Study protocol to investigate biomolecular muscle profile as predictors of long-term urinary incontinence in women with gestational diabetes mellitus
Autor(a) principal: | |
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Data de Publicação: | 2020 |
Outros Autores: | , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1186/s12884-020-2749-x http://hdl.handle.net/11449/200135 |
Resumo: | Background: Pelvic floor muscles (PFM) and rectus abdominis muscles (RAM) of pregnant diabetic rats exhibit atrophy, co-localization of fast and slow fibers and an increased collagen type I/III ratio. However, the role of similar PFM or RAM hyperglycemic-related myopathy in women with gestational diabetes mellitus (GDM) remains poorly investigated. This study aims to assess the frequency of pelvic floor muscle disorders and pregnancy-specific urinary incontinence (PS-UI) 12 months after the Cesarean (C) section in women with GDM. Specifically, differences in PFM/RAM hyperglycemic myopathy will be evaluated. Methods: The Diamater is an ongoing cohort study of four groups of 59 pregnant women each from the Perinatal Diabetes Research Centre (PDRC), Botucatu Medical School (FMB)-UNESP (São Paulo State University), Brazil. Diagnosis of GDM and PS-UI will be made at 24-26 weeks, with a follow-up at 34-38 weeks of gestation. Inclusion in the study will occur at the time of C-section, and patients will be followed at 24-48 h, 6 weeks and 6 and 12 months postpartum. Study groups will be classified as (1) GDM plus PS-UI; (2) GDM without PS-UI; (3) Non-GDM plus PS-UI; and (4) Non-GDM without PS-UI. We will analyze relationships between GDM, PS-UI and hyperglycemic myopathy at 12 months after C-section. The mediator variables to be evaluated include digital palpation, vaginal squeeze pressure, 3D pelvic floor ultrasound, and 3D RAM ultrasound. RAM samples obtained during C-section will be analyzed for ex-vivo contractility, morphological, molecular and OMICS profiles to further characterize the hyperglycemic myopathy. Additional variables to be evaluated include maternal age, socioeconomic status, educational level, ethnicity, body mass index, weight gain during pregnancy, quality of glycemic control and insulin therapy. Discussion: To our knowledge, this will be the first study to provide data on the prevalence of PS-UI and RAM and PFM physical and biomolecular muscle profiles after C-section in mothers with GDM. The longitudinal design allows for the assessment of cause-effect relationships between GDM, PS-UI, and PFMs and RAMs myopathy. The findings may reveal previously undetermined consequences of GDM. |
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Study protocol to investigate biomolecular muscle profile as predictors of long-term urinary incontinence in women with gestational diabetes mellitusCollagenElectromyographyGestational diabetes mellitusHyperglycemic myopathyPelvic floor musclesProteomicsRectus abdominis musclesTransmission electron microscopyUrinary incontinenceBackground: Pelvic floor muscles (PFM) and rectus abdominis muscles (RAM) of pregnant diabetic rats exhibit atrophy, co-localization of fast and slow fibers and an increased collagen type I/III ratio. However, the role of similar PFM or RAM hyperglycemic-related myopathy in women with gestational diabetes mellitus (GDM) remains poorly investigated. This study aims to assess the frequency of pelvic floor muscle disorders and pregnancy-specific urinary incontinence (PS-UI) 12 months after the Cesarean (C) section in women with GDM. Specifically, differences in PFM/RAM hyperglycemic myopathy will be evaluated. Methods: The Diamater is an ongoing cohort study of four groups of 59 pregnant women each from the Perinatal Diabetes Research Centre (PDRC), Botucatu Medical School (FMB)-UNESP (São Paulo State University), Brazil. Diagnosis of GDM and PS-UI will