Description of electrophoretic and chromatographic hemoglobin profile of Rhinoclemmys punctularia
Autor(a) principal: | |
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Data de Publicação: | 2007 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://www.geneticsmr.com//year2007/vol6-2/pdf/gmr0301.pdf http://hdl.handle.net/11449/69876 |
Resumo: | Studies of the hemoglobin pattern in Brazilian reptiles are important for determining ecological and phylogenetic relationships, but they are scarce. Peripheral blood samples were obtained from 7 males and 18 females of Rhinoclemmys punctularia. The hematological profile was based on the total hemoglobin and hematocrit values. The hemoglobin profile was obtained using electrophoretic procedures at different pH, isoelectric focusing, globin chain electrophoresis, and HPLC. The hematocrit (31 ± 2%) and total hemoglobin (7.5 ± 0.2 g/dL) values did not indicate gender variations. Alkaline pH electrophoresis of the total blood samples treated with 1% saponin demonstrated the presence of four well-defined hemoglobin fractions, one major component (fraction I), showing cathodic migration and three others faster than fraction I with anodic migration. When the samples were precipitated with chloroform, only two hemoglobin fractions were observed, similar to fractions I and III from the first procedure. Isoelectric focusing and HPLC showed the same pattern. With acid and neutral pH electrophoresis, two fractions with anodic migration were observed. The globin chain identification at alkaline pH showed two fractions, but four fractions were observed at acidic pH, suggesting that different polypeptide chains are involved in the hemoglobin molecule. The chromatographic separation of the total blood sample demonstrated that the major fraction comprised 81.9% and the minor 18.1%. The results obtained demonstrated a similarity between these hemoglobin components and those of some Chelidae reported in the literature for both land and aquatic animals, reflecting the adaptation to environmental conditions. ©FUNPEC-RP. |
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Description of electrophoretic and chromatographic hemoglobin profile of Rhinoclemmys punctulariaElectrophoretic profileHemoglobin polymorphismHemoglobinsRhinoclemmys punctulariachloroformhemoglobinalkalinitycontrolled studyelectrophoresisfemalehematocrithemoglobin determinationhigh performance liquid chromatographyisoelectric focusingmalenonhumanpHpH measurementsex differenceturtleAnimalsChromatographyEcologyElectrophoresisFemaleHematocritHumansMaleModels, GeneticPhylogenyPolymorphism, GeneticReptilesTurtlesAnimaliaChelidaeReptiliaStudies of the hemoglobin pattern in Brazilian reptiles are important for determining ecological and phylogenetic relationships, but they are scarce. Peripheral blood samples were obtained from 7 males and 18 females of Rhinoclemmys punctularia. The hematological profile was based on the total hemoglobin and hematocrit values. The hemoglobin profile was obtained using electrophoretic procedures at different pH, isoelectric focusing, globin chain electrophoresis, and HPLC. The hematocrit (31 ± 2%) and total hemoglobin (7.5 ± 0.2 g/dL) values did not indicate gender variations. Alkaline pH electrophoresis of the total blood samples treated with 1% saponin demonstrated the presence of four well-defined hemoglobin fractions, one major component (fraction I), showing cathodic migration and three others faster than fraction I with anodic migration. When the samples were precipitated with chloroform, only two hemoglobin fractions were observed, similar to fractions I and III from the first procedure. Isoelectric focusing and HPLC showed the same pattern. With acid and neutral pH electrophoresis, two fractions with anodic migration were observed. The globin chain identification at alkaline pH showed two fractions, but four fractions were observed at acidic pH, suggesting that different polypeptide chains are involved in the hemoglobin molecule. The chromatographic separation of the total blood sample demonstrated that the major fraction comprised 81.9% and the minor 18.1%. The results obtained demonstrated a similarity between these hemoglobin components and those of some Chelidae reported in the literature for both land and aquatic animals, reflecting the adaptation to environmental conditions. ©FUNPEC-RP.Laboratório de Hemoglobinas e Genética Das Doenças Hematológicas Departamento de Biologia IBILCE, S. José do Rio Preto, SPMuseu Paraense Emilio Goeldi, Belém, PALaboratório de Dermatoimunologia UEPA/UFPA/MC, Belém, PALaboratório de Hemoglobinas e Genética Das Doenças Hematológicas Departamento de Biologia IBILCE, S. José do Rio Preto, SPUniversidade Estadual Paulista (Unesp)Museu Paraense Emilio GoeldiUniversidade Federal do Pará (UFPA)Bonini-Domingos, C. R. [UNESP]Silva, M. B. [UNESP]Romero, R. M. [UNESP]Zamaro, P. J A [UNESP]Ondei, L. S. [UNESP]Zago, C. E S [UNESP]Moreira, S. B.Salgado, C. G.2014-05-27T11:22:35Z2014-05-27T11:22:35Z2007-09-03info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article415-421application/pdfhttp://www.geneticsmr.com//year2007/vol6-2/pdf/gmr0301.pdfGenetics and Molecular Research, v. 6, n. 2, p. 415-421, 2007.1676-5680http://hdl.handle.net/11449/69876WOS:0002516964000192-s2.0-345482822272-s2.0-34548282227.pdf32794280661767190000-0002-4603-9467Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengGenetics and Molecular Research0,439info:eu-repo/semantics/openAccess2023-12-29T06:17:00Zoai:repositorio.unesp.br:11449/69876Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T21:34:18.678853Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Description of electrophoretic and chromatographic hemoglobin profile of Rhinoclemmys punctularia |
