The role of nanohydroxyapatite on the morphological, physical, and biological properties of chitosan nanofibers

Detalhes bibliográficos
Autor(a) principal: Sato, Tabata P. [UNESP]
Data de Publicação: 2021
Outros Autores: Rodrigues, Bruno V. M., Mello, Daphne C. R. [UNESP], Münchow, Eliseu A., Ribeiro, Juliana S., Machado, João Paulo B., Vasconcellos, Luana M. R. [UNESP], Lobo, Anderson O., Bottino, Marco C., Borges, Alexandre L. S. [UNESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1007/s00784-020-03633-6
http://hdl.handle.net/11449/205300
Resumo: Objectives: This study aimed to evaluate the effects of nanohydroxyapatite (nHAp) particles on the morphological, chemical, physical, and biological properties of chitosan electrospun nanofibers. Materials and methods: nHAp particles with a 1.67 Ca/P ratio were synthesized via the aqueous precipitation method, incorporated into chitosan polymer solution (0.5 wt%), and electrospun into nHAp-loaded fibers (ChHa fibers). Neat chitosan fibers (nHAp-free, Ch fibers) were used as the control. The electrospun fiber mats were characterized using morphological, topographical, chemical, thermal, and a range of biological (antibacterial, antibiofilm, cell viability, and alkaline phosphatase [ALP] activity) analyses. Data were analyzed using ANOVA and Tukey’s test (α = 0.05). Results: ChHa fibers demonstrated a bead-like morphology, with thinner (331 ± 110 nm) and smoother (Ra = 2.9 ± 0.3 μm) distribution as compared to the control fibers. Despite showing similar cell viability and ALP activity to Ch fibers, the ChHa fibers demonstrated greater antibacterial potential against most tested bacteria (except for P. intermedia), and higher antibiofilm activity against P. gingivalis biofilm. Conclusions: The incorporation of nHAp particles did not jeopardize the overall morphology, topography, physical, and biological characteristics of the chitosan nanofibers. Clinical relevance: The combination of nHAp particles with chitosan can be used to engineer bioactive, electrospun composite nanofibers with potential applications in regenerative dentistry.
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spelling The role of nanohydroxyapatite on the morphological, physical, and biological properties of chitosan nanofibersAntimicrobialChitosanElectrospinningRegenerationRegenerative dentistryScaffoldsObjectives: This study aimed to evaluate the effects of nanohydroxyapatite (nHAp) particles on the morphological, chemical, physical, and biological properties of chitosan electrospun nanofibers. Materials and methods: nHAp particles with a 1.67 Ca/P ratio were synthesized via the aqueous precipitation method, incorporated into chitosan polymer solution (0.5 wt%), and electrospun into nHAp-loaded fibers (ChHa fibers). Neat chitosan fibers (nHAp-free, Ch fibers) were used as the control. The electrospun fiber mats were characterized using morphological, topographical, chemical, thermal, and a range of biological (antibacterial, antibiofilm, cell viability, and alkaline phosphatase [ALP] activity) analyses. Data were analyzed using ANOVA and Tukey’s test (α = 0.05). Results: ChHa fibers demonstrated a bead-like morphology, with thinner (331 ± 110 nm) and smoother (Ra = 2.9 ± 0.3 μm) distribution as compared to the control fibers. Despite showing similar cell viability and ALP activity to Ch fibers, the ChHa fibers demonstrated greater antibacterial potential against most tested bacteria (except for P. intermedia), and higher antibiofilm activity against P. gingivalis biofilm. Conclusions: The incorporation of nHAp particles did not jeopardize the overall morphology, topography, physical, and biological characteristics of the chitosan nanofibers. Clinical relevance: The combination of nHAp particles with chitosan can be used to engineer bioactive, electrospun composite nanofibers with potential applications in regenerative dentistry.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Department of Dental Materials and Prosthodontics Institute of Science and Technology (IST) São Paulo State University (UNESP), Av Engenheiro Francisco José Longo, 777, Jardim São DimasLaboratory of Biomedical Nanotechnology Universidade BrasilPlasma and Processes Laboratory Technological Institute of Aeronautics (ITA-CTA)Department of Oral Pathology and Microbiology IST UNESPDepartment of Conservative Dentistry School of Dentistry Federal University of Rio Grande do SulDepartment of Cariology Restorative Sciences and Endodontics University of Michigan School of Dentistry, 1011 N. University (Room 5223)Associated Laboratory of Materials and Sensor (LAS) National Institute of Space Research (INPE)Interdisciplinary Laboratory for Advanced Materials Federal University of PiauíDepartment of Dental Materials and Prosthodontics Institute of Science and Technology (IST) São Paulo State University (UNESP), Av Engenheiro Francisco José Longo, 777, Jardim São DimasDepartment of Oral Pathology and Microbiology IST UNESPFAPESP: 2011/17877-7FAPESP: 2011/20345-7CNPq: 310659/2014-0CNPq: 310973/2014-7Universidade Estadual Paulista (Unesp)Universidade BrasilTechnological Institute of Aeronautics (ITA-CTA)Federal University of Rio Grande do SulUniversity of Michigan School of DentistryNational Institute of Space Research (INPE)Federal University of PiauíSato, Tabata P. [UNESP]Rodrigues, Bruno V. M.Mello, Daphne C. R. [UNESP]Münchow, Eliseu A.Ribeiro, Juliana S.Machado, João Paulo B.Vasconcellos, Luana M. R. [UNESP]Lobo, Anderson O.Bottino, Marco C.Borges, Alexandre L. S. [UNESP]2021-06-25T10:13:03Z2021-06-25T10:13:03Z2021-05-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article3095-3103http://dx.doi.org/10.1007/s00784-020-03633-6Clinical Oral Investigations, v. 25, n. 5, p. 3095-3103, 2021.1436-37711432-6981http://hdl.handle.net/11449/20530010.1007/s00784-020-03633-62-s2.0-85092503065Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengClinical Oral Investigationsinfo:eu-repo/semantics/openAccess2021-10-23T12:31:23Zoai:repositorio.unesp.br:11449/205300Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T20:16:39.288506Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv The role of nanohydroxyapatite on the morphological, physical, and biological properties of chitosan nanofibers
title The role of nanohydroxyapatite on the morphological, physical, and biological properties of chitosan nanofibers
spellingShingle The role of nanohydroxyapatite on the morphological, physical, and biological properties of chitosan nanofibers
Sato, Tabata P. [UNESP]
Antimicrobial
Chitosan
Electrospinning
Regeneration
Regenerative dentistry
Scaffolds
title_short The role of nanohydroxyapatite on the morphological, physical, and biological properties of chitosan nanofibers
title_full The role of nanohydroxyapatite on the morphological, physical, and biological properties of chitosan nanofibers
title_fullStr The role of nanohydroxyapatite on the morphological, physical, and biological properties of chitosan nanofibers
title_full_unstemmed The role of nanohydroxyapatite on the morphological, physical, and biological properties of chitosan nanofibers
title_sort The role of nanohydroxyapatite on the morphological, physical, and biological properties of chitosan nanofibers
author Sato, Tabata P. [UNESP]
author_facet Sato, Tabata P. [UNESP]
Rodrigues, Bruno V. M.