be made at 24-26 weeks, with a follow-up at 34-38 weeks of gestation. Inclusion in the study will occur at the time of C-section, and patients will be followed at 24-48 h, 6 weeks and 6 and 12 months postpartum. Study groups will be classified as (1) GDM plus PS-UI; (2) GDM without PS-UI; (3) Non-GDM plus PS-UI; and (4) Non-GDM without PS-UI. We will analyze relationships between GDM, PS-UI and hyperglycemic myopathy at 12 months after C-section. The mediator variables to be evaluated include digital palpation, vaginal squeeze pressure, 3D pelvic floor ultrasound, and 3D RAM ultrasound. RAM samples obtained during C-section will be analyzed for ex-vivo contractility, morphological, molecular and OMICS profiles to further characterize the hyperglycemic myopathy. Additional variables to be evaluated include maternal age, socioeconomic status, educational level, ethnicity, body mass index, weight gain during pregnancy, quality of glycemic control and insulin therapy. Discussion: To our knowledge, this will be the first study to provide data on the prevalence of PS-UI and RAM and PFM physical and biomolecular muscle profiles after C-section in mothers with GDM. The longitudinal design allows for the assessment of cause-effect relationships between GDM, PS-UI, and PFMs and RAMs myopathy. The findings may reveal previously undetermined consequences of GDM.Department of Gynecology and Obstetrics Botucatu Medical School (FMB) São Paulo State University (UNESP)Physics Department Institute of Biosciences Letters and Exact Sciences Multiuser Center for Biomolecular Innovation UNESP-São Paulo State UniversityPhysiotherapy Department Faculdades Integradas de Bauru FIBUniversidade Do Sagrado Coração (USC), Jardim BrasilDepartment of Health Research Methods Evidence and Impact McMaster UniversityBiostatistics Unit Father Sean o'Sullivan Research Centre St Joseph's Healthcare-HamiltonDepartment of Obstetrics and Gynecology Weill Cornell MedicineInstitute of Tropical Medicine University of Sao Paulo Medical SchoolSchool of Philosophy and Sciences Department of Physiotherapy and Occupational Therapy UNESP-São Paulo State UniversityDepartment of Gynecology and Obstetrics Botucatu Medical School (FMB) São Paulo State University (UNESP)Physics Department Institute of Biosciences Letters and Exact Sciences Multiuser Center for Biomolecular Innovation UNESP-São Paulo State UniversitySchool of Philosophy and Sciences Department of Physiotherapy and Occupational Therapy UNESP-São Paulo State UniversityUniversidade Estadual Paulista (Unesp)FIBUniversidade Do Sagrado Coração (USC)McMaster UniversitySt Joseph's Healthcare-HamiltonWeill Cornell MedicineUniversidade de São Paulo (USP)Rudge, Marilza V. C. [UNESP]Souza, Fátima P. [UNESP]Abbade, Joelcio F. [UNESP]Hallur, Raghavendra L. S. [UNESP]Marcondes, João Paulo C. [UNESP]Piculo, Fernanda [UNESP]Marini, Gabriela [UNESP]Vesentini, Giovana [UNESP]Thabane, LehanaWitkin, Steven S.Calderon, Iracema M. P. [UNESP]Barbosa, Angélica M. P. [UNESP]Barbosa, A. M.P.Calderon, I. M.P.Souza, F. P.Abbade, J. F.Hallur, L. S.R.Piculo, F.Marini, G.Thabane, L.Palma, M. S.Graeff, C. F.O.Arni, R. K.Herculano, R. D.Salvadori, D. F.Mateus, S.Dal Pai Silva, M.Magalhães, C. G.Costa, R. A.Lima, S. A.M.Felisbino, S. L.Barbosa, W.Atallah, A.Girão, M. J.B.Di Bella, Z.Uchoa, S. M.Payão, S.Hijas, A.Berghman, B.De Bie, R.Sobrevia, L.Junginger, B.Alves, F. C.B.Rossignoli, P. S.Prudencio, C. B.Orlandi, M. I.G.Gonçalves, M. I.Nunes, S. K.Catinelli, B. B.Quiroz, S.Sarmento, B. V.Pinheiro, F. A.Sartorão, C. I.Lucas, R. R.Reyes, D. R.A.Quiroz, S. B.C.V.Enriquez, E. M.A.Oliveira, R. G.Floriano, J. F.Barneze, S.Dangió, T. D.Pascon, T.Rossignoli, P.Freitas, J. V.Takano, L.Reis, F.Caldeirão, T. D.Fernandes, J. N.Carr, A. M.Gaitero, M. V.C.Corrente, J. E.Nunes, H. R.C.Candido, A. F.Costa, S. M.B.Melo, J. V.F.Reis, F. V.D.S.Garcia, G. A.Rabadan, G. B.Bassin, H. C.M.Suyama, K. S.Damasceno, L. N.Takemoto, M. L.S.Menezes, M. D.Bussaneli, D. G.Nogueira, V. K.C.Lima, P. R.Lourenço, I. O.Marostica