Ação antimicrobiana de compostos majoritários presentes em óleos essenciais sobre bactérias causadoras de infecções hospitalares

• Autor(a) principal: Leal, Priscila Martins [UNESP]
• Data de Publicação: 2013
• Tipo de documento: Trabalho de conclusão de curso
• Idioma: por
• Título da fonte: Repositório Institucional da UNESP
• Texto Completo: http://hdl.handle.net/11449/124163; http://www.athena.biblioteca.unesp.br/exlibris/bd/capelo/2015-06-15/000830139.pdf
• topic: Infeccão hospitalar; Essencias e oleos essenciais - Uso terapeutico; Plantas medicinais; Agentes anti-infecciosos; Essences and essential oils - Therapeutic use

Nosocomial diseases are frequently associated with hospitalized patients or people exposed to invasive procedures at hospitals. The etiologic agents of these diseases are commonly represented by resistant or multi resistant bacteria samples. This way, the search for new antimicrobial compounds is important, including essential oils or extracts of medicinal plants and its major compounds. These compounds are often responsable for the biological and antimicrobial properties of the essential oils. The aim of this study was to evaluate the inhibitory effects of six major compounds (carvacrol, eugenol, geraniol, terpineol and thymol) from different plants over the nosocomial bacterias: Staphylococcus aureus Meticilin Resistant (MRSA), Staphylococcus aureus Meticilin Sensible (MSSA), Pseudomonas aeruginosa, Enterococcus faecalis and Escherichia coli. Therefore it was determined determine the Minimal Inhibitory Concentration (MIC) by using the diluition method of antimicrobial compounds in Mueller-Hinton Agar (MHA). According to the results obtained by these sensitivity tests, the tested compounds exhibit antimicrobial activity against those bacterias. The inhibition character found was higher in S. aureus samples and lower in P. aeruginosa strains. In order to determine if the MIC values obtained for these compounds refers to bactericidal or bacteriostatic actions, assays were then performed to obtain the survival curves of each bacteria under each antimicrobial compound action, by counting viable colonies over time. Thus, to E. faecalis, geraniol was the most effective compound; For E. coli, it was carvacrol; For MRSA and MSSA, thymol was the most effective one. And for P. aeruginosa, carvacrol, eugenol and thymol were the most effective antimicrobial compounds. These results allows to conclude that the major compounds tested has antimicrobial properties and presents bactericidal or bacteriostatic actions, depending on the bacteria and the compound ...