Mello, Daphne C. R. [UNESP]
Münchow, Eliseu A.
Ribeiro, Juliana S.
Machado, João Paulo B.
Vasconcellos, Luana M. R. [UNESP]
Lobo, Anderson O.
Bottino, Marco C.
Borges, Alexandre L. S. [UNESP]
author_role author
author2 Rodrigues, Bruno V. M.
Mello, Daphne C. R. [UNESP]
Münchow, Eliseu A.
Ribeiro, Juliana S.
Machado, João Paulo B.
Vasconcellos, Luana M. R. [UNESP]
Lobo, Anderson O.
Bottino, Marco C.
Borges, Alexandre L. S. [UNESP]
author2_role author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade Estadual Paulista (Unesp)
Universidade Brasil
Technological Institute of Aeronautics (ITA-CTA)
Federal University of Rio Grande do Sul
University of Michigan School of Dentistry
National Institute of Space Research (INPE)
Federal University of Piauí
dc.contributor.author.fl_str_mv Sato, Tabata P. [UNESP]
Rodrigues, Bruno V. M.
Mello, Daphne C. R. [UNESP]
Münchow, Eliseu A.
Ribeiro, Juliana S.
Machado, João Paulo B.
Vasconcellos, Luana M. R. [UNESP]
Lobo, Anderson O.
Bottino, Marco C.
Borges, Alexandre L. S. [UNESP]
dc.subject.por.fl_str_mv Antimicrobial
Chitosan
Electrospinning
Regeneration
Regenerative dentistry
Scaffolds
topic Antimicrobial
Chitosan
Electrospinning
Regeneration
Regenerative dentistry
Scaffolds
description Objectives: This study aimed to evaluate the effects of nanohydroxyapatite (nHAp) particles on the morphological, chemical, physical, and biological properties of chitosan electrospun nanofibers. Materials and methods: nHAp particles with a 1.67 Ca/P ratio were synthesized via the aqueous precipitation method, incorporated into chitosan polymer solution (0.5 wt%), and electrospun into nHAp-loaded fibers (ChHa fibers). Neat chitosan fibers (nHAp-free, Ch fibers) were used as the control. The electrospun fiber mats were characterized using morphological, topographical, chemical, thermal, and a range of biological (antibacterial, antibiofilm, cell viability, and alkaline phosphatase [ALP] activity) analyses. Data were analyzed using ANOVA and Tukey’s test (α = 0.05). Results: ChHa fibers demonstrated a bead-like morphology, with thinner (331 ± 110 nm) and smoother (Ra = 2.9 ± 0.3 μm) distribution as compared to the control fibers. Despite showing similar cell viability and ALP activity to Ch fibers, the ChHa fibers demonstrated greater antibacterial potential against most tested bacteria (except for P. intermedia), and higher antibiofilm activity against P. gingivalis biofilm. Conclusions: The incorporation of nHAp particles did not jeopardize the overall morphology, topography, physical, and biological characteristics of the chitosan nanofibers. Clinical relevance: The combination of nHAp particles with chitosan can be used to engineer bioactive, electrospun composite nanofibers with potential applications in regenerative dentistry.
publishDate 2021
dc.date.none.fl_str_mv 2021-06-25T10:13:03Z
2021-06-25T10:13:03Z
2021-05-01
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1007/s00784-020-03633-6
Clinical Oral Investigations, v. 25, n. 5, p. 3095-3103, 2021.
1436-3771
1432-6981
http://hdl.handle.net/11449/205300
10.1007/s00784-020-03633-6
2-s2.0-85092503065
url http://dx.doi.org/10.1007/s00784-020-03633-6
http://hdl.handle.net/11449/205300
identifier_str_mv Clinical Oral Investigations, v. 25, n. 5, p. 3095-3103, 2021.
1436-3771
1432-6981
10.1007/s00784-020-03633-6
2-s2.0-85092503065
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Clinical Oral Investigations
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 3095-3103
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
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