De Sá, J.Megid, R. A.Caruso, I. P.Rasmussen, L. T.Prata, G. M.Arantes, M. A.Ferraz, G. A.R.Camargo, L. P.Kron, M. R.2020-12-12T01:58:39Z2020-12-12T01:58:39Z2020-02-19info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.1186/s12884-020-2749-xBMC Pregnancy and Childbirth, v. 20, n. 1, 2020.1471-2393http://hdl.handle.net/11449/20013510.1186/s12884-020-2749-x2-s2.0-8508114437491625089789458870000-0003-2460-1145Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengBMC Pregnancy and Childbirthinfo:eu-repo/semantics/openAccess2024-08-16T14:06:43Zoai:repositorio.unesp.br:11449/200135Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-16T14:06:43Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Study protocol to investigate biomolecular muscle profile as predictors of long-term urinary incontinence in women with gestational diabetes mellitus |
title |
Study protocol to investigate biomolecular muscle profile as predictors of long-term urinary incontinence in women with gestational diabetes mellitus |
spellingShingle |
Study protocol to investigate biomolecular muscle profile as predictors of long-term urinary incontinence in women with gestational diabetes mellitus Rudge, Marilza V. C. [UNESP] Collagen Electromyography Gestational diabetes mellitus Hyperglycemic myopathy Pelvic floor muscles Proteomics Rectus abdominis muscles Transmission electron microscopy Urinary incontinence |
title_short |
Study protocol to investigate biomolecular muscle profile as predictors of long-term urinary incontinence in women with gestational diabetes mellitus |
title_full |
Study protocol to investigate biomolecular muscle profile as predictors of long-term urinary incontinence in women with gestational diabetes mellitus |
title_fullStr |
Study protocol to investigate biomolecular muscle profile as predictors of long-term urinary incontinence in women with gestational diabetes mellitus |
title_full_unstemmed |
Study protocol to investigate biomolecular muscle profile as predictors of long-term urinary incontinence in women with gestational diabetes mellitus |
title_sort |
Study protocol to investigate biomolecular muscle profile as predictors of long-term urinary incontinence in women with gestational diabetes mellitus |
author |
Rudge, Marilza V. C. [UNESP] |
author_facet |
Rudge, Marilza V. C. [UNESP] Souza, Fátima P. [UNESP] Abbade, Joelcio F. [UNESP] Hallur, Raghavendra L. S. [UNESP] Marcondes, João Paulo C. [UNESP] Piculo, Fernanda [UNESP] Marini, Gabriela [UNESP] Vesentini, Giovana [UNESP] Thabane, Lehana Witkin, Steven S. Calderon, Iracema M. P. [UNESP] Barbosa, Angélica M. P. [UNESP] Barbosa, A. M.P. Calderon, I. M.P. Souza, F. P. Abbade, J. F. Hallur, L. S.R. Piculo, F. Marini, G. Thabane, L. Palma, M. S. Graeff, C. F.O. Arni, R. K. Herculano, R. D. Salvadori, D. F. Mateus, S. Dal Pai Silva, M. Magalhães, C. G. Costa, R. A. Lima, S. A.M. Felisbino, S. L. Barbosa, W. Atallah, A. Girão, M. J.B. Di Bella, Z. Uchoa, S. M. Payão, S. Hijas, A. Berghman, B. De Bie, R. Sobrevia, L. Junginger, B. Alves, F. C.B. Rossignoli, P. S. Prudencio, C. B. Orlandi, M. I.G. Gonçalves, M. I. Nunes, S. K. Catinelli, B. B. Quiroz, S. Sarmento, B. V. Pinheiro, F. A. Sartorão, C. I. Lucas, R. R. Reyes, D. R.A. Quiroz, S. B.C.V. Enriquez, E. M.A. Oliveira, R. G. Floriano, J. F. Barneze, S. Dangió, T. D. Pascon, T. Rossignoli, P. Freitas, J. V. Takano, L. Reis, F. Caldeirão, T. D. Fernandes, J. N. Carr, A. M. Gaitero, M. V.C. Corrente, J. E. Nunes, H. R.C. Candido, A. F. Costa, S. M.B. Melo, J. V.F. Reis, F. V.D.S. Garcia, G. A. Rabadan, G. B. Bassin, H. C.M. Suyama, K. S. Damasceno, L. N. Takemoto, M. L.S. Menezes, M. D. Bussaneli, D. G. Nogueira, V. K.C. Lima, P. R. Lourenço, I. O. Marostica De Sá, J. Megid, R. A. Caruso, I. P. Rasmussen, L. T. Prata, G. M. Arantes, M. A. Ferraz, G. A.R. Camargo, L. P. Kron, M. R. |