title |
Description of electrophoretic and chromatographic hemoglobin profile of Rhinoclemmys punctularia |
spellingShingle |
Description of electrophoretic and chromatographic hemoglobin profile of Rhinoclemmys punctularia Bonini-Domingos, C. R. [UNESP] Electrophoretic profile Hemoglobin polymorphism Hemoglobins Rhinoclemmys punctularia chloroform hemoglobin alkalinity controlled study electrophoresis female hematocrit hemoglobin determination high performance liquid chromatography isoelectric focusing male nonhuman pH pH measurement sex difference turtle Animals Chromatography Ecology Electrophoresis Female Hematocrit Humans Male Models, Genetic Phylogeny Polymorphism, Genetic Reptiles Turtles Animalia Chelidae Reptilia |
title_short |
Description of electrophoretic and chromatographic hemoglobin profile of Rhinoclemmys punctularia |
title_full |
Description of electrophoretic and chromatographic hemoglobin profile of Rhinoclemmys punctularia |
title_fullStr |
Description of electrophoretic and chromatographic hemoglobin profile of Rhinoclemmys punctularia |
title_full_unstemmed |
Description of electrophoretic and chromatographic hemoglobin profile of Rhinoclemmys punctularia |
title_sort |
Description of electrophoretic and chromatographic hemoglobin profile of Rhinoclemmys punctularia |
author |
Bonini-Domingos, C. R. [UNESP] |
author_facet |
Bonini-Domingos, C. R. [UNESP] Silva, M. B. [UNESP] Romero, R. M. [UNESP] Zamaro, P. J A [UNESP] Ondei, L. S. [UNESP] Zago, C. E S [UNESP] Moreira, S. B. Salgado, C. G. |
author_role |
author |
author2 |
Silva, M. B. [UNESP] Romero, R. M. [UNESP] Zamaro, P. J A [UNESP] Ondei, L. S. [UNESP] Zago, C. E S [UNESP] Moreira, S. B. Salgado, C. G. |
author2_role |
author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) Museu Paraense Emilio Goeldi Universidade Federal do Pará (UFPA) |
dc.contributor.author.fl_str_mv |
Bonini-Domingos, C. R. [UNESP] Silva, M. B. [UNESP] Romero, R. M. [UNESP] Zamaro, P. J A [UNESP] Ondei, L. S. [UNESP] Zago, C. E S [UNESP] Moreira, S. B. Salgado, C. G. |
dc.subject.por.fl_str_mv |
Electrophoretic profile Hemoglobin polymorphism Hemoglobins Rhinoclemmys punctularia chloroform hemoglobin alkalinity controlled study electrophoresis female hematocrit hemoglobin determination high performance liquid chromatography isoelectric focusing male nonhuman pH pH measurement sex difference turtle Animals Chromatography Ecology Electrophoresis Female Hematocrit Humans Male Models, Genetic Phylogeny Polymorphism, Genetic Reptiles Turtles Animalia Chelidae Reptilia |
topic |
Electrophoretic profile Hemoglobin polymorphism Hemoglobins Rhinoclemmys punctularia chloroform hemoglobin alkalinity controlled study electrophoresis female hematocrit hemoglobin determination high performance liquid chromatography isoelectric focusing male nonhuman pH pH measurement sex difference turtle Animals Chromatography Ecology Electrophoresis Female Hematocrit Humans Male Models, Genetic Phylogeny Polymorphism, Genetic Reptiles Turtles Animalia Chelidae Reptilia |
description |
Studies of the hemoglobin pattern in Brazilian reptiles are important for determining ecological and phylogenetic relationships, but they are scarce. Peripheral blood samples were obtained from 7 males and 18 females of Rhinoclemmys punctularia. The hematological profile was based on the total hemoglobin and hematocrit values. The hemoglobin profile was obtained using electrophoretic procedures at different pH, isoelectric focusing, globin chain electrophoresis, and HPLC. The hematocrit (31 ± 2%) and total hemoglobin (7.5 ± 0.2 g/dL) values did not indicate gender variations. Alkaline pH electrophoresis of the total blood samples treated with 1% saponin demonstrated the presence of four well-defined hemoglobin fractions, one major component (fraction I), showing cathodic migration and three others faster than fraction I with anodic migration. When the samples were precipitated with chloroform, only two hemoglobin fractions were observed, similar to fractions I and III from the first procedure. Isoelectric focusing and HPLC showed the same pattern. With acid and neutral pH electrophoresis, two fractions with anodic migration were observed. The globin chain identification at alkaline pH showed two fractions, but four fractions were observed at acidic pH, suggesting that different polypeptide chains are involved in the hemoglobin molecule. The chromatographic separation of the total blood sample demonstrated that the major fraction comprised 81.9% and the minor 18.1%. The results obtained demonstrated a similarity between these hemoglobin components and those of some Chelidae reported in the literature for both land and aquatic animals, reflecting the adaptation to environmental conditions. ©FUNPEC-RP. |
publishDate |
2007 |
dc.date.none.fl_str_mv |
2007-09-03 2014-05-27T11:22:35Z 2014-05-27T11:22:35Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://www.geneticsmr.com//year2007/vol6-2/pdf/gmr0301.pdf Genetics and Molecular Research, v. 6, n. 2, p. 415-421, 2007. 1676-5680 http://hdl.handle.net/11449/69876 WOS:000251696400019 2-s2.0-34548282227 2-s2.0-34548282227.pdf 3279428066176719 0000-0002-4603-9467 |
url |
http://www.geneticsmr.com//year2007/vol6-2/pdf/gmr0301.pdf http://hdl.handle.net/11449/69876 |
identifier_str_mv |
Genetics and Molecular Research, v. 6, n. 2, p. 415-421, 2007. 1676-5680 WOS:000251696400019 2-s2.0-34548282227 2-s2.0-34548282227.pdf 3279428066176719 0000-0002-4603-9467 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Genetics and Molecular Research 0,439 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
415-421 application/pdf |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
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1808129337101123584 |