The role of nanohydroxyapatite on the morphological, physical, and biological properties of chitosan nanofibers
Autor(a) principal: | |
---|---|
Data de Publicação: | 2021 |
Outros Autores: | , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1007/s00784-020-03633-6 http://hdl.handle.net/11449/205300 |
Resumo: | Objectives: This study aimed to evaluate the effects of nanohydroxyapatite (nHAp) particles on the morphological, chemical, physical, and biological properties of chitosan electrospun nanofibers. Materials and methods: nHAp particles with a 1.67 Ca/P ratio were synthesized via the aqueous precipitation method, incorporated into chitosan polymer solution (0.5 wt%), and electrospun into nHAp-loaded fibers (ChHa fibers). Neat chitosan fibers (nHAp-free, Ch fibers) were used as the control. The electrospun fiber mats were characterized using morphological, topographical, chemical, thermal, and a range of biological (antibacterial, antibiofilm, cell viability, and alkaline phosphatase [ALP] activity) analyses. Data were analyzed using ANOVA and Tukey’s test (α = 0.05). Results: ChHa fibers demonstrated a bead-like morphology, with thinner (331 ± 110 nm) and smoother (Ra = 2.9 ± 0.3 μm) distribution as compared to the control fibers. Despite showing similar cell viability and ALP activity to Ch fibers, the ChHa fibers demonstrated greater antibacterial potential against most tested bacteria (except for P. intermedia), and higher antibiofilm activity against P. gingivalis biofilm. Conclusions: The incorporation of nHAp particles did not jeopardize the overall morphology, topography, physical, and biological characteristics of the chitosan nanofibers. Clinical relevance: The combination of nHAp particles with chitosan can be used to engineer bioactive, electrospun composite nanofibers with potential applications in regenerative dentistry. |
id |
UNSP_b4f8d1879a8b35463efa2f580b786453 |
---|---|
oai_identifier_str |
oai:repositorio.unesp.br:11449/205300 |
network_acronym_str |
UNSP |
network_name_str |
Repositório Institucional da UNESP |
repository_id_str |
2946 |
spelling |
The role of nanohydroxyapatite on the morphological, physical, and biological properties of chitosan nanofibersAntimicrobialChitosanElectrospinningRegenerationRegenerative dentistryScaffoldsObjectives: This study aimed to evaluate the effects of nanohydroxyapatite (nHAp) particles on the morphological, chemical, physical, and biological properties of chitosan electrospun nanofibers. Materials and methods: nHAp particles with a 1.67 Ca/P ratio were synthesized via the aqueous precipitation method, incorporated into chitosan polymer solution (0.5 wt%), and electrospun into nHAp-loaded fibers (ChHa fibers). Neat chitosan fibers (nHAp-free, Ch fibers) were used as the control. The electrospun fiber mats were characterized using morphological, topographical, chemical, thermal, and a range of biological (antibacterial, antibiofilm, cell viability, and alkaline phosphatase [ALP] activity) analyses. Data were analyzed using ANOVA and Tukey’s test (α = 0.05). Results: ChHa fibers demonstrated a bead-like morphology, with thinner (331 ± 110 nm) and smoother (Ra = 2.9 ± 0.3 μm) distribution as compared to the control fibers. Despite showing similar cell viability and ALP activity to Ch fibers, the ChHa fibers demonstrated greater antibacterial potential against most tested bacteria (except for P. intermedia), and higher antibiofilm activity against P. gingivalis biofilm. Conclusions: The incorporation of nHAp particles did not jeopardize the overall morphology, topography, physical, and biological characteristics of the chitosan nanofibers. Clinical relevance: The combination of nHAp particles with chitosan can be used to engineer bioactive, electrospun composite nanofibers with potential applications in regenerative dentistry.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Department of Dental Materials and Prosthodontics Institute of Science and Technology (IST) São Paulo State University (UNESP), Av Engenheiro Francisco José Longo, 777, Jardim São DimasLaboratory of Biomedical Nanotechnology Universidade BrasilPlasma and Processes Laboratory Technological Institute of Aeronautics (ITA-CTA)Department of Oral Pathology and Microbiology IST UNESPDepartment of Conservative Dentistry School of Dentistry Federal University of Rio Grande do SulDepartment of Cariology Restorative Sciences and Endodontics University of Michigan School of Dentistry, 1011 N. University (Room 5223)Associated Laboratory of Materials and Sensor (LAS) National Institute of Space Research (INPE)Interdisciplinary Laboratory for Advanced Materials Federal University of PiauíDepartment of Dental Materials and Prosthodontics Institute of Science and Technology (IST) São Paulo State University (UNESP), Av Engenheiro Francisco José Longo, 777, Jardim São DimasDepartment of Oral Pathology and Microbiology IST UNESPFAPESP: 2011/17877-7FAPESP: 2011/20345-7CNPq: 310659/2014-0CNPq: 310973/2014-7Universidade Estadual Paulista (Unesp)Universidade BrasilTechnological Institute of Aeronautics (ITA-CTA)Federal University of Rio Grande do SulUniversity of Michigan School of DentistryNational Institute of Space Research (INPE)Federal University of PiauíSato, Tabata P. [UNESP]Rodrigues, Bruno V. M.Mello, Daphne C. R. [UNESP]Münchow, Eliseu A.Ribeiro, Juliana S.Machado, João Paulo B.Vasconcellos, Luana M. R. [UNESP]Lobo, Anderson O.Bottino, Marco C.Borges, Alexandre L. S. [UNESP]2021-06-25T10:13:03Z2021-06-25T10:13:03Z2021-05-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article3095-3103http://dx.doi.org/10.1007/s00784-020-03633-6Clinical Oral Investigations, v. 25, n. 5, p. 3095-3103, 2021.1436-37711432-6981http://hdl.handle.net/11449/20530010.1007/s00784-020-03633-62-s2.0-85092503065Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengClinical Oral Investigationsinfo:eu-repo/semantics/openAccess2021-10-23T12:31:23Zoai:repositorio.unesp.br:11449/205300Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T20:16:39.288506Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