author_role |
author |
author2 |
Souza, Fátima P. [UNESP] Abbade, Joelcio F. [UNESP] Hallur, Raghavendra L. S. [UNESP] Marcondes, João Paulo C. [UNESP] Piculo, Fernanda [UNESP] Marini, Gabriela [UNESP] Vesentini, Giovana [UNESP] Thabane, Lehana Witkin, Steven S. Calderon, Iracema M. P. [UNESP] Barbosa, Angélica M. P. [UNESP] Barbosa, A. M.P. Calderon, I. M.P. Souza, F. P. Abbade, J. F. Hallur, L. S.R. Piculo, F. Marini, G. Thabane, L. Palma, M. S. Graeff, C. F.O. Arni, R. K. Herculano, R. D. Salvadori, D. F. Mateus, S. Dal Pai Silva, M. Magalhães, C. G. Costa, R. A. Lima, S. A.M. Felisbino, S. L. Barbosa, W. Atallah, A. Girão, M. J.B. Di Bella, Z. Uchoa, S. M. Payão, S. Hijas, A. Berghman, B. De Bie, R. Sobrevia, L. Junginger, B. Alves, F. C.B. Rossignoli, P. S. Prudencio, C. B. Orlandi, M. I.G. Gonçalves, M. I. Nunes, S. K. Catinelli, B. B. Quiroz, S. Sarmento, B. V. Pinheiro, F. A. Sartorão, C. I. Lucas, R. R. Reyes, D. R.A. Quiroz, S. B.C.V. Enriquez, E. M.A. Oliveira, R. G. Floriano, J. F. Barneze, S. Dangió, T. D. Pascon, T. Rossignoli, P. Freitas, J. V. Takano, L. Reis, F. Caldeirão, T. D. Fernandes, J. N. Carr, A. M. Gaitero, M. V.C. Corrente, J. E. Nunes, H. R.C. Candido, A. F. Costa, S. M.B. Melo, J. V.F. Reis, F. V.D.S. Garcia, G. A. Rabadan, G. B. Bassin, H. C.M. Suyama, K. S. Damasceno, L. N. Takemoto, M. L.S. Menezes, M. D. Bussaneli, D. G. Nogueira, V. K.C. Lima, P. R. Lourenço, I. O. Marostica De Sá, J. Megid, R. A. Caruso, I. P. Rasmussen, L. T. Prata, G. M. Arantes, M. A. Ferraz, G. A.R. Camargo, L. P. Kron, M. R. |
author2_role |
author author author author author author author author author author author author author author author author author author author author author author author author author author author author author author author author author author author author author author author author author author author author author author author author author author author author author author author author author author author author author author author author author author author author author author author author author author author author author author author author author author author author author author author author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) FIB Universidade Do Sagrado Coração (USC) McMaster University St Joseph's Healthcare-Hamilton Weill Cornell Medicine Universidade de São Paulo (USP) |
dc.contributor.author.fl_str_mv |
Rudge, Marilza V. C. [UNESP] Souza, Fátima P. [UNESP] Abbade, Joelcio F. [UNESP] Hallur, Raghavendra L. S. [UNESP] Marcondes, João Paulo C. [UNESP] Piculo, Fernanda [UNESP] Marini, Gabriela [UNESP] Vesentini, Giovana [UNESP] Thabane, Lehana Witkin, Steven S. Calderon, Iracema M. P. [UNESP] Barbosa, Angélica M. P. [UNESP] Barbosa, A. M.P. Calderon, I. M.P. Souza, F. P. Abbade, J. F. Hallur, L. S.R. Piculo, F. Marini, G. Thabane, L. Palma, M. S. Graeff, C. F.O. Arni, R. K. Herculano, R. D. Salvadori, D. F. Mateus, S. Dal Pai Silva, M. Magalhães, C. G. Costa, R. A. Lima, S. A.M. Felisbino, S. L. Barbosa, W. Atallah, A. Girão, M. J.B. Di Bella, Z. Uchoa, S. M. Payão, S. Hijas, A. Berghman, B. De Bie, R. Sobrevia, L. Junginger, B. Alves, F. C.B. Rossignoli, P. S. Prudencio, C. B. Orlandi, M. I.G. Gonçalves, M. I. Nunes, S. K. Catinelli, B. B. Quiroz, S. Sarmento, B. V. Pinheiro, F. A. Sartorão, C. I. Lucas, R. R. Reyes, D. R.A. Quiroz, S. B.C.V. Enriquez, E. M.A. Oliveira, R. G. Floriano, J. F. Barneze, S. Dangió, T. D. Pascon, T. Rossignoli, P. Freitas, J. V. Takano, L. Reis, F. Caldeirão, T. D. Fernandes, J. N. Carr, A. M. Gaitero, M. V.C. Corrente, J. E. Nunes, H. R.C. Candido, A. F. Costa, S. M.B. Melo, J. V.F. Reis, F. V.D.S. Garcia, G. A. Rabadan, G. B. Bassin, H. C.M. Suyama, K. S. Damasceno, L. N. Takemoto, M. L.S. Menezes, M. D. Bussaneli, D. G. Nogueira, V. K.C. Lima, P. R. Lourenço, I. O. Marostica De Sá, J. Megid, R. A. Caruso, I. P. Rasmussen, L. T. Prata, G. M. Arantes, M. A. Ferraz, G. A.R. Camargo, L. P. Kron, M. R. |
