aPDT promoted greater benefits in bone loss control and inflammatory response.Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Division of Periodontology Department of Surgery and Integrated Clinic School of Dentistry São Paulo State University (Unesp)Division of Histology and Embryology Department of Basic Science School of Dentistry São Paulo State University (Unesp)Division of Periodontology Department of Surgery and Integrated Clinic School of Dentistry São Paulo State University (Unesp)Division of Histology and Embryology Department of Basic Science School of Dentistry São Paulo State University (Unesp)CNPq: 2309447-2010-0Universidade Estadual Paulista (Unesp)Theodoro, Leticia Helena [UNESP]Longo, Mariéllen [UNESP]Novaes, Vivian Cristina Noronha [UNESP]Miessi, Daniela Maria Janjacomo [UNESP]Ferro-Alves, Marcio Luiz [UNESP]Ervolino, Edilson [UNESP]de Almeida, Juliano Milanezi [UNESP]Garcia, Valdir Gouveia [UNESP]2018-12-11T17:32:26Z2018-12-11T17:32:26Z2017-10-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article3261-3271application/pdfhttp://dx.doi.org/10.1007/s00520-017-3738-0Supportive Care in Cancer, v. 25, n. 10, p. 3261-3271, 2017.1433-73390941-4355http://hdl.handle.net/11449/17885810.1007/s00520-017-3738-02-s2.0-850190787762-s2.0-85019078776.pdf44080955173468460000-0003-4859-0583Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengSupportive Care in Cancer1,2971,297info:eu-repo/semantics/openAccess2023-10-10T06:04:48Zoai:repositorio.unesp.br:11449/178858Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462023-10-10T06:04:48Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Low-level laser and antimicrobial photodynamic therapy on experimental periodontitis in rats submitted to chemotherapy by 5-fluorouracil |
title |
Low-level laser and antimicrobial photodynamic therapy on experimental periodontitis in rats submitted to chemotherapy by 5-fluorouracil |
spellingShingle |
Low-level laser and antimicrobial photodynamic therapy on experimental periodontitis in rats submitted to chemotherapy by 5-fluorouracil Theodoro, Leticia Helena [UNESP] Chemotherapy Lasers Periodontitis Photochemotherapy |
title_short |
Low-level laser and antimicrobial photodynamic therapy on experimental periodontitis in rats submitted to chemotherapy by 5-fluorouracil |
title_full |
Low-level laser and antimicrobial photodynamic therapy on experimental periodontitis in rats submitted to chemotherapy by 5-fluorouracil |
title_fullStr |
Low-level laser and antimicrobial photodynamic therapy on experimental periodontitis in rats submitted to chemotherapy by 5-fluorouracil |
title_full_unstemmed |
Low-level laser and antimicrobial photodynamic therapy on experimental periodontitis in rats submitted to chemotherapy by 5-fluorouracil |
title_sort |
Low-level laser and antimicrobial photodynamic therapy on experimental periodontitis in rats submitted to chemotherapy by 5-fluorouracil |
author |
Theodoro, Leticia Helena [UNESP] |
author_facet |
Theodoro, Leticia Helena [UNESP] Longo, Mariéllen [UNESP] Novaes, Vivian Cristina Noronha [UNESP] Miessi, Daniela Maria Janjacomo [UNESP] Ferro-Alves, Marcio Luiz [UNESP] Ervolino, Edilson [UNESP] de Almeida, Juliano Milanezi [UNESP] Garcia, Valdir Gouveia [UNESP] |
author_role |
author |
author2 |
Longo, Mariéllen [UNESP] Novaes, Vivian Cristina Noronha [UNESP] Miessi, Daniela Maria Janjacomo [UNESP] Ferro-Alves, Marcio Luiz [UNESP] Ervolino, Edilson [UNESP] de Almeida, Juliano Milanezi [UNESP] Garcia, Valdir Gouveia [UNESP] |
author2_role |
author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Theodoro, Leticia Helena [UNESP] Longo, Mariéllen [UNESP] Novaes, Vivian Cristina Noronha [UNESP] Miessi, Daniela Maria Janjacomo [UNESP] Ferro-Alves, Marcio Luiz [UNESP] Ervolino, Edilson [UNESP] de Almeida, Juliano Milanezi [UNESP] Garcia, Valdir Gouveia [UNESP] |
dc.subject.por.fl_str_mv |
Chemotherapy Lasers Periodontitis Photochemotherapy |
topic |
Chemotherapy Lasers Periodontitis Photochemotherapy |
description |
Purpose: The aim of this study was to evaluate the effects of low-level laser therapy (LLLT) and antimicrobial photodynamic therapy (aPDT) as adjuvant to mechanical treatment of experimental periodontitis (EP) in adult rats submitted to 5-fluorouracil (5-FU) chemotherapy. Methods: EP was induced through ligature around the left mandibular first molar for 7 days. The ligature was removed and the animals separated into groups: EP, no treatment; 5FU, systemic administration of 5-FU (80 and 40 mg/kg); 5FU/scaling and root planing (SRP), systemic application of 5-FU and SRP; 5FU/SRP/LLLT, systemic application of 5-FU, SRP, and LLLT (660 nm, 0.035 W; 29.4 J/cm2); and 5FU/SRP/aPDT, systemic application of 5-FU, SRP, and aPDT (methylene blue irrigation and LLLT). The animals were euthanized 7, 15, and 30 days after treatments. Histological sections from mandibles were processed for histomorphometric and immunohistochemical analysis (TRAP, RANKL, OPG, TNF-α, IL-6, IL-10). The alveolar bone loss (BL) area in the furcation region of the mandibular first molar was analyzed histometrically. Results: There was less bone loss in 5FU/SRP/aPDT compared with 5FU at 7 days (p < 0.05). The immunohistochemical analysis showed no significant difference for TRAP and osteoprotegerin, but lower RANKL immunolabeling was observed in the 5FU/SRP/LLLT and 5FU/SRP/aPDT groups compared with the 5FU group at 15 days. There was lower TNF-α and IL-6 immunolabeling in the 5FU/SRP/LLLT and 5FU/SRP/aPDT groups and higher IL-10 immunolabeling in 5FU/SRP/aPDT at 30 days. Conclusion: LLLT and aPDT adjuvant to SRP minimized the effects of 5-FU on periodontal disease. Furthermore, aPDT promoted greater benefits in bone loss control and inflammatory response. |
publishDate |
2017 |
dc.date.none.fl_str_mv |
2017-10-01 2018-12-11T17:32:26Z 2018-12-11T17:32:26Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1007/s00520-017-3738-0 Supportive Care in Cancer, v. 25, n. 10, p. 3261-3271, 2017. 1433-7339 0941-4355 http://hdl.handle.net/11449/178858 10.1007/s00520-017-3738-0 2-s2.0-85019078776 2-s2.0-85019078776.pdf 4408095517346846 0000-0003-4859-0583 |
url |
http://dx.doi.org/10.1007/s00520-017-3738-0 http://hdl.handle.net/11449/178858 |
identifier_str_mv |
Supportive Care in Cancer, v. 25, n. 10, p. 3261-3271, 2017. 1433-7339 0941-4355 10.1007/s00520-017-3738-0 2-s2.0-85019078776 2-s2.0-85019078776.pdf 4408095517346846 0000-0003-4859-0583 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Supportive Care in Cancer 1,297 1,297 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
3261-3271 application/pdf |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1799964506870251520 |