The role of nanohydroxyapatite on the morphological, physical, and biological properties of chitosan nanofibers |
title |
The role of nanohydroxyapatite on the morphological, physical, and biological properties of chitosan nanofibers |
spellingShingle |
The role of nanohydroxyapatite on the morphological, physical, and biological properties of chitosan nanofibers Sato, Tabata P. [UNESP] Antimicrobial Chitosan Electrospinning Regeneration Regenerative dentistry Scaffolds |
title_short |
The role of nanohydroxyapatite on the morphological, physical, and biological properties of chitosan nanofibers |
title_full |
The role of nanohydroxyapatite on the morphological, physical, and biological properties of chitosan nanofibers |
title_fullStr |
The role of nanohydroxyapatite on the morphological, physical, and biological properties of chitosan nanofibers |
title_full_unstemmed |
The role of nanohydroxyapatite on the morphological, physical, and biological properties of chitosan nanofibers |
title_sort |
The role of nanohydroxyapatite on the morphological, physical, and biological properties of chitosan nanofibers |
author |
Sato, Tabata P. [UNESP] |
author_facet |
Sato, Tabata P. [UNESP] Rodrigues, Bruno V. M. Mello, Daphne C. R. [UNESP] Münchow, Eliseu A. Ribeiro, Juliana S. Machado, João Paulo B. Vasconcellos, Luana M. R. [UNESP] Lobo, Anderson O. Bottino, Marco C. Borges, Alexandre L. S. [UNESP] |
author_role |
author |
author2 |
Rodrigues, Bruno V. M. Mello, Daphne C. R. [UNESP] Münchow, Eliseu A. Ribeiro, Juliana S. Machado, João Paulo B. Vasconcellos, Luana M. R. [UNESP] Lobo, Anderson O. Bottino, Marco C. Borges, Alexandre L. S. [UNESP] |
author2_role |
author author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) Universidade Brasil Technological Institute of Aeronautics (ITA-CTA) Federal University of Rio Grande do Sul University of Michigan School of Dentistry National Institute of Space Research (INPE) Federal University of Piauí |
dc.contributor.author.fl_str_mv |
Sato, Tabata P. [UNESP] Rodrigues, Bruno V. M. Mello, Daphne C. R. [UNESP] Münchow, Eliseu A. Ribeiro, Juliana S. Machado, João Paulo B. Vasconcellos, Luana M. R. [UNESP] Lobo, Anderson O. Bottino, Marco C. Borges, Alexandre L. S. [UNESP] |
dc.subject.por.fl_str_mv |
Antimicrobial Chitosan Electrospinning Regeneration Regenerative dentistry Scaffolds |
topic |
Antimicrobial Chitosan Electrospinning Regeneration Regenerative dentistry Scaffolds |
description |
Objectives: This study aimed to evaluate the effects of nanohydroxyapatite (nHAp) particles on the morphological, chemical, physical, and biological properties of chitosan electrospun nanofibers. Materials and methods: nHAp particles with a 1.67 Ca/P ratio were synthesized via the aqueous precipitation method, incorporated into chitosan polymer solution (0.5 wt%), and electrospun into nHAp-loaded fibers (ChHa fibers). Neat chitosan fibers (nHAp-free, Ch fibers) were used as the control. The electrospun fiber mats were characterized using morphological, topographical, chemical, thermal, and a range of biological (antibacterial, antibiofilm, cell viability, and alkaline phosphatase [ALP] activity) analyses. Data were analyzed using ANOVA and Tukey’s test (α = 0.05). Results: ChHa fibers demonstrated a bead-like morphology, with thinner (331 ± 110 nm) and smoother (Ra = 2.9 ± 0.3 μm) distribution as compared to the control fibers. Despite showing similar cell viability and ALP activity to Ch fibers, the ChHa fibers demonstrated greater antibacterial potential against most tested bacteria (except for P. intermedia), and higher antibiofilm activity against P. gingivalis biofilm. Conclusions: The incorporation of nHAp particles did not jeopardize the overall morphology, topography, physical, and biological characteristics of the chitosan nanofibers. Clinical relevance: The combination of nHAp particles with chitosan can be used to engineer bioactive, electrospun composite nanofibers with potential applications in regenerative dentistry. |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021-06-25T10:13:03Z 2021-06-25T10:13:03Z 2021-05-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1007/s00784-020-03633-6 Clinical Oral Investigations, v. 25, n. 5, p. 3095-3103, 2021. 1436-3771 1432-6981 http://hdl.handle.net/11449/205300 10.1007/s00784-020-03633-6 2-s2.0-85092503065 |
url |
http://dx.doi.org/10.1007/s00784-020-03633-6 http://hdl.handle.net/11449/205300 |
identifier_str_mv |
Clinical Oral Investigations, v. 25, n. 5, p. 3095-3103, 2021. 1436-3771 1432-6981 10.1007/s00784-020-03633-6 2-s2.0-85092503065 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Clinical Oral Investigations |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
3095-3103 |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808129182487543808 |