An experimental model for the study of collagen fibers in skeletal muscle

Detalhes bibliográficos
Autor(a) principal: Calvi,Eliziane Nitz de Carvalho
Data de Publicação: 2012
Outros Autores: Nahas,Fábio Xerfan, Barbosa,Marcus Vinícius, Calil,José Augusto, Ihara,Silvia Saiuli Miki, Silva,Marcelo de Souza, Franco,Marcello Fabiano de, Ferreira,Lydia Masako
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Acta Cirúrgica Brasileira (Online)
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-86502012001000003
Resumo: PURPOSE: To examine histological and histomorphometric techniques for measuring collagen in skeletal muscle. METHODS: The following staining methods were used in the study: hematoxylin and eosin, Masson's trichrome, reticulin, and picrosirius red, and immunostaining for collagen types I, II, III, IV, and V. Histomorphometric measurements were performed using Corel PhotoPaint and UTHSCSA Image Tool 3.0 software. RESULTS: Both the Masson's trichrome and picrosirius red staining provided the best visualization for the measurement of collagen content. CONCLUSION: This methodology is important for the identification and quantification of the different types of collagen in muscles and can be used in the investigation of the qualitative and quantitative influence of collagen on physical activities, aging, and diseases.
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spelling An experimental model for the study of collagen fibers in skeletal muscleCollagenMuscle, SkeletalHistologyRectus AbdominisCadaverPURPOSE: To examine histological and histomorphometric techniques for measuring collagen in skeletal muscle. METHODS: The following staining methods were used in the study: hematoxylin and eosin, Masson's trichrome, reticulin, and picrosirius red, and immunostaining for collagen types I, II, III, IV, and V. Histomorphometric measurements were performed using Corel PhotoPaint and UTHSCSA Image Tool 3.0 software. RESULTS: Both the Masson's trichrome and picrosirius red staining provided the best visualization for the measurement of collagen content. CONCLUSION: This methodology is important for the identification and quantification of the different types of collagen in muscles and can be used in the investigation of the qualitative and quantitative influence of collagen on physical activities, aging, and diseases.Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia2012-10-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-86502012001000003Acta Cirúrgica Brasileira v.27 n.10 2012reponame:Acta Cirúrgica Brasileira (Online)instname:Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia (SBDPC)instacron:SBDPC10.1590/S0102-86502012001000003info:eu-repo/semantics/openAccessCalvi,Eliziane Nitz de CarvalhoNahas,Fábio XerfanBarbosa,Marcus ViníciusCalil,José AugustoIhara,Silvia Saiuli MikiSilva,Marcelo de SouzaFranco,Marcello Fabiano deFerreira,Lydia Masakoeng2012-09-27T00:00:00Zoai:scielo:S0102-86502012001000003Revistahttps://www.bvs-vet.org.br/vetindex/periodicos/acta-cirurgica-brasileira/https://old.scielo.br/oai/scielo-oai.php||sgolden@terra.com.br0102-86501678-2674opendoar:2012-09-27T00:00Acta Cirúrgica Brasileira (Online) - Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia (SBDPC)false
dc.title.none.fl_str_mv An experimental model for the study of collagen fibers in skeletal muscle
title An experimental model for the study of collagen fibers in skeletal muscle
spellingShingle An experimental model for the study of collagen fibers in skeletal muscle
Calvi,Eliziane Nitz de Carvalho
Collagen
Muscle, Skeletal
Histology
Rectus Abdominis
Cadaver
title_short An experimental model for the study of collagen fibers in skeletal muscle
title_full An experimental model for the study of collagen fibers in skeletal muscle
title_fullStr An experimental model for the study of collagen fibers in skeletal muscle
title_full_unstemmed An experimental model for the study of collagen fibers in skeletal muscle
title_sort An experimental model for the study of collagen fibers in skeletal muscle
author Calvi,Eliziane Nitz de Carvalho
author_facet Calvi,Eliziane Nitz de Carvalho
Nahas,Fábio Xerfan
Barbosa,Marcus Vinícius
Calil,José Augusto
Ihara,Silvia Saiuli Miki
Silva,Marcelo de Souza
Franco,Marcello Fabiano de
Ferreira,Lydia Masako
author_role author
author2 Nahas,Fábio Xerfan
Barbosa,Marcus Vinícius
Calil,José Augusto
Ihara,Silvia Saiuli Miki
Silva,Marcelo de Souza
Franco,Marcello Fabiano de
Ferreira,Lydia Masako
author2_role author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Calvi,Eliziane Nitz de Carvalho
Nahas,Fábio Xerfan
Barbosa,Marcus Vinícius
Calil,José Augusto
Ihara,Silvia Saiuli Miki
Silva,Marcelo de Souza
Franco,Marcello Fabiano de
Ferreira,Lydia Masako
dc.subject.por.fl_str_mv Collagen
Muscle, Skeletal
Histology
Rectus Abdominis
Cadaver
topic Collagen
Muscle, Skeletal
Histology
Rectus Abdominis
Cadaver
description PURPOSE: To examine histological and histomorphometric techniques for measuring collagen in skeletal muscle. METHODS: The following staining methods were used in the study: hematoxylin and eosin, Masson's trichrome, reticulin, and picrosirius red, and immunostaining for collagen types I, II, III, IV, and V. Histomorphometric measurements were performed using Corel PhotoPaint and UTHSCSA Image Tool 3.0 software. RESULTS: Both the Masson's trichrome and picrosirius red staining provided the best visualization for the measurement of collagen content. CONCLUSION: This methodology is important for the identification and quantification of the different types of collagen in muscles and can be used in the investigation of the qualitative and quantitative influence of collagen on physical activities, aging, and diseases.
publishDate 2012
dc.date.none.fl_str_mv 2012-10-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-86502012001000003
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-86502012001000003
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S0102-86502012001000003
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia
publisher.none.fl_str_mv Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia
dc.source.none.fl_str_mv Acta Cirúrgica Brasileira v.27 n.10 2012
reponame:Acta Cirúrgica Brasileira (Online)
instname:Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia (SBDPC)
instacron:SBDPC
instname_str Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia (SBDPC)
instacron_str SBDPC
institution SBDPC
reponame_str Acta Cirúrgica Brasileira (Online)
collection Acta Cirúrgica Brasileira (Online)
repository.name.fl_str_mv Acta Cirúrgica Brasileira (Online) - Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia (SBDPC)
repository.mail.fl_str_mv ||sgolden@terra.com.br
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