dc.subject.por.fl_str_mv |
Collagen Electromyography Gestational diabetes mellitus Hyperglycemic myopathy Pelvic floor muscles Proteomics Rectus abdominis muscles Transmission electron microscopy Urinary incontinence |
topic |
Collagen Electromyography Gestational diabetes mellitus Hyperglycemic myopathy Pelvic floor muscles Proteomics Rectus abdominis muscles Transmission electron microscopy Urinary incontinence |
description |
Background: Pelvic floor muscles (PFM) and rectus abdominis muscles (RAM) of pregnant diabetic rats exhibit atrophy, co-localization of fast and slow fibers and an increased collagen type I/III ratio. However, the role of similar PFM or RAM hyperglycemic-related myopathy in women with gestational diabetes mellitus (GDM) remains poorly investigated. This study aims to assess the frequency of pelvic floor muscle disorders and pregnancy-specific urinary incontinence (PS-UI) 12 months after the Cesarean (C) section in women with GDM. Specifically, differences in PFM/RAM hyperglycemic myopathy will be evaluated. Methods: The Diamater is an ongoing cohort study of four groups of 59 pregnant women each from the Perinatal Diabetes Research Centre (PDRC), Botucatu Medical School (FMB)-UNESP (São Paulo State University), Brazil. Diagnosis of GDM and PS-UI will be made at 24-26 weeks, with a follow-up at 34-38 weeks of gestation. Inclusion in the study will occur at the time of C-section, and patients will be followed at 24-48 h, 6 weeks and 6 and 12 months postpartum. Study groups will be classified as (1) GDM plus PS-UI; (2) GDM without PS-UI; (3) Non-GDM plus PS-UI; and (4) Non-GDM without PS-UI. We will analyze relationships between GDM, PS-UI and hyperglycemic myopathy at 12 months after C-section. The mediator variables to be evaluated include digital palpation, vaginal squeeze pressure, 3D pelvic floor ultrasound, and 3D RAM ultrasound. RAM samples obtained during C-section will be analyzed for ex-vivo contractility, morphological, molecular and OMICS profiles to further characterize the hyperglycemic myopathy. Additional variables to be evaluated include maternal age, socioeconomic status, educational level, ethnicity, body mass index, weight gain during pregnancy, quality of glycemic control and insulin therapy. Discussion: To our knowledge, this will be the first study to provide data on the prevalence of PS-UI and RAM and PFM physical and biomolecular muscle profiles after C-section in mothers with GDM. The longitudinal design allows for the assessment of cause-effect relationships between GDM, PS-UI, and PFMs and RAMs myopathy. The findings may reveal previously undetermined consequences of GDM. |
publishDate |
2020 |
dc.date.none.fl_str_mv |
2020-12-12T01:58:39Z 2020-12-12T01:58:39Z 2020-02-19 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1186/s12884-020-2749-x BMC Pregnancy and Childbirth, v. 20, n. 1, 2020. 1471-2393 http://hdl.handle.net/11449/200135 10.1186/s12884-020-2749-x 2-s2.0-85081144374 9162508978945887 0000-0003-2460-1145 |
url |
http://dx.doi.org/10.1186/s12884-020-2749-x http://hdl.handle.net/11449/200135 |
identifier_str_mv |
BMC Pregnancy and Childbirth, v. 20, n. 1, 2020. 1471-2393 10.1186/s12884-020-2749-x 2-s2.0-85081144374 9162508978945887 0000-0003-2460-1145 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
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BMC Pregnancy and Childbirth |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
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UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
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1808128126149